Effect of sub-inhibitory concentrations of antibiotic on the production and N-acetylglucosamine scale of methicillin- resistant Staphylococcus aureus biofilm

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Δημοσιευμένα: Apr 11, 2023
Εκδόσεις:
2023-04-11 (1)
DOI: https://doi.org/10.12681/jhvms.29576
N Moori Bakhtiari
https://orcid.org/0000-0002-1884-088X
M Ezzati Givi
S Goudarzi
Περίληψη

The ability of Staphylococcus spp. to produce biofilm is one of the virulence factors that facilitate the adhesion and colonization on a different surface. In this study, the effects of sub minimum inhibitory concentration (sub-MIC) of some antibiotics were evaluated on induction of the biofilm producing ability and free N-acetylglucoseamine scale in 29 isolates of methicillin resistance staphylococcus aureus. To this end, the antibiogram and biofilm producing functions of the studied isolates were assessed by Kirby-Bauer and tissue culture plate method, respectively. Vancomycin, trimethoprim-sulfamethoxazole and clindamycin were used in antibiogram. The free N-acetylglucoseamine scale in the inducted biofilm after treatment with antibiotic was evaluated by TLC method. Based on the attained results, all the isolates were susceptible to vancomycin and were capable of producing biofilm in weak (40%), moderate (56.6%) and strong (3.33%) levels. Also, biofilm production was induced in 36.66% of isolates (11/30) from moderate to strong level by sub-MIC vancomycin. An invisible change in free N-acetyl glucoseamine scale was demonstrated in the exopolysaccharide (EPS) structure of the studied isolates biofilm.

By comparing of results and literature reviews, free N-acetyl glucoseamine scale in all studied strains was lower than 5µg in before and after inducted biofilm or maybe is not exist. Certainly, for studied of structural N-acetyl glucoseamine scale, using more exact methods of extraction and measurement are need.

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Αναφορές
Adugna F, Pal M, Girmay G. (2018) Prevalence and Antibiogram assessment of Staphylococcus aureus in beef at municipal abattoir and butcher shops in Addis Ababa, Ethiopia. BioMed Res Int 2: 237-241.
Águila-Arcos S, Álvarez-Rodríguez I, Garaiyurrebaso O, et al. (2017) Biofilm-forming clinical Staphylococcus isolates harbor horizontal transfer and antibiotic resistance genes. Front Microbiol 8: 201-9.
Arciola CR, Campoccia D, Baldassarri L, et al. (2006) Detection of biofilm formation in Staphylococcus epidermidis from implant infections. Comparison of a PCR method that recognizes the presence of ica genes with two classic phenotypic methods. J Biomed Mater Res 76: 425-30.
Arciola CR, Campoccia D, Speziale P, et al. (2012) Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm resistant materials. Biomaterials 33: 5967–5982.
Atshan SS, Nor Shamsudin MN, Sekawi Z, et al. (2012) Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus. J BioMed Biotech 1-10. Article ID 976972.
Bales PM, Renke EM, May SL, et al. (2013) Purification and characterization of biofilm-associated EPS exopolysaccharides from ESKAPE organisms and other pathogens. Plos One 8 : 67950.
Beenkeen KE, Dunman PM, McAleese F, et al. (2004) Global gene expression in Staphylococcus aureus biofilms. J Bacteriol 186: 4665–4684.
Bock SN, Lee RE, Fisher B, et al. (1990) A prospective randomized trial evaluating prophylactic antibiotics to prevent triple-lumen catheter-related sepsis in patients treated with immunotherapy. J Clin Oncol 8: 161-9.
Boles BR, Horswill AR (2012) Swimming cells promote a dynamic environment within biofilms. National Academy of Sciences 109: 12848-12849.
Cargill JS, Upton M. (2009) Low concentrations of vancomycin stimulate biofilm formation in some clinical isolates of staphylococcus epidermidis. J Clin Pathol 62: 1112-6. doi: 10.1136/jcp.2009.069021.
Cooper IR (2011) Microbial biofilms: case reviews of bacterial and fungal pathogens persisting on biomaterials and environmental substrata. In: Mendez-Vilas A, editor. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Badajoz, Spain: Formatex Research Centre: pp 807-17.
Costerton JW. (1999) Introduction to biofilm. Int J Antimicrob Agent 11: 217-221.
Dairy Australia (2011) Dairy Australia Strategic Plan 2011–2015. http://www.dairyaustralia.com.au/. Accessed December 2010.
Eddenden A, Kitova EN, Klassen JS, et al. (2020) An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation. ACS Chem Biol 15(5): 1204-1211. doi: 10.1021/acschembio.9b00907.
Fattom AI, Sarwar J, Ortiz A, et al. (1996) Staphylococcus aureus capsular polysaccharide (CP) vaccine and CP specific antibodies protect mice against bacterial challenge. Infect Immun 64: 1659– 1665.
Gad GFM, El-Feky MA, El-Rehewy MS, et al. (2009) Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients. J Infect Dev Ctries 3: 342-351.
Gal AE. (1968) Separation and identification of monosaccharides from biological materials by thin-layer choromatography. Anal Biochem 24: 452-461.
Geoghegan JA, Corrigan RM, Gruszka DT, et al. (2010) Role of surface protein SasG in biofilm formation by Staphylococcus aureus. J Bacteriol 197: 5663–5673.
Gökçen A, Vilcinskas A, Wiesner J. (2013) Methods to identify enzymes that degrade the main extracellular polysaccharide component of Staphylococcus epidermidis biofilms. Virulence 4(3): 260-70. doi: 10.4161/viru.23560.
Haddadin R, Saleh S, Al‐Adham I, et al. (2010) The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms. J Appl Microbiol 108: 1281-1291.
Han HR, Pak SI, Guidry A. (2000) Prevalence of capsular polysaccharide (CP) types of Staphylococcus aureus isolated from bovine mastitic milk and protection of S. aureus infection in mice with CP vaccine. J Vet Med Sci 62: 1331–1333.
Hemamalini V, Kavitha V, Ramachandran S. (2015) In vitro antibiogram pattern of Staphylococcus aureus isolated from wound infection and molecular analysis of mecA gene and restriction sites in methicillin resistant Staphylococcus aureus. J Adv Pharm Technol Res 6: 170.
Hsu CY, Shu JC, Lin MH, et al. (2015) High glucose concentration promotes vancomycin-enhanced biofilm formation of vancomycin-non-susceptible Staphylococcus aureus in diabetic mice. PloS One 10: 0134852.
Itoh Y, Wang X, Hinnebusch BJ, et al. (2005) Depolymerization of beta-1,6-N-acetyl-D-glucosamine disrupts the integrity of diverse bacterial biofilms. J Bacteriol 187(1): 382-7. doi: 10.1128/JB.187.1.382-387.2005. PMID: 15601723.
Kaplan JB. (2011) Antibiotic-induced biofilm formation. Int J Artif Organs 34: 737–751.
Kropec A, Maria-Litran T, Jefferson KK, et al. (2005) Poly-N-acetylglucosamine production in Staphylococcus aureus is essential for virulence in murine models of systemic infection. Infect Immun 73: 6868–6876
Ma Y, Xu Y, Yestrepsky BD, et al. (2012) Novel inhibitors of Staphylococcus aureus virulence gene expression and biofilm formation. PLoS One 7: e47255.
Maira-Laitren T, Kropec A, Goldmann D, et al. (2004) Biologic properties and vaccine potential of the staphylococcal Poly-N-acetyl glucosamine surface polysaccharide. Vaccine 22: 872–879.
Majidpour A, (2017) Dose-dependent effects of common antibiotics used to treat Staphylococcus aureus on biofilm formation. Iran J Pathol 12: 362.
Mann EE, Rice KC, Boles BR, et al. (2009) Modulation of eDNA release and degradation affects Staphylococcus aureus biofilm maturation. PLoS One 4: e5822.
Mirani ZA, Jamil N. (2011) Effect of sub-lethal doses of vancomycin and oxacillin on biofilm formation by vancomycin intermediate resistant Staphylococcus aureus. J Basic Microbiol 51:191-195.
Moori-Bakhtiari N, Moslemi M. (2017) Phenotypic evaluation of biofilm producing ability in Methicillin Resistant Staphylococcus aureus. Feyz 20: 525-31 [Article in Persian].
Mortensen NP, Fowlkes JD, Maggart M, et al. (2011) Effects of sub-minimum inhibitory concentrations of ciprofloxacin on enteroaggregative Escherichia coli and the role of the surface protein dispersin. Int J Antimicrob Agents 38: 27e34.
Naas HT, Edarhoby RA, Garbaj AM, et al. (2019) Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products and some seafood from Libyan retail markets. Vet World 12: 925.
Otani S, Hiramatsu K, Hashinaga K, et al. (2018) Sub-minimum inhibitory concentrations of ceftazidime inhibit Pseudomonas aeruginosa biofilm formation. J Infect Chemother 24: 428e433. https://doi.org/10.1016/j.jiac.2018.01.007
Pozzi C, Waters EM, Rudkin JK, et al. (2012) Methicillin resistance alters the biofilm phenotype and attenuates virulence in Staphylococcus aureus device-associated infections. PLoS Pathog 8: e1002626.
Quinn PJ, Carter ME, Markey B, et al. Clinical veterinary Microbiology. Lynton House, 7-12 Tavistock Square, London WC1H9LB, England: Mosby, 2012.
Roux D, Cywes-Bentley C, Zhang YF, et al. (2015) Identification of Poly-N-acetylglucosamine as a major polysaccharide component of the bacillus subtilis biofilm matrix. Int J Biol Chem 290: 19261−19272.
Ryder MA. (2005) Catheter-related infections: it’s all about biofilm. Top Adv Pract Nurs J 5: 1-6.
Sakai H, Procop G, Kobayashi N, et al. (2004) Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets. J Clin Microbiol 42: 5739-5744.
Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 2001.
Sohail M, Latif Z. (2018) Molecular analysis, biofilm formation, and susceptibility of methicillin-resistant Staphylococcus aureus strains causing community-and health care-associated infections in central venous catheters. Rev Soc Bras Med Trop 51: 603-609.
Stepanović S, Vuković D, Dakić I, et al. (2000) A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 40: 175-179.
Stepanović S, Vuković D, Hola V, et al. (2007) Quantification of biofilm in microtiter plates overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 115: 891-899.
Szczuka E, Urbańska K, Pietryka M, et al. (2013) Biofilm density and detection of biofilm-producing genes in methicillin-resistant Staphylococcus aureus strains. Folia Microbiologica 58: 47-52. doi: 10.1007/s12223-012-0175-9.
Takeda K, Akira S. (2003) Toll receptors and pathogen resistance. Cell Microbiol 5: 143-153.
Tormo MA, Ubeda C, Martí M, et al. (2007) Phase-variable expression of the biofilm-associated protein (Bap) in Staphylococcus aureus. Microbiology 153: 1702-1710.
Uymaz Tezel B, Ak¸celik N, Neslihan Yuksel F, et al. (2016) Effects of sub-MIC antibiotic concentrations on biofilm production of Salmonella Infantis. Biotechnol Biotechnol Equip 30: 1184–1191. http://dx.doi.org/10.1080/13102818.2016.1224981
Workineh S, Bayleyegn H, Mekonnen H, et al. (2002) Prevalence and aetiology of mastitis in cows from two major Ethiopian dairies. Trop Anim Health Prod 34: 19–25.
Wozniak DJ, Keyser R. (2004) Effects of sub-inhibitory concentrations of macrolide antibiotics on Pseudomonas aeruginosa. Chest 125: 62Se9S.