test New cardiac biomarkers in dogs with parvoviral enteritis: galectin-3 and cardiotrophin-1|Journal of the Hellenic Veterinary Medical Society

New cardiac biomarkers in dogs with parvoviral enteritis: galectin-3 and cardiotrophin-1

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Published: Nov 10, 2025
DOI: https://doi.org/10.12681/jhvms.40024
Keywords:
Cardiotrophin 1 Dog Galectin-3 Parvoviral Enteritis
GS Boluk
Hatay Mustafa Kemal University, Veterinary Faculty, Department of Internal Medicine, Hatay, Turkiye
AS Ozturk
Hatay Mustafa Kemal University, Institute of Health Sciences, Department of Molecular Biochemistry and Genetic (Medicine), Hatay, Turkiye - Gaziantep Islam Science and Technology University, Medicine Faculty, Department of Physiology, Gaziantep, Turkiye
A Ozturk
Gaziantep Islam Science and Technology University, Medicine Faculty, Department of Physiology, Gaziantep, Turkiye
Abstract

Özet: Köpek parvoviral enteriti, aşılamanın bulunmasına rağmen, yavru köpeklerde yüksek mortalite ve morbidite ile ilişkili bir hastalık olmaya devam etmektedir. Sonuç olarak, parvoviral enterit için biyobelirteçlerle ilgili devam eden araştırmalar yürütülmektedir. Bu çalışmanın amacı, parvoviral enterit (PVE) tanısı konulan köpeklerde rutin parametrelerle birlikte ilk kez kardiyotrofin-1 (CT-1) ve galectin-3'ü (GAL-3) değerlendirmektir. PVE grubu üç kategoriye ayrıldı: 3 aylıktan küçük ve aşılanmamış köpekler (n = 8), 3 aylıktan büyük ve aşılanmamış köpekler (n = 15) ve birinci veya ikinci aşılamayı almış köpekler (n = 10). Sekiz sağlıklı köpekten oluşan bir kontrol grubu da dahil edildi. GAL-3, CT-1, CK-MB ve CK serum düzeyleri, kontrol grubuyla karşılaştırıldığında parvoviral enteritli (PVE) tüm köpeklerde anlamlı derecede yüksekti. Toplam lökosit sayısı tüm PVE gruplarında önemli ölçüde azaldı. 3 aylıktan küçük köpeklerde, Paketlenmiş Hücre Hacmi (PCV) değerleri kontrol grubu değerlerine benzerdi. Kreatinin seviyeleri tüm PVE gruplarında önemli ölçüde azaldı. Sonuç olarak, rutin biyokimyasal parametrelerin aksine, yüksek GAL-3, CT-1 seviyeleri, CK-MB ve CK ile birlikte, tatmin edici duyarlılık ve özgüllük göstererek PVE için biyobelirteç olarak potansiyellerini düşündürmektedir. Bu biyobelirteçler ayrıca yeni terapötik hedefleri temsil edebilir.

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References
-Acciacca RA, Sullivan LA, Webb TL, Johnson V, Dow SW. Clinical evaluation
of hyperimmune plasma for treatment of dogs with naturally
occurring parvoviral enteritis. The Journal of Veterinary Emergency and
Critical Care 2020; 30 (5): 525-533. https://DOI : 10.1 111/vec.12987
- Lamm CG, Rezabek GB. Parvovirus infection in domestic companion
animals. Veterinary Clinics of North America: Small Animal Practice
; 38(4) :837-ix. https://DOI : 10.1016/j.cvsm.2008.03.008
- Decaro N, Desario C, Addie DD, Martella V, Vieira MJ, et al. The study
molecular epidemiology of canine parvovirus, Europe. Emerging
Infectious Diseases 2007; 13(8): 1222-1224. https://DOI : 10.3201/
eid1308.070505
- Goddard A, Leisewitz AL. Canine parvovirus. Veterinary Clinics of North
America: Small Animal Practice 2010; 40: 1041–1053. https://DOI :
1016/j.cvsm.2010.07.007
- McClure V, van Schoor M, Thompson PN, Kjelgaard-Hansen M, Goddard
A. Evaluation of the use of serum C-reactive protein concentration to predict outcome in puppies infected with canine parvovirus. Journal of
the American Veterinary Medical Association 2013; 243(3): 361-366.
https://DOI : 10.2460/javma.243.3.361
- Mazzaferro EM. Update on Canine Parvoviral Enteritis. Veterinary Clinics
of North America: Small Animal Practice 2020; 50(6): 1307-1325.
https://doi: 10.1016/j.cvsm.2020.07.008
- Er C, Ok M. Levels of Cardiac Biomarkers and Coagulation Profiles in
Dogs with Parvoviral Enteritis. Kafkas Universitesi Veteriner Fakultesi
Dergisi 2015; 21: 383-388. https://DOI : 10.9775/kvfd.2014.12575
- Stojanovic BS, Stojanovic B, Milovanovic J, Arsenijević A, Dimitrijevic
Stojanovic M, et al. The Pivotal Role of Galectin-3 in Viral Infection: A
Multifaceted Player in Host-Pathogen Interactions. Int J Mol Sci 2023;
(11):9617. https://doi: 10.3390/ijms24119617.
- Zivlas C, Triposkiadis F, Psarras S, Giamouzis G, Skoularigis I, et al. Left
atrial volume index in patients with heart failure and severely impaired
left ventricular systolic function: the role of established echocardiographic
parameters, circulating cystatin C and galectin-3. Therapeutic
Advances in Cardiovascular Disease 2017; 11(11): 283-295. https://doi:
1177/1753944717727498
- Wang XY, Zhang F, Zhang C, Zheng LR, Yang J. The Biomarkers for
Acute Myocardial Infarction and Heart Failure. BioMed Research International
; 2018035. https://doi:10.1155/2020/2018035
- Archer J. Cardiac biomarkers: a review. Comparative Clinical Pathology
; 12(3): 121-128. https://doi.org/10.1007/s00580-003-0488-9
- Gkaliagkousi E, Gavriilaki E, Nikolaidou B, Chatzopoulou F, Anyfanti P,
et al. Association between cardiotrophin 1 levels and central blood pressure
in untreated patients with essential hypertension. American Journal
of Hypertension 2014; 27(5): 651-655. https://doi:10.1093/ajh/h pt238
- Benigni F, Sacco S, Pennica D, Ghezzi P. Cardiotrophin-1 inhibits tumor
necrosis factor production in the heart and serum of lipopolysaccharide-
treated mice and in vitro in mouse blood cells. The American
Journal of Pathology 1996; 149(6): 1847-1850.
- Sheng Z, Knowlton K, Chen J, Hoshijima M, Brown JH, Chien KR.
Cardiotrophin 1 (CT-1) inhibition of cardiac myocyte apoptosis via a
mitogen-activated protein kinase-dependent pathway. Divergence from
downstream CT-1 signals for myocardial cell hypertrophy. The Journal
of Biological Chemistry 1997; 272(9): 5783-5791. https://doi:10.1074/
jbc.272.9.5783
- Sime TA, Powell LL, Schildt JC, Olson EJ. Parvoviral myocarditis in
a 5-week-old Dachshund. The Journal of Veterinary Emergency and
Critical Care (San Antonio) 2015; 25(6): 765-769. https://doi:10.1111/
vec.12347
- Robinson WF, Wilcox GE, Flower RL. Canine parvoviral disease: experimental
reproduction of the enteric form with a parvovirus isolated
from a case of myocarditis. Veterinary Pathology 1980; 17(5): 589–599.
- Fagbohun OA, Jarikre TA, Alaka OO, Adesina RD, Ola OO, et al. Pathology
and molecular diagnosis of canine parvoviral enteritis in Nigeria:
case report. Comparative Clinical Pathology 2020; 29(4): 887-893.
https://doi: 10.1007/s00580-020-03127-7.
- Kalli I, Leontides LS, Mylonakis ME, Adamama-Moraitou K, Rallis T,
Koutinas AF Factors affecting the occurrence, duration of hospitalization
and final outcome in canine parvovirus infection. Research in Veterinary
Science 2010; 89(2): 174-178. https://doi:10.1016/j.rvsc.2010.02.013
- Pollock RV, Coyne MJ. Canine parvovirus. Veterinary Clinics of North
America: Small Animal Practice 1993; 23(3): 555-568. https://doi:
1016/s0195-5616(93)50305-4
- Carr-Smith S, Macintire DK, Swango LJ. Canine parvovirus: Part 1.
Pathogenesis and vaccination. Compendium: Continuing Education for
Veterinarians 1997; 19: 125-133.
- Bessman SP, Carpenter CL. The creatine-creatine phosphate energy
shuttle. Annu Rev Biochem 1985; 54:831–62
- Franco LG, Fioravanti MC, Damasceno AD, Borges AC, Soares LK, et
al. Assessment of serum enzymatic markers of cardio-myocytes injury
in female dogs submitted to ketamine S(+), atropin and xylazine association.
Acta Cirúrgica Brasileira 2009; 24(1): 36-42. https://doi:10.1590/
s0102-86502009000100008
- Kubesy AA, Saleh IA, Torad FA, Salem IS, Ali ME. The prognostic
value of electrocardiography for canine parvovirus infection in German
shepherd puppies. Bıoscıence Research 2020; 16(1): 234-241.
- Bhat AA, Wadhwa DR, Imran S, Chander V. Plasma concentration of
creatine kinase-MB isoenzyme in dogs with intestinal form of canine
parvovirus-2 infection. Comp Clin Pathol 2014; 23: 665–667. https://
doi.org/10.1007/s00580-012-1667-3
- Eregowda CG, De UK, Singh M, Prasad H, Akhilesh, et al. Assessment
of certain biomarkers for predicting survival in response to treatment in
dogs naturally infected with canine parvovirus. Microbial Pathogenesis
; 149: 104485. https://doi:10.1016/j.micpath.2020.104485
Ghosh A, Datta P, Dhingra M. Higher Levels of Creatine Kinase MB
(CK-MB) Than Total Creatine Kinase (CK): A Biochemistry Reporting
Error or an Indicator of Other Pathologies? Cureus. 2023; 15: e50792.
doi: 10.7759/cureus.50792.
- Rose-John S. Interleukin-6 Family Cytokines. Cold Spring Harbor
Perspectives in Biology 2018; 10(2): a028415. https://doi: 10.1101/
cshperspect.a028415
- Carneros D, Santamaría EM, Larequi E, Vélez-Ortiz JM, Reboredo M,
et al. Cardiotrophin-1 is an anti-inflammatory cytokine and promotes
IL-4-induced M2 macrophage polarization. FASEB J. 2019; 33(6): 7578-
doi: 10.1096/fj.201801563R.
- Perretta-Tejedor N, Price KL, Jafree DJ, Pomeranz G, Kolatsi-Joannou
M, et al. Cardiotrophin-1 therapy reduces disease se-verity in a murine
model of glomerular disease. Physiol Rep. 2024; 12(13):e16129. https://
doi: 10.14814/phy2.16129.
- Liu YH, D’Ambrosio M, Liao TD, Peng H, Rhaleb NE, et al. N-acetyl-seryl-
aspartyl-lysyl-proline prevents cardiac remodeling and dysfunction
induced by galectin-3, a mammalian adhesion/growth-regulatory lectin.
The American Journal of Physiolo-gy-Heart and Circulatory Physiology
; 296(2): H404-H412. https://DOI: 10.1152/ajpheart.00747.2008
- Lindner D, Westermann D. Firefighting in viral myocarditis - Extinguish
galectin-3 to control the inflamed heart?. The Journal of Molecular
and Cellular Cardiology 2015; 85: 226-228. https://doi: 10.1016/j.
yjmcc.2015.06.004.
- Passino C, Barison A, Vergaro G, Gabutti A, Borrelli C, et al. Markers of
fibrosis, inflammation, and remodeling pathways in heart failure. Clinica
Chimica Acta 2015; 443: 29-38. https://doi: 10.1016/j.cca.2014.09.006
Martínez-Martínez E, Brugnolaro C, Ibarrola J, Ravassa S, Buonafine
M, López B, Fernández-Celis A, Querejeta R, Santamaria E, Fernández-
Irigoyen J, Rábago G, Moreno MU, Jaisser F, Díez J, González A,
López-Andrés N. CT-1 (Cardiotrophin-1)-Gal-3 (Galectin-3) Axis in
Cardiac Fibrosis and Inflammation. Hypertension 2019; 73: 602-611.
doi: 10.1161/HYPERTENSIONAHA.118.11874.
Carneros D, Santamaría EM, Larequi E, Vélez-Ortiz JM, Reboredo M,
Mancheño U, Perugorria MJ, Navas P, Romero-Gómez M, Prieto J,
Hervás-Stubbs S, Bustos M. Cardiotrophin-1 is an anti-inflammatory
cytokine and promotes IL-4-induced M2 macrophage polarization.
FASEB J 2019; 33: 7578-7587. doi: 10.1096/fj.201801563R.
- Sykes JE. Canine parvovirus infections and other viral enteritides. Canine
and Feline Infectious Diseases 2014; 141-151. https://doi:10.1016/
B978-1-4377-0795-3.00014-4
- Macintire DK, Smith-Carr S. Canine parvovirus. Part II. Clinical signs,
diagnosis, and treatment. Compendium on Continuing Education for
the Practising Veterinarian 1997; 19(3): 291–300.
- Goddard A, Leisewitz AL, Christopher MM, Duncan NM, Becker PJ.
Prognostic usefulness of blood leukocyte changes in canine parvoviral
enteritis. Journal of Veterinary Internal Medicine 2008; 22(2): 309-316.
https://doi:10.1111/j.1939-1676.2008.0073.x
- Macartney L, McCandlish IA, Thompson H, Cornwell HJ. Canine parvovirus
enteritis 1: Clinical, haematological and pathological features
of experimental infection. Veterinary Record 1984; 115(9):201-210.
- Mylonakis ME, Kalli I, Rallis TS. Canine parvoviral enteritis: an update
on the clinical diagnosis, treatment, and prevention. Veterinary medicine
(Auckland, N.Z.) 2016; 7: 91-100. https://doi:10.2147/VMRR.S80971
- Schetters TP, Kleuskens JA, Van De Crommert J, De Leeuw PW, Finizio
AL, Gorenflot A. Systemic inflammatory responses in dogs experimentally
infected with Babesia canis; a haematological study. Veterinary Parasitology
; 162(1-2): 7-15. https://doi:10.1016/j.vetpar.2009.02.012
- Braun J, Lefebvre H, Watson A. Creatinine in the Dog: A Review.
Veterinary Clinical Pathology 2003; 32(4): 162-179. https://doi:
1111/j.1939-165x.2003.tb00332.x.
- van den Berg MF, Schoeman JP, Defauw P, Whitehead Z, Breemersch A,
et al. Assessment of acute kidney injury in canine parvovirus infection:
Comparison of kidney injury biomarkers with routine renal functional
parameters. Veterinary Journal 2018; 242: 8-14. https://doi:10.1016/j.
tvjl.2018.10.002
- Prowle JR, Kolic I, Purdell-Lewis J, Taylor R, Pearse RM, Kirwan CJ.
Serum creatinine changes associated with critical illness and detection
of persistent renal dysfunction after AKI. Clinical Journal of the American
Society of Nephrology 2014; 9(6): 1015-1023. https://doi:10.2215/
CJN.11141113
- Polzin DJ, Osborne CA, Lilich JP Effects of dietary protein/phosphate
restriction in normal dogs and dogs with chronic renal failure.
Journal of Small Animal Practise 1991; 32: 289-295. https://doi.
org/10.1111/j.1748-5827.1991.tb00933.x