Comparison of some biological activities of propolis and bee bread samples obtained from Apis mellifera Anatoliaca and its Muğla and Efe ecotypes

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Δημοσιευμένα: Jan 18, 2024
Ενημερώθηκε: 2024-01-18
DOI: https://doi.org/10.12681/jhvms.30859
D Kabakci
Department of Animal Production and Technologies, Faculty of Applied Sciences, Muş Alparslan University
Περίληψη

Honey and other bee products have been extensively researched in recent years due to the demand for natural products with nutritional and therapeutic qualities, as they contain high levels of biologically active compounds and valuable nutrients. In this study, antioxidant, anti-inflammatory and antimicrobial activities of bee bread and propolis samples formed after colony development by bees of different races and ecotypes accepted to core colonies in the same location were evaluated. Total phenolic, flavonoid and antioxidant content values were the the highest in propolis samples, especially in propolis from Apis mellifera anatoliaca. Values of this type gave the most effective results in almost all of the other tests performed, such as DPPH free radical scavenging, lipid peroxidation inhibition, anti-inflammatory and antimicrobial activity. In addition, the amount of phenolics detected in the phenolic component analysis of this species is higher. It was surprising that propolis samples obtained from Efe and Muğla ecotypes showed higher values, respectively, in the test for chelating activity with Fe. The fact that the values calculated for the bee breads were lower in all cases compared to the propolis samples, the highest values were calculated for the bee bread harvested from the efe ecotype, in contrast to the propolis ecotypes. None of the tested bee breads showed antimicrobial activity on selected pathogenic bacteria. Thus, with this study, it was evaluated how the differences arising from both race and ecotype and the type of bee product reflected on biological activities.

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Αναφορές
Ahmad FT, Lani MN, Nazari S A, Hajar NHM, Hassan KNAM, Razak
SBA, Hassan Z (2019) Antioxidant and antimicrobial properties of
honey, propolis and bee bread of stingless bee (Geniotrigona thoracica). Asian Journal Agriculture & Biology (1): 1-10.
Afonso AM, Gonçalves J, Luis A, Gallardo E, Duarte AP (2020) Evaluation of the In Vitro Wound-Healing Activity and Phytochemical
Characterization of Propolis and Honey.Applied Science 10(5): 1-14.
Bakour M, Fernandes A, Barros L, Sokovic M, Ferreira CFRI, Lyoussi
B (2019) Bee bread as a functional product: Chemical composition
and bioactive properties. LWT - Food Science and Technology (109):
-282.
Benzie IF, Strain JJ (1999) Ferric reducing antioxidant power assay: direct
measure of total antioxidant activity of biological fluids and modified
version for simultaneous measurement of total antioxidant power and
ascorbic acid concentration. Methods in Enzymology (299):15-27.
Borrelli F, Maffia P, Pinto L, Lanaro A, Russo A, Capasso F, Lalenti A
(2002) Phytochemical compounds involved in the anti-inflammatory
effect of propolis extract. Fitoterapia,73 (1): 53-63.
Bouarab -Chibane L, Forquet V, Lanteri P, Clement Y, Akkari LL, Oulahal
N, Degraeve P, Bordes C (2019) Antibacterial properties of polyphenols: characterization and QSAR (quantitative structure-activity relationship) models. Frontiers Microbiology(10): 829.
Bulman Z, Le P, Hudson AQ, Savka M A (2011) A novel property of propolis (bee glue): anti-pathogenic activity by inhibition of N-acyl-homoserine lactone mediated signaling in bacteria. Journal of Ethnopharmacology(138):788-797.
Braakhuis A (2019) Evidence on the Health Benefits of Supplemental
Propolis. Nutrients (11): 2705; doi:10.3390/nu11112705.
Cushnie, T P T, Lamb J A (2005) Antimicrobial activity off lavonoids.
International Journal of Antimicrobial Agents (26)343-356
Burdock G A (1998) Review of the Biological Properties and Toxicity of
Bee Propolis (Propolis). Food and Chemical Toxicology (36): 347-
Crane E (1990) Bees and beekeeping: science. practice and world resources. Heinemann Newnes 117-142.
Cruz KC, Ecker A, Dias R S, Seeger R L, Braga MM, Boligon AA, Martins IK, Costa- Silva DG, Barbosa NV, Canedo AD, Posser T, Franco
JL (2016) Brazilian Pampa Biome Honey Protects Against Mortality,
Locomotor Deficits and Oxidative Stress Induced by Hypoxia/ Reperfusion in Adult Drosophila melanogaster. Neurochemical Research
(41): 116 - 129.
Do Nascimento TG, Arruda R ES, Almeida ETC, Oliveira JMS, BasílioJúnior ID, Porto ICCM, Sabino AR, Tonholo J, Gray A, Ebel RAE,
Clements C, Zhang T, Watson DJ (2019) Comprehensive multivariate
correlations between climatic efect, metabolite-profle, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites
during seasonal study. Scientific Report (9): 1-16.
Dinis TCP, Madeira VMC, Almeida L M (1994) Action of phenolic derivatives (acetoaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics 315 (1): 161-169.
El Sohaimy SA, Masry S HD (2014) Phenolic content, antioxidant and
antimicrobial activities of Egyptian and Chinese propolis.American-Eurasian Journal Agriculture&Environment Science 1(14): 1116-
Eswaran VU, Bhargava HR (2014) Chemical analysis and anti-microbial
activity of Karnataka beebread of Apis species.World Apply Science
Journal(32): 379-385.
Filipič B, Rihar K, Exel Gregoric D, Gradisnik L, Pereyra A, Ruzic-Sabljic E, Galeotti F, Volpi N, Fachini A, Mazija H (2019) Propolis extracts enhance the antimicrobial activity of Slovenian: chestnut, forest
and flower honeys. Journal of Agricultural Science and Technology
(9): 203-219.
Ghisalberti E L (1979) Propolis: a Review Bee World 60(2): 59-84.
Gómez-Caravaca A M, Romero MG, Arraez-Roman D, Segura-Carretero A, Gutierrez A F (2006) Advances in the Analysis of Phenolic
Compounds in Products Derived from bees. Journal Pharmaceutical
Biomedical Analysis (41): 1220-1234.
Gonsales GZ, Orsi RO, Fernandes Júnior A, Rodrigues P, Funari SRC
(2006) Antibacterial activity of propolis collected in different regions
of Brazil. Journal of Venomous Animals and Toxins Including Tropical Diseases 12(2): 276-284.
Guzelmeriç E, Yülsel P I, Yaman Kadioğlu B, Sipahi H, Çelik C,
Kırmızıbekmez H, Aydın A, Yeşilada E (2021) Comparison of antioxidant and anti-inflammatory activity profiles of various chemically
characterized Turkish propolis sub-types: Which propolis type is a
promising source for pharmaceutical product development?Journal of
Pharmacetical and Biomaedical Analysis (203): 114196.
Gregoris E, Stevanato R (2010) Correlations between polyphenolic composition and antioxidant activity of Venetian propolis. Food and
Chemical Toxicology, (48): 76-82.
Imran M, Salehi B, Sharifi-Rad J, Gondal T A, Saeed F, Imran A, Shahbaz
M, Fokou PVT, Arshad MU, Khan H, Guerreiro SG, Martin N, Estevinho, L M (2019) Kaempferol: A Key Emphasis to Its Anticancer
Potential. Molecules (24): 2277.
Kara Y, Can Z, Kolaylı S (2022) Applicability of phenolic profile analysis
method developed with RP-HPLC-PDA to some bee product. Brazilian Archives of Biology and Technology 65.
Kasala ER, Bodduluru LN, Madana R M, Gogoi R, Barua CC (2015) Chemopreventive and therapeutic potential of chrysin in cancer: mechanistic perspectives. Toxicology letters 233(2): 214-225.
Kekeçoğlu M, Eroğlu N, Kambur M, Uçak (2020) The Relationships Between Propolis Collecting Capability and Morphometric Features of
Some Honey Bee Races and Ecotypes in Anatolia. Tarım Bilimleri
Dergisi 26(1): 71-76.
Kekeçoğlu M, Keskin M, Birinci C, Birinci E, Kolaylı S (2021) Effects
of Honey Bee Race and Season on Propolis Composition. Journal of
Agricultural Sciences 27 (3): 292-297.
Khalifa SAM, Elashal M, Kieliszek Ghazala NE, Farag MA, Saeed A,
Xiao J, Zou X, Khatib A, Göransson U, El-Seedi HR (2020) Recent
insights into chemical and pharmacological studies of bee bread.
Trends in Food Science and Technology 97: 300-316.
Kieliszek M, Piwowarek K, Kot A, Blazejak S, ChlebowskaSmigiel A,
Wolska I(2018). Pollen and bee bread as new healthoriented products:
A review. Trends in Food Science Technology(71): 170-180.
Kim D O, Chun OK, Moon YJ, Lee CY (2003) Quantification of Polyphenolics and Their Antioxidant Capacity in Fresh Plums. Jorurnal
Agriculture Food Chemistry 51: 6509.
Kolaylı S, Çakır HE, Şahin H, Can Zehra, Yıldız O (2016) Anti-Inflammatory Activities of Some Bee Products by Inhibition of Bovine Testes
Hyaluronidase. Current Enzyme İnhibition (12): 183 -187.
Kurek-Gorecka A, Rzepecka-Stojko A, Gorecki M, Stojko J, Sosada M,
Swierczek Zieba G(2014) Structure and antioxidant activity of polyphenols derived from propolis. Molecules. (19): 78-101.
Kumazawa S, Hamasaka T, Nakayama T (2004) Antioxidant activity of
propolis of various geographic origins.Food Chemistry (84): 329-
López-Romero JC, Gonzalez-Rios H, Pena-Ramos A, Velazquez C, Navarro M, Robles Zepeda R, Martinez-Benavidez E, Higuera-Ciapara
I, Virués C, Olivares JL, Domínguez Z, Hernández J (2018) Seasonal effect on the biological activities of Litsea glaucescens kunth
extracts. Evidence-based Complementary and Alternative Medicine(2738489): 11.
Ma S, Ma H, Pan Z, Luo L, Weng L (2016) Antioxidant activities of propolis’s 416 extracts by different solvents in vitro. Journal of Chinese
Institute of Food Science and Technology16 (8): 53-58.
Malkoç M, Çakır H, Kara Y, Zehra C, Kolaylı S (2019) Phenolıc Composıtıon And Antıoxıdant Propertıes Of Anzer Honey From Black Sea
Regıon Of Turkey. Uludağ Arı Dergisi 9 (2): 143 - 151.
Marcucci MC, Ferreres F, Garcia-Viguera C, Bankova VS, De Castro S L,
Dantas A P, Valented P H M, Paulino N (2001) Phenolic compounds
from Brazilian propolis with pharmacological activities.Journal of Ethnopharmacology (74): 105-112.
Martinello M, Mutinelli F (2021)Antioxidant Activity in Bee Products: A
Review.Antioxidants 10(1), 2-37.
Mobus B (1972) The importance of propolis to the honey bee. British Bee
Journal 19(8). 198-199.
Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hara H (2009) Comparison of bee products based on assays of antioxidant capacities.
BMC Complementary Medicine and Therapies. 9 (4). 1-9.
Newairy AS, Salama A F, Hüssien MH, Yousef M I (2009) Propolis alleviates aluminium-induced lipid peroxidation and biochemical parameters in male rats.Food and Chemical Toxicology, (47): 1093 - 1098.
Nyokat N, Yen K H, Hamzah A S, Lim IF, Saaidin AS (2017) Isolation
and synthesis of pinocembrin and pinostrobin from Artocarpus odoratissimus.Malaysian Journal of Analytical Sciences 21(5): 1156-1161.
Okińczyc P, Widelski J, Szperlik J, Zuk M, Mroczek T, Skalicka-Wozniak
K, Sakipova Z, Widelska G, Marek Kus P (2021) Impact of Plant
Origin on Eurasian Propolis on Phenolic Profile and Classical Antioxidant Activity. Biomolecules 11-18.
Othman Z A, Noordin L, Wan Ghazali WS, Omar G, Mahaneem M (2019)
Nutritional Phytochemical and Antioxidant Analysis of Bee Bread
from Different Regions of Malaysia. Indıan journal of Pharmaceutical
Sciences81(5):955 -960.
Orsolic N (2013) Possible Molecular Targets of Bee Venom in the Treatment of Cancer: Application and Perspectives.Forum Immunopathol
Disease (4): 275-231.
Padmavathi R, Senthilnathan P, Chodon D, Sakthisekaran D (2006) Therapeutic effect of paclitaxel and propolis on lipid peroxidation and antioxidant system in 7,12 dimethyl benz(a)anthracene-induced breast
cancer in female Sprague Dawley rats. Life Science (78): 2820 - 2825.
Prieto P, Pineda M, Anguilar M (1999) Spectrophotometric quantitation of
antioxidant capacity through the formation of a Phosphomolybdenum
complex: specific application to the determination of Vitamin E. Analytical Biochemistry269 (29): 337-341.
Peixoto M, Freitas AS, Cunha A, Oliveira R, Aguiar CA (2021) Antioxidant and antimicrobial activity of blends of propolis samples collected in different years. LWT - Food Science and Technology, (145),
Pryor WA, Cornicelli JA, Devall LJ, Tait B, Trivedi BK, Witiak DT, Wu
M (1993) A rapidscreening test to determine the antioxidant potencies
of natural and synthetic antioxidants. Journal of Organic Chemistry,
(13), 3521-3532.
Qaiyami S (2007) Macro- and Microdilution methods of antimicrobial
Suscebtibility Testing. In: Schwalbe, R., Steele-Moore, L., Goodwin,
A.C. (Eds.), Antimicrobial Suscebtibility Testing Protocols. Taylor
and francis Group 75-81. New York, USA
Ruttner F (1988) Biogeography and taxonomy of honey bees.Springer
Verlag Berlin 165-256.
Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to
measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture76 (2): 270-276.
Shehata MG, Ahmad FT, Badr AN, Masry SH, El Sohaimy SA (2020)
Chemical analysis antioxidant cytotoxic and antimicrobial properties
of propolis from different geographic regions. Annual Agricultural
sciences (65): 209-217.
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolibdic-phosphotungtic acid reagents.American Journal of
Enology and Viticulture 16 (3): 144 -158.
Soltani EK, Mokhnache K, Aichour SM, Charef N, Haro JP, Zerroug MM,
Esteban MA (2020) Chemical composition and antibacterial activity
of Algerian propolis against fish pathogenic bacteria. Journal of Drug
Delivery and Therapeutics 10(2): 12-19.
Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I,
Kassi E, Karabournioti S, Moutsatsou P (2014)Phenolic Acid Composition, Antiatherogenic and Anticancer Potential of Honeys Derived from Various Regions in Greece. Plosone 9 (4), 1 -10.
Suleiman JB, Mohamed M, Bakar ABA, Nna VU, Zakaria Z, Othman
ZA, Aroyehun AB (2021) Chemical Profile, Antioxidant Properties
and Antimicrobial Activities of Malaysian Heterotrigona itama Bee
Bread.Molecules 26 (16): 4943.
Sun C, Wu Z, Wang Z, Zhang H (2015) Effect of ethanol/water solvents
on phenolic profiles and antioxidant properties of Beijing propolis
extracts. Evidence-Based Complementary and Alternative Medicine
ID 595393.
Silici S (2014)Arı Poleni ve Arı Ekmeği.U Arı Dergisi Kasım 14 (2): 99-
Takaisi-Kikuni N, Schilcher H (1994) Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance.Planta Medical60 (3):
-227.
Usman UZ, Abu Bakar AB, Mohamed M (2016) Phytochemical screening
and 486 comparison of antioxidant activity of water and ethanol extract propolis from 487 Malaysia. International Journal of Pharmacy
and Pharmaceutical Sciences 8 (5): 488 -415.
Ürkü Ç, Önalan Ş, (2017) Determination of Lactococcus garvieae in Cultured Rainbow Trout by Different Diagnostic Tecniques. Aquaculture
Europa International Conference Exposition, Dubrovnik, Croata -
Hırvatistan, pp 98-99.
Valenzuela-Barra G, Castro C, Figueroa C, Barrigab A, Silvac X, Heras
B, Hortelano S, Delporte C (2015) Anti-inflammatory activity and
phenolic profile of propolis from two locations in Región Metropolitana de Santiago, Chile.Journal of Ethnopharmacology(168): 27-44.
Williams LAD, O’Connar A, Latore L, Dennis O, Ringer S, Whittaker
JA, Conrad J, Vogler B, Rosner H, Kraus W (2008) The in vitro anti-denaturation effects induced by natural products and non-steroidal
compounds in heat treated (immunogenic) bovine serum albumin is
proposed as a screening assay for the detection ofanti-inflammatorycompounds, without the use of animals, in the early stages of the drug
discovery process. West Indian Medicinal Journal 57 (4) 327-331.
Winston, ML (1991). The biology of the honey bee. Harvard University
press.
Yuan M, Yuan XJ, Pineda M, Liang ZY, He J, Sun SW, Pan TL, Li K P
(2020) A comparative study between Chinese propolis and Brazilian
green propolis: metabolite profile and bioactivity. Food Function 1
(11): 2368-2379.
Zaidi H, Ouchemıukh S, Ouchemoukh NA, Debbache N, Pacheco R,
Sarralheiro ML, Araujo ME (2019) Biological properties of phenolic
compound extracts in selected Algerian honeys—The inhibition of
acetylcholinesterase and α-glucosidase activities. European Journal
of Integrative Medicine 25: 77-84.
Zerdani I, Abouda Z, Kalalou I, Faid M, Ahami M (2011) The Antibacterial Activity of Moroccan Bee Bread and Bee-Pollen (Fresh and Dried)
against Pathogenic Bacteria.Research Journal of Microbiology( 6):
-384