Investigation of Stem Cell Applications on In Vitro Fertilization in Rats


Keywords:
Bone Marrow Mesenchymal Stem Cell In Vitro Fertilization Oocyte Sperm Superovulation
A GUMURDU
S OZTURK
I AYDEMIR
MI TUGLU
Abstract

We aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tubal Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos was followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos reached 2, 4, 8, 16 cells stages and morula stage in the CM. While AMM had a negative effect on fertilization and embryo development, the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shown that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryos.

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References
Agarwal A, Majzoub A, Parekh N, et al. (2020) Schematic Overview of the Current Status of Male Infertility Practice. World J Mens Health 38(3):308-322.
Akbari H, Eftekhar Vaghefi SH, et al. (2017) Mesenchymal Stem Cell-Conditioned Medium Modulates Apoptotic and Stress-Related Gene Expression, Ameliorates Maturation and Allows for the Development of Immature Human Oocytes after Artificial Activation. Genes (Basel) 8;8(12):371.
Aydemir I, Özkut MM, Fırat F, et al. (2018) Effects of stem cells applications on oxidative stress and apoptosis during implantation. Asian Pac J Reprod 2018;7:229-35.
Bader P, Kuci Z, Bakhtiar S, et al. (2018) Effective treatment of steroid and therapy-refractory acute graft-versus-host disease with a novel mesenchymal stromal cell product (MSC-FFM). Bone Marrow Transplant 53(7): 852-862.
Bhardwaj G, Mulligan VK, Bahl CD, et al. (2016) Accurate de novo design of hyperstable constrained peptides. Nature 20;538(7625):329-335.
Bukovsky A, Caudle MR, Svetlikova M, et al. (2004) Origin of germ cells and formation of new primary follicles in adult human ovaries. Reprod. Biol. Endocrinol 2:20.
Cakici C, Buyrukcu B, Duruksu G, et al. (2013) Recovery of fertility in azoospermia rats after injection of adipose-tissue-derived mesenchymal stem cells: the sperm generation. Biomed. Res. Int 529589:doi:10.1155/2013/529589.
Cakina S, Ocak O, Ozkan A, et al. (2018) Vitamin D receptor gene polymorphisms in multiple sclerosis disease: A case-control study. Revista Română de Medicină de Laborator 26:doı:10.2478/rrlm-2018-0028.
Clarke GN. (2006) Association between sperm autoantibodies and enhanced embryo implantation rates during in vitro fertilization. Fertil. Steril 86(3):753-4.
Cuman C, Menkhorst EM, Rombauts LJ, et al. (2013) Preimplantation human blastocysts release factors that differentially alter human endometrial epithelial cell adhesion and gene expression relative to IVF success. Hum. Reprod 28(5):1161-71.
de Oliveira Bezerra A, Nicacio AC, de Oliveira Menezes GR, et al. (2019) Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves. Anim Reprod Sci 208:106101.
Del Giudice F, Kasman AM, Ferro M, Sciarra A, De Berardinis E, Belladelli F, Salonia A, Eisenberg ML. Clinical correlation among male infertility and overall male health: A systematic review of the literature. Investig Clin Urol. 2020 Jul;61(4):355-371.
Demeestere I, Centner J, Gervy C, et al. (2005) Impact Of Various Endocrine And Paracrine Factors on In Vitro Culture of Preantral Follicles In Rodents. Reproduction 130:147-156.
Dominici M, Hofmann TJ, Horwitz EM (2001) Bone marrow mesenchymal stem cells: biological properties and clinical application. J. Biol. Reg. Homeo. Ag 15:28-37.
Estuardo LIJ, Carlos DM, Alejandro KB, et al. (2020) Thrombophilias and the risk of recurring pregnancy loss in a Mexican population. Obstet Gynecol Int J 11(6):342‒346.
Feng DQ, Zhou Y, Ling B, et al. (2009) Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development. Braz. J. Med. Biol. Res 42(6):506-14.
Gunes S, Sengupta P, Henkel R, Alguraigari A, Sinigaglia MM, Kayal M, Joumah A, Agarwal A. Microtubular Dysfunction and Male Infertility. World J Mens Health. 2020 Jan;38(1):9-23.
Honda A, Tachibana R, Hamada K, et al. (2019) Efficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization. Sci Rep 9;11571.
Jia Y, Li S, Wang Y (2016) Research And Prospect Of Mesenchymal Stem Cells In Female Fertılıty Preservatıon, Zhongguo. Xiu. Fu. Chong. Jian. Wai. Ke. Za. Zhi 30(3):314-9.
Jin HF, Kumar BM, Kim JG, et al. (2016) Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells. Int. J. Dev. Biol 51(1):85-90.
Karimaghai N, Tamadon A, Rahmanifar F, Mehrabani D, Raayat Jahromi A, Zare S, Khodabandeh Z, Razeghian Jahromi I, Koohi-Hoseinabadi O, Dianatpour M. Spermatogenesis after transplantation of adipose tissue-derived mesenchymal stem cells in busulfan-induced azoospermic hamster. Iran J Basic Med Sci. 2018 Jul;21(7):660-667.
Kumar S (2014) Bone defect repair in mice by mesenchymal stem cells. Methods. Mol. Biol 1213:193-207.
Ling B, Feng DQ, Zhou Y, et al. (2008) Effect of conditioned medium of mesenchymal stem cells on the in vitro maturation and subsequent development of mouse oocyte. Braz. J. Med. Biol. Res 41(11):978-85.
Ma Q, Wawersik M, Matunis EL. (2014) The Jak-STAT Target Chinmo Prevents Sex Transformation of Adult Stem Cells in the Drosophila Testis Niche. Dev. Cell 31(4): 474-486.
Manuel VH. (2020) Autologous Mesenchimal Stem Cell Therapy in Patients with Unexplainable Low Ovarian Response. J Gynecol Res Rev Rep SRC/JGRRR-119. 2020:3.
Mirnamniha M, Faroughi F, Tahmasbpour E et al. (2019) An overview on role of some trace elements in human reproductive health, sperm function and fertilization process. Rev Environ Health 34:339-348.
Mokarizadeh A, Rezvanfar MA, Dorostkar K, et al. (2013) Mesenchymal stem cell derived microvesicles: trophic shuttles for enhancement of sperm quality parameters. Reprod. Toxicol 42:78-84.
Montella KR, Keely E, Laifer SA, et al. (2000) Evaluation and management of infertility in women: The internist’role. Annals of Internal medicine 132:973-981.
Mozafar A, Mehrabani D, Vahdati A, et al. (2018) Histomorphometric Evaluation of Allogeneic Transplantation of Bone Marrow Mesenchymal Stem Cells in Azoospermic Mice Model. The Inodenesian Biomedical Journal 10(2):171-178.
Ng EH, Lau EY, Yeung WS, et al (2003) Oocyte and embryo quality in patients with excessive ovarian response during in vitro fertilization treatment. J. Assist. Reprod. Genet 20:186-91.
Noverina R, Widowati W, Ayuningtyas W, et al. (2019) Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (cm-hatmscs). Clinical Nutrition Experimental 24:34-44.
Opiela J, Samiec M (2013) Characterization of mesenchymal stem cells and their application in experimental embryology. Pol. J. Vet. Sci 16:593-9.
Osman E, Franasiak J, Scott R. Oocyte and Embryo Manipulation and Epigenetics. Semin Reprod Med. 2018 May;36(3-04):e1-e9.
Pittenger MF, Mackay AM, Beck SC, et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147.
Ullah I, Subbarao RB, Rho GJ. (2015) Human mesenchymal stem cells - current trends and future prospective. Bioscience reports 35(2), e00191.
Virant-Klun I, Omejec S, Stimpfel M, et al. (2019) Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme. Stem Cell Rev Rep 15(4):543-557.
Wasilewski T, Łukaszewicz-Zając M, Wasilewska J, et al. (2020). Biochemistry of infertility. Clin Chim Acta 508:185-190.
Xia X, Wang T, Yin T, et al. (2015) Mesenchymal Stem Cells Facilitate In Vitro Development of Human Preantral Follicle. Reproductive Sciences 22:1367-1376.
Yang P, Humphrey SJ, Cinghu S, et al. (2019) Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency. Cell Syst 22;8(5):427-445.e10.
Yao C, Zhang W, Shuai L. (2019) The first cell fate decision in pre-implantation mouse embryos. Cell Regen 20;8(2):51-57.
Zhou S, Le Boff MS, Glowacki J. (2010) Vitamin D metabolism and action in human bone marrow stromal cells. Endocrinology 151(1):14-22.
Zhu Z, Zhang Y, Wu L, et al. (2020) Regeneration-Related Functional Cargoes in Mesenchymal Stem Cell-Derived Small Extracellular Vesicles. Stem Cells Dev 1;29(1):15-24
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