Characterization of Bovine Bone Marrow Derived Mesenchymal Stem Cells and Immunostaining of Differentiated Neurospheres

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Δημοσιευμένα: Jul 4, 2023
Εκδόσεις:
2023-07-04 (1)
DOI: https://doi.org/10.12681/jhvms.28390
T Dikmen
Afyon Kocatepe University, Faculty of Veterinary Medicine
https://orcid.org/0000-0003-4470-7465
Ö Özden Akkaya
Department of Histology and Embryology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
S Nawaz
Department of Histology and Embryology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
M Erdoğan
Department of Veterinary Biology and Genetics, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
Περίληψη

Stem cell differentiation has been a key element for the therapeutical applications for various cases. The novel anti-inflammatory and angiogenic findings and potential applications for both autologous and allogenic studies have emphasized about the usage of mesenchymal stem cells in the clinical trials. The neurosphere differentiation studies and their role for peripheral nerve injuries have gained momentum. Following study emphasized on the isolation of stem cells from bovine bone marrow, their characterization by PCR and their differentiation into mesodermal lineages and into neurospheres under specific induction media. The generated neurospheres were durther investigated for their potential expressions of neuroprogenitor markers Sox2, Nestin and neurofilament protein β-III Tubulin by immunofluorescence staining. The results of following study highlight the use of bone marrow derived mesenchymal stem cells for translational research and regenerative therapy.

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Αναφορές
Bez A, Corsini E, Curti D, Biggiogera M, Colombo A, Nicosia RF, Pagano SF, Parati EA (2003) Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization. Brain Res, 993, 18-29.
Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T (2006) Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis. Biotechnol Bioeng. 93.6, 1152-1163.
Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T (2005) Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells. Cell Tissue Res. 319, 243-253.
Brazel CY, Limke TL, Osborne JK, Miura T, Cai J, Pevny L, Rao MS (2005) Sox2 expression defines a heterogeneous population of neurosphere‐forming cells in the adult murine brain. Aging cell, 2005, 4, 197-207.
Bylund M, Andersson E, Novitch BG, Muhr J (2003) Vertebrate neurogenesis is counteracted by Sox1–3 activity. Nat Neurosci. 6, 1162-1168.
Cortes Y, Ojeda M, Araya D, Dueñas F, Fernández MS, Peralta OA (2013) Isolation and multilineage differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res. 9, 133.
D'Amour KA, Gage FH (2003) Genetic and functional differences between multipotent neural and pluripotent embryonic stem cells. Proc Natl Acad Sci U S A. 100.suppl 1: 11866-11872.
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS, Deans RJ, Keating A, Prockop DJ, Horwitz EM (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 8, 315-317.
Dueñas F, Becerra V, Cortes Y, Vidal S, Sáenz L, Palomino J, De Ios Reyes M, Peralta OA (2014) Hepatogenic and neurogenic differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res. 10, 154.
Friedenstein A, Piatetzky-Shapiro I, Petrakova K (1966) Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol. 16, 381-390.
Friedenstein A J, Chailakhyan RK, Latsinik NV, Panasyuk AF, Keiliss-Borok IV (1974) Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues: cloning in vitro and retransplantation in vivo. Transplantation. 17, 331-340.
Gao Y, Zhu Z, Zhao Y, Hua J, Ma Y, Guan W (2014) Multilineage potential research of bovine amniotic fluid mesenchymal stem cells. Int J Mol Sci. 15, 3698-3710.
Graham V, Khudyakov J, Ellis P, Pevny, L (2003) SOX2 functions to maintain neural progenitor identity. Neuron, 39; 749-765.
Harding J, Roberts RM, Mirochnitchenko O (2013) Large animal models for stem cell therapy. Stem Cell Res Ther, 4, 1-9.
Hill ABT., Bressan FF, Murphy BD, Garcia JM (2019) Applications of mesenchymal stem cell technology in bovine species. Stem Cell Res Ther, 10, 1-13.
Huss R, Hoy C, Deeg H (1995) Contact-and growth factor-dependent survival in a canine marrow-derived stromal cell line. Blood. 85, 2414–2421.
Ireland JJ, Roberts RM, Palmer GH, Bauman DE, Baze, FW (2008) A commentary on domestic animals as dual-purpose models that benefit agricultural and biomedical research. J Anim Sci. 86, 2797-2805.
Jaiswal N, Haynesworth SE, Caplan AI, Bruder, S (1997) Osteogenic differentiation of purified, culture‐expanded human mesenchymal stem cells in vitro. J Cell Biochem. 64, 295-312.
Kalendar R, David L, Schulman HA (2009) "FastPCR software for PCR primer and probe design and repeat search. Genes, Genomes and Genomics 3, 1-14.
Lee SH, Cha SH, Kim CL, Lillehoj HS, Song JY, Lee KW (2015) Enhanced adipogenic differentiation of bovine bone marrow-derived mesenchymal stem cells. J Appl Anim Res. 43, 15-21.
Lillie R, Ashburn LL (1943) Supersaturated solutions of fat stains in dilute isopropanol for demonstration of acute fatty degeneration not shown by Herxheimer’s technique. Arch Pathol. 36, 432-440.
Martin DR, Cox NR, Hathcock TL, Niemeyer GP, Baker HJ (2002) Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow. Exp Hematol, 30, 879-886.
P Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science. 284, 143-147.
Reiprich S, Wegner M (2014) Sox2: a multitasking networker. Neurogenesis, 2014, 1.1: e962391.
Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 255.5052: 1707-1710.
Ringe J, Kaps C, Schmitt B, Büscher K, Bartel J, Smolian H,Schultz O, Burmester GR, Haupl T, Sittinger M (2002) Porcine mesenchymal stem cells. Cell Tissue Res. 307, 321-327.
Roth JA, Tuggle CK (2015): Livestock models in translational medicine. ILAR J. 56, 1-6.
Sasaki R, Aoki S, Yamato M, Uchiyama H, Wada K, Ogiuchi H, Okano T, Ando T (2010) A protocol for immunofluorescence staining of floating neurospheres. Neuroscience Lett. 479, 126-127.
Strutt B, Khalil W, Killinger D (1996) Growth and differentiation of human adipose stromal cells in culture. Methods Mol Med. 2, 41-51.