Site-directed mutagenesis in Brucella abortus S19 by overlap extension PCR-based procedure


Δημοσιευμένα: Jan 29, 2018
Λέξεις-κλειδιά:
Brucella abortus S19 Mutation Overlap extension polymerase chain reaction
B. NAYERI FASAEI
T. ZAHRAEI SALEHI
S. NASERLI
A. R SAEEDINIA
A. M. BEHROOZIKHAH
Περίληψη
ΔΕΝ ΥΠΑΡΧΕΙ ΠΕΡΙΛΗΨΗ.
Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Allen CA, Adams LG, Ficht TA (1998) Transposon-Derived Brucella abortus Rough Mutants Are Attenuated and Exhibit Reduced Intracellular Survival. Infection and Immunity 66(9):1008-1016.
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research 41(15):7429-7437.
Bryksin AV, Matsumura I (2010) Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. BioTechniques 48:463-465.
Chou IC, Olszewski NE, Cheng CP (2004) Generation of Random, In-Frame Mutations by TN1000-Mediated Mutagenesis. Journal of Genetics and Molecular Biology 2:137-142.
Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proceeding of the National Academy of Sciences of the United States of America 97(12):6640-6645.
Denamur E, Matic I (2006) Evolution of mutation rates in bacteria. Molecular Microbiology 60(4):820-827.
Higuchi R, Krummel B, Saiki RK (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Research 16(15):7351-7367.
Horton RM (1995) PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes. Molecular Biotechnology 3(2):93-99.
Horton RM, Cai ZL, Ho SN, Pease LR (1990) Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques 8(5):528-35.
Kahl-McDonagh MM, Ficht TA (2006) Evaluation of Protection Afforded by Brucella abortus and Brucella melitensis Unmarked Deletion Mutants Exhibiting Different Rates of Clearance in BALB/c Mice. Infection and Immunity 74(7):4048-4057.
Luo WG, Liu HZ, Lin WH, Kabir MH, Su Y (2013) Simultaneous splicing of multiple DNA fragments in one PCR reaction. Biological Procedures Online 15:9.
Miyoshi A, Rosinha GM, Camargo IL, Trant CM, Cardoso FC, Azevedo V,Oliveira SC (2007) The role of the vacB gene in the pathogenesis of Brucella abortus. Microbes and Infection 9(3):375-381.
Monreal D, Grillo MJ, Gonza´lez D (2003) Characterization of Brucella abortus O-Polysaccharide and Core Lipopolysaccharide Mutants and Demonstration that a Complete Core Is Required for Rough Vaccines To Be Efficient against Brucella abortus and Brucella ovis in the Mouse Model. Infection and Immunity 71(6):3261-3271.
Nikolich MP, Warren RL, Lindler LE, Izadjoo MJ, Hoover DL (2010) Attenuation of defined Brucella melitensis wboA mutants. Vaccine 28S: F12-F16.
Poester FP, Samartino LE, Santos RL (2013) Pathogenesis and pathobiology of brucellosis in livestock. Rev Sci Tech Off Int Epiz 32(1):105-115.
Soler-Lloréns P, Gil-Ramírez Y, Zabalza-Baranguá A, Iriarte M, CondeÁlvarez R, Zúñiga-Ripa A, San Román B (2014) Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice. Veterinary Research 45-72.
Ugalde JE, Comerci DJ, Leguizamón MS, Ugalde RA (2003) Evaluation of Brucella abortus Phosphoglucomutase (pgm) Mutant as a New Live Rough-Phenotype Vaccine. Infection and Immunity71(11):6264-6269.
Xavier MN, Paixão TA, Hartigh AB, Tsolis RM, Santos RL (2010) Pathogenesis of Brucella spp.. Veterinary Science Journal 4:109-118
Τα περισσότερο διαβασμένα άρθρα του ίδιου συγγραφέα(s)