The biological potential of a product containing Pythium oligandrum against Uncinaria stenocephala (Railliet, 1884) larvae

PDF (English)
Δημοσιευμένα: Απρ 29, 2022
DOI: https://doi.org/10.12681/jhvms.25380
Iasmina Luca
Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
https://orcid.org/0000-0002-1153-6498
Mirela Imre
Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
https://orcid.org/0000-0003-3004-463X
Marius Stelian Ilie
Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
https://orcid.org/0000-0002-5484-4685
Ion Oprescu
Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
https://orcid.org/0000-0001-9968-1472
Gheorghe Dărăbuş
Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
https://orcid.org/0000-0002-4763-2617
Περίληψη

Pythium oligandrum is an oomycete commonly used in the biological control of plant and vegetable pests. In veterinary medicine is used to treat dermatophytosis produced by Microsporum canis. It acts as an antagonist, negatively influencing the development of other fungi. Through hydrolytic enzymes, such as kinase and cellulase, it destroys the cell wall and the internal cytoplasmic content. Many fungi are considered nematophagous. This potential has not been exploited for Pythium oligandrum, which is why in this study the potential larvicidal action against Uncinaria stenocephala larvae was investigated.

Ecosin® product, which contains P. oligandrum and other excipients, was used. A solution was prepared according to manufacturer. The Uncinaria stenocephala larvae were exposed to this substance and the action was investigated after 7 days. The results obtained showed 37.23% larval reduction.

Being an eco-friendly product, further studies are needed to improve the protocol for its use in cleaning spaces and surfaces in veterinary clinics, where various parasitic forms (larvae or eggs) are found.

Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Creative Commons License

Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.

Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.

Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.

Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc

Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.

Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Βιογραφικά Συγγραφέων
Iasmina Luca, Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
Department of Parasitology and Parasitic Diseases, PhD student
Mirela Imre, Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
Department of Parasitology and Parasitic Diseases, Lect
Marius Stelian Ilie, Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
Department of Parasitology and Parasitic Diseases, Assoc Prof
Ion Oprescu, Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
Department of Parasitology and Parasitic Diseases, Prof
Gheorghe Dărăbuş, Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timişoara
Department of Parasitology and Parasitic Diseases, Prof
Αναφορές
Benhamou N, le Floch G, Vallance J, Gerbore J, Grizard D, Rey P (2012) Pythium oligandrum: an example of opportunistic success. Microbiology 158 (11):2679-2694.
Bradshaw-Smith RP, Whalley WM, Craig GD (1991) Interactions between Pythium oligandrum and the fungal footrot pathogens of peas. Mycol Res 95:861-865.
Braga FR, Carvalho RO, Araujo JM, Silva AR, Araújo JV, Lima WS, Travela AO, Ferreira SR (2009) Predatory activity of the fungi Duddingtonia flagrans, Monacrosporium thaumasium, Monacrosporium sinense and Arthrobotrys robusta on Angiostrongylus vasorum first-stage larvae. J Helminthol 83 (4):303-308.
Braga FR, Carvalho RO, Silva AR, Araújo JV, Frassy LN, Lafisca A, Soares FEF (2014) Predatory capability of the nematophagous fungus Arthrobotrys robusta preserved in silica gel on infecting larvae of Haemonchus contortus. Trop Anim Health Prod 46 (3):571-574.
Costa TM, Tavares LBB, Oliveira D (2016) Fungi as a source of natural coumarins production. Appl Microbiol Biotechnol 100:6571-6584.
Darkin-Rattray SJ, Gurnett AM, Myers RW, Dulski PM, Crumley TM, Allocco JJ, Cannova C, Meinke PT, Steven L, Colletti SL, Bednarek MA, Singh SB, Goetz MA, Dombrowski AW, Polishook JD, Schmatz DM (1996) Apicidin: a novel antiprotozoal agent that inhibits parasite histone deacetylase. Proc Natl Acad USA Sci 93:13143-13147.
De Mello INK, Braga FR, Monteiro TS, Freitas LG, Araujo JM, Soares FE, Araújo JV (2014) Biological control of infective larvae of Ancylostoma spp. in beach sand. Rev Iberoam Micol 31:114-118.
Gabrielová A, Mencl K, Suchánek M, Klimeš R, Hubka V, Kolařík M (2018) The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses. Mycopathologia 183 (5):751‒764.
Gasser RB, Stewart LE, Speare R (1996) Genetic markers in ribosomal DNA for hookworm identification. Acta Trop 62:15-21.
Gerbore J, Vallance J, Yacoub A, Delmotte F, Grizard D, Regnault-Roger C, Rey P (2014) Characterization of Pythium oligandrum populations that colonise the rhizosphere of vines from the Bordeaux region. FEMS Microbiol Eco 90:153-167.
Horner NR, Grenville-Briggs LJ, van West P (2012) The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation. Fungal Biol 116:24-41.
Kirk PM, Cannon PF, David JC, Stalpers JA (2008) Dictionary of the fungi. 11th ed. Wallingford. CABI Publishing.
Kongsaeree P, Prabpai S, Sriubolmas N, Vongvein C, Wiyakrutta S (2003) Antimalarial dihydroisocoumarins produced by Geotrichum sp., an endophytic fungus of Crassocephalum crepidioides. J Nat Prod 66:709-711.
Loo CSN, Lam NSK, Yu D, Su XZ, Lu FF (2017) Artemisinin and its derivatives in treating protozoan infections beyond malaria. Pharmacol Res 117:192-217.
Maciel AS, Freitas LG, Campos AK, Lopes EA, Araújo JV (2010) The biological control of Ancylostoma spp. dog infective larvae by Duddingtonia flagrans in a soil microcosm. Vet Parasitol 173:262-270.
Martínez-Luis S, Cherigo L, Higginbotham S, Arnold E, Spadafora C, Ibañez A, Gerwick WH, Cubilla-Rios L (2011) Screening and evaluation of antiparasitic and in vitro anticancer activities of panamanian endophytic fungi. Int Microbiol 14 (2):95-102.
Metwaly AM, Ghoneim MM, Musa A (2015) Two new antileishmanial diketopiperazine alkaloids from the endophytic fungus Trichosporum sp. Der Pharma Chem 7 (11):322-327.
Nyongbela KD, Lannang AM, Ayimele GA, Ngemenya MN, Bickle Q, Efange S (2013) Isolation and identification of an antiparasitic triterpenoid estersaponin from the stem bark of Pittosporum mannii (Pittosporaceae). Asian Pac J Trop Dis 3 (5):389-392.
Picard K, Tirilly Y, Benhamou N (2000) Cytological effects of cellulases in the parasitism of Phytophthora parasitica by Pythium oligandrum. Appl Environ Microbiol 66:4305-4314.
Saumell CA, Fernández AS, Echevarria F, Gonçalves I, Iglesias L, Sagües MF, Rodríguez EM (2016) Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi. J Helminthol 90:706-711.
Silva LMC, Miranda RRC, Santos HA, Rabelo ÉML (2006) Differential diagnosis of dog hookworms based on PCR-RFLP from the ITS region of their rDNA. Vet Parasitol 140:373-377.
Soares FE, Braga FR, de Araújo JV, Lima Wdos S, de Queiroz JH (2015) The nematophagous fungus Monacrosporium thaumasium and its nematicidal activity on Angiostrongylus vasorum. Rev Iberoam Micol 32 (1):51-53.
Takenaka S, Tamagake H (2009) Foliar spray of a cell wall protein fraction from the biocontrol agent Pythium oligandrum induces defence-related genes and increases resistance against Cercospora leaf spot in sugar beet. J Gen Plant Pathol 75:340.