Ascorbic acid enhances the treatment efficacy of tetracycline against experimentally infected Aeromonas hydrophila
Περίληψη
Combinations of two or more pharmaceuticals are sometimes used to achieve efficient treatment of an ailment. The objectives of this study were to evaluate the synergistic action of ascorbic acid combined with tetracycline in the treatment of Aeromonas hydrophila infection. A total of 210 apparently healthy 2-month-old uniformly sized Clarias gariepinus with a mean weight of 24.65±2.78 g were used in the study. The fish were randomly assigned to seven treatment groups (A, B, C, D, E, F and G), each in triplicate. The fish in group A were the uninfected controls, while those in groups B to G were infected with the bacterium and were either treated or not treated with tetracycline, and their diets were either supplemented with ascorbic acid or not. Growth, survival, hematological parameters, serum chemistry responses were investigated as bioindicators of health status. Infected fish deprived of L-ascorbic acid presented lower growth and survival rates. Hemorrhages, skin and fin erosions and skeletal deformities were some of the lesions observed. There were significantly (P<0.05) lower red blood cell counts and hemoglobin; significantly higher (P<0.05) total leucocyte counts; total protein, globulin and albumin; and elevated A/G ratios, also significantly higher alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and urea levels when compared with the group without ascorbic acid supplementation. This study suggested that ascorbic acid supplementation at 100 and 200 mg/kg is recommended in the diet of C. gariepinus to potentiate the action of tetracycline in the treatment of A. hydrophila infection.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
-
Omeje, V., Okolo, C., Kolndadacha, O., & Ezema, C. (2025). Ascorbic acid enhances the treatment efficacy of tetracycline against experimentally infected Aeromonas hydrophila. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 76(3), 9553–9564. https://doi.org/10.12681/jhvms.39047
- Τεύχος
- Τόμ. 76 Αρ. 3 (2025)
- Ενότητα
- Research Articles
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Λήψεις
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Αναφορές
Adewolu MA, Aro OO. (2009). Growth, feed utilization and haematology
of Clarias gariepinus (Burchell, 1822) fingerlings fed diets
containing different levels of vitamin C. American Journal of Applied
Sciences, 6(9), 1675.
Adham KG, Hashem HO, Abu-Shabana MB, Kamel AH (2000). Vitamin
C deficiency in the Catfish Clarias gariepinus, Aquaculture Nutrition,
Affonso EG Polez VLP, Corrêa CF, Mazon AF, Araújo MRR, Moraes
G, Rantin FT (2002). Blood parameters and metabolites in the
teleost fish Colossoma macropomum exposed to sulfide or hypoxia,
Comparative Biochemistry & Physiology, 133, 375–382. https://
doi.org/10.1016/S1532-0456(02)00127-8
Akter R, Pervin MA, Jahan H, Rakhi SF, Mohsinul Reza AHM, Hossain
Z (2020). Toxic effects of an organophosphate pesticide, envoy
SC on the histopathological, hematological, and brain acetylcholinesterase
activities in stinging catfish (Heteropneustes fossilis),
Journal of Basic and Applied Zoology, 81, 1-14. https://doi.
org/10.1186/s41936-020-00184-w
Ben Y, Fu C, Hu M, Liu L, Wong MH, Zheng C (2019). Human health
risk assessment of antibiotic resistance associated with antibiotic
residues in the environment: A review, Environmental Research,
, 483-493. https://doi.org/10.1016/j.envres.2018.11.040
Bose A (2006). Aeromonas and Plesiomonas spp. Principles and Practice
of Clinical Bacteriology, 419-426.
Chaijan M, Undeland, I (2015). Development of a new method for determination
of total haem protein in fish muscle. Food Chemistry, 173,
Cheesebrough M (2002). District Laboratory Practice in Tropical Countries,
nd ed. (Cambridge University Press, Cambridge).
Chenia HY, Duma S (2017). Characterization of virulence, cell surface
characteristics and biofilm-forming ability of Aeromonas spp. isolates
from fish and sea water, Journal of Fish Diseases, 40, 339–350.
Chernyavskikh SD, Borodaeva ZA, Borisovskiy IP, Ostapenko SI,
Galtseva OA (2019). Blood protein spectrum in representatives of
the fish superclass, EurAsia Journal of Biosciences 13, 979-981.
bloodstream (Muthusamy et al., 2018). The decreases
in the levels of AST and ALT among the groups
whose diets were supplemented with ascorbic acid
compared with those whose diets were not supplemented
with ascorbic acid obtained in this study
indicate hepato-protective activity and a reduction
in organ damage resulting from ascorbic acid supplementation.
Elevated levels of creatinine and urea
are a consequence of damage to the kidney, leading
to the inability of the excretory organs to eliminate
them from the blood circulation. In fish, urea is produced
by the liver and excreted mainly through the
gills. Toxicants and pathogens that cause gill damage
and dysfunction ultimately predispose individuals to
elevated urea. Additionally, renal damage induced
by the exposure of fish to toxicants and pathogens
can lead to elevated creatinine, which is used as a
kidney function test.
The results of this study revealed that total protein,
albumin and globulin increased significantly
in fish fed a diet supplemented with ascorbic acid.
These findings may indicate immune system stimulation
and a stronger innate immune response. Conversely,
the hypoproteinaemia and hypoalbuminemia
recorded among the group deprived of ascorbic acid
can be attributed to decreased food intake, conversion
and utilization or even liver and kidney damage.
The importance of globulin in maintaining a healthy
immune system has been reported (Jagruthi et al.,
. All blood proteins necessary for immune responses
are products of gamma globulin. The stimulatory
effects of ascorbic acid on the immune system
have been reported in other fish species, such as
Caspian brown trout (Khara et al., 2016), Catla catla
(Reddy, 2018), and common carp (Faramarzi, 2012).
Like other vertebrates, albumin, whose main function
is the transport of nutrients, metabolites and xenobiotics,
is the most abundant protein in the blood
plasma of fish (Chernyavskikh et al., 2019). In this
study, the differences in the albumin concentration
between the treatment groups were not significant,
which showed that the diet did not predispose the
treated fish to osmoregulatory dysfunction.
In conclusion, the results of the present study
suggested that ascorbic acid supplementation might
be useful in ameliorating the deleterious effects of
A. hydrophila infection on RBC, hemoglobin, PCV,
and WBC counts and serum biochemical parameters.
Also the results of the study shows that ascorbic acid
supplementation enhanced the treatment potential
of tetracycline against A. hydrophila infection of
C. gariepinus. Furthermore, there were no adverse
effects of ascorbic acid supplementation on the different
parameters evaluated.
Acknowledgments
The authors express their appreciation to the Nigerian
Tertiary Education Trust Fund (TETFUND)
for providing funding via the Institution-Based Research
Intervention (TETFUND/DESS/UNI/NSUKKA/
/RP/VOL.1) for the present study.
Conflict of interest
The authors have no relevant financial or non-financial
interests to disclose.
Dias MKR, Sampaio LS, Proietti-Junior AA, Yoshioka ETO, Rodrigues
DP (2016). Lethal dose and clinical signs of Aeromonas hydrophila
in Arapaima gigas (Arapaimidae), the giant fish from Amazon,
Veterinary Microbiology, 188: 12–15.
Docan A, Grecu I, Dediu L (2018). Use of hematological parameters
as assessment tools in fish health status. Journal of Agrolimentary
Processes and Technologies, 24, 317-324.
Dunham R, Elaswad A (2018). Catfish biology and farming. Annual
Review of Animal Biosciences, 6, 305–325. https://doi.org/10.1146/
annurev-animal-030117-014646
ELbialy ZI, Atef E, Al-Hawary I I, Salah AS, Aboshosha AA, Abualreesh
MH, Assar DH (2023). Myostatin-mediated regulation of skeletal
muscle damage post-acute Aeromonas hydrophila infection in Nile
tilapia (Oreochromis niloticus L.). Fish Physiology and Biochemistry,
(1), 1-17.
Eya JC (1996). `Broken-skull disease’ in African catfish Clarias
gariepinus is related to a dietary deficiency of ascorbic acid,
Journal of world aquaculture society, 27: 493-498. https://doi.
org/10.1111/j.1749-7345.1996.tb00635.x
Faramarzi M (2012). Effect of dietary vitamin C on growth and feeding
parameters, carcass composition and survival rate of Common Carp
(Cyprinus carpio), Global Veterinarian, 8, 507-510.
Froese R (2006). Cube law, condition factor and weight–length relationships:
history, meta‐analysis and recommendations. Journal of
applied ichthyology, 22(4), 241-253. https://doi.org/10.1111/j.1439-
2006.00805.x
Garcia Moreira V, Beridze Vaktangova N, Martinez Gago MD, Laborda
Gonzalez B, Garcia Alonso S, Fernandez Rodriguez E (2018) Overestimation
of albumin measured by bromocresol green vs bromocresol
purple method: influence of acute-phase globulins. Laboratory
medicine. 49(4):355-61. https://doi.org/10.1093/labmed/lmy020
Gharaei A, Shafiei M, MirdarHarijani J, Hassanein P, Arshadi A (2020).
Immune Responses and Hematological Parameters Changes of
Rainbow Trout (Oncorhynchus mykiss) under Effects of Dietary
Administration of Sumac (RhuscoriariaL.), Journal of Agricultural
Science and Technology, 22: 173-186.
Hamid NH, Hassan MD, Sabri MYM, Hasliza AH, Hamdan RH, Afifah
MNF, Raina MS, Nadia ABS, Fuad MM (2017). Studies on Pathogenicity
effect of Aeromonas hydrophila infection in juvenile red
hybrid tilapia Oreochromis sp., Proceedings of International Seminar
on Livestock Production and Veterinary Technology, 532–539.
Harikrishnan R, Nisha Rani M, Balasundaram C (2003). Hematological
and biochemical parameters in common carp, Cyprinus carpio, following
herbal treatment for Aeromonas hydrophila infection, Aquaculture
Huang XJ, Choi YK, Im HS, Yarimaga O, Yoon E, Kim HS (2006). Aspartate
aminotransferase (AST/GOT) and alanine aminotransferase
(ALT/GPT) detection techniques. Sensors, 6(7), 756-782. https://
doi.org/10.3390/s6070756
Ibiyo LMO, Atteh JO, Omotosho JS, Madu, CT (2007). Vitamin C
(Ascorbic acid) requirements of Heterobranchus longifilis fingerlings,
African Journal of Biotechnology, 6, 1559-1567.
Jagruthi C Yogeshwari G, Anbazahan SM, Mari LSS, Arockiaraj J
Mariappan P, Sudhakar GRL, Balasundaram C, Harikrishnan R
(2014). Effect of dietary astaxanthin against Aeromonas hydrophila
infection in common carp, Cyprinus carpio, Fish & Shellfish
Immunology, 41, 674-680. https://doi.org/10.1016/j.fsi.2014.10.010
Julinta RB, Roy A, Singha J, Abraham TJ, Patil PK (2017). Evaluation
of efficacy of oxytetracycline oral and bath therapies in Nile tilapia,
Oreochromis niloticus against Aeromonas hydrophila infection,
International Journal of Current Microbiology Applied Science,
, 62-76.
Khara H, Sayyadborani M, Sayyadborani, M (2016). Effects of α-Tocopherol
(vitamin E) and Ascorbic acid (vitamin C) and their
combination on growth, survival and some hematological and
immunological parameters of Caspian brown trout, Salmo Trutta
Caspius juveniles, Turkish Journal Fisheries & Aquatic Sciences,
, 385-393. https://doi.org/10.4194/1303-2712-v16_2_18
Li T, Lu J, Wu J, Zhang Z, Chen L (2022). Predicting aquaculture water
quality using machine learning approaches. Water, 14(18), 2836.
Lundström SV, Östman M, Bengtsson-Palme J, Rutgersson C, Thoudal
M, Sircar T, Blanck H, Eriksson KM, Tysklind M, Flach CF, Larsson
DJ (2016). Minimal selective concentrations of tetracycline in
complex aquatic bacterial biofilms. Science of the Total Environment,
Muthusamy N, Vasantharaja D, Ramalingam V (2018). Protective Effect
of Propolis on Hematological and Biochemical Changes in Fresh
Water Fish Cirrhinus mrigala Exposed to Pyrethroid Pesticide Fenvalerate,
Journal of Biological and Chemical Research 35, 883-890.
Okhionkpamwonyi ON, Edema CU (2017). Effects of Supplemental
Vitamin C (Ascorbic Acid) on the Growth and Health of African
Catfish Clarias gariepinus, Journal of Applied Science and Environmental
Management, 21, 177-183. https://doi.org/10.4314/
jasem.v21i1.18
Oluyemi GK (2021). Effects of ascorbic acid and iron nanoparticles
supplements on hyperthermia-induced stress in African catfish,
Clarias gariepinus (Burchell, 1822), The Journal of Basic and Applied
Zoology, 82, 1-9. https://doi.org/10.1186/s41936-021-00256-5
Omeje VO, Okolo CC, Eze DC (2019). Pathogenicity (LD50) and antibiotics
sensitivity tests of Aeromonas hydrophila isolated from
fishes of the Kainji Lake area, International Journal of Fisheries
and Aquatic Studies, 7, 190-196.
Peterman MA, Posadas BC (2019). Direct Economic Impact of Fish
Diseases on the East Mississippi Catfish Industry, North American
Journal of Aquaculture, 81, 22–229. https://doi.org/10.1002/
naaq.10090
Rafiq M, Sarder I, Thompson KD, Penman DJ, McAndrew BJ (2001).
Immune responses of Nile tilapia (Oreochromis niloticus L.) clones:
I. Non specific responses, Development & Comparative Immunology,
, 37-46.
Reddy SJ (2018). Immunostimulatory Effect of Supplementary Diet
Vitamin C on Growth, Hematology, Survival and Immunity of Fish,
Catla catla, IOSR Journal of Pharmacy, 8, 46-54.
Reyes AT, Aliasas, NC (2018). White Blood Cell Response of Nile
Tilapia (Oreochromis Niloticus L.) to Single, Double and Multiple
Bacterial Infections, Advanced Pharmacology &Clinical Trials, 3,
Rodger HD (2016). Fish disease causing economic impact in global
aquaculture. In: A. Adams, editor. Fish vaccines: Birkhäu-ser advances
in infectious diseases. Springer, Basel, Switzerland, 1–34.
Sarkar MJA, Rashid MM (2012). Pathogenicity of the bacterial isolates
Aeromonas hydrophila to catfishes, carps and perch, Journal
of Fisheries, 44, 393-
Semwal A, Kumar A, Kumar N (2023). A review on pathogenicity of
Aeromonas hydrophila and their mitigation through medicinal
herbs in aquaculture. Heliyon, 9(3). https://doi.org/10.1016/j.heliyon.
e14088
Song Y Hu X, Lü A, Sun J, Yiksung Y, Pei C, Zhang C, Li L (2017). Isolation
and characterization of Aeromonas veronii from ornamental fish
species in China. Israeli Journal of Aquaculture-Bamidgeh, 69(1).
Stratev D, Odeyemi OA (2017). An overview of motile Aeromonas septicaemia
management, Aquaculture International, 25,1095–1105.
Stoskopf MK (1993). Clinical pathology in fish medicine. (W. B. Saunders
Company, Hartcourt Brace Jovanourah Inc.)
Tran-duy A, Smit B, Dam AAV, Schrama J (2008). Effects of dietary
starch and energy levels on maximum feed intake , growth and
metabolism of Nile tilapia , Oreochromis niloticus, Aquaculture,
Trichet V, Santigosa E, Cochin E, Gabauden J (2015). The effect of
vitamin c on fish health, Dietary Nutrients, Additives, and Fish
Health, 2015, 151-171. https://doi.org/10.1002/9781119005568.ch7
Tulaby Dezfuly Z Alishahi M, Ghorbanpoor M, Tabandeh MR, Mesbah
M (2019). Effects of Lipopolysaccharides (LPS) of Yersinia ruckeri
on Immune Response in Rainbow Trout (Oncorhynchus mykiss) by
Intraperitoneal and Oral Administration, Iranian Journal Veterinary
Medicine, 13, 421-436. https://doi.org/ijvm.2019.275717.1004969
Weyl OLF, Daga VS, Ellender BR, Vitule JRS (2016). A review of
Clarias gariepinus invasions in Brazil and South Africa, Journal
Fish Biology, 89, 386 – 402. https://doi.org/10.1111/jfb.12958
Witeska M, Kondera E, Ługowska K, Bojarski B (2022). Hematological
methods in fish–Not only for beginners. Aquaculture, 547, 737498.
Youssef BBA, Soror EI, Abo Kora SY, Atwa SAE, Hamouda AF (2021). Protective Role of Vitamin C and Thyme Extract (Thymus vulgaris)
on Chromium-Induced Toxicity in Catfish (Clarias gariepinus),
Asian Journal Animal & Veterinary Advances, 16, 1-13. https://
doi.org/10.3923/ajava.2021.1.13
Zhang D, Xu DH, Shoemaker C (2016). Experimental induction of
motile Aeromonas septicemia in channel catfish (Ictalurus punctatus)
by waterborne challenge with virulent Aeromonas hydrophila,
Aquaculture Report, 3, 18 – 23. https://doi.org/10.1016/j.
aqrep.2015.11.003Get
