Determination of Different Types of Milk in Commercially Sold Goat Cheeses by Real-Time PCR Method

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Veröffentlicht: Ιαν 15, 2025
DOI: https://doi.org/10.12681/jhvms.36885
RM Tuncay
Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 65040, Van, Türkiye
YC Sancak
Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology
Abstract

Gıdaların yanlış etiketlenmesi, özellikle menşei belirlenmiş birinci sınıf ürünler için tüketici haklarını ve bilinçli seçimleri etkiler. İçerik ikameleri kaliteyi düşürebilir, kimliği sulandırabilir ve adil rekabeti bozabilir. Gelişmiş ülkeler bu sorunla mücadele etmek için katı ulusal ve uluslararası düzenlemeler uygulamaktadır. Bu çalışmada RT-PCR yöntemi kullanılarak "%100 Keçi Sütü" olarak etiketlenen peynirlerde ve koyun sütünün varlığının doğru ve güvenilir bir şekilde tespit edilmesi amaçlanmıştır. Marketlerden "%100 Keçi Sütü" etiketli farklı üretim tarihlerine ve parti numaralarına sahip 100 peynir numunesi toplandı. Örneklerde, mitokondriyal DNA'da türe özgü bölgeyi kalitatif olarak tespit etmek için Real-Time PCR TaqMan prob yöntemi kullanılmış ve tür düzeyinde ayrım yapılmıştır. Çalışmada "%100 Keçi Sütü" etiketi ile etiketlenen peynir örneklerinin %76'sının (100 peynirden 76'sı) etikette yer alan ifadeye uymadığı tespit edilmiştir. Peynir örneklerinin %27'sinde (27) sadece sütü, %4'ünde (4) sadece koyun sütü, %9'unda keçi ve sütü, %9'unda (9), %16'sında ve koyun sütü, %17'sinde ise keçi, koyun ve sütü tespit edilmiştir (17). Çalışma, keçi sütü içeren peynirlerin önemli ölçüde tağşiş edildiğini ortaya koymakta ve üretim ve satış sırasında titiz bir izleme ihtiyacını vurgulamaktadır. Sonuç olarak, Real-Time PCR yöntemi, keçi peynirlerinde düşük koyun ve sütü düzeylerini tespit etme yeteneği ile etkili bir tanı yöntemi olarak önerilmektedir.

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Autor/innen-Biografie
YC Sancak, Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology

 Prof.Dr. Yakup Can SANCAK. Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi Anabilim Dalı

 

Literaturhinweise
Agrimonti C, Pirondini A, Marmiroli M, Marmiroli N (2015) A quadruplex
PCR (qxPCR) assay for adulteration in dairy products. Food
Chemistry 187: 58-64.
Bania J, Ugorski M, Polanowski A, Adamczyk E (2001) Application of
polymerase chain reaction for detection of goats’ milk adulteration by
milk of cow. J Dairy Res 68:333-336.
Bottero MT, Civera T, Nucera D, Rosati S, Sacchi P, Turi RM (2003) A
multiplex polymerase chain reaction for the identification of cows’,
goats’ and sheep’s milk in dairy products. Int Dairy J 13:277-282.
Calvo JH, Osta R, Zaragoza P (2002). Quantitative PCR detection of pork
in raw and heated groundbeef and pâté. J Agric Food Chem 50:5265-
Chávez NA, Salinas E, Jauregui J, Palomares LA, Macías K (2008) Detection
of bovine milk adulterated with cheese whey by western blot
immunoassay. Food Agr Immunol 19:265-272.
Chen RK, Chang LW, Chung YY, Lee MH, Ling YC (2004) Quantification
of cow milk adulteration in goat milk using high-performance
liquid chromatography with electrospray ionization mass spectrometry.
Rapid Commun Mass Spectrom 18:1167-1171.
Cheng YH, Chen SD, Weng CF (2006) Investigation of Goats??Milk
Adulteration with Cows?? Milk by PCR. Asian-Austral J Animal
:1503-1507.
Cottenet G, Blancpain C, Golay PA (2011) Simultaneous detection of cow
and buffalo species in milk from China, India, and Pakistan using
multiplex real-time PCR. J Dairy Sci 94:3787-3793.
Dąbrowska A, Wałecka E, Bania J, Żelazko M, Szołtysik M, Chrzanowska
J (2010) Quality of UHT goat’s milk in Poland evaluated by real-
time PCR. Small Ruminant Res 94:32-37.
Darwish SF, Allam A, Amin AS (2009) Evaluation of PCR Assay for Detection
of Cow’s Milk in Water Buffalo’s Milk. WASJ 7: 461-467.
Di Domenico M, Di Giuseppe M, Wicochea Rodríguez JD, Cammà C
(2017) Validation of a fast real-time PCR method to detect fraud and
mislabeling in milk and dairy products J Dairy Sci, 100:106-112.
Di Pinto A, Terio V, Marchetti P, Bottaro M, Mottola A, Bozzo G, Bonerba
E, Ceci E, Tantillo G (2017) DNA-based approach for species identification
of goat-milk products. Food Chemistry 229: 93-97.
Dias LA, Peres AM, Veloso ACA, Reis FS, Vilas-Boas M, Machado
AASC (2009) An electronic tongue taste evaluation: Identification of
goat milk adulteration with bovine milk. Sensor Actuat B Chem 136:
-217.
EC (2002) Regulation (EC) No 178/2002 of the European Commission, which
establishes standards for food safety and traceability and mandates food
businesses to adhere, holds significant importance https://wwwgooglecom/
search?q=Regulation+(EC)+No+178%2F2002+of+the+European+
Commission%2C+which+establishes+standards+-
for+food+safety+and+traceability+and+mandates+food+businesses+
to+adhere%2C+holds+significant+importanceandoq=-
Regulation+(EC)+No+178%2F2002+of+the+European+Commission%
C+which+establishes+standards+for+food+safety+
and+traceability+and+mandates+food+businesses+to+adhere%
C+holds+significant+importanceandgs_lcrp=EgZjaHJvb-
WUyBggAEEUYOdIBBzQxMGowajGoAgCwAgAandsourceid=-
chromeandie=UTF-8 [accessed 23 October 2023].
EC (2015) Ff_ffn_annual-report_2015pdf from https://foodeceuropaeu/
system/files/2020-05/ff_ffn_annual-report_2015pdf [accessed 23 October
.
EC (2019) https://foodeceuropaeu/safety/eu-agri-food-fraud-network_en
[accessed 19 October 2023].
Everstine K, Spin, J, Kennedy S (2013) Economically Motivated Adulteration
(EMA) of Food: Common Characteristics of EMA Incidents. J
Food Protect 76:723-735.
Ferreira IMPLVO, Caçote H (2003) Detection and quantification of bovine,
ovine and caprine milk percentages in protected denomination
of origin cheeses by reversed-phase high-performance liquid chromatography
of beta-lactoglobulins. J Chromatogr A 1015:111-118.
Ghovvati S, Nassiri MR, Mirhoseini SZ, Moussavi AH, Javadmanesh A
(2009) Fraud identification in industrial meat products by multiplex
PCR assay. Food Control 20:696-699.
Golinelli LP, Carvalho AC, Casaes RS, Lopes CSC, Deliza R, Paschoalin
VMF, Silva JT (2014) Sensory analysis and species-specific PCR
detect bovine milk adulteration of frescal (fresh) goat cheese. J Dairy
Sci 97:6693-6699.
Gonçalves J, Pereira F, Amorim A, van Asch B (2012) New Method for
the Simultaneous Identification of Cow, Sheep, Goat, and Water Buffalo
in Dairy Products by Analysis of Short Species-Specific Mitochondrial
DNA Targets. J Agr Food Chem 60:10480-10485.
Haenlein GFW (2004) Goat milk in human nutrition Small Ruminant Res
:155-163.
Haunshi S, Basumatary R, Girish PS, Doley S, Bardoloi RK, Kumar A
(2009) Identification of chicken, duck, pigeon and pig meat by species-
specific markers of mitochondrial origin. Meat Science 83:454-
Herman L (2001) Determination of the animal origin of raw food by species-
specific PCR. J Dairy Res 68:429-436.
Hurley IP, Elyse Ireland H, Coleman RC, Williams JHH (2004) Application
of immunological methods for the detection of species adulteration
in dairy products. Int J Food Sci Tech , 39:873-878.
Khanzadi S, Jamshidi A, Razmyar J, Mohsenzadeh M (2014) PCR-based
detection of cow and goat milk in sheep milk and dairy products marketed
in Mashhad city of Iran. Iran J Vet Med, 7:257-262.
Kotowicz M, Adamczyk E, Bania J (2007) Application of a duplex-PCR
for detection of cows’ milk in goats’ milk. Ann Agr Env Med 4.
López-Calleja I, Alonso IG, Fajardo V, Rodríguez MA, Hernández PE,
García T, Martín R (2005) PCR detection of cows’ milk in water buffalo
milk and mozzarella cheese. Int Dairy J 15:1122-1129.
López-Calleja IM, González I, Fajardo V, Hernández PE, García T, Martín
R (2007) Application of an indirect ELISA and a PCR technique for
detection of cows’ milk in sheep’s and goats’ milk cheeses. Int Dairy
J 17:87-93.
Mašková E, Paulíčková I (2006) PCR-based detection of cow’s milk in
goat and sheep cheeses marketed in the Czech Republic. Czech J
Food Sci 4:127.
Mayer HK (2005) Milk species identification in cheese varieties using
electrophoretic, chromatographic and PCR techniques. Int Dairy J
:595-604.
Moatsou G, Anifantakis E (2003) Recent developments in antibody-based
analytical methods for the differentiation of milk from different species.
Int J Dairy Techn 56:133-138.
Moyer DC, DeVries JW, Spink J (2017) The economics of a food fraud
incident-Case studies and examples including Melamine in Wheat
Gluteni Food Control 71:358-364.
Pesic M, Barac M, Vrvic M, Ristic N, Macej O, Stanojevic S (2011) Qualitative
and quantitative analysis of bovine milk adulteration in caprine
and ovine milks using native-PAGE. Food Chemistry 125:1443-1449.
Rahmati S, Julkapli NM, Yehye WA, Basirun WJ (2016) Identification
of meat origin in food products-A review. Food Control 68:379-390.
Rodrigues NPA, Givisiez PEN, Queiroga RCRE, Azevedo PS, Gebreyes
WA, Oliveira, CJB (2012) Milk adulteration: Detection of bovine
milk in bulk goat milk produced by smallholders in northeastern Brazil
by a duplex PCR assay. J Dairy Sci, 95:2749-2752.
Rodríguez MA, García T, González I, Asensio L, Hernández PE, Martín R
(2004) PCR Identification of Beef, Sheep, Goat, and Pork in Raw and
Heat-Treated Meat Mixtures. J Food Protect 67:172-177.
Rodríguez-Ramírez R, González-Córdova AF, Vallejo-Cordoba B (2011)
Review: Authentication and traceability of foods from animal origin
by polymerase chain reaction-based capillary electrophoresis. Analytica
Chimica Acta 685:120-126.
SPSS (2006) IBM SPSS statistics version 130 for Windows IBM Corp.
Stănciuc (Sava), N, and Râpeanu, G (2010) Identification of adulterated
sheep and goat cheeses marketed in Romania by immunocromatographic
assay. Food Agr Immunol 21(2), 157-164
Turkish Food Codex (TFC) (2015) Prime Ministry General Directorate of
Legislation Development and Publication. https://www.resmigazete.
gov.tr/eskiler/2015/02/20150208-16.htm. [accesed 19 October 2023].
Turkish Food Codex (TFC) (2017) Prime Ministry General Directorate of
Legislation Development and Publication. https://www.resmigazete.
gov.tr/eskiler/2017/01/20170126M1-6.htm. [accesed 19 October
.
Tsakali E, Agkastra C, Koliaki C, Livanios D, Boutris G, Christopoulou
MI, Koulouris S, Koussissis S, Impe JFMV, Houhoula, D (2019) Milk
Adulteration: Detection of Bovine Milk in Caprine Dairy Products by
RT-PCR. J Food Res 8:52.
Tuncay RM (2023) Detection of adulteration of goat milk sold in the Turkish
market by real-time Polymerase Chain reaction. Ger J Vet Res
:18-23.
Tuncay RM, Sancak YC (2022) Comparison of PCR Methods for Determination
of Different Types of Milk Added to Goat Milk. Balıkesır
Health Sciences Journal 11:462-470.
Wen JG, Liu XJ, Wang ZM, Li TF, Wahlqvist ML (2020) Melamine-contaminated
milk formula and its impact on children. Asia Pac J Clinic
Nutr 25:697-705.
Zengin ZS, Kara R (2022) Investigation of milk origin in sheep and goat
cheese with Real-Time PCR. VetBio 7:179-184.