Neopterin and Soluble Urokinase Type Plasminogen Activator Receptor as Biomarkers in Dogs with Systemic Inflammatory Response Syndrome

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DOI: https://doi.org/10.12681/jhvms.22936
Keywords:
Systemic inflammatory response syndrome acute phase proteins dogs
O. BASBUG
Department of Internal Medicine, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas, Turkey
U. AYDOGDU
Department of Internal Medicine, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey
Z. T, AGAOGLU
Department of Internal Medicine, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas, Turkey
Abstract

The systemic inflammatory response syndrome (SIRS) is a common cause of mortality in dogs. This study aims to investigate the diagnostic value of markers C-reactive protein (CRP), soluble urokinase-type plasminogen activator receptor (suPAR) and neopterin in dogs with SIRS. The materials of the study consist of 30 dogs with SIRS and 15 healthy dogs that show no clinical symptom, 45 dogs in total. CRP, suPAR and neopterin levels of the serum samples were determined by ELISA using commercial kits. In the study, it was found that CRP, suPAR and neopterin levels of dogs with SIRS were significantly higher (P < 0.001, P < 0.01 and P < 0.01, respectively) than those in the healthy group. The cut-off values for the healthy dogs and dogs with SIRS for CRP, suPAR and neopterin were determined to be 11.20 mg/L, 223.68 ng/L and 3.23 nmol/mL, respectively. At these cut-off values, sensitivity levels were determined as high (92.90%) for CRP, moderate (63.30%) for neopterin, and low (53.30%) for suPAR, and high specificities (93.30%) were determined for all. In conclusion, it was determined that CRP showed the best performance for the detection of systemic inflammatory response in dogs and neopterin and suPAR could be used as an alternative to CRP. It was also argued that further studies were necessary in order to determine diagnosis and prognosis of diseases.

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References
Alberti C, Brun-Buisson C, Goodman SV, Guidici D, Granton J, Moreno R, Le Gall JR (2003) Influence of systemic inflammatory response syndrome and sepsis on outcome of critically ill infected patients. Am J Respir Crit Care Med 168: 77-84.
Baydar T, Yuksel O, Sahin TT, Dikmen K, Girgin G, Sipahi H, Sare M (2009) Neopterin as a prognostic biomarker in intensive care unit patients. J Crit Care 24: 318-321.
Berdowska A, Zwirska-Korczala K (2001) Neopterin measurement in clinical diagnosis. J Clin Pharm Ther 26: 319-329.
Bilgili B, Cinel İ (2013) The significance of soluble urokinase plasminogen activator receptor (suPAR) in ICU patients. J Turk Soc Intens Care 11: 33-39 (in Turkish).
Brunkhorst FM, Eberhard OK, Brunkhorst R (1999) Discrimination of infectious and noninfectious causes of early acute respiratory distress syndrome by procalcitonin. Crit Care Med 27: 2172-2176.
Cesur S, Aslan T, Hoca NT, Çimen F, Tarhan G, Çifçi A, Şipit T (2014) Clinical importance of serum neopterin level in patients with pulmonary tuberculosis. Int J Mycobacteriol 3: 5-8.
Christensen MB, Langhorn R, Goddard A, Andreasen EB, Moldal E, Tvarijonaviciute A, Kjelgaard-Hansen M (2014) Comparison of serum amyloid A and C-reactive protein as diagnostic markers of systemic inflammation in dogs. Can Vet J 55: 161-168.
de Laforcade AM, Freeman LM, Shaw SP, Brooks MB, Rozanski EA, Rush JE (2003) Hemostatic changes in dogs with naturally occurring sepsis. J Vet Intern Med 17: 674-679.
Eckersall P, Conner J (1988) Bovine and canine acute phase proteins. Vet Res Commun 12: 169-178.
Ercan N, Tuzcu N, Başbug O, Tuzcu M, Alim A (2016) Diagnostic value of serum procalcitonin, neopterin, and gamma interferon in neonatal calves with septicemic colibacillosis. J Vet Diagn Invest 28: 180-183.
Eugen-Olsen J (2011) suPAR as a marker of disease severity and risk of mortality in sepsis. J Intern Med 270: 29-31.
Hoenigl M, Raggam RB, Wagner J, Valentin T, Leitner E, Seeber K, Zollner- Schwetz I, Krammer W, Prüller F, Grisold AJ, Krause R (2013) Diagnostic accuracy of soluble urokinase plasminogen activator receptor (suPAR) for prediction of bacteremia in patients with systemic inflammatory response syndrome. Clin Biochem 46:225-229.
Hoffmann G, Wirleitner B, Fuchs D (2003) Potential role of immune system activation-associated production of neopterin derivatives in humans. Inflamm Res 52: 313-321.
Holm JL, Rozanski EA, Freeman LM, Webster CR (2004) C-reactive protein concentrations in canine acute pancreatitis. J Vet Emerg Crit Care 14: 183-186.
Jitpean S, Holst BS, Höglund OV, Pettersson A, Olsson U, Strage E, Hagman R (2014) Serum insulin-like growth factor-I, iron, C-reactive protein, and serum amyloid A for prediction of outcome in dogs with pyometra. Theriogenology 82: 43-48.
Kocaturk M, Martinez S, Eralp O, Tvarijonaviciute A, Ceron J, Yilmaz Z (2010) Prognostic value of serum acute-phase proteins in dogs with parvoviral enteritis. J Small Anim Pract 51: 478-483.
Koch A, Voigt S, Kruschinski C, Sanson E, Dückers H, Horn A, Yagmur E, Zimmermann H, Trautwein C, Tacke F (2011) Circulating soluble urokinase plasminogen activator receptor is stably elevated during the first week of treatment in the intensive care unit and predicts mortality in critically ill patients. Crit Care 15:R63.
Kofoed K, Andersen O, Kronborg G, Tvede M, Petersen J, Eugen-Olsen J, Larsen K (2007) Use of plasma C-reactive protein, procalcitonin, neutrophils, macrophage migration inhibitory factor, soluble urokinase-type plasminogen activator receptor and soluble triggering receptor expressed on myeloid cells-1 in combination to diagnose infections: a prospective study. Crit Care 11:R38
Kofoed K, Eugen-Olsen J, Petersen J, Larsen K, Andersen O (2008) Predicting mortality in patients with systemic inflammatory response syndrome: an evaluation of two prognostic models, two soluble receptors, and a macrophage migration inhibitory factor. Eur J Clin Microbiol Infect Dis 27: 375-383.
Kuleš J, Gotić J, Mrljak V, Rafaj RB (2017) Blood markers of fibrinolysis and endothelial activation in canine babesiosis. BMC Vet Res 13:82.
Martin GS, Mannino DM, Eaton S, Moss M (2003) The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 348: 1546-1554.
Matijatko V, Mrljak V, Kiš I, Kučer N, Foršek J, Živičnjak T, Ceron JJ (2007) Evidence of an acute phase response in dogs naturally infected with Babesia canis. Vet Parasitol 144: 242-250.
Millner MM, Franthal W, Thalhammer GH, Berghold A, Aigner RM, Füger GF, Reibnegger G (1998) Neopterin concentrations in cerebrospinal fluid and serum as an aid in differentiating central nervous system and peripheral infections in children. Clin Chem 44: 161-167.
Murr C, Widner B, Wirleitner B, Fuchs D (2002) Neopterin as a marker for immune system activation. Curr Drug Metab 3: 175-187.
Mylonakis M, Ceron J, Leontides L, Siarkou V, Martinez S, Tvarijonaviciute A, Harrus S (2011) Serum acute phase proteins as clinical phase indicators and outcome predictors in naturally occurring canine monocytic ehrlichiosis. J Vet Intern Med 25: 811-817.
Nakamura M, Takahashi M, Ohno K, Koshino A, Nakashima K, Setoguchi A, Tsujimoto H (2008) C-reactive protein concentration in dogs with various diseases. J Vet Med Sci 70: 127-131.
Ok M, Er C, Yildiz R, Col R, Aydogdu U, Sen I, Guzelbektes H (2015). Evaluation of acute phase proteins, some cytokines and hemostatic parameters in dogs with sepsis. Kafkas Univ Vet Fak Derg 21: 761-766.
Spapen H, Hachimi-Idrissi S, Corne L, Huyghens L (2006) Diagnostic markers of sepsis in the emergency department. Acta Clin Belg 61:138-142.
Stephens RW, Pedersen AN, Nielsen HJ, Hamers MJ, Høyer-Hansen G, Rønne E, Brünner N (1997) ELISA determination of soluble urokinase receptor in blood from healthy donors and cancer patients. Clin Chem 43: 1868-1876.
Torrente C, Manzanilla EG, Bosch L, Fresno L, Rivera del Alamo M, Andaluz A, Ruiz de Gopegui R (2015) Plasma iron, C-reactive protein, albumin, and plasma fibrinogen concentrations in dogs with systemic inflammatory response syndrome. J Vet Emerg Crit Care 25: 611-619.
Tostes Oliveira S, Belle Messick J, Welker Biondo A, Pires Santos A, de Sá Guimarães AM, Sidi Mohamed A, Diaz González FH (2010) Serum and urinary C-reactive protein concentrations in dogs with leptospirosis. Acta Sci Vet 38: 245-249.
Tothova C, Nagy O, Kovac G (2014) Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review. Vet Med (Praha) 59: 163-180.
Viitanen SJ, Laurila HP, Lilja-Maula LI, Melamies MA, Rantala M, Rajamäki MM (2014) Serum C-reactive protein as a diagnostic biomarker in dogs with bacterial respiratory diseases. J Vet Intern Med 28:84-91.
Wittenhagen P, Kronborg G, Weis N, Nielsen H, Obel N, Pedersen SS, Eugen-Olsen J (2004) The plasma level of soluble urokinase receptor is elevated in patients with Streptococcus pneumoniae bacteraemia and predicts mortality. Clin Microbiol Infect 10: 409-415.
Zeng M, Chang M, Zheng H, Li B, Chen Y, He W, Huang C (2016) Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic guidance of sepsis. Am J Emerg Med 34: 375-380.