The Serum Total and Lipid-Bound Sialic Acid with Hematological-Biochemical Parameter Levels in Dogs with Dirofilariosis

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Δημοσιευμένα: Απρ 29, 2022
DOI: https://doi.org/10.12681/jhvms.23827
Hasan Kesici
Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Biochemical, Van, Turkey
https://orcid.org/0000-0001-5464-0984
Yeter Değer
Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Biochemical, Van, Turkey
https://orcid.org/0000-0002-9023-0699
Bekir Oguz
Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Parasitology, Van, Turkey
https://orcid.org/0000-0001-8720-3940
Uğur Özdek
Van Yuzuncu Yil University, Vocational School of Health Services, Van, Turkey
https://orcid.org/0000-0002-0709-1545
Περίληψη
The present study was planned to investigate the potential changes in the levels of serum total sialic acid (TSA), lipid-bound sialic acid (LSA) and hematological-biochemical parameters in the dogs naturally infected by Dirofilaria immitis. The patient group included the 7 dogs clinically and parasitologically (Modified Knott and PCR) diagnosed with Dirofilaria immitis while 7 healthy dogs were assigned as the control group. The biochemical parameters, blood gas parameters and hematological parameters were tested in the blood samples taken properly according to the guidelines from all the study animals using modular auto-analyser, blood gas analyser and automated blood count devices, respectively. Serum TSA and LSA levels were measured spectrophotometrically using Sydow and Katapodis methods, respectively. Compared with the healthy group, the dogs diagnosed with dirofilariosis were found to have significantly increased levels of serum troponin, triglycerides, VLDL, LDL, BUN, urea and creatinine and enzymatic activities of CK-MB, ALT, AST, ALP and LDH, and statistically significantly decreased levels of cholesterol and HDL (p<0.05). The increases in levels of total protein and total bilirubin, and enzymatic activity of CK were found statistically insignificant (p>0.05). A significantly decreased level of RBC and a significantly increased level of WBC was determined in the dogs with dirofilariosis (p<0.05). The decreases in the levels of Hct and Hb were found statistically insignificant (p>0.05). The differential blood count test of the dogs with dirofilariosis indicated statistically significantly increased eosinophil count (p<0.05). The increases in the neutrophil and basophil counts, and the decreases in the lymphocyte and monocyte counts were detected to be statistically insignificant (p>0.05). In the dogs with dirofilariosis, the decreases in the levels of pCO2, HCO3 and BE as the venous blood gas parameters were detected to be statistically significant (p<0.05). The decreases in the levels of pH and pO2 were determined to be insignificant (p>0.05). The levels of serum TSA and LSA of the dogs with dirofilariosis were found statistically significantly higher than the healthy group (p<0.05). As a conclusion, statistically significant differences were identified between the dogs with dirofilariosis and healthy dogs in terms of sialic acid levels and certain biochemical, venous blood gas and hematological parameters. On the other hand, the present study is the first to investigate the serum sialic acid levels in the dogs with dirofilariosis.
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Αναφορές
Altıntaş A, Kurtdede A, Fidancı UR, Börkü MK (1989) Köpek gençlik hastalıgında (distemper) serum sialik asit ve protein düzeylerinin klinik önemi. Ankara Univ Vet Fak Derg 36 (1): 154-164.
Anonymous (2020) http: //vetlab.com/Canine%20and%20Feline%20Normal%20Values.pdf.
Aslan O, Yildirim A, Kanbur M, Altinordu S (2010) Detection of some biochemical and lipid peroxidation parameters in Dirofilaria immitis infected dogs. J Anim Vet Advan 9 (5): 954-957.
Atkins C (2005) Canine heartworm disease. In “Textbook of Veterinary Internal Medicine”, Ettinger SJ, Feldman EC, editors. 6 th ed., St. Louis. Elsevier 1118- 1136.
Atyha A H, Alani DAA (2017) Study the effects of naturally acquired canine dirofilariosis on some hematological and biochemical parameters. Iraqi J Vet Sci 41 (1): 104-108.
Balıkçı E (2005). Dirofilariosisli köpeklerin bazı klinik, hematolojik, biyokimyasal ve elektrokardiyogram bulguları. FÜ Sağlık Bil Derg 19 (1): 43-48.
Borthakur SK, Ali MA, Patra G (2011). Clinical, haematological and biochemical studies on Dirofilaria immitis in dog. J Vet Parasitol 25: 63-66.
Börkü MK, Kurtdede A, Azizoğlu D, Kilit M (1996) Dirofilaria immitis ile doğal enfekte köpeklerde thiacetarsamide sodium uygulamaları. Ankara Univ Vet Fak Derg 43 (2): 247-256.
Carretón E, Corbera JA, Juste MC, Morchón R, Simón F, Montoya-Alonso JA (2011) Dirofilaria immitis infection in dogs: cardiopulmonary biomarker levels. Vet Parasitol 176 (4): 313-316.
Carretón E, Grandi G, Morchón R, Simón F, Passeri B, Cantoni AM, Kramer L, Montoya-Alonso JA (2012) Myocardial damage in dogs affected by heartworm disease (Dirofilaria immitis): immunohistochemical study of cardiac myoglobin and troponin I in naturally infected dogs. Vet Parasitol 189 (2-4): 390-393.
Carretón E, Morchón R, González-Miguel J, Juste MC, Simón F, Montoya-Alonso JA (2013). Utility of cardiac biomarkers during adulticide treatment of heartworm disease (Dirofilaria immitis) in dogs. Vet Parasitol 197 (1-2): 244-250.
Carretón E, Morchón R, Simón F, Juste MC, Méndez JC, Montoya-Alonso JA (2014). Cardiopulmonary and inflammatory biomarkers in the assessment of the severity of canine dirofilariosis. Vet Parasitol 206 (1–2): 43-47.
Engen RL (1971). Serum sialic acid values in dogs with canine distemper. Amer J Vet Res 32 (5): 803-804.
Fırat İ, Gülçubuk A, Çetinkaya H (2005) İstanbul’da üç köpekte Dirofilaia İmmitis olgusu. İÜ Vet Fak Derg 31 (1): 187-193.
Gazyagci S, Dogru MT, Yagi BB (2011) Cardiac troponin I levels in dogs with dirofilariosis and without dirofilariosis. Asian J Anim Vet Adv 6: 738-743.
Gioia G, Lecová L, Genchi M, Ferri E, Genchi C, Mortarino M (2010) Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilaria immitis and Dirofilaria repens in canine peripheral blood. Vet Parasitol 172 (1-2): 160-163.
Goggin JM, Biller DS, Rost CM, DeBey BM, Ludlow CL (1997) Ultrasonographic identification of Dirofilaria immitis in the aorta and liver of a dog. JAVMA 210: 1635-1637.
Jacobs RM, Lums den JH, Vernau W (1992). Canine and feline reference values. In “Current Veterinary Therapy XI Small Animal Practice”. Philadelphia.
Karagül H, Altıntaş A, Fidancı UR, Sel T (2000) Klinik biyokimya. Ankara: Medisan Yayınevi.
Katopodis N, Hirshaut Y, Geller NL, Stock CC (1982) Lipid-associated sialic acid test for the detection of human cancer. Cancer Res 42 (12): 5270-5275.
Kına O (2009) Dirofilaria immitis’ li Köpeklerde Paraoksonaz Aktivitesi ve Lipid Profilinin Belirlenmesi. Mustafa Kemal Üniversitesi,Yüksek lisans tezi, Hatay.
Kitagawa H, Yasuda K, Sasaki Y (1993) Blood gas analysis in dogs pulmonary heartworm disease. J Vet Med Sci 55 (2): 275-280.
Loria AD Dattilo V, Santoro D, Guccione J, De Luca A, Ciaramella P, Pirozzi M, Laccino E (2020) Expression of serum exosomal mi RNA 122 and lipoprotein levels in dogs naturally infected by Leishmania infantum: A preliminary study. Animals 10 (1): 100.
Meral Y, Bakırel U, Çakıroğlu D (2007) Bir köpekte kalp kurdu hastalığının (Dirofilaria immitis) ekokardiyografik teşhisi. JIVS 3: 1-10.
Niwetpathomwat A, Assarasakorn S, Techangamsuwan A, Suvarnavibhaja S, Kaewthamasorn M (2006) Canine dirofilariosis and concurrent tick-borne transmitted diseases in Bangkok, Thailand. Comp Clin Pathol 15 (4): 249-253.
Niwetpathomwat A, Kaewthamasorn M, Tiawsirisup S, Techangamsuwan S, Suvarnvibhaja S (2007) A retrospective study of the clinical hematology and the serum biochemistry tests made on canine dirofilariosis cases in an animal hospital population in Bangkok, Thailand. Res Vet Sci 82 (3): 364-369.
Ok M, Öztürk AS, Er C (2010) Üç köpekte konjestif kalp yetmezliği. Eurasian J Vet Sci 26 (1): 57-62.
Pasca SA, Acatrinei D, Oprean OZ, Lazar M (2012) Vascular, hepatic and renal lesions by Dirofilaria immitis invasion in dogs. Arq Bras Med Vet Zootec 64: 841-846.
Ranjbar-Bahadori S, Mohri M, Helan JA, Jamshidi K, Kashefinejad M (2010) Clinico-Pathologic Evaluation of the Canine Heartworm Infestation. Res J Parasitol 5: 90-98.
Saritaş ZK, Akin F, Şahal M, Öcal N (2005) Open heart surgery applications in dogs suffering from natural infection of Dirofilaria immitis. Turk J Vet Anim Sci 29: 713-721.
Sevimli KF, Kozan E, Bülbül A, Birdane MF, Köse M, Sevimli A (2007) Dirofilaria immitis infection in dogs: unusually located and unusual findings. Parasitol Res 101:1487-1494.
Sribhen C, Kasemsant N, Kaewmokul S, Sribhen K (1999) Blood chemistry profile and cardiac troponin T concentration in Thai Stray dogs infected with heartworms. Kasetsart J Nat Sci 33: 251-257.
Sydow G, Wittmann W, Bender E, Starick E (1988) The sialic acid content of the serum of cattle infected with bovine leukosis virus. Arch Exp Vetmed 42: 194-197.
Şahal M, Özlem M, Tanyel B, Öcal N, Sel T (1997) Köpeklerdeki dirofilariosis olgularında kan, idrar ve abdominal sıvıda biyokimyasal değişiklikler. Ankara Üniv Vet Fak Derg 44: 267-276.
Tabrizi BA (2012) Evaluation of calcium, phosphorus and alkaline phosphatase in Dirofilaria immitis infection in dogs. World J Zool 7 (1): 79-82.
Taşkın E (2017) Ellajik Asitin Miyokart İnfarktüsü Oluşturulmuş Ratlarda Sialik Asit Düzeyleri ve Lipit Profili Üzerine Etkisi. Van Yüzüncü Yıl Üniversitesi, Yüksek lisans tezi, Van.
Taylor MA, Coop RL, Wall RL (2007) Veterinary parasitology. 3. West Sussex: Blackwell Publishing.
Thougaard AV, Hellmen E, Jensen AL (1998) Total serum sialic acid is a general disease marker rather than a specific tumour marker in dogs. J Vet Med A 45 (8): 471-479.
Thougaard AV, Hellmen E, Pedersen HD, Jensen AL (1999) Correlation between alpha 1-acid glikoprotein and total sialic acid in serum from dogs with tumours. J Vet Med A 46: 231-237.
Voyvoda H, Sekin S, Karaca M (1996) Therapeutic efficacy of ooramectin and levamisole in Dirofilaria immitis infected dogs and changes of some blood parameters. YYÜ Vet fak Derg 7 (1-2): 26-34.
Yılmaz B (2000). Fizyoloji. Ankara: Feryal Matbaacılık.
Yoon WK, Kim YW, Suh SL, Choi R, Lee SG, Hyun C (2017) Evaluation of cardiopulmonary and inflammatory markers in dogs with heartworm infection during treatment with the 2014 American Heartworm Society recommended treatment protocol. Parasit Vectors 10 ( 2): 535.