Impact of oregano (Origanum vulgare) supplementation on antioxidant status, and related gene expression in Black Sea salmon, Salmo labrax

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Δημοσιευμένα: Jul 11, 2024
Ενημερώθηκε: 2024-07-11
Εκδόσεις:
2024-07-11 (2)
DOI: https://doi.org/10.12681/jhvms.31526
OT Özel
Central Fisheries Research Institute, Department of Aquaculture, Trabzon, Türkiye
ZD Düzgüneş
Central Fisheries Research Institute, Department of Breeding and Genetics, Trabzon, Türkiye
SΕ Gürkan
Çanakkale Onsekiz Mart University, Faculty of Science, Department of Biology, Çanakkale, Türkiye
E Çakmak
Central Fisheries Research Institute, Department of Aquaculture, Trabzon, Türkiye
Περίληψη

The aim of this study was to to evaluate the effect of diets containing 0, 50, 100, 200, and 400 mg kg-1 oregano (Origanum vulgare) essential oil on antioxidant enzymes (CAT, GPx and SOD) in liver and muscle tissues and expression levels of their-relevant genes of Black Sea salmon (Salmo labrax) juvenile. This study was conducted in the freshwater recirculating aquaculture system (RAS). Fish with average initial weights of 3.52±0.01g were distributed randomly in triplicate to 50 liter fiberglass tanks, and 45 fish were placed in each experimental tank. Fish were fed 3% level of live weight for 90 days. The addition of oregano essential oil had no significant impacts on CAT in liver and GPx in muscle (p>0.05), but increased SOD activity was observed in muscle. The increased GPx genes expression were observed in both muscle and liver tissues. The addition of oregano essential oil had no significant impacts on the mRNA expression of SOD in muscle, nevertheless increased SOD genes expression were determined in liver. In terms of the mRNA expression of CAT, control and 200 mg kg-1 concentration were similar to each other and had the highest level. Present study suggests that the addition of oregano essential oil can enhanced the antioxidant enzyme activities, and related gene expression of Black Sea salmon (Salmo labrax) juvenile.

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Αναφορές
Abd El-Naby AS, Samir F, Abdel Razek N, Khattaby AA (2017) Effect
of aquaviance product as dietary supplementation to improve growth
performance, feed intake, innate immunity and antioxidant activity
for Nile tilapia, (Oreochromis niloticus). Abbassa Int. J. Aqua. vol.10
No.(1).
Abdel-Latif HMR, Khalil RH (2014) Evaluation of two Phytobiotics,
Spirulina platensis and Origanum vulgare extract on Growth, Serum
antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. International Journal of
Fisheries and Aquatic Studies, 1(5): 250-255.
Abdel-Latif HMR, Abdel-Tawwab M, Khafaga AF, Dawood MAO (2020)
Dietary oregano essential oil improved antioxidative status, immune-related genes, and resistance of common carp (Cyprinus carpio
L.) to Aeromonas hydrophila infection. Fish and Shellfish Immunology, https://doi.org/10.1016/j.fsi.2020.05.056.
Abdelkhalek NK, Risha E, El-Adl MA, Salama MF, Dawood MA O
(2020) Antibacterial and antioxidant activity of clove oil against
Streptococcus iniae infection in Nile tilapia (Oreochromis niloticus)
and its effect on hepatic hepcidin expression. Fish and Shellfish Immunology, 104, 478-488. https://doi.org/10.1016/j.fsi.2020.05.064.
Abdel-Tawwab M, Samir F, Abd El-Naby AS, Monier MN (2018a)
Antioxidative and immunostimulatory effect of dietary cinnamon
nanoparticles on the performance of Nile tilapia, Oreochromis niloticus (L.) and its susceptibility to hypoxia stress and Aeromonas
hydrophila infection. Fish and Shellfish Immunology, https://doi:
1016/j.fsi.2017.12.033.
Asadi MS, Gharaei A, Harijani JM, Arshadi A (2018) A Comparison between dietary effects of Cuminum cyminum essential oil and Cuminum cyminum essential oil, loaded with iron nanoparticles, on growth
performance, immunity and antioxidant indicators of white leg
shrimp (Litopenaeus vannamei). Aquaculture nutrition, 1-8. https://
doi: 10.1111/anu.12683.
Azambuja CR, Mattiazzi J, Riffel APK, Finamor IA, Garcia LO, Heldwein CG, Heinzmann BM, Baldisserotto B, Pavanato MA, Llesuy
SF (2011) Effect of the essential oil of Lippia alba on oxidative
stress parameters in silver catfish (Rhamdia quelen) subjected to
transport. Aquaculture, 319, 156-161. https://doi:10.1016/j.aquaculture.2011.06.002.
Baldissera MD, Souza CF, Moreira KLS, Rocha MIUM, Veiga ML,
Baldisserotto B (2017a) Melaleuca alternifolia essential oil prevents
oxidative stress and ameliorates the antioxidant system in the liver
of silver catfish (Rhamdia quelen) naturally infected with Ichthyophthirius multifiliis. Aquaculture, 480, 11-16. https://doi.org/10.1016/j.
aquaculture.2017.07.042.
Baldissera MD, Souza CF, Júnior GB, Vargas AC, Boligon AA, Campos
MMA, Stefani LM, Baldisserotto B (2017b) Melaleuca alternifolia
essential oil enhances the non-specific immune system and prevents
oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila: Effects on cholinergic and purinergic systems in
liver tissue. Fish and Shellfish Immunology, https://doi: 10.1016/j.
fsi.2016.12.016.
Bradford MM (1976) A rapid sensitive method for the quantification of
microgram quantities of protein utilizing the principle of protein-dye
binding. Analytical biochemistry, 72, 248-254.
Beltran JMG, Silvera DG, Ruiz CE, Campo V, Chupani L, Faggio C, Esteban MA (2020) Effects of dietary Origanum vulgare on gilthead
seabream (Sparus aurata L.) immune and antioxidant status. Fish
and Shellfish Immunology, 99, 452-461. https://doi.org/10.1016/j.
fsi.2020.02.040.
Cagol L, Baldisserotto B, Becker AG, Souza CF, Heinzmann BM, Caron
BO, Leona FA, Santos LD, Ballester ELC (2020) Essential oil of Lippia alba in the diet of Macrobrachium rosenbergii: Effects on antioxidant enzymes and growth parameters. Aquaculture research, 00:1-9.
https://doi: 10.1111/are.14569.
Clairborne A (1985) Catalase Activity. In: Grenwald, R.A., Eds. Handbook of Methods of Oxygen Radical Research. CRC Press, Boca Raton, FL., 283-284.
Dokou S, Bitchava K, Stylianaki I, Chantzi P, Efstathiou A, Vasilopoulou K, Tsoumani M, Gouva E, Michailidis G, Prafulla K, Karagouni
E, Giannenas I (2023) Nano-oregano essential oil improves rainbow
trout’s (Oncorhynchus mykiss) growth performance, oxidative status, fatty acid profile of fillet, affects gene expression and supports
skin and intestinal histomorphometry. Annals of Animal Science, doi:
2478/aoas-2023- 0032.
European Commission (2010) Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection
of animals used for scientific purposes. The Official Journal of the
European Union, 276, 1-79.
Flöhe L, Ötting F (1984) Superoxide dismutase assays. Methods of enzymology, 105, 93-104.
Giannenas I, Triantafillou E, Stavrakakis S, Margaroni M, Mavridis S,
Steiner T, Karagouni E (2012) Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance, intestinal microbiota and antioxidant status of rainbow trout
(Oncorhynchus mykiss). Aquaculture, 350-353, 26-32. doi:10.1016/j.
aquaculture.2012.04.027.
Hassaan MS, Mohammady EY, Soaudy MR, El-Garhy HAS, Moustafa
MMA, Mohamed SA, El-Haroun ER (2019) Effect of Silybum marianum seeds as a feed additive on growth performance, serum biochemical indices, antioxidant status, and gene expression of Nile tilapia,
Oreochromis niloticus (L.) fingerlings. Aquaculture, 509, 178-187.
https://doi.org/10.1016/j.aquaculture.2019.05.006.
He W, Rahimnejad S, Wang K, Song K, Lu K, Zhang C (2017) Effects of
organic acids and essential oils blend on growth, gut microbiota, immune response and disease resistance of Pacific white shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus. Fish & Shellfish
Immunology, 70, 164e173. https://doi.org/10.1016/j.fsi.2017.09.007.
Jafarinejad R, Gharaei A, Mirdarf HJ (2020) Dietary ginger improve
growth performance, blood parameters, antioxidant capacity and
gene expression in Cyprinus carpio. Iranian Journal of Fisheries Sciences, 19(3) 1237-1252. https://doi: 10.22092/ijfs.2018.119876.
Khafaga AF, Naiel MAE, Dawood MAO, Abdel-Latif HMR (2020) Dietary Origanum vulgare essential oil attenuates cypermethrin-induced
biochemical changes, oxidative stress, histopathological alterations,
apoptosis, and reduces DNA damage in Common carp (Cyprinus
carpio). Aquatic Toxicology, 228, 105624. https://doi.org/10.1016/j.
aquatox.2020.105624.
Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG (2010)
Improving bioscience research reporting: The ARRIVE guidelines
for reporting animal research. PLoS Biology, 8, e1000412. https://
doi:10.1371/journal.pbio.1000412.
Lopes JM, Marques NC, Santos MDM, Souza CF, Baldissera MD, Carvalho RC, Santos L. L, Pantoja BTS, Heinzmann BM, Baldisserotto B (2020) Dietary limon Citrus × latifolia fruit peel essential oil
improves antioxidant capacity of tambaqui (Colossoma macropomum) juveniles. Aquaculture research, 00:1-11. https://doi: 10.1111/
are.14771.
Mirghaed AT, Hoseini SM, Ghelichpour M (2018) Effects of dietary
,8-cineole supplementation on physiological, immunological and
antioxidant responses to crowding stress in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 81, 182-188. https://doi.org/10.1016/j.fsi.2018.07.027.
Mirghaed AT, Hoseini SM, Hoseinifar SH, Doan HV (2020) Effects of
dietary thyme (Zataria multiflora) extract on antioxidant and immunological responses and immune-related gene expression of rainbow
trout (Oncorhynchus mykiss) juveniles. Fish and Shellfish Immunology, 106, 502-509. https://doi.org/10.1016/j.fsi.2020.08.002.
Mohamed RA, Yousef YM, El-Tras WF, Khalafallaa MM (2020) Dietary
essential oil extract from sweet orange (Citrus sinensis) and bitter
lemon (Citrus limon) peels improved Nile tilapia performance and
health status. Aquaculture research, 00:1-17. https://doi: 10.1111/
are.15000.
Mousavi S, Sheikhzadeh N, Tayefi-Nasrabadi H, Alizadeh-Salteh S,
Oushani AK, Firouzamandi M, Mardani K (2020) Administration of
grape (Vitis vinifera) seed extract to rainbow trout (Oncorhynchus
mykiss) modulates growth performance, some biochemical parameters, and antioxidant-relevant gene expression. Fish Physiol Biochem., 46:777-786. https://doi.org/10.1007/s10695-019-00716-4.
Ning L, Zhang X, Zhang D, Hu Y, Li Y (2020) The benefits of blend essential oil for GIFT tilapia on the digestion, antioxidant, and muscle
quality during cold storage. Aquaculture, 533, 736097. https://doi.
org/10.1016/j.aquaculture.2020.736097.
Olsvik PA, Kristensen T, Waagbø R, Rosseland BO, Tollefsen KE,
Baeverfjord G, Berntssen MHG (2005) mRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress
in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water
during smoltification. Comparative Biochemistry and Physiology,
Part C 141, 314 - 323. https://doi: 10.1016/j.cbpc.2005.07.009.
Pfaffl MW (2001) A new mathematical model for relative quantification in
real-time, RT-PCR. Nucleic Acids Res., 29, 2003-2007.
Ribeiro SC, Malheiros DF, Guilozki IC, Majolo C, Chaves FCM, Chagas
EC, Assis HCS, Tavares-Dias M, Yoshioka ETO (2018) Antioxidants
effects and resistance against pathogens of Colossoma macropomum (Serassalmidae) fed Mentha piperita essential oil. Aquaculture,
https://doi:10.1016/j.aquaculture.2018.02.024.
Saccol EMH, Uczay J, Pes TS, Finamor IA, Ourique GM, Riffel APK,
Schmidt D, Caron BO, Heinzmann BM, Llesuy SF, Lazzari R, Baldisserotto B, Pavanato, MA (2013) Addition of Lippia alba (Mill) N.
E. Brown essential oil to the diet of the silver catfish: An analysis
of growth, metabolic and blood parameters and the antioxidant response. Aquaculture, 416-417, 244-254. https://doi.org/10.1016/j.
aquaculture.2013.09.036.
Safari R, Hoseinifar SH, Van Doan H, Dadar M (2017) The effects of dietary Myrtle (Myrtus communis) on skin mucus immune parameters
and mRNA levels of growth, antioxidant and immune related genes in
zebrafish (Danio rerio), Fish and Shellfish Immunology, https://doi:
1016/j.fsi.2017.05.007.
Sun Z, Tan X, Ye H, Zou G, Ye G, Wang A (2018) Effects of dietary Panax notoginseng extract on growth performance, fish composition,
immune responses, intestinal histology and immune related genes expression of hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus
fuscoguttatus ♀) fed high lipid diets. Fish and Shellfish Immunology,
, 234-244. https://doi.org/10.1016/j.fsi.2017.11.007.
Wendel A (1980) Glutathione Peroxidase. In: Jakoby, W.B., (Eds.) Enzymatic Basis of Detoxification I. Academic Press, New York. 333-353.
Yousefi M, Ghafarifarsani H, Hoseinifar SH, Rashidian G, Van Doan H
(2020) Effects of dietary marjoram, Origanum majorana extract on
growth performance, hematological, antioxidant, humoral and mucosal immune responses, and resistance of common carp, Cyprinus
carpio against Aeromonas hydrophila, Fish and Shellfish Immunology, doi: https://doi.org/10.1016/j.fsi.2020.11.019.
Zhang R, Wang XW, Liu LL, Cao YC, Zhu H (2019) Dietary oregano
essential oil improved the immune response, activity of digestive enzymes, and intestinal microbiota of the koi carp, Cyprinus carpio.,
Aquaculture, https://doi.org/10.1016/j.aquaculture.2019.734781.
Zeppenfeld CC, Saccol EMH, Pes TS, Salbego J, Koakoski G, dos Santos
AC, Heinzmann BM, da Cunha MA, Barcellos LJG, Pavanato MA,
Caron BO, Baldisserotto B (2016) Aloysia triphylla essential oil as
food additive for Rhamdia quelen - Stress and antioxidant parameters. Aquaculture nutrition, 1-6. https://doi: 10.1111/anu.12511.
Zheng ZL, Tan JYW, Liu HY, Zhou XH, Xiang X, Wang KY (2009)
Evaluation of oregano essential oil (Origanum heracleoticum L.) on
growth, antioxidant effect and resistance against Aeromonas hydrophila in channel catfish (Ictalurus punctatus). Aquaculture, 292, 214-
https://doi:10.1016/j.aquaculture.2009.04.025.
Zou Y, Wang J, Peng J, Wei H (2016) Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2
Cells. Oxidative Medicine and Cellular Longevity, https://doi.
org/10.1155/2016/5987183.