Protection of piglets immunized with a cloned pseudorabies virus

PDF (English)
Publicado: Νοε 30, 2017
DOI: https://doi.org/10.12681/jhvms.15090
S. A. ABDELWAHID
Department of Preventive Medicine, Faculty of Veterinary Medicine, University of Khartoum
IMA ELJALII
Department of Preventive Medicine, Faculty of Veterinary Medicine, University of Khartoum
Resumen

The potential of pseudorabies virus (PrV) clone (mAlp) as an immunizing agem against Aujeszky's disease (AD) in swine was invesτigaτed in this study. The pathogenicity, immunogenicity and protective efficiency induced by the virus were the major focus of the study. An indirect enzyme-linked immunosorbent assay was used to measure antibody responses to the virus. The virus was proved non-pathogenic for piglets up to the dose 10 plaque forming unit (p.f.u.). The antibody titres to the virus were correlated with the immunization dose. The difference in the antibody levels for the doses 10 and 10 p.f.u. was nonsignificant (p<0.01), but between the dose 10 and 10 , was significant (p<0.05). Following challenge of immunized animals with the virulent PrV-CD strain, total protection of piglets was observed even at the immunizing dose 10 p.f.u. In conclusion, PrVmAlp was non-pathogenic, highly immunogenic and protective against virulent PrV challenge in piglets, hence suggested as an efficacious and safe vaccine candidate against the disease.

Article Details
  • Sección
  • Research Articles

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Descargas
Los datos de descargas todavía no están disponibles.
Citas
Ali, A.S. (1999). Immune response to a live virus vaccine candidate and protection against Pseudorabies virus infection. PhD thesis, Putra University, Malaysia.
Ali, AS. and Mohd-Azmi, M.L.(1997). Measurement of antibody response to Aujeszky's disease virus in mouse sera by indirect ELISA. The 9th Vet. Assoc. Malaysia Scientific Congress, Penang. 219-222.
Ali, A.S., Mohd-Azmi, M.L. and Aini, I. (1998). Immune responses to a non-pathogenic Pseudorabies virus (mAlp) in mice. J. Vet. Malaysia 10(2): 55-62.
Ali, AS., Mohd-Azmi, M.L., Negrin, A. and Castagnao, M. (2004). Effect of route of inoculation and antigen preparation on the immunogenicity of non-pathogenic Pseudorabies virus clone. Large Animals Review, Ann 10, n.6 December 27-30.
Ben-Porat, T., De Marchioli, J.M. and Kaplan, A.S. (1974). Characterization of defective interfering viral particles present in a population of Pseudorabies virions. Virol. 61: 29-37.
Clark, M.F. and Barbara, D.J. (1987). A method for the quantitative analysis of ELISA data. J. Virol. Meth. 15: 213-222.
Cowen, P., Li, S., Guy, J.S. and Erickson, G.A (1990). Reactivation of latent Pseudorabies virus infection in vaccinated commercial sows. Am. J. Vet. Res. 51(3): 354-358.
Ferrari M, Brack A, Romanelli MG, Mettenleiter TC, Corradi A, Dal Mas Ν, Losio MN, Suini R, Pinoni C, Pratelli A.( 2000). A study of the ability of a TK-negative and gl/gE-negative Pseudorabies virus (PRV) mutant inoculated by different routes to protect pigs against PRV infection. J Vet Med Β Infect Dis Vet Public Health. 47(10):753-62.
Ficinska J, Bienkowska-Szewczyk K, Jacobs L, Plucienniczak G, Plucienniczak A, Szewczyk Β (2003). Characterization of changes in the short unique segment of Pseudorabies virus BUK-TK900 (Suivac A) vaccine strain. Arch Virol. 148(8):1593-612.
Kluge, J.P., Beran, G.W., Hill, H.T. and Platt, K.B. (1992). Pseudorabies (Aujeszky's disease). In: Leman, A.D., Straw, B., Mengeling, W.L., DAllaire, S., Taylor, D.J. Diseases of swine, 7th edn. Iowa state University Press, Ames, USA. pp 312-323.
Kritas, S.K., Nauwynck, H. and Pensaert, M.B. (1995). Dissemination of wild-type and gC, gE and gl-deleted mutants of Aujeszky's disease virus in the maxillary nerve and trigeminal ganglion of pigs after intranasal inoculation. J. Gen. Virol. 76: 2063-2066.
Kritas, S.K., Pensaert, M.B. and Mettenleiter, T.C. (1994). Invasion and spread of single glycoprotein deleted mutants of Aujeszky's disease virus (ADV) in the trigeminal nervous pathway of pigs after intranasal inoculation. Vet. Microbiol. 40: 323-334.
Martin, S. and Wardley, R.C. (1987). Local humoral and cellular responses in Aujeszky's disease virus infection in pigs. Res. Vet. Sci. 42: 170-174.
Pensaert, M.B., De Smet, K. and De Waele, K. (1990). Extent and duration of virulent virus excretion upon challenge of pigs vaccinated with different glycoprotein-deleted Aujeszky's disease vaccines. Vet. Microbiol. 22: 1-11.
Pensaert, M.B., Gielkens, A.L.J., Lomniczi, B., Kimman, T.G. Vannier, P. and Eloit, M. (1992). Round table on control of Aujeszky's disease and vaccine development based on molecular biology. Vet. Microbiol. 33: 53-67.
Schoenbaum, M.A., Beran, W.G. and Murphy, D.P. (1990). Pseudorabies virus latency and reactivation in vaccinated swine. Am. J. Vet. Res. 51(3): 334-337.
Stegeman, J.A, Tielen, M.J.M., Kimman, T.G., Van Oirschot, J.T., Hunneman, W.A, and Berndsen, F. (1994). Intensive regional vaccination with a gl-deleted vaccine markedly reduces Pseudorabies virus infection. Vaccine 12: 527-531.
Vannier, P., Hutet, E., Bourgueil, E. and Cariolet, R. (1991). Level of virulent virus excreted by infected pigs previously vaccinated with different glycoprotein deleted Aujeszky's disease vaccines. Vet. Microbiol. 29: 213-223.
Wittmann, G. Jakubik, J. and Ahi, R. (1980). Multiplication and distribution of Aujeszky's disease (Pseudorabies) virus in vaccinated and non-vaccinated pigs after intranasal infection. Arch Virol. 66: 227-240.