Probiotic Characteristics of Enterocin-Producing Enterococci Isolated from Traditional Turkish Cheeses
Περίληψη
This study aimed to evaluate the probiotic properties of the bacteriocin-producing strains E. faecium DP8.3, E. faecium DP9.3 and E. mundtii DP35.1. All strains were found to survive at pH 3.0 and maintained their viability in the presence of 0.4% phenol and 100 ppm lysozyme. In simulated gastric juice adjusted to pH 3.0, the viable cell counts for E. faecium DP8.3, DP9.3, and E. mundtii DP35.1 were 8.96±0.01, 8.37±0.00, and 8.63±0.21, respectively. The bacteriocin-producing strains were capable of surviving at bile salt concentrations of 0.3%, 0.5%, and 1%. Additionally, these strains were able to grow in the presence of taurocholic acid, glycocholic acid, taurodeoxylic acid sodium salts, and glycodeoxycholic acid sodium salts. The strains exhibited high hydrophobicity, autoaggregation, and coaggregation abilities. According to the results, these bacteriocin-producing strains possess probiotic properties, suggesting their potential use as protective probiotic cultures in food production. Further studies, including in vivo studies and food trials, is required to assess the potential of these strains as protective probiotic cultures in the food industry.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
-
Kankaya, D. (2025). Probiotic Characteristics of Enterocin-Producing Enterococci Isolated from Traditional Turkish Cheeses. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 76(3), 9701–9710. https://doi.org/10.12681/jhvms.39888
- Τεύχος
- Τόμ. 76 Αρ. 3 (2025)
- Ενότητα
- Research Articles
Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.
Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.
Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.
Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc
Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Ahl D, Liu H, Schreiber O, Roos S, Phillipson M, Holm L (2016)
Lactobacillus reuteri increases mucus thickness and ameliorates
dextran sulphate sodium-induced colitis in mice. Acta Physiol
(4):300-310.
Ahmadova A, Todorov SD, Choiset Y, Rabesona H, Zadi TM, Kuliyev
A, de Melo Franco BDG, Chobert JM, Haertlé T (2013) Evaluation
of antimicrobial activity, probiotic properties and safety of wild
strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal
cheese. Food Control 30(2):631-641.
Akpınar Kankaya D (2024) Technological and safety properties of bacteriocin-
producing Enterococcus strains isolated from traditional
Turkish cheeses. Qual Assur Saf Crop 16(4):40-56.
Anjana A, Tiwari SK (2022) Bacteriocin-producing probiotic lactic
acid bacteria in controlling dysbiosis of the gut microbiota. Front
Cell Infect Mi 12:851140.
Ayyash M, Abushelaibi A, Al-Mahadin S, Enan M, El-Tarabily K, Shah
N (2018) In-vitro investigation into probiotic characterisation of
Streptococcus and Enterococcus isolated from camel milk. LWTFood
Sci Technol 87:478-487.
Bagci U, Ozmen Togay S, Temiz A, Ay M (2019) Probiotic characteristics
of bacteriocin-producing Enterococcus faecium strains isolated
from human milk and colostrum. Folia Microbiol 64:735-750.
Basson A, Flemming LA, Chenia HY (2008) Evaluation of adherence,
hydrophobicity, aggregation characteristics and biofilm development
of Flavobacterium johnsoniae-like isolates from South African
aquaculture systems. Microbial Ecol 55:1-14.
Benyacoub J, Cavadini C, Sauthier T, Schiffrin EJ, von der Weid T,
Czarnecki-Maulden GL, Anderson RE (2003) Supplementation of
food with Enterococcus faecium (SF68) stimulates immune functions
in young dogs. J Nutr 133(4):1158-1162.
Binda S, Hill C, Johansen E, Obis D, Pot B, Sanders ME, Tremblay A,
Ouwehand AC (2020) Criteria to qualify microorganisms as “probiotic”
in foods and dietary supplements. Front Microbiol 11:1662.
Brennan M, Wansmail B, Johnson BC, Ray B (1986) Cellular damage
in dried Lactobacillus acidophilus. J Food Protect 49(1):47-53.
Chakchouk-Mtibaa A, Smaoui S, Ktari N, Sellem I, Najah S, Karray-Rebai
I, Mellouli L (2017) Biopreservative efficacy of bacteriocin
BacFL31 in raw ground Turkey meat in terms of microbiological,
physicochemical, and sensory qualities. Biocontrol Sci 22(2):67-77.
Chou LS, Weimer B (1999) Isolation and characterization of acid- and
bile-tolerant isolates from strains of Lactobacillus acidophilus. J
Dairy Sci 82:23-31.
Conway PL, Gorbach SL, Goldin BR (1987) Survival of lactic acid
bacteria in the human stomach and adhesion to intestinal cells. J
Dairy Sci 70(1):1-12.
Corzo G, Gilliland SE (1999) Bile salt hydrolase activity of three strains
of Lactobacillus acidophilus. J Dairy Sci 82(3):472-480.
Çetin B, Aktaş H (2024) Monitoring probiotic properties and safety evaluation
of antilisterial Enterococcus faecium strains with cholesterol-
lowering potential from raw Cow’s milk. Food Biosci 61:104532.
Daba GM, El-Dien AN, Saleh SAA, Elkhateeb WA, Awad G, Nomiyama
T, Yamashiro K, Zendo T (2021) Evaluation of Enterococcus strains
newly isolated from Egyptian sources for bacteriocin production and
probiotic potential. Biocatal Agric Biotechnol 35:102058.
Dapkevicius MLE, Sgardioli B, Câmara SPA, Poeta P, Malcata, FX
(2021) Current trends of enterococci in dairy products: a comprehensive
review of their multiple roles. Foods 10(4):821.
Dressman JB, Berardi RR, Dermentzoglou LC, Russell TL, Schmaltz
SP, Barnett JL, Jarvenpaa KM (1990) Upper gastrointestinal (GI)
pH in young, healthy men and women. Pharm Res 7(7):756-761.
Favaro L, Basaglia M, Casella S, Hue I, Dousset X, de Melo Franco
BDG, Todorov SD (2014) Bacteriocinogenic potential and safety
evaluation of non-starter Enterococcus faecium strains isolated
from homemade white brine cheese. Food Microbiol 38:228-239.
Food and Agriculture Organization of the United Nations and World
Health Organization (FAO/WHO) (2002) Joint FAO/WHO Working
Group Report on Drafting Guidelines for the Evaluation of Probiotics
in Food, pp. 1-11. London, Ontario, Canada.
Foulquié Moreno MR, Sarantinopoulos P, Tsakalidou E, De Vuyst L
(2006) The role and application of enterococci in food and health.
Int J Food Microbiol 106(1): 1-24.
Gilliland SE, Walker DK (1990) Factors to consider when selecting a
culture of Lactobacillus acidophilus as a dietary adjunct to produce
a hypocholesterolemic effect in humans. J Dairy Sci 73(4):905-911.
Graham K, Stack H, Rea R (2020) Safety, beneficial and technological
properties of enterococci for use in functional food applications-a
review. Crit Rev Food Sci 60(22):3836-3861.
Hossain MI, Sadekuzzaman M, Ha SD (2017) Probiotics as potential
alternative biocontrol agents in the agriculture and food industries:
a review. Food Res Int 100:63-73.
Khaneghah AM, Abhari K, Eş I, Soares MB, Oliveira RBA, Hosseini
H, Rezaei M, Balthazar CF, Silva R, Cruz AG, Ranaheera CS,
Sant’Ana AS (2020) Interactions between probiotics and pathogenic
microorganisms in hosts and foods: a review. Trends Food
Sci Tech 95:205-218.
Kobyliak N, Conte C, Cammarota G, Haley AP, Styriak I, Gaspar L,
Fusek J, Rodrigo L, Kruzliak P (2016) Probiotics in prevention and
treatment of obesity: a critical view. Nutr Metab 13:1-13.
Krawczyk B, Wityk P, Gałęcka M, Michalik M (2021) The many faces
of Enterococcus spp.-commensal, probiotic and opportunistic
pathogen. Microorganisms 9:1900.
Kristich CJ, Rice LB, Arias CA (2014) Enterococcal infection: treatment
and antibiotic resistance. In: Enterococci:From Commensals to
Leading Causes of Drug Resistant Infection. Massachusetts Eye and
Ear Infirmary, Boston, www.ncbi.nlm.nih.gov/books/NBK190424/.
Kumar Y, Singh L (2009) Health benefits of fermented and functional
foods. J Plant Dev Sci 1:151-155.
Lau CS, Chamberlain RS (2016) Probiotics are effective at preventing
Clostridium difficile-associated diarrhea: a systematic review and
meta-analysis. Int J Gen Med 9:27-37.
Nami Y, Bakhshayesh RV, Jalaly HM, Lotfi H, Eslami S, Hejazi MA
(2019) Probiotic properties of Enterococcus isolated from artisanal
dairy products. Front Microbiol 10:300.
Nascimento LCS, Casarotti SN, Todorov SD, Penna ALB (2019) Probiotic
potential and safety of enterococci strains. Ann Microbiol
:241-252.
Olajugbagbe TE, Elugbadebo OE, Omafuvbe BO (2020) Probiotic potentials
of Pediococuss acidilactici isolated from wara; A Nigerian
unripened soft cheese. Heliyon 6(9):e04889.
Orihuel A, Bonacina J, Vildoza MJ, Bru E, Vignolo G, Saavedra L,
Fadda S (2018) Biocontrol of Listeria monocytogenes in a meat
model using a combination of a bacteriocinogenic strain with curing
additives. Food Res Int 107:289-296.
Öztürk H, Geniş B, Özden Tuncer B, Tuncer Y (2024) In vitro probiotic
properties of enterocin-producing Enterococcus mundtii and Enterococcus
faecium strains isolated from sheep and goat colostrum.
J Hellenic Vet Med Soc 75(1):7099-7110.
Pajarillo EA, Chae JP, Balolong MP, Kim HB, Park CS, Kang DK
(2015) Effects of probiotic Enterococcus faecium NCIMB 11181
administration on swine fecal microbiota diversity and composition
using barcoded pyrosequencing. Anim Feed Sci Technol 201:80-88.
Rajput K, Dubey RC, Kumar A (2022). Probiotic potential and immunomodulatory
properties in Enterococcus faecium GMB24 and Enterococcus
hirae SMB16 isolated from goat and sheep milk. Arch
Microbiol 204(10):619.
Santos KMOD, Vieira ADS, Salles HO, Oliveira JdS, Rocha CRC, de
Borges MF, Bruno LM, de Franco BDGM (2015) Safety, beneficial
and technological properties of Enterococcus faecium isolated from
Brazilian cheeses. Braz J Microbiol 46(1):237-49.
Sarkar SL, Hossain MI, Monika SA, Sanyal SK, Roy PC, Hossain MA,
Jahid IK (2020) Probiotic potential of Pediococcus acidilactici and
Enterococcus faecium isolated from indigenous yogurt and raw goat
milk. Microbiol Biotechnol Lett 48(3):276-286.
Sheikhi A, Shakerian M, Giti H, Baghaeifar M, Jafarzadeh A, Ghaed V,
Heibor MR, Baharifar N, Dadafarin Z, Bashirpour G (2016) Probiotic
yogurt culture Bifidobacterium animalis subsp. lactis BB-12 and
Lactobacillus acidophilus LA-5 modulate the cytokine secretion by
peripheral blood mononuclear cells from patients with ulcerative
colitis. Drug Res 66:300-305.
Shi Y, Zhai M, Li J, Li B (2020) Evaluation of safety and probiotic
properties of a strain of Enterococcus faecium isolated from chicken
bile. J Food Sci Technol 57(2):578-587.
Son SH, Yang SJ, Jeon HL, Yu HS, Lee NK, Park YS, Paik HD (2018)
Antioxidant and immunostimulatory effect of potential probiotic
Lactobacillus paraplantarum SC61 isolated from Korean traditional
fermented food, jangajji. Microb Pathogenesis 125:486-492.
Teply M (1984) Cistemlekarskekultury. Phara. SNTL Nakladatelstvi.
TechnickeLitertury. In: Starters for Fermented Milks, Kurmann JA
(Ed), IDF Bulletin, 227:41-55.
Terzić-Vidojević A, Veljović K, Popović N, Tolinački M, Golić N (2021)
Enterococci from raw-milk cheeses: current knowledge on safety,
technological, and probiotic concerns. Foods 10:2753.
Toplu MS, Özden Tuncer B (2023) Evaluation of the functional properties
and safety of enterocin-producing Enterococcus faecium
BT29.11 isolated from Turkish Beyaz Cheese and its inhibitory
activity against Listeria monocytogenes in UHT whole milk. Ital
J Food Sci 35(2):54-70.
Vinderola CG, Reinheimer JA (2003) Lactic acid starter and probiotic
bacteria: a comparative ‘‘in vitro’’study of probiotic characteristics
and biological barrier resistance. Food Res Int 36(9-10):895-904.
Wunderlich PF, Braun L, Fumagalli I, D’Apuzzo V, Heim F, Karly M,
Lodi R, Politta G, Vonbank F, Zeltner L (1989) Double-blind report
on the efficacy of lactic acid-producing Enterococcus SF68 in the
prevention of antibiotic-associated diarrhoea and in the treatment
of acute diarrhoea. J Int Med Res 17(4):333-338.
Yerlikaya O, Akbulut N (2020) In vitro characterisation of probiotic
properties of Enterococcus faecium and Enterococcus durans strains
isolated from raw milk and traditional dairy products. Int J Dairy
Technol 73(1):98-107.
Yıldırım N, Özden Tuncer B (2022) Determination of probiotic properties
of Pediococcus acidilactici and Pediococcus pentosaceus
strains with antimicrobial activity. Gıda 47(3):434-446.
Zommiti M, Chevalier S, Feuilloley MGJ, Connil N (2022) Special
Issue “Enterococci for Probiotic Use: Safety and Risk”: Editorial.
Microorganisms 10(3):604.
