Investigation of the presence of equine herpesvirus-1 (EHV-1) in tissue samples of aborted foals by histopathological, immunohistochemical, and Real-Time PCR techniques

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Δημοσιευμένα: Jan 18, 2024
Ενημερώθηκε: 2024-01-18
DOI: https://doi.org/10.12681/jhvms.34093
Sezgin Deniz
Istanbul University, Institute of Health Sciences, İstanbul, Türkiye
Ahmet Gülçubuk
Istanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Pathology, İstanbul, Türkiye
Kıvılcım Sönmez
Istanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Pathology, İstanbul, Türkiye
Eda Altan
Istanbul University, Institute of Health Sciences, İstanbul, Türkiye
Işıl Aytemiz Danyer
Istanbul University-Cerrahpasa, Institute of Graduate Studies, Istanbul, Türkiye
Aydın Gürel
Istanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Pathology, İstanbul, Türkiye
Hüseyin Yılmaz
Istanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Virology, İstanbul, Türkiye
Περίληψη

Equine herpesvirus-1 (EHV-1) is a cause of pneumonia, encephalitis, and abortion in horses. Abortions occur alive or dead in the last trimester of pregnancy. The aim of this study is to investigate the incidence of EHV-1 infections in the tissue samples of aborted stillborn and live-born foals and the foals which died soon after birth and compare and correlate the conventional histopathologic diagnosis with IHC and Real-Time PCR. For this purpose, tissue samples (lung, liver, spleen, kidney, placenta, and brain) of 38 foals from Istanbul, Kocaeli, Bursa, and Eskişehir provinces of Turkey between August 2009 and March 2011 were examined Real-Time PCR revealed 52.6 % positivity (20/38), whereas 39.4 % (15/38) positivity was achieved with IHC, which indicated a correlation between these two diagnostic methods. In histopathological evaluation, the inclusion body was found in at least one of the tissue sections belonging to the lung, spleen, and mostly liver of 20 foals (52.6%).  Tissue samples of the foals found to be positive by IHC and PCR revealed histopathologic findings compatible with the characteristics of the disease.

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Αναφορές
Alibaşoğlu M (1969) Kısrakların viral abortusunda görülen patolojik bulgular ve bazı epizootiolojik gözlemler üzerinde araştırmalar. Ankara
Univ Vet Fak Derg 16(4):324-336.
Correa WM (1970) A Rapid Method for the Diagnosis of Equine Virus
Abortion. Can. j. comp. med 34(2): 164-166.
Diallo IS, Hewitson G, Wright L, Rodwell BJ, Corney BG (2006) Detection of equine herpesvirus type 1 using a real-time polymerase chain
reaction. J. Virol. Methods 131:92-98.
Eaglesome MD, Garcia MM (1979) Contagious equine metritis: A review.
Can Vet J 20: 201-206.
Edington N, Smith B, Griffiths L (1991) The role of endothelial cell infection in the endometrium, placenta and fetus of equid herpesvirus 1
(EHV-1) abortions. J Comp Pathol 104: 379-387.
Erer H, Kıran MM, Çiftçi K (2000) Veteriner Genel Patolji. Bahçıvanlar
Basım Sanayi A.Ş., Konya: pp 267-276.
Galosi CM, Vila Roza MV, Olivia GA, Pecorado MR, Echeverria MG,
Corva S, Etcheverrigaray ME (2001) A polymerase chain reaction for
detection of equine herpesvirus-1 in routine diagnostic submissions
of tissues from aborted foetuses. J Vet Med B Infect Dis Vet Public
Health 48:341-346.
Gerst S, Borchers K, Gower SM, Smith KC (2010) Detection of EHV-1
and EHV-4 in placental sections of naturally occurring EHV-1- and
EHV-4-related abortions in the UK: use of the placenta in diagnosis.
Equine Vet J 35(5):430-433.
Gilkerson RJ, Whalley MJ, Drummer EH, Studdert JM, Love ND (1999)
Epidemiological studies of equine herpesvirus 1 (EHV-1) in thorougbred foals: a review of studies conducted in the Hunter Valley of New
South Wales between 1995 and 1997. Vet Microbiol 68:15-25.
Hazıroğlu R, Milli ÜH (2001) Veteriner Patoloji. 2nd volume. Özkan Matbaacılık, Ankara: pp 527-528.
Kydd JH, Smith KC, Hannant D, Livesay GJ, Mumford JA (1994a) Distribution of equid herpesvirus-1 in the respiratory tract of ponies: implications for vaccination strategies. Equine Vet J 26:466-9.
Kydd JH, Smith KC, Hannant D, Livesay GJ, Mumford JA (1994b) Distribution of equid herpesvirus-1 in the respiratory tract-associated
lymphoid tissue: implications for cellular immunity. Equine Vet J
:470-473.
Léon A, Fortier G, Fortier C, Freymuth F, Trappest J, Leclercq R, Pronost
S (2008) Detection of equine herpesviruses in aborted foetuses by
consensus PCR. Vet Microbiol 126:20-29.
Mackie JT, Macleod GA, Reubel GH, Studdert MJ (1996) Diagnosis of
equine herpesvirus 1 abortion using polymerase chain reaction. Aust
Vet J 74(5):390-391.
Mukaiya R, Kimura T, Ochiai K, Wada R, Umemura T (2000) Demonstration of equine herpesvirus-1 gene expression in the placental trophoblasts of naturally aborted equine fetuses. J Comp Pathol 123:119-
Murray MJ, Del Piero F, Jeffrey SC (1998) Neonatal equine herpesvirus
type 1 infection on thoroughbred breeding farm. J Vet Intern Med
:36-41.
Oladunni FS, Horohov DW, Chambers TM (2019) EHV-1: A constant
threat to the horse industry. Front Microbiol 10:2668.
Özdamar K (1999) SPSS ile Biyoistatistik. 3rd ed, Kaan kitabevi, Eskişehir: pp 411-414.
Pamukçu M (1975) Veteriner Patoloji Dişi Üreme Organlarının
Hastalıkları. 3rd volume. A.Ü. Basımevi, Ankara.
Patel JR, Edington N, Mumford JA (1982) Variation in cellular tropism
between isolates of equine herpesvirus 1 in foals. Arch Virol 74: 41-
Rimstad E, Evensen Ø (1993) The identification of equid herpesvirus 1 in
paraffin-embedded tissues from aborted fetuses by polymerase chain
reaction and immunohistochemistry. J Vet Diagn Invest 5:174-183.
Schultheiss PC, Collins JK, Carnman J (1993) Use of an immunoperoxidase technique to detect equine herpesvirus-1 in formalin-fixed parafin-embedded equine fetal tissues. J Vet Diagn Invest 5:12-15.
Siedek ME, Whelan M, Edington N, Hamblin A (1999) Equine herpesvirus type 1 infects dendritic cells in vitro: stimulation of T lymphocyte
proliferation and cytotoxicity by infected dendritic cells. Vet Immunol Immunopathol 67:177-32.
Smith KC, Mumford JA, Lakhani K (1996) A comparison of equid herpesvirus-1 (EHV-1) vascular lesions in the early versus late pregnant
equine uterus. J Comp Pathol 114:231-246.
Smith DJ, Hamblin AS, Edington N (2001) Infection of endothelial cells
with Equine herpesvirus-1 (EHV-1) occurs where there is activation
of putative adhesion molecules: a mechanism for transfer of virus.
Equine Vet J 33:138-142.
Sugahara Y, Matsumura T, Kono Y, Honda E, Kida H, Okazaki K (1997)
Adaptation of equine herpesvirus 1 to unnatural host led to mutation
of the GC resulting increased susceptibility of the virus to heparin.
Arch Virol 142:1849-56.
Szeredi L, Aupperle H, Steiger K (2003) Detection of equine herpesvirus-1 in the fetal membranes of aborted equine fetuses by immunohistochemical and In-situ hybridization techniques. J Comp Pathol
:147-153.
Taouji S, Collobert C, Gicquel B, Sailleau C, Brisseau N, Moussu C,
Breuil FM, Pronost, S, Borchers K, Zientara S (2002) Detection and
isolation of equine herpesvirus 1 and 4 from horses in Normandy: an
Autopsy study of tissue distrubution in relation to vaccination status.
J Vet Med B Infect Dis Vet Public Health 49:394-399.
Taylor CR, Shi S-R, Barr NJ, Wu N (2006) Techniques of immunohistochemistry: principles, pitfalls, and standardization. In: Diagnostic
Immunohistochemistry. 2nd ed, Churchill Livingstone, New York: pp
-43.
Yeşildere T, Gürel A, Arun SS (1994) Türkiye’de İzmit yöresinden özel
haralardaki kısraklarda Rhinopneumonitis Equi’nin patolojisi üzerine
çalışmalar. Istanbul Univ Vet Fak Derg 20(1):151-160.
Zhang YF, Smith P, Tarbet ED, Osterrieder N, Jennings S, O’challaghan
DJ (1998) Protective immunity against equine herpesvirus type-1
(EHV-1) infection in mice induced by recombinant EHV-1 gD. Virus
Res 56:11-24.