The Effect of the addition of pre-fermented juice and soluble carbohydrates to alfalfa silage on silage quality

PDF (English)
Δημοσιευμένα: Jul 9, 2024
Εκδόσεις:
2024-07-09 (1)
DOI: https://doi.org/10.12681/jhvms.32344
SS Aydin
Faculty of Veterinary Medicine, University of Harran Sanliurfa, Turkey
https://orcid.org/0000-0002-3252-3944
Περίληψη

In this study, it was aimed to determine the effects of fermented natural lactic acid bacteria (LAB) and different soluble carbohydrate sources on silage quality characteristics, in vitro organic matter digestion (IVOMD), metabolic energy (ME) and in vitro methane gas formation in alfalfa (Medicago sativa L.) silage. In the study, an analysis (pH, total LAB, yeast, mold, LA, AA, and LA/AA) was conducted on the naturally fermented lactic acid bacteria liquid prepared from alfalfa plants with a 3% molasses addition. While the silage prepared from the pure alfalfa plant in the study constituted the control group (i), the experimental groups; (ii) PFJ + pure alfalfa silage, (iii) PFJ + 1.5% molasses added alfalfa silage, (iv) PFJ + 1.5% fructose added alfalfa silage, (v) PFJ + 1.5% sucrose added alfalfa silage. In the study, the differences between the groups were statistically significant for the dry matter (DM), acid detergent insoluble fiber (ADF), IVOMD, metabolic energy (ME) and methane (CH4) values. When the fermentation characteristics (pH, NH3-N, LA, AA, LA/AA, LA/AA, and yeast-mold after aerobic stability) of the prepared silages were examined, the differences between the groups were found to be statistically significant. When analyzed in terms of all parameters, it was determined that the addition of PFJ + 1.5% sucrose added alfalfa silage had positive effects on silage quality, the fermentation characteristics and in vitro organic matter digestion

Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Creative Commons License

Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.

Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.

Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.

Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc

Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.

Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Açıkgöz E, Turgut İ, Filya İ (2011) Silage crops and production. İstanbul,
Hasad yayıncılık.
Ali N, Wang S, Zhao J, Dong Z, Li J, Nazar M, Shao T (2020) Microbial
diversity and fermentation profile of red clover silage inoculated with
reconstituted indigenous and exogenous epiphytic microbiota. Bioresour Technol 314:123606.
AOAC (2005) Official methods of the association of official analytical
chemists. 16th edn. Arlington, TX: Association of Official Analytical
Chemists.
Ashbell G, Weinberg Z G, Azrieli A, Hen Y, Horev B (1991) A simple
system to study the aerobic determination of silages. Can Agric Eng
:171-175.
Aydin S S, Denek N (2019) The effect of fermented lactic acid juice prepared with different levels of sucrose and incubation times on the
alfalfa silage quality. Harran Üniv Vet Fak Derg 8(1): 44-51.
Aydın S S (2022) The effect of various additives addition to barley-vetch
mixtures on silage quality characteristics and in vitro digestibility.
ISPEC J Agric Sci 6(4): 743-751.
Aydin S S, Denek N (2022) Amount of lactic acid bacteria in fermented
natural lactic acid bacteria liquids prepared with varying sucrose inclusion at different incubation periods. Med Weter 78(9): 456-460.
Aydin S S, Denek N, Kırar N, Top Ş, Baytur G (2022). Effects of different
silage additives on silage quality of gramineae forage mixtures. MAS
J Appl Sci 7(3): 570-578.
Bezerra H F C, Santos E M, Oliveira J S, Carvalho G G P, Pinho R M A,
Silva T C, ... & Zanine A M (2019) Fermentation characteristics and
chemical composition of elephant grass silage with ground maize and
fermented juice of epiphytic lactic acid bacteria. S Afr J Anim Sci
(3): 522-533.
Bingöl N T, Bolat D, Karslı M A, Akça İ (2009) Effects of molasses addition into barley-sainfoin mixture at varying levels on silage quality
and digestibility. Atatürk Univ J Vet Sci 4(1):23-30. (in Turkish with
an abstract in English)
Boga M, Ayasan T (2022). Determination of nutritional value of alfalfa
varieties and lines by using the in vitro method and gas production
technique. J Hellenic Vet Med Soc 73(1): 3613-3620.
Bolsen K K, Ashbell G, Weinberg Z G (1996) Silage fermentation and
silage additives-Review. Asian Australas J Anim Sci 9(5): 483-494.
Broderick G A, Kang J H (1980) Automated simultaneous determination
of ammonia and total amino acids in ruminal fluid and in vitro media.
J Dairy Sci 63(1): 64-75.
Bureenok S, Namihira T, Tamaki M, Mizumachi S, Kawamoto Y, Nakada
T (2005a) Fermentative quality of guineagrass silage by using fermented juice of the epiphytic lactic acid bacteria (FJLB) as a silage
additive. Asian Australas J Anim Sci 18(6): 807-811
Bureenok S, Namihira T, Kawamoto Y, Nakada T (2005b) Additive effects
of fermented juice of epiphytic lactic acid bacteria on the fermentative quality of guineagrass (Panicum maximum Jacq.) silage. Grassl
Sci 51(3): 243-248.
Çayıroğlu H, Coşkun İ, Şahin A (2016) Factors affecting the aerobic
stability of silage and improvement strategies. Alınteri J Agric Sci
(31B): 91-97.
Çotuk G (2016) Effect of wheat bran and pudding added to alfalfa silage on fermentati̇on, aerobi̇c stability and in vitro digestibility. MSc
Thesis. Namık Kemal University, Graduate School of Natural and
Applied Sciences, Tekirdağ, Türkiye. (in Turkish with an abstract in
English)
Denek N, Can A, Avci M, Aksu T, Durmaz H (2011) The effect of molasses‐based pre‐fermented juice on the fermentation quality of first‐cut
lucerne silage. Grass Forage Sci 66(2): 243-250.
Drouin P, Mari L J, Schmidt R J (2019) Lactic acid bacteria as microbial
silage additives: current status and future outlook. In New Advances
on Fermentation Processes. IntechOpen 266.
Fijałkowska M, Przemieniecki S W, Purwin C, Lipiński K, Kurowski T
P, Karwowska A (2020) The effect of an additive containing three
Lactobacillus species on the fermentation pattern and microbiological
status of silage. J Sci Food Agric 100(3): 1174-1184.
Filya I, Ashbell G, Hen Y, Weinberg Z G (2000) The effect of bacterial
inoculants on the fermentation and aerobic stability of whole crop
wheat silage. Anim Feed Sci Technol 88(1-2): 39-46.
Gao R, Wang B, Jia T, Luo Y, Yu Z (2021) Effects of different carbohydrate sources on alfalfa silage quality at different ensiling days.
Agriculture 11(1): 58.
Gollop N, Zakin V, Weinberg Z G (2005) Antibacterial activity of lactic
acid bacteria included in inoculants for silage and in silages treated
with these inoculants. J Appl Microbiol 98(3): 662-666.
Güney F, Ertürk Ö (2020) Determination of the effects of propolis ethanolic extract on some properties of fruit yoghurt during storage. MKU
J Agric Sci 25(2): 145-152.
Güney M, Kale C, Temur C, Bingol N T, Aksu T (2018) The effect of
molasses and pre-fermented lactic acid juice on silage quality and digestibility of sugar beet pulp+ wheat straw silage. Fresenius Environ
Bull 27(11):7454-7459.
Kızılşimşek M, Erol A, Dönmez R., Katrancı B (2016a) Relationship
among silage micro flora and their effects on silage fermentation and
quality. KSU J Nat Sci 19(2):136-140.
Kızılşimşek M, Küsek M, Gezginç Y, Erol A (2016b) Isolation and identification of high lactic acid producer bacteria from forage and their
silages grown in different ecologies. Kafkas Univ Vet Fak Derg 22(2):
-296.
Koç F, Aksoy S O, Okur A A, Çelikyurt G, Korucu D, Özduven M L
(2017) Effect of pre-fermented juice, Lactobacillus plantarum and
Lactobacillus buchneri on the fermentation characteristics and aerobic stability of high dry matter alfalfa bale silage. The J Anim Plant
Sci 27(6): 1766-1773.
Korkut S (2016) Research of the mechanization of silage maize in Kırklareli. MSc Thesis. Namık Kemal University, Graduate School of
Natural and Applied Sciences, Tekirdağ, Türkiye (in Turkish with an
abstract in English)
Kung Jr L (2010, December). Understanding the biology of silage preservation to maximize quality and protect the environment. In Proceedings, California Alfalfa & Forage Symposium and Corn/Cereal
Silage Conference (pp. 1-2). Visalia, CA: University of California,
Davis, CA.
Kung L, Shaver R (2021) Interpretation and use of silage fermentation
analysis reports. Focus on Forage 3(13): 1-5.
Liu Q H, Dong Z H, Shao T (2018). Effect of additives on fatty acid profile
of high moisture alfalfa silage during ensiling and after exposure to
air. Anim Feed Sci Technol 236: 29-38.
Luo R, Zhang Y, Wang F, Liu K, Huang G, Zheng N, Wang J (2021) Effects of sugar cane molasses addition on the fermentation quality, microbial community, and tastes of alfalfa silage. Animals 11:335 11(2):
Masuko T, Hariyama Y, Takahashi Y, Cao L M, Goto M, Ohshima M
(2002) Effect of addition of fermented juice of epiphytic lactic acid
bacteria prepared from timothy (Phleum pratense) and orchardgrass
(Dactylis glomerata) on fermentation quality of silages. J Japan Soc
Grassl Sci 48(2):120-125.
Menke K H, Raab L, Salewski A, Steingass H, Fritz D, Schneider W
(1988) Estimation of the energetic feed value obtained from chemical
analysis and in vitro gas production using rumen fluid. Anim Res Dev
: 7-55.
Miralestari M, Sudarman A, Suharti S, Sofyan A (2021) Enhancing physical-chemical quality and palatability of king grass (Pennisetum
Hyrid) silage treated by combination of water soluble carbohydrate
and legume sources. ICEST 2018 - 3rd International Conference of
Computer, Environment, Agriculture, Social Science, Health Science,
Engineering and Technology. 270-275.
Ohshima M, Kimura E, Yokota H O (1997) A method of making good
quality silage from direct cut alfalfa by spraying previously fermented juice. Anim Feed Sci Technol 66(1-4): 129-137.
Playne M J, McDonald P (1966) The buffering constituents of herbage and
of silage. J Sci Food Agric 17(6): 264-268.
Polan C E, Stieve D E, Garrett J L (1998) Protein preservation and ruminal
degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant. J Dairy Sci 81(3): 765-776.
Şen G, Erol T, Kara K, Demirci M, Karsli M A (2022). The effect of microbial inoculants and molasses on quality and in vitro digestibility of
silages prepared with different proportions of ryegrass and Hungarian
vetch. Turkish J Vet Anim Sci 46(4): 629-637.
Seydoşoğlu S (2019) Investigation of the effect of fodder pea (Pisum sativum L.) and barley (Hordeum vulgare L.) herbages mixed at different rates on silage and feed quality. Ege Üniv Ziraat Fak Derg 56(3):
-302.
Silva V P, Pereira O G, Leandro E S, Paula R A, Agarussi M C, Ribeiro K
G (2020) Selection of lactic acid bacteria from alfalfa silage and its
effects as inoculant on silage fermentation. Agriculture. 10(11): 518.
Soundharrajan I, Muthusamy K, Han O K, Lee H J, Purushothaman S,
Kim D, Choi K C (2020) Effects of microbial inoculants on the fermentation and preservation of triticale silages at high and low moisture levels. Appl Sci 10(21): 7855.
Soundharrajan I, Park H S, Rengasamy S, Sivanesan R, Choi K C (2021)
Application and future prospective of lactic acid bacteria as natural
additives for silage production—a review. Appl Sci 11(17): 8127.
SPSS (1991) Inc. Statistical package for the social sciences (SPSS/PC+).
Chicago, IL.
Sun L, Jiang Y, Ling Q, Na N, Xu H, Vyas D, ... & Xue Y (2021) Effects
of adding pre-fermented fluid prepared from red clover or lucerne
on fermentation quality and in vitro digestibility of red clover and
lucerne silages. Agriculture 11(5): 454.
Sun Y, Yang Q (2021) Evaluation of the performance of seventeen varieties of alfalfa (Medicago sativa L). XXI International Grassland
Congress /VIII International Rangeland Congress.
Suzuki M, Lund C W (1980) Improved gas-liquid chromatography for
simultaneous determination of volatile fatty acids and lactic acid in
silage. J Agric Food Chem 28(5): 1040-1041.
Tao L, Zhou H, Zhang N, Si B, Tu Y, Ma T, Diao Q (2017) Effects of
different source additives and wilt conditions on the pH value, aerobic stability, and carbohydrate and protein fractions of alfalfa silage.
Anim Sci J 88(1): 99-106.
Turan A (2015). Effect of cumin essential oil usage on fermentation quality and aerobic stability of alfalfa silage. MSc Thesis. Namık Kemal University, Graduate School of Natural and Applied Sciences,
Tekirdağ, Türkiye. (in Turkish with an abstract in English)
Turan A, Önenç S S (2018) Effect of cumin essential oil usage on fermentation quality, aerobic stability and in vitro digetibility of alfalfa
silage. Asian Australas J Anim Sci 31(8): 1252.
Van Soest P V, Robertson J B, Lewis B A (1991) Methods for dietary fiber,
neutral detergent fiber, and nonstarch polysaccharides in relation to
animal nutrition. J Dairy Sci 74(10): 3583-3597.
Yıldırım Ö, Aydın S S, Korkmaz Ö, Korkmaz D, Demircioğlu İ, Kırar N,
... & Tekçe A (2022) Effects of probiotic application on oviduct and
ovarian morphology in quails under heat stress. Etlik Vet Mikrobiyol
Derg 33(1): 89-96.
Zhang L, Li X, Wang S, Zhao J, Dong Z, Zhao Q, ... & Shao T (2022). Effect of sorbic acid, ethanol, molasses, previously fermented juice and
combined additives on ensiling characteristics and nutritive value of
napiergrass (Pennisetum purpureum) silage. Fermentation 8(10): 528.
Zhang Y X, Ke W C, Bai J, Li F H, Xu D M, Ding Z T, Guo X S (2020)
The effect of Pediococcus acidilactici J17 with high-antioxidant activity on antioxidant, α tocopherol, β carotene, fatty acids, and fermentation profiles of alfalfa silage ensiled at two different dry matter
contents. Anim Feed Sci Technol 268: 114614.
Zhang Y G, Xin H S, Hua J L (2010) Effects of treating whole-plant or
chopped rice straw silage with different levels of lactic acid bacteria on silage fermentation and nutritive value for lactating Holsteins.
Asian Australas J Anim Sci 23(12): 1601-1607.