Ultrastructure of mice testes affected by orally administered crocin of crocus sativus


Δημοσιευμένα: Jan 18, 2024
Ενημερώθηκε: 2024-01-18
ARH Al-Fartwsy
AA Mohammadpour
A Parham
Περίληψη

In this study, thirty-two adult male mice were divided into four groups control and experimental treatment groups received crocin at 4, 20, and 100 mg/kg daily for six weeks. In results, testosterone level was increased significantly from 18.5 ng/dl in the control to 49.3 ng/dl in group received crocin at 20 mg/kg. The regular features of seminiferous tubules with increased Sertoli cells have been observed in the 20mg/kg crocin group.


 In the group received crocin at 100 mg/kg, many of the seminiferous tubules were atrophic, spermatogenic cells were discontinuous in the same areas while the space among them has been increased, and fewer interstitial tissue between seminiferous tubules were observed. The changes were markedly advanced by increasing the number of atrophied seminiferous tubules.


Electron microscopic results showed the large mitochondria with normal crista and dilated rough endoplasmic reticulum in spermatid cells of the 20 mg/kg crocin group.


In the 100 mg/kg crocin group, most of the cell cytoplasm showed vacuolated mitochondria with patchy matrix and distorted vision cristae, with many lysosomes, lipid droplets and vacuoles. Some sperms had abnormal swollen nuclear and irregular wavy acrosomal system.


The results of the present study showed that crocin at 20 mg/kg has a protective effect on the germinal epithelium of seminiferous tubules while at 100 mg/kg had destructive effects on the histological structure of the treated mice testicles.


In conclusion, treating healthy male mice with crocin for six weeks increased the testosterone concentration while at 100 mg/kg damaged the testicle’s structure

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Αναφορές
Abdullaev, F., & Espinosa-Aguirre, J. Biomedical properties of saffron
and its potential use in cancer therapy and chemoprevention trials.
Cancer Detect. Pre 2004;, 28(6), 426-432.
Abo Edra, N.M.M., Ahmed, A.A., El-Saka, M.H.,&El-Sawy, S.A. The
effect of crocin on endocrine functions of the testes before and after
testicular damage in rats. The Medical Journal of Cairo University
; 86(3), 375-382.
Asadi, M. H., Zafari, F., Sarveazad, A., Abbasi, M., Safa, M., Koruji, M.,
Miran, R. A. Saffron improves epididymal sperm parameters in rats
exposed to cadmium. Nephro-urology monthly 2014; 6(1).
Asdaq, S. M. B., & Inamdar, M. N. Potential of Crocus sativus (saffron)
and its constituent, crocin, as hypolipidemic and antioxidant in rats.
Appl. Biochem. Biotechnol 2010; 162(2), 358-372.
Ataei G, Rahbarian R, Rajabian Noghondar M. Effect of Crocin on Bax,
Bcl-2, and Oxidative Stress Markers in the Testis Tissue of Streptozotocin-Induced Diabetic Rats. Intern Med Today 2019; 25 (4) :340-351
Aung, H., Wang, C., Ni, M., Fishbein, A., Mehendale, S., Xie, J., Yuan,
C. Crocin from Crocus sativus possesses significant anti-proliferation
effects on human colorectal cancer cells. Experimental oncol 2007;
(3), 175.
Bakhtiary, Z., Shahrooz, R., Ahmadi, A., & Soltanalinejad, F. Protective
effects of crocin on testes of adult cyclophosphamide treated mice.
Armaghan Danesh 2014;19(184),12-23.
Bakhtiary, Z., Shahrooz, R., Ahmadi, A., & Zarei, L. Evaluation of antioxidant effects of crocin on sperm quqlity in cyclophosphamide treated
mice. Vet Res Forum2014; 5 (3) 213 - 218.
Bakshi, H. A., Sam, S., Feroz, A., Ravesh, Z., Shah, G. A., & Sharma, M.
Crocin from Kashmiri saffron (Crocus sativus) induces in vitro and
in vivo xenograft growth inhibition of Dalton’s lymphoma (DLA) in
mice. Asian Pac J Cancer Prev2009; 10(5), 887-890.
Bathaie, S. Z., & Mousavi, S. Z. New applications and mechanisms of
action of saffron and its important ingredients. Crit. Rev. Food Sci.
Nutr 2010;50(8), 761-786.
Bukhari, S.I.,& Manzoor. A comprehensive review of the pharmacological potential of crocus sativus and its bioactive apocarotenoides.Biomedicine & Pharmacotherapy, 2018;98(2):733-745.
Cao, P. C., Xiao, W. X., Yan, Y. B., Zhao, X., Liu, S., Feng, J., & Lei, W.
Preventive effect of crocin on osteoporosis in an ovariectomized rat
model. Evidence-Based Complementary and Alternative Medicine,
Chen, S., Zhao, S., Wang, X., Zhang, L., Jiang, E., Gu, Y., Yu, Z. (2015).
Crocin inhibits cell proliferation and enhances cisplatin and pemetrexed chemosensitivity in lung cancer cells. Transl Lung Cancer Res
; 4(6), 775.
Council, N. R. Guide for the care and use of laboratory animals.2010.
Davoodi, M., Zilaei Bouri, S & Dehghan Ghahfarokhi S. Aerobic training
and crocin consumption improve doxorubicin damage to spermatogenesis process and luteinizing hormone receptor expression of male
wistar rats.Jentashapir J Cell Mol Biol 2022;13(2):e127941.
Dianat, M., Radan, M., Badavi, M., Mard, S. A., Bayati, V., & Ahmadizadeh, M. Crocin attenuates cigarette smoke-induced lung injury
and cardiac dysfunction by anti-oxidative effects: the role of Nrf2
antioxidant system in preventing oxidative stress. Respir. Res 2018;
(1), 1-20.
Domínguez-Rebolledo, Á. E., Fernández-Santos, M. R., Bisbal, A.,
Ros-Santaella, J. L., Ramón, M., Carmona, M., Garde, J. J. Improving
the effect of incubation and oxidative stress on thawed spermatozoa
from red deer by using different antioxidant treatments. Reprod. Fertil. Dev 2010; 22(5), 856-870.
Fani, M., Mohammadipour, A., & Ebrahimzadeh-Bideskan, A. The effect
of crocin on testicular tissue and sperm parameters of mice offspring
from mothers exposed to atrazine during pregnancy and lactation periods: An experimental studyInt. J. Reprod. BioMed 2018; 16(8), 519.
Ganjiani, V., Ahmadi, N., Divar, M. R., Sharifiyazdi, H., &Meimandi-Parizi, A. (2021). Protective effects of crocin on testicular torsion/
detorsion in rats. Theriogenology, 173, 241-248.
Ghafarzadeh, S., Hobbenaghi, R., Tamaddonfard, E., Farshid, A. A., &
Imani, M.. Crocin exerts improving effects on indomethacin-induced small intestinal ulcer by antioxidant, anti-inflammatory and
anti-apoptotic mechanisms. Paper presented at the Vet. Res. Forum
;10(4),277-284.
Hadizadeh, F., Mohajeri, S., & Seifi, M. Extraction and purification of
crocin from saffron stigmas employing a simple and efficient crystallization method. Pak. J. Biol. Sci 2010; 13(14), 691-698.
Hosseinzadeh, H., Abootorabi, A., & Sadeghnia, H. R. (2008). Protective
effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on
methyl methanesulfonate-induced DNA damage in mice organs. DNA
and cell biology, 27(12), 657-664.
Hosseinzadeh, H., Shamsaie, F., & Mehri, S. (2009). Antioxidant activity
of aqueous and ethanolic extracts of Crocus sativus L. stigma and its
bioactive constituents, crocin and safranal. Pharmacognosy Magazine, 5(20), 419.
Jalili, C., Tabatabaei, H., Kakaberiei, S., Roshankhah, S., & Salahshoor,
M. R. Protective role of Crocin against nicotine-induced damages on
male mice liver. Int. j. prev. med. Res 2015; 6(1), 92.
Kamali, F. S., Shahrooz, R., Najafi, G. R., & Razi, M. (2020). Ameliorative effect of crocin on sperm parameters and in vitro fertilization in
mice under oxidative stress induced by paraquat. International Journal of Fertility & Sterility, 13(4), 307-314.
Khayatnouri, M., Safavi, S. E., Safarmashaei, S., Babazadeh, D., & Mikailpourardabili, B. . The effect of saffron orally administration on
spermatogenesis index in rat. Adv Environ Biol 2011; 5(7), 1514-
Khazdair, M. R., Boskabady, M. H., Hosseini, M., Rezaee, R., & Tsatsakis, A. M. The effects of Crocus sativus (saffron) and its constituents on nervous system: A review. AJP 2015; 5(5), 376.
Khorasani, M. K., Ahangarpour, A., & Khorsandi, L. Effects of crocin and
metformin on methylglyoxal-induced reproductive system dysfunction in diabetic male mice. Clin Exp Reprod Med 2021; 48(3), 221.
Lari, P., Abnous, K., Imenshahidi, M., Rashedinia, M., Razavi, M., &
Hosseinzadeh, H. Evaluation of diazinon-induced hepatotoxicity and
protective effects of crocin. Toxicol. Ind. Health 2015; 31(4), 367-
Licón, C., Carmona, M., Llorens, S., Berruga, M. I., & Alonso, G. L.
(2010). Potential healthy effects of saffron spice (Crocus sativus L.
stigmas) consumption. Funct Plant Sci Biotechnol, 4(S2), 64-73.
Lu, P., Lin, H., Gu, Y., Li, L., Guo, H., Wang, F., & Qiu, X. (2015). Antitumor effects of crocin on human breast cancer cells. International
journal of clinical and experimental medicine, 8(11), 20316.
Mard, S. A., Akbari, G., Mansouri, E., & Parsanahad, M. (2017). Renoprotective effect of crocin following liver ischemia/reperfusion injury
in Wistar rats. Iranian Journal of Basic Medical Sciences, 20(10),
Mesbahzadeh, B., Hassanzadeh-Taheri, M., Aliparast, M. S., Baniasadi,
P., & Hosseini, M. (2021). The protective effect of crocin on cisplatin-induced testicular impairment in rats. Bmc Urology, 21(1), 1-9.
Mirzaee, S., Bayatpoor, M. E., Shahyad, S., Mohammadi, M. T., & Bahari, Z. (2019). The protective effects of Crocin on testopathy in fat-fed
and streptozotocin-treated diabetic rats: An experimental study. International Journal of Reproductive BioMedicine, 17(2), 89-98.
Moallem, S. A., Afshar, M., Etemad, L., Razavi, B. M., & Hosseinzadeh,
H. (2016). Evaluation of teratogenic effects of crocin and safranal, active ingredients of saffron, in mice. Toxicology and industrial health,
(2), 285-291.
Modaresi, M., Messripour, M., Asadi Marghmaleki, M., & Hamadanian,
M. (2008). Effect of Saffron (Crocus Sativus) extract on level of FSH,
LH and testosterone in mice. Journal of Advances in Medical and
Biomedical Research, 16(63), 11-18.
Moghaddasi, M. S. (2010). Saffron chemicals and medicine usage. Journal of medicinal plants research, 4(6), 427-430.
Mohamadpour, A. H., Ayati, Z., Parizadeh, M. R., Rajbai, O., & Hosseinzadeh, H. (2013). Safety evaluation of crocin (a constituent of saffron) tablets in healthy volunteers. Iranian Journal of Basic Medical Sciences, 16(1), 39.
Pratap, S. A., & Rajender, S. Potent natural aphrodisiacs for the management of erectile dysfunction and male sexual debilities. Front. Biol
; 4, 167-180.
Qar, J., Al-Trad, B., Al Zoubi, M. S., Fayyad, D., Aljabali, A. A., Omari,
S. & Al-Batanyeh, K. (2022). Crocin treatment improves testosterone
induced benign prostatic hyperplasia in rats. Journal of Men’s Health,
(2), 38.
Raheem Hussein Al-Fartwsy A, Mohammadpour AA, Parham A. Evaluation of mice testosterone and sperm parameters affected with Crocin.
Comparative Clinical Pathology. 2022 ;31(1):109-14.
Rashedinia, M., Lari, P., Abnous, K., & Hosseinzadeh, H. Protective effect
of crocin on acrolein-induced tau phosphorylation in the rat brain.
Acta Neurobiol Exp 2015; 75(2), 208-219.
Roshankhah, S., Salahshoor, M., Jalili, F., Karimi, F., Sohrabi, M., & Jalili, C. Crocin effects on the nicotine-induce ovary injuries in female
rat. Int J Life Sci Pharm 2017; 7(4), 1-8.
Roshankhah, S., Jalili, C., & Salahshoor, M. R. (2019). Effects of Crocin on Sperm Parameters and Seminiferous Tubules in Diabetic Rats.
Journal of Advances in Medical and Biomedical Research, 8(4),
Roshankhah, S., Abdolmaleki, A., Jalili, C., &Salahshoor, M. R. (2019).
Crocin Reduces Oxidative Stress Produced by Busulfan Administration on Fertility of Male Rats. Journal of Advances in Medical and
Biomedical Research, 27(125), 25-33.
Safarinejad, M. R., Shafiei, N., & Safarinejad, S. A prospective double‐
blind randomized placebo‐controlled study of the effect of saffron
(Crocus sativus Linn.) on semen parameters and seminal plasma antioxidant capacity in infertile men with idiopathic oligoasthenoteratozoospermia. Phytother. Res 2011; 25(4), 508-516.
Salahshoor, M. R., Khazaei, M., Jalili, C., & Keivan, M. Crocin improves
damage induced by nicotine on a number of reproductive parameters
in male mice. Int. J. Fertil. Steril 2016; 10(1), 71.
Sefidgar, S. M., Ahmadi-Hamedani, M., Javan, A. J., Sani, R. N., & Vayghan, A. J. Effect of crocin on biochemical parameters, oxidative/
antioxidative profiles, sperm characteristics and testicular histopathology in streptozotocin-induced diabetic rats. AJP 2019; 9(4), 347.
Sun, J., Xu, X.-m., Ni, C.-z., Zhang, H., Li, X.-y., Zhang, C.-l., Zhou,
H..M. Crocin inhibits proliferation and nucleic acid synthesis and induces apoptosis in the human tongue squamous cell carcinoma cell
line Tca8113. Asian Pac J Cancer Prev 2011; 12(10), 2679-2683.
Tong, Y., Jiang, Y., Guo, D., Yan, Y., Jiang, S., Lu, Y., Wang, P. Homogenate extraction of crocins from saffron optimized by response surface
methodology. J Chem, 2018.
Vafaei, S., Motejaded, F., & Ebrahimzadeh-Bideskan, A. Protective effect
of crocin on electromagnetic field-induced testicular damage and heat
shock protein A2 expression in male BALB/c mice. Iran. J. Basic
Med. Sci 2020; 23(1), 102.
Veisi, A., Akbari, G., Mard, S. A., Badfar, G., Zarezade, V., & Mirshekar,
M. A. Role of crocin in several cancer cell lines: An updated review.
Iran. J. Basic Med. Sci 2020; 23(1), 3