Effects of strain, perch and nesting area inclusion or exclusion on performance, egg quality traits, and welfare in laying hens housed in enriched cage system
Published:
Apr 19, 2025
Keywords:
egg quality enriched cage system genotype laying hen production performance welfare
Abstract
This study examined the impacts of perch and nesting area inclusion or exclusion on performance, egg quality, and welfare of two laying hen strains. In addition, the study emphasized the effect of hen age on egg quality traits, body region temperatures, and feather score. Lohmann brown (LB) and Lohmann LSL Classic (LW) strains were distributed to enriched cage units; PNO (without perch), PYES (with perch), or NNO (without nesting area), and NYES (with nesting area) at 20 weeks of age. It was identified that LB hens were heavier and had a higher comb, breast region, and footpad surface temperature than LW hens (p<0.01). Hens reared in PNO cages were heavier at 50% egg production than those reared in PYES cages (p<0.05). However, eggs with meat and blood spots in albumen were higher in PYES cages than in PNO cages (p<0.05). Additionally, hens reared in PYES cages had a higher footpad surface temperature than those in PNO cages. Hens reared in NNO cages reached 50% egg production earlier, had a higher comb and rectal temperature, and better feather scores compared to those reared in NYES cages (p<0.05; p<0.01). Eggs with a darker yolk color were obtained from NYES cages than from NNO cages (p<0.05). Furthermore, the effect of nesting area on tonic immobility (TI) approached a significant level (p=0.054), with a shorter duration of TI for hens reared in NYES cages than those in NNO cages. Blood parameters including heterophil-to-lymphocyte ratio were similar between strains, and perch and nesting area treatments (p>0.05). Age effects were observed on average egg weight, and egg quality traits apart from shape index, meat-blood spots in the yolk, feather score and body region temperatures (p<0.01), and egg-laying time impacted average egg weight (p<0.01). The study suggests no substantial evidence that the exclusion of a perch or nesting area in the enriched cages compromises performance and welfare measures in hens. In addition, relatively small differences were observed between the genotypes.
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Amiri, A., Şekeroğlu, A., Tainika, B., Akyol, A., Şentürk, Y., Duman, M., Abacı, S., & Gür, F. (2025). Effects of strain, perch and nesting area inclusion or exclusion on performance, egg quality traits, and welfare in laying hens housed in enriched cage system. Journal of the Hellenic Veterinary Medical Society, 76(1), 8883–8898. https://doi.org/10.12681/jhvms.38250
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- Vol. 76 No. 1 (2025)
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- Research Articles
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References
Akyol A, Şekeroğlu A, Tainika B, Şentürk YE, Duman M, Abacı SH,
Gür FM (2024) Effects of Strain and Stocking Density on Performance,
Egg Size Distribution, Egg Quality, and Welfare in Laying
Hens Housed in Enriched Cage System. Braz J Poult Sci 26 (3):eRBCA-
-1929.
Alig BN, Ferket PR, Malheiros RD, Anderson KE (2023a) The effect of
housing environment on egg production, USDA egg size, and USDA
grade distribution of commercial white egg layers. Poultry 2 (2):204-
Alig BN, Ferket PR, Malheiros RD, Anderson KE (2023b) The effect of
housing environment on commercial brown egg layer production.
USDA grade and USDA size distribution. Animal 13 (4):694.
Alm M, Tauson R, Holm L, Wichman A, Kalliokoski O, Wall H (2016)
Welfare indicators in laying hens in relation to nest exclusion.
Poult Sci 95 (6):1238-1247.
Bari MS, Cohen-Barnhouse AM, Campbell DLM (2020) Early rearing
enrichments influenced nest use and egg quality in free-range laying
hens. Animal 14 (6):1249-1257.
Barnett JL, Tauson R, Downing JA, Janardhana V, Lowenthal J W, Uşak
KL, Cronin GM (2009) The effects of a perch, dust bath, and nest
box, either alone or in combination as used in furnished cages, on the
welfare of laying hens. Poult Sci 88 (3):456-470.
Baylan M, Kursun K, Abdallah N, Celik LB, Yenilmez F, Kutay H (2024)
The effect of housing systems on the growth, egg production, overall
egg weight and egg quality traits of a new Turkish laying hen hybrid,
Akbay. Braz J Poult Sci 26(3): eRBCA-2024.
Bist RB, Subedi S, Chai L, Regmi P, Ritz CW, Kim WK, Yang X (2023)
Effects of perching on poultry welfare and production: a review. Poultry
(2):134-57.
Calik J, Obrzut J (2023) Influence of genotype on productivity and egg
quality of three hen strains included in a biodiversity program. Animal
(11):1848.
Campo JL, Gil MG (1998) Internal inclusions in brown eggs: relationships
with fearfulness and stress. Poult Sci 77 (12):1743-1747.
Carter TC (1975) The hen’s egg: A rapid method for routine estimation of
flock mean thickness. Br Poult Sci 16 (2):131-143.
Casey-Trott TM, Guerin MT, Sandilands V, Torrey S, Widowski TM
(2017) Rearing system affects prevalence of keel-bone damage in
laying hens: a longitudinal study of four consecutive flocks. Poult Sci
(7):2029-239.
Dedousi A, Stojčić MĐ, Sossidou E (2020) Effects of production systems
on keel bone damage and egg quality of laying hens. VRF 11 (4):299.
DePaoli E, Korver D, Bench C (2024) Effect of pullet rearing environment,
strain and perch shape on perching behaviour, perching biomechanics,
and keel bone damage in enriched-housed laying hens. Appl
Anim Behav Sci 272:106187.
Directive EU (1999) Council Directive 99/74/EC of 19 July 1999 Laying
down minimum standards for the protection of laying hens. OJEU
:53-57.
Duncan ET, Appleby MC, Hughes BO (1992) Effect of perches in laying
cages on 20 welfare and production of hens. Br. Poult Sci 33 (1):
-35.
Eleroğlu H (2021) Determining the effects of oviposition time and age
differences on calculated egg parameters of white layer pure line.
TURJAF 34. (sp): 2627-2634.
Eleroğlu H, Taşdemir AN (2020) Effect of laying time and age-related
change on egg characteristics in brown layer pure lines. TURJAF 8
(11): 2498-2506.
Engel JM, Widowski TM, Tilbrook AJ, Butler KL, Hemsworth PH (2019)
The effects of floor space and nest box access on the physiology and
behavior of caged laying hens. Poult Sci 98 (2): 533-547.
Gross WB, Siegel HS (1983) Evaluation of the heterophil/lymphocyte
ratio as a measure 4 of stress in chickens. Avian Dis 27 (4): 972-979.
Hammershøj M, Kristiansen GH, Steenfeldt S (2021) Dual-purpose poultry
in organic egg production and effects on egg quality parameters.
Foods 10 (4): 897.
Hassan S, Habashy W, Ghoname M, Elnaggar A (2023) Blood hematology
and biochemical of four laying hen strains exposed to acute heat
stress. Int J Biometeorol 67 (4): 675-686.
Haugh RR (1937) The Haugh Unit for Measuring Egg Quality. U.S. Egg
and Poultry Magazine 43: 552-573.
IBM Corp (2012) IBM SPSS Statistics for Windows. Version 21.0. New
York, NY, 12 United States of America USA: Armonk, IBM Corp.
Jeffrey FP (1945) Blood and meat spots in chicken eggs. Poult Sci 24 (4):
-374.
Jensen, LS, Sauter E A, Stadelman WJ (1952) The detection and disintegration
of blood spots as related to age of eggs. Poult Sci 31 (3):
-387.
Jones RB, Faure JM (1981) Tonic immobility (“righting time”) in laying
hens housed in 1 cages and pens. Appl Anim Ethol 7 (4): 369-372.
Kamar GAR, Khalifa MAS (1964) The effect of environmental conditions
on body temperature of fowls. Br Poult Sci 5 (3): 235-244.
Käppeli S, Gebhardt-Henrich SG, Fröhlich E, Pfulg A, Schäublin H, Stoffel
MH (2011) Effects of housing, perches, genetics, and 25-hydroxycholecalciferol
on keel bone deformities in laying hens. Poult Sci 90
(8):1637-1644.
Ketta M, Tůmová E, Englmaierová M, Chodová D (2020) Combined effect
of genotype, housing system, and calcium on performance and
eggshell quality of laying hens. Animal 10 (11): 2120.
Kim DH, Lee YK, Kim SH, Lee KW (2020) The impact of temperature
and humidity on the performance and physiology of laying hens. Animal
(1): 56.
Krawczyk J, Lewko L, Sokołowicz Z, Koseniuk A, Kraus A (2023) Effect
of Hen Genotype and Laying Time on Egg Quality and Albumen Lysozyme
Content and Activity. Animal 13 (10): 1611.
Kursun K, Abdallah N, Boga YE, Baylan M (2024) The influence of different
production systems on the welfare of a new commercial layer
hen hybrid. Braz J Poult Sci 26 (01):eRBCA-2023.
Lay Jr DC, Fulton RM, Hester PY, Karcher DM, Kjaer JB, Mench JA,
Mullens BA, Newberry RC, Nicol CJ, O’Sullivan NP, Porter RE
(2011) Hen welfare in different housing systems. Poult Sci 90 (1):
-294.
Leeson S, Summers JD (1987) Effect of immature body weight on laying
performance. Poult Sci 66 (12):1924-1928.
Lohmann (2021). Management guide. Alternative systems. Lohmann
management-systems_EN.pdf. 2021. Accessed online 17 July 2024.
Lordelo M, Cid J, Cordovil CM, Alves SP, Bessa RJ, Carolina I (2020)
A comparison between the quality of eggs from indigenous chicken
breeds and that from commercial layers. Poult Sci 99 (3): 1768-1776.
Madison ME, Perez AM (1994) US Egg and Poultry Statistical Series,
-92.
Morrissey KL, Brocklehurst S, Baker L, Widowski TM, Sandilands V
(2016) Can non beak treated hens be kept in commercial furnished
cages? Exploring the effects of strain and extra environmental enrichment
on behaviour, feather cover, and mortality. Animal 6 (3): 17.
Nalbandov AV, Card LE (1944) The problem of blood clots and meat spots
in chicken eggs. Poult Sci 23 (3): 170-180.
Nasr MA, Murrell J, Nicol CJ (2013) The effect of keel fractures on egg
production, feed and water consumption in individual laying hens. Br
Poult Sci 54 (2):165-170.
Nasr MA, Nicol CJ, Murrell JC (2012) Do laying hens with keel bone
fractures experience pain? Anim Welf 21:127-135.
Nicol CJ (2015) The behavioural biology of chickens. London, United
Kingdom: CABI.
Rakonjac S, Dosković V, Bošković SB, Škrbić Z, Lukić M, Petričević
V, Petrović DM (2021) Production performance and egg quality of
laying hens as influenced by genotype and rearing system. Braz J
Poultry Sci 23 (02): 1-8.
Samiullah S, Omar AS, Roberts J, Chousalkar K (2017) Effect of production
system and flock age on eggshell and egg internal quality
measurements. Poult Sci 96 (1): 246-258.
Samiullah S, Roberts JR, Chousalkar KK (2014) Effect of production system
and flock age on egg quality and total bacterial load in commercial
laying hens. J Appl Poult Res 23 (1): 59-70.
Şekeroğlu A, Duman M, Tahtalı Y, Yıldırım A, Eleroğlu H (2014) Effect
of cage tier and age on performance, egg quality and stress parameters
of laying hens. S Afr J Anim Sci. 44 (3): 288-297.
Sekeroglu A, Sarica M, Demir E, Ulutas Z, Tilki M, Saatçi M, Omed H
(2010) Effects of different housing systems on some performance
traits and egg qualities of laying hens. J Anim Vet Adv 9 (12): 1739-
Şekeroğlu A, Şentürk YE, Tainika B, Duman M, Akyol A (2024) The impact
of laying hen age, egg-laying time, cage tier, and cage direction
on egg quality traits in hens in an enriched cage system. Braz J Poultry
Sci 26 (2): eRBCA-2024.
Sharma MK, McDaniel CD, Kiess AS, Loar II RE, Adhikari P (2022) Effect
of housing environment and hen strain on egg production and egg
quality as well as cloacal and eggshell microbiology in laying hens.
Poult Sci 101 (2): 101595.
Sherwin CM, Richards GJ, Nicol CJ (2010) Comparison of the welfare
of layer hens in 4 production systems in the UK. Br Poult Sci 51
(4):488-499.
Smith AJ, Oliver J (1971) Some physiological effects of high environmental
temperatures on the laying hen. Poult Sci 50 (3): 912-925.
Sözcü A, İpek A, Oguz Z, Gunnarsson S, Riber AB (2021) Comparison of
performance, egg quality, and yolk fatty acid profile in two Turkish
genotypes (Atak-S and Atabey) in a free-range system. Animal 11
(5): 1458.
Stratmann A, Fröhlich EK, Harlander-Matauschek A, Schrader L, Toscano
MJ, Würbel H, Gebhardt-Henrich SG (2015) Soft perches in an
aviary system reduce incidence of keel bone damage in laying hens.
PloS One 10 (3):e0122568
Tainika B, Şekeroğlu A (2020) Effect of production systems for laying
hens on hen welfare. TURJAF 8 (1): 239 245.
Tainika B, Şekeroğlu A, Abacı SH, Akyol A, Duman M, Şentürk YE, Avcı
M (2024b) Evaluation of production performance parameters of two
laying hen strains housed in four housing environments. Braz J Poultry
Sci 26 (2): eRBCA-2023-1885.
Tainika B, Şekeroğlu A, Akyol A, Abaci SH, Gür FM (2024a) The effect
of housing environment (deep litter with or without access to different
plant species outdoor) on welfare and behavior across two strains of
laying hens. Turk J Vet Anim Sci 48 (1): 1-16.
Tainika B, Şekeroğlu A, Akyol A, Şentürk YE, Abaci SH, Duman M
(2024c) Effects of Age, Housing Environment, and Strain on Physical
Egg Quality Parameters of Laying Hens. Braz J Poult Sci 26 (03):eRBCA-
-1911.
Tarım B, Demirtaş ŞE, Bozkurt M, Üstündağ AÖ, Eray O, Ahlat OZ,
Bayır HÖ, Akşit H, Kamanlı S, Özkan S, Yalçın S (2024). Feed form
and perch design do not interact to production performance, gastrointestinal
tract traits, behaviour and welfare of laying hens reared in
enriched cages. Br Poult Sci 65 (2):165-178.
Tauson R, Abrahamsson P (1994) Foot and skeletal disorders in laying
hens: Effects of perch design, hybrid, housing system and stocking
density. Acta Agric Scand A Anim Sci 44 (2):110-9.
Tauson R, Kjaer J, Maria GA, Cepero R, Holm KE (2005) Applied scoring
of integument and health in laying hens. Anim Sci Pap Rep 23 8
(Suppl 1): 153-159.
Tok S, Şekeroğlu A, Duman M, Tainika B (2022) Effect of age, stocking
density, genotype, and cage tier on feather score of layer pure lines.
Turk J Vet Anim Sci 46 (1): 115-123.
Tůmová E, Skřivan M, Englmaierová M, Zita L (2009) The effect of genotype,
housing system and egg collection time on egg quality in egg
type hens. Czech J Anim Sci 54 (1): 17-23.
Van Mourik S, Alders BP, Helderman F, Van De Ven LJ, Koerkamp PG
(2017) Predicting hairline fractures in eggs of mature hens. Poult Sci
(6):1956-62.
Wei H, Feng Y, Ding S, Nian H, Yu H, Zhao Q , Bao H, Zhang R (2022)
Keel bone damage affects behavioral and physiological responses related
to stress and fear in two strains of laying hens. J Anim Sci 100
(4): skac076.
Welfare Quality (2009) Welfare Quality Assessment Protocol for Poultry
(Broilers and Laying Hens). Lelystand, The Netherland: Welfare
Quality Consortium.
Yılmaz Dikmen B, Ipek A, Şahan Ü, Sözcü A, Baycan SC (2017) Impact
of different housing systems and age of layers on egg quality characteristics.
Turk J Vet Anim Sci 41 (1): 77-84.
Yurtseven EP, Şekeroğlu A, Tainika B, Duman M, and Şentürk YE (2021)
Effect of production system and age on egg quality parameters: A case
of Niğde Province Çamardı District, Turkey. TURJAF 9 (8): 1407-
Zurak D, Slovenec P, Janječić Z, Bedeković XD, Pintar J, Kljak K (2022)
Overview on recent findings of nutritional and non-nutritional factors
affecting egg yolk pigmentation. World’s Poult Sci J 78 (2): 531-560.
