A novel approach to sperm selection: Nanoparticle-based purification improves quality of Angora cryopreserved buck’s semen

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Δημοσιευμένα: Jan 21, 2023
DOI: https://doi.org/10.12681/jhvms.28206
H Alemdar
Ankara University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination
MB Tırpan
Ankara University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination
https://orcid.org/0000-0001-8782-1108
Περίληψη

The Aim: The aim of the study was to investigate the applicability of an up-to-date method, sperm selection with nanoparticles at different temperatures (37ºC, 21ºC, and 4ºC), on Angora buck semen. Material and Methods: Semen samples were collected from 3 Angora bucks with 7 repetitions and each sample was diluted and divided into 4 groups. Nanopurification procedure was applied by using a nanoparticle dose of 10 μg/ml to the three groups at 37ºC, 21ºC, and 4ºC while in the control group samples were cryopreserved without nanopurificiation treatment. Post-thaw motility and morphology parameters of the sperm samples were analyzed by using computer-aided sperm analysis (CASA) and fluorescein microscopy.

Results: After nanopurification at 37ºC total motility, linearity (LIN) (P<0.05), curvilinear velocity (VCL), straight-line velocity (VSL), and acrosome integrity parameters (P<0.001) were higher than the control group while the semen samples nanopurified at 21ºC were superior to the control group in terms of LIN, straightness (STR) (P<0.05), VCL, VSL, average path velocity (VAP), wobble and acrosome integrity (P<0.001). There was no significant difference between the control and the 4ºC group.

Conclusion: In conclusion, nanopurification procedure on buck semen was useful in terms of parameters that affect fertility and the method can be performed at 21ºC, as well as 37ºC, to adapt the procedure to field conditions;

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Λήψεις
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Βιογραφικά Συγγραφέων
H Alemdar, Ankara University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination

Department of Reproduction and Artificial Insemination, PhD

MB Tırpan, Ankara University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination

Department of Reproduction and Artificial Insemination, associate professor

Αναφορές
Aitken RJ. Baker MA, Nixon B (2015) Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress? Asian J. Androl. 17: 633–639.
Al-Essawe E, Johannisson A, Wulf M, Aurich C, Morrell J (2018) Improved cryosurvival of stallion spermatozoa after colloid centrifugation is independent of the addition of seminal plasma, Cryobiology. 81: 145–152.
Arias ME, Andara K, Briones E, Felmer R (2017) Bovine sperm separation by Swim-up and density gradients (Percoll and BoviPure): Effect on sperm quality, function and gene expression, Reprod. Biol. 17: 126–132.
Barbas JP, Leahy T, Horta AE, Garcia-Herreros M (2018). Sperm kinematics and subpopulational responses during the cryopreservation process in caprine ejaculates. Cryobiology 82: 137-147.
Baska KM., Manandhar G, Feng D, Agca Y, Tengowski MW, Sutovsky M, Yi YJ, Sutovsky P (2008) Mechanism of extracellular ubiquitination in the mammalian epididymis, J. Cell. Physiol. 215: 684–696.
Brinsko SP, Blanchard TL, Rigby SL, Love CC, Varner DD (2003) Effects of dead spermatozoa on motion characteristics and membrane integrity of live spermatozoa in fresh and cooled-stored equine semen, Theriogenology. 59: 735–742.
Clemente-Associates Inc., Magnetic nanoparticles: Fertility improvement in subfertile animals and humans., (2016).
Crespo-Félez I, Castañeda-Sampedro A, Sánchez DI, Fernández-Alegre E, Álvarez-Rodríguez M, Domínguez JC, Morrell JM, Martínez-Pastor F (2017) Effect of Single Layer Centrifugation Porcicoll (70%, 80% and 90%) or supplementation with reduced glutathione, seminal plasma and bovine serum albumin on frozen-thawed boar sperm, Anim. Reprod. Sci. 187: 167–173.
Durfey JM, Swisteck CL, Wei S.E, Clemente T, Ryan H, Willard PL, Feugang ST (2017) Beneficial effects of semen purification with magnetic nanoparticles, J. Miss. Acad. Sci. 62: 163–166.
Feugang JM (2017) Novel agents for sperm purification, sorting, and imaging, Mol. Reprod. Dev. 84: 832–841.
Feugang JM, Rhoads CE, Mustapha PA, Tardif S, Parrish JJ, Willard ST, Ryan PL (2019) Treatment of boar sperm with nanoparticles for improved fertility, Theriogenology. 137: 75–81.
Figuerola A, di Corato R, Manna L, Pellegrino T (2010) From iron oxide nanoparticles towards advanced iron-based inorganic materials designed for biomedical applications, Pharmacol. Res. 62: 126–143.
Fruend M, Wiederman J (1966) Factors affecting the dilution, freezing, and storage of human semen, Obstet. Gynecol. Surv. 21: 655–656.
Galarza DA, López-Sebastián A, Woelders H, Blesbois E, Santiago-Moreno J (2018) Sephadex filtration as successful alternative to density-gradient centrifugation procedures for ram sperm selection with improved kinetics, Anim. Reprod. Sci. 192: 261–270.
Gallon F, Marchetti C, Jouy N, Marchetti P (2006) The functionality of mitochondria differentiates human spermatozoa with high and low fertilizing capability, Fertil. Steril. 86: 1526–1530.
Garner DL, Thomas CA, Joerg HW, DeJarnette JM, Marshall CE (1997) Fluorometric Assessments of Mitochondrial Function and Viability in Cryopreserved Bovine Spermatozoa1, Biol. Reprod. 57: 1401–1406.
Gravance CG, Garner DL, Baumber J, Ball BA (2000) Assessment of equine sperm mitochondrial function using JC-1, Theriogenology. 53: 1691–1703.
Gravance CG, Garner DL, Miller MG, Berger T (2000) Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function, Reprod. Toxicol. 15: 5–10.
Grudzinskas JG, Yovich JL (1995) Sperm structure and function, in: Gametes - The Spermatozoon
Grunewald S, Paasch U, Said TM, Rasch M, Agarwal A, Glander HJ (2006) Magnetic-activated cell sorting before cryopreservation preserves mitochondrial integrity in human spermatozoa, Cell Tissue Bank. 7: 99–104.
Grunewald S, Said TM, Paasch U, Glander HJ, Agarwal A (2008) Relationship between sperm apoptosis signalling and oocyte penetration capacity, Int J Androl. 31: 325–330.
Haenlein GFW (1995) Angora goat and mohair production, Small Rum Res. 16 94.
Holt C, Holt WV, Moore HDM, Reed HCB, Curnock RM (1997). Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations; Results of two fertility trials. Androl 18(3): 312-323.
Husna AU, Ejaz R, Qadeer S, Azam A, Rakha BA, Ansari MS, Shahzad Q, Javed M, Vazquez-Levin MH, Akhter S (2016) A comparative analysis of sperm selection procedures prior to cryopreservation for Nili-Ravi buffalo bull (Bubalus bubalis) semen-: Assessment of its impact on post-thaw sperm functional quality, Anim. Reprod. Sci. 174: 29–36.
Ibrahim NM, Gilbert GR. Loseth KJ, Crabo BG (2000) Correlation between clusterin-positive spermatozoa determined by flow cytometry in bull semen and fertility, J Androl. 21: 887–894.
Inanç ME, Tekin K, Olğaç KT, Tirpan MB, Alemdar H, Cil B, Kaya U, Stelletta C, Daskin A (2017) Effects of region and invidualism traits on sperm freezeability of Angora Goats, Vet.Hekim.Der.Derg. 88: 31–39.
Inanç ME, Uysal O, Gürcan IS (2014) Farklı Irklardan Tekelerde Başlıca Spermatolojik Parametreler, MAKÜ Sag Bil Enst Derg. 2: 13-19–19.
Leboeuf B, Restall B, Salamon S (2000) Production and storage of goat semen for artificial insemination, Anim. Reprod. Sci. 62: 113–141.
Lee TH, Liu CH, Shih YT, Tsao HM, Huang CC, Chen HH, Lee MS (2010) Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility, Hum Reprod. 25: 839–846.
Li L, Ning Y, Yin H, Sun Z (2003). The application of computer assisted sperm analysis on in vitro fertilization, Sichuan Da Xue Xue Bao Yi Xue Ban 34(1): 112-114.
Li Y, Liu C, Feng X, Xu Y, Liu BF (2014) Ultrafast microfluidic mixer for tracking the early folding kinetics of human telomere G-quadruplex, Anal Chem. 86: 4333–4339.
Liu BY, Clarke GN, Baker HWG (1991). Relationship between sperm motility assessed with the Hamilton-Thorn motility analyzer and fertilization rates in vitro., J Androl 12: 231-239.
Lu JC, Huang YF, Lü NQ (2014). Computer-aided sperm analysis: past, present and future. Andrologia 46(4):329-338.
Martinez-Alborcia MJ, Valverde A, Parrilla I, Vazquez JM, Martinez EA, Roca J (2012) Detrimental effects of non-functional spermatozoa on the freezability of functional spermatozoa from Boar Ejaculate, PLoS ONE. 7
Mohammed L, Gomaa HG, Ragab D, Zhu J (2017) Magnetic nanoparticles for environmental and biomedical applications: A review, Particuology. 30: 1–14.
Morrell J, Colloids (2016) Applications in Sperm Preparation for Assisted Reproduction, in Advances in Colloid Science
Mortimer D (2000) Sperm preparation methods, J Andro. 21: 357–366.
Nadalini M, Tarozzi N, di Santo M, Borini A (2014) Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: Is the new approach better than the traditional one?, J Assist Reprod Genet. 31: 1045–1051.
Nagy S, Jansen J, Topper EK, Gadella BM (2003) A Triple-Stain Flow Cytometric Method to Assess Plasma- and Acrosome-Membrane Integrity of Cryopreserved Bovine Sperm Immediately after Thawing in Presence of Egg-Yolk Particles1, Biol. Reprod. 68: 1828–1835.
Niederberger C (2016) Re: Separation Efficiency of a Microfluidic Sperm Sorter to Minimize Sperm DNA Damage, J Urol. 196 859.
Noguchi M, Yoshioka K, Hikono H, Iwagami G, Suzuki C, Kikuchi K (2014) Centrifugation on Percoll density gradient enhances motility, membrane integrity and in vitro fertilizing ability of frozen-thawed boar sperm, Zygote. 23: 68–75.
Nongbua T, Johannisson A, Edman A, Morrell JM (2017) Effects of single layer centrifugation (SLC) on bull spermatozoa prior to freezing on post-thaw semen characteristics, Reprod Domest Anim. 52: 596–602.
Odhiambo JF, DeJarnette J, Geary TW, Kennedy CE, Suarez SS, Sutovsky M, Sutovsky P (2014) Increased Conception Rates in Beef Cattle Inseminated with Nanopurified Bull Semen1, Biol. Reprod.. 91
Olson GE, Winfrey VP, NagDas SK, Melner MH (2004) Region-specific expression, and secretion of the fibrinogen-related protein, fgl2, by epithelial cells of the hamster epididymis and its role in disposal of defective spermatozoa, J Biol Chem. 279: 51266–51274.
Parinaud J, Vieitez G, Moutaffian H, Richoilley G, Milhet P (1996). Relationships between motility parameters, morphology and acrosomal status of human spermatozoa, Hum. Reprod. 11: 1240 – 1243
Roach J, Schnobrich M, Ellerbrock R, Feijo L, Bradecamp E, Alvarenga MA, Kline K, Canisso I (2016) Paper Comparison of cushioned centrifugation and Sperm Filter filtration on longevity and morphology of cooled-stored equine semen, Vet Rec.
Roca J, Parrilla I, Gil MA, Cuello C, Martinez EA, Rodriguez-Martinez H (2016) Non-viable sperm in the ejaculate: Lethal escorts for contemporary viable sperm, Anim. Reprod. Sci.. 169: 24–31.
Rodríguez-Martínez H (2007) State of the art in farm animal sperm evaluation, Reprod Fertil Dev. 19: 91–101.
Said TM, Agarwal A, Grunewald S, Rasch M, Glander HJ, Paasch U (2006) Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation, Reprod Biomed Online. 13: 336–339.
Said TM, Agarwal A, Zborowski M, Grunewald S, Glander HJ, Paasch U (2008) Utility of magnetic cell separation as a molecular sperm preparation technique, J Andro. 29: 134–142.
Said TM, Grunewald S, Paasch U, Rasch M, Agarwal A, Glander HJ (2005) Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates, Fertil Steril. 83: 1442–1446.
Schaäfer S, Holzmann A (2000) The use of transmigration and SpermacTMstain to evaluate epididymal cat spermatozoa, Anim. Reprod. Sci.. 59: 201–211.
Shannon P, Curson B (1972) Toxic Effect and Action of Dead Sperm on Diluted Bovine Semen, J Dairy Sci. 55: 614–620.
Shengfa F Liao JMF (2014) Lectin-Functionalized Magnetic Iron Oxide Nanoparticles for Reproductive Improvement, Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics and Stem Cell. 03
Suarez SS, Wu M (2017) Microfluidic devices for the study of sperm migration, Mol Hum Reprod. 23: 227–234.
Sutovsky P, Kennedy CE (2013) Biomarker-based nanotechnology for the improvement of reproductive performance in beef and dairy cattle, Ind Biotechnol. 9: 24–30.
Sutovsky P, Lovercamp K (2010) Molecular markers of sperm quality., Soc Reprod Fertil Suppl. 67: 247–256.
Tekin K, Daşkın A (2019) Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation, Cryobiology. 89 60–67.
Tirpan MB, Tekin N (2015) Effects of boron (sodium pentaborate), added instead of Tris components, on freezing and post-thaw quality of Angora buck semen, Ankara Univ Vet Fak Derg. 62: 295–302.
Urbano-Bojorge AL, Casanova-Carvajal O, Félix-González N, Fernández L, Madurga R, Sánchez-Cabezas S, Aznar E, Ramos M, Serrano-Olmedo JJ (2018) Influence of medium viscosity and intracellular environment on the magnetization of superparamagnetic nanoparticles in silk fibroin solutions and 3T3 mouse fibroblast cell cultures, Nanotechnology. 29: 1–13.
Volpe S, Leoci R, Aiudi G, Lacalandra GM (2009) Relationship between motility and mitochondrial functional status in canine spermatozoa, Reprod Domest Anim. 44: 275–278.
Watson PF (2000) The causes of reduced fertility with cryopreserved semen, Anim Reprod Sci. 60–61: 481–492.