First report of detection of Canine Parvovirus type 2 in naturally infected domestic cats in Egypt by duplex PCR for simultaneous detection of Canine Parvovirus type 2 and Feline Panleukopenia virus

PDF (English)
Δημοσιευμένα: Oct 16, 2023
DOI: https://doi.org/10.12681/jhvms.28838
AF Magouz
Department of virology Fac of Vet Med Kafrelsheikh University ,Egypt
https://orcid.org/0000-0001-8870-9652
I Elkon
Department of Gynecology Fac of Vet Med Kafrelsheikh University ,Egypt
https://orcid.org/0000-0002-2980-2360
E Khaled
Department of virology Fac of Vet Med Kafrelsheikh University ,Egypt
N Alkhalefa
Department of virology Fac of Vet Med Kafrelsheikh University ,Egypt
Περίληψη

Feline Panleukopenia virus (FPLV) and Canine Parvovirus type 2 (CPV) cause fatal gastroenteritis in cats and dogs. In this study we developed a duplex polymerase chain reaction (dPCR) assay for the concurrent detection of FPLV and CPV-2 in a single PCR tube. Two primers were used based on nucleic acid conserved regions of the two viruses which specifically amplify 237 bp of the VP2 gene of FPLV and 583 bp of the VP2 gene of CPV 2.Sensitivity and Specificity of the dPCR were evaluated. A total of 30 rectal/fecal swabs were collected from domestic cats in Kafrelsheikh province, Egypt and were tested for FPLV and CPV-2 viruses using the dPCR assay. The results revealed that this dPCR assay was sensitive, as it could detect a minimum of 1 × 105 copies of genomic DNA of the two viruses. The dPCR assay was highly specific as there was no amplification of nucleic acid of other feline and canine pathogens. The positive ratio was 83.3% (25/30) for FPLV and 16.6% (5/30) for CPV respectively. Further analyses of CPV samples by Restriction Fragment Length Polymorphism (RFLP) revealed that they are classified as CPV 2a/2b variants. This study reports the first detection of CPV 2a/2b from symptomatic cats in Egypt using dPCR assay that can detect FPLV and CPV in a single tube reaction. 

Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Creative Commons License

Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.

Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.

Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.

Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc

Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.

Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Abd-Eldaim M, Beall MJ and Kennedy MA, 2009. Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus. Vet Ther 10:1-6.
------------------------------------------------------------------------------------------------------------------------
Ahmed N, Riaz A, Zubair Z, et al., 2018. Molecular analysis of partial VP-2 gene amplified from rectal swab samples of diarrheic dogs in Pakistan confirms the circulation of canine parvovirus genetic variant CPV-2a and detects sequences of feline panleukopenia virus (FPV). Virol J 15: 15:45.
------------------------------------------------------------------------------------------------------------------------
Awad RA, Khalil WKB and Attallah AG, 2018. Epidemiology and diagnosis of feline panleukopenia virus in Egypt: Clinical and molecular diagnosis in cats. Vet World 11:578-584.
------------------------------------------------------------------------------------------------------------------------
Awad RA, Khalil WKB and Attallah AG, 2018. Feline panleukopenia viral infection in cats: Application of some molecular methods used for its diagnosis. J Genet Eng Biotechnol 16:491-497.
------------------------------------------------------------------------------------------------------------------------
Balboni A, Bassi F, De Arcangeli S, et al., 2018. Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats. BMC Vet Res 14:41.
------------------------------------------------------------------------------------------------------------------------
Barrs VR, 2019. Feline Panleukopenia: A Re-emergent Disease. Vet Clin North Am Small Anim Pract 49:651-670.
------------------------------------------------------------------------------------------------------------------------
Battilani M, Balboni A, Ustulin M, et al., 2011. Genetic complexity and multiple infections with more Parvovirus species in naturally infected cats. Vet Res 2:42-43.
------------------------------------------------------------------------------------------------------------------------
Brindhalakshmi B, Mukhopadhyay HK, Antony PX, et al., 2016. Isolation and molecular characterization of canine and feline parvovirus strains - an updated review. J Dairy Vet Anim Res 3:164‒169.
------------------------------------------------------------------------------------------------------------------------
Buonavoglia C, Martella V, Pratelli A, et al., 2001. Evidence for evolution of canine parvovirus type-2 in Italy. J Gen Virol 82:1555–1560.
------------------------------------------------------------------------------------------------------------------------
Capozza P, Buonavoglia VC, et al., 2021. Emerging Parvoviruses in Domestic Cats. Viruses 13:1077.
------------------------------------------------------------------------------------------------------------------------
Chung HC, Kim SJ, Nguyen VG, et al., 2020. New genotype classification and molecular characterization of canine and feline parvoviruses. J Vet Sci 21:e43.
------------------------------------------------------------------------------------------------------------------------
Decaro N, Buonavoglia D, Desario C, et al., 2010. Characterisation of canine parvovirus strains isolated from cats with feline panleukopenia. Res Vet Sci 89:275-8.
------------------------------------------------------------------------------------------------------------------------
Ikeda Y, Mochizuki M, Naito R, et al., 2000. Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats. Virol J 278:13-19.
------------------------------------------------------------------------------------------------------------------------
Kasem S, El-Abasy M, El-Bagory G, et al., 2014. Molecular characterization and Phylogenetic analysis of avian influenza virus circulating in Kafrelsheikh Governorate. Egypt Glob Vet 12: 532–540
------------------------------------------------------------------------------------------------------------------------
Kim SG, Kang MH and Park HM, 2017. Comparative study of two point-of-care enzyme linked immunosorbent assays for the detection of antibodies against canine parvovirus and canine distemper virus. Pak Vet J, 37: 405-410.
------------------------------------------------------------------------------------------------------------------------
Liu J, Wu Q and Qiu H, 2017. Effect of kuqin compound total polysaccharides on CD4+, CD8+ T cells and cytokines of blood in dogs infected by canine parvovirus. Pak Vet J, 37: 305-310.
------------------------------------------------------------------------------------------------------------------------
Mochizuki M, Horiuchi M, Hiragi H, et al., 1996. Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia. J ClinMicrobiol 34:2101-2106
------------------------------------------------------------------------------------------------------------------------
Mukhopadhyay HK, Nookala M, Thangamani NR, et al., 2017. Molecular characterisation of parvoviruses from domestic cats reveals emergence of newer variants in India. J Feline Med Surg 19:846-852.
------------------------------------------------------------------------------------------------------------------------
Muz D, Oğuzoğlu TC, Timurkan MO, et al., 2012. Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey. Virus Genes 44:301-8.
------------------------------------------------------------------------------------------------------------------------
Nakamura K, Sakamoto M, Ikeda Y, et al., 2001. Pathogenic potential of canine parvovirus types 2a and 2c in domestic cats. ClinDiagn Lab Immunol 8:663-668.
------------------------------------------------------------------------------------------------------------------------
Neuerer F, Horlacher K, Truyen U, et al., 2008. Comparison of different in-house test systems to detect parvovirus in faeces of cats. J Feline Med Surg 10:247-51.
------------------------------------------------------------------------------------------------------------------------
Niu J, Yi S, Hu G, et al., 2018. Prevalence and molecular characterization of parvovirus in domestic kittens from Northeast China during 2016–2017. Jpn J Vet Res 66:145–155
------------------------------------------------------------------------------------------------------------------------
Ohshima T and Mochizuki M, 2009. Evidence for recombination between feline panleukopenia virus and canine parvovirus type 2. J Vet Med Sci 71:403-8.
------------------------------------------------------------------------------------------------------------------------
Steinel A, Munson L, van Vuuren M, et al., 2000. Genetic characterization of feline parvovirus sequences from various carnivores. J. Gen. Virol 81: 345-350
------------------------------------------------------------------------------------------------------------------------
Stuetzer B and Hartmann K, 2014. Feline parvovirus infection and associated diseases. Vet J 201:150-5.
------------------------------------------------------------------------------------------------------------------------
Sun Y, Cheng Y and Lin P, 2019. Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis. BMC Vet Res 10; 15:141.
------------------------------------------------------------------------------------------------------------------------
Truyen U, Evermann JF, Vieler E, et al., 1996. Evolution of canine parvovirus involved loss and gain of feline host range. Virol J 215:186-9.
------------------------------------------------------------------------------------------------------------------------
Yi S, Niu J, Dong H, et al., 2018. Rapid detection of feline calicivirus and feline herpesvirus by duplex nested RT-PCR. Pak Vet J 38: 347-352.
------------------------------------------------------------------------------------------------------------------------
Zhang Q, Niu J, Yi S, et al., 2019. Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus. Arch Virol 164: 2761–2768.
------------------------------------------------------------------------------------------------------------------------
Zobba R, Visco S, Sotgiu F, et al., 2021. Molecular survey of parvovirus, astrovirus, coronavirus, and calicivirus in symptomatic dogs. Vet Res Commun 45:31-40.