GDF9 gene c.260G>A mutation and sheep litter size: a meta-analysis

PDF (English)
Veröffentlicht: Jul 15, 2024
Aktualisiert: 2024-07-15
Versionen:
2024-07-15 (2)
DOI: https://doi.org/10.12681/jhvms.35128
MR Sahib
Department of Horticulture, College of Agriculture, Al-Qasim Green University, Babil 51001, Iraq
IL AL-Jaryan
Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil 51001, Iraq.
TM Al-Thuwaini
Department of Animal Production, College of Agriculture, Al-Qasim Green University, Babil 51001, Iraq
Abstract

Growth differentiation factor 9 (GDF9) has been identified as a major gene associated with sheep litter size. The most frequent single nucleotide polymorphism (SNP) associated with GDF9 is c.260G>A, altering protein sequence. Some studies found that this SNP did not affect litter size, while others found that it had an effect. This study examined the impact of this polymorphism on litter size through a meta-analysis. An analysis was conducted on six eligible published studies to examine the effects of c.260G>A polymorphism on litter size using four different genetic models: dominant (GG + GA versus AA), recessive (GG versus GA + AA), additive (GG versus AA) and co-dominant (GG + AA versus GA). The data were analyzed according to the I-squared value using fixed-effects and random-effects models. The c.260G>A polymorphism affected litter size significantly when analyzed as recessive (SMD = -0.24, 95 % CI [-0.41, -0.08]) and co-dominant (SMD = -0.36, 95 % CI [-0.64, -0.07]). In contrast, the c.260G>A polymorphism had no effect when used with dominant (SMD = 0.35, 95 % CI [-0.26, 0.96]) and additive (SMD = 0.18, 95 % CI [-0.78, 1.15]) genetic models. A statistical sensitivity analysis of pooled SMDs revealed no differences, indicating the overall results did not result from a single study. A meta-analysis shows that sheep litter size is related to genotype GA. This study supported the concept that GDF9 is a fundamental factor that influences sheep litter size.

Article Details
  • Rubrik
  • Research Articles
Creative-Commons-Lizenz
Dieses Werk steht unter der Lizenz Creative Commons Namensnennung - Nicht-kommerziell 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Keine Nutzungsdaten vorhanden.
Literaturhinweise
Abdelgadir AZ, Musa LM, Jawasreh KI, Saleem AO, El-Hag F, Ahmed
MKA (2021) G1 point mutation in growth differentiation factor 9
gene affects litter size in Sudanese desert sheep. Vet World 14(1): 104.
https://doi.org/10.14202/vetworld.2021.104-112
Ajafar MH, Al-Thuwaini TM, Dakhel HH (2022a) Association of OLR1
gene polymorphism with live body weight and body morphometric
traits in Awassi ewes. Mol Biol Rep 49(5): 4149-4153. https://doi.
org/10.1007/s11033-022-07481-3
Ajafar MH, Kadhim AH, AL-Thuwaini TM (2022b) The reproductive
traits of sheep and their influencing factors. Rev Agric Sci 10: 82-89.
https://doi.org/10.7831/ras.10.0_82
Ali MA, Kadhim AH, Al-Thuwaini TM (2022) Genetic variants of the
bone morphogenetic protein gene and its association with estrogen
and progesterone levels with litter size in Awassi ewes. Iraqi J Vet Sci
(4): 1017-1022. https://doi.org/10.33899/IJVS.2022.132903.2143
AL-Jaryan IL, AL-Thuwaini TM, Merzah LH, Alkhammas AH (2023)
Reproductive Physiology and Advanced Technologies in Sheep
Reproduction. Rev Agric Sci 11: 171-180. https://doi.org/10.7831/
ras.11.0_171
Al-Jumaili WS, Kadhim AH, Al-Thuwaini TM (2023) Polymorphism of the ADIPOQ gene and its association with productive traits in Awassi
Ewes. Mol Biol Rep 50(1): 913-917. https://doi.org/10.1007/s11033-
-07975-0
Alkass JE, Hermiz HN, Baper MI (2021) Some aspects of reproductive
efficiency in awassi ewes: A review. Iraqi J Agric Sci 52(1): 20-27.
Al-Khuzai FLJ, Ahmed JR (2019) Polymorphism of GDF9 (exon-1) gene
and its association with milk production and prolificacy of Awassi
sheep. Plant Arch 19(2): 4037-4040.
Al-Mutar HA, Younis LS (2020) Effect of point mutation in the growth
differentiation factor 9 gene of oocytes on the sterility and fertility
of Awassi sheep. Arch Razi Inst 75(1): 101. https://doi.org/10.22092/
ARI.2018.122232.1220
Al-Thuwaini TM, Al-Hadi ABA (2022) Association of lamb sex with
body measurements in single and twin on the Awassi ewes. Adv
Anim Vet Sci 10(8): 1849-1853. https://dx.doi.org/10.17582/journal.
aavs/2022/10.8.1849.1853
Bayraktar M, Özdemir M (2022) A meta-analysis of the association
between Growth Hormone (GH) gene polymorphism and growth
traits in cattle breeds. J Hellenic Vet Med Soc 73(3). https://dx.doi.
org/10.12681/jhvms.29407
Bayraktar M, Shoshin O (2022a) Association between CAST and MSTN
gene polymorphisms with growth traits in Awassi sheep. Kuwait J Sci
(2). https://dx.doi.org/10.48129/kjs.10955
Bayraktar M, Shoshin O (2022b) Estimation of the associations between
GH and DGAT1 genes and growth traits by using decision tree in
Awassi sheep. Anim Biotechnol 33(1): 167-173. https://doi.org/10.10
/10495398.2021.1975727
Gorlov IF, Kolosov YA, Shirokova NV, Getmantseva LV, Slozhenkina MI,
Mosolova NI, Bakoev NF, Leonova MA, Kolosov AY, Zlobina EY
(2018) GDF9 gene polymorphism and its association with litter size
in two Russian sheep breeds. Rend Fis Acc Lincei 29: 61-66. https://
doi.org/10.1007/s12210-017-0659-2
Hanrahan JP, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R,
Galloway SM (2004) Mutations in the genes for oocyte-derived
growth factors GDF9 and BMP15 are associated with both increased
ovulation rate and sterility in Cambridge and Belclare sheep (Ovis
aries). Biol Reprod 70(4): 900-909.
Higgins JP, Thomas J, Chandler J, Cumpston M, Li T, Page MJ, Welch VA
(2019) Cochrane handbook for systematic reviews of interventions.
John Wiley & Sons. https://doi.org/10.1095/biolreprod.103.023093
Hossain MI, Khan MKI, Momin MM, Das A (2020) Effects of protein
supplements on fertility and assessment of the fertility genes (GDF9
and BMP15) in indigenous sheep of Bangladesh. J Appl Anim Res
(1): 484-491. https://doi.org/10.1080/09712119.2020.1830779
Kadhem AF, Al-Thuwaini TM (2022) Influence of litter size on the hematologic profile of Awassi ewes during gestation and lactation. Vet
Integr Sci 20(3): 625-633. https://doi. org/10.12982/VIS. 2022.047
Khodabakhshzadeh R, Mohammadabadi MR, Esmailizadeh AK, Shahrebabak HM, Bordbar F, Namin SA (2016) Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Pol J Vet Sci 19(2):
-289. https://doi.org/10.1515/pjvs-2016-0035
Mahmoudi P, Rashidi A, Rostamzadeh J, Razmkabir M (2019) Association between c. 1189G> A single nucleotide polymorphism of GDF9
gene and litter size in goats: A meta-analysis. Anim Reprod Sci 209:
https://doi.org/10.1016/j.anireprosci.2019.106140
Medrado BD, Pedrosa VB, Pinto LFB (2021) Meta-analysis of genetic parameters for economic traits in sheep. Livest Sci 247: 104477. https://
doi.org/10.1016/j.livsci.2021.104477
Mohamed SEI, Ahmed RM, Jawasreh KI, Salih MAM, Abdelhalim DM,
Abdelgadir AW, Obeidat MT, Musa LMA, Ahmed MKA (2020)
Genetic polymorphisms of fecundity genes in Watish Sudanese desert sheep. Vet World 13(4): 614-621. https://doi.org/10.14202/vetworld.2020.614-621
Moradband F, Rahimi G, Gholizadeh M (2011) Association of polymorphisms in fecundity genes of GDF9, BMP15 and BMP15-1B with
litter size in Iranian Baluchi sheep. Asian-Australas J Anim Sci 24(9):
-1183. http://dx.doi.org/10.5713/ajas.2011.10453
Muhaghegh Dolatabady M, Habibizad J (2019) Single nucleotide polymorphisms (SNPs) of GDF9 gene in Bahmaei and Lak Ghashghaei
sheep breeds and its association with litter size. Iran J Appl Anim Sci
(3): 427-432.
Putra WPBP, Margawati ET, Pardede S, Soetrisno E, Raadsma HW (2022)
Detection of G1 point mutation (c. 2039G> A) in GDF9 gene of
MEGA (Merino× Garut) sheep. Veterinaria 71(3): 357-364. https://
doi.org/10.51607/22331360.2022.71.3.357
Rahmawati RY, Sumadi S, Hartatik T (2019) Identification of single
nucleotide polymorphisms in GDF9 gene associated with litter
size in Garut sheep. Indones J Biotechnol 24(1): 51-56. https://doi.
org/10.22146/ijbiotech.42095
Talebi R, Ahmadi A, Afraz F, Sarry J, Woloszyn F, Fabre S (2018) Detection of single nucleotide polymorphisms at major prolificacy genes
in the Mehraban sheep and association with litter size. Ann Anim Sci
(3): 685-698. https://doi.org/10.2478/aoas-2018-0014
Wang F, Chu M, Pan L, Wang X, He X, Zhang R, Tao L, La Y, Ma L, Di
R (2021) Polymorphism detection of GDF9 gene and its association
with litter size in Luzhong mutton sheep (Ovis aries). Animals 11(2):
https://doi.org/10.3390/ani11020571
Zhang Z, Sui Z, Zhang J, Li Q, Zhang Y, Wang C, Li X, Xing F (2022)
Identification of Signatures of Selection for Litter Size and Pubertal
Initiation in Two Sheep Populations. Animals 12(19): 2520. https://
doi.org/10.3390/ani12192520