Evidence for the presence of morphine like substance and μ opiate receptor expression in Dicrocoelium dendriticum (Trematoda: Dicrocoeliidae)

PDF (English)
Publicado: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15495
T. S. MOSTAFAEI
Department of Pathobiology, Faculty of Veterinary Medicine
S. NAEM
Department of Pathobiology, Faculty of Veterinary Medicine
M. IMANI
Department of Basic Sciences, Faculty of Veterinary Medicine
N. DALIREZH
Department of Microbiology, Faculty of Veterinary Medicine
Resumen

Parasites for living in host’s body, evade from host’s immune system using variant methods such as production of some molecules with immunosuppressive properties. Of these immunosuppression molecules are morphine and morphine like substances. It can effect on innate and adaptive immunity and also humoral and cellular immunity. The past researches were approved the existence of morphine in the brain and adrenal of beef and in brain of mammalian and also it has been demonstrated as an immune modulator molecule in some invertebrates, some of the nematodes and also a trematode Schistosoma mansoni, But there is no document for morphine evidence in trematode Dicrocoelium dendriticum. Infection with this trematode has much importance either in health of ruminant and economic loos in the meat industry. In addition, this trematode is a zoonosis that there is no proper treatment for it in contrast with the other liver fluke, Fasciola hepatica. We assume that living for a long time in the bile ducts of host is related to morphine like substance as an immune regulatory molecule. We use RP-HPLC method for detection morphine in D. dendriticum and also using reverse transcription polymerase chain reaction (RT-PCR) for μ opioid receptor finding. According to the results D. dendriticum has endogenous morphine like material and also the existence of opioid μ receptor in this trematode was approved. Living in host bile duct for a long time without sever immunopathologycal impact, suggested that probably this worm use of morphine like material as an immunoregulatory molecule and also for escaping from host immune system.

Article Details
  • Sección
  • Research Articles
Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Descargas
Los datos de descargas todavía no están disponibles.
Citas
Cadet P, Stefano G B(1999) Mytilus edulis pedal ganglia express μ opiate receptor transcripts exhibiting high sequence identity with human neuronal mu1. Brain Res Mol Brain Res 74: 242-246.
Carr DJ, Serou M (1995) Exogenous and endogenous opioids as biological response modifiers. Immunopharmacol 31(1): 59-71.
Christrup L L (1997) Morphine metabolites. Acta Anaesthesiol Scand 41(1 Pt 2): 116-122.
Chuang, TK, Killam KF, ChuangLF, Kung H F, Sheng WS, Chao CC, Yu L, Chuang RY (1995). μopioid receptor gene expression in human cell. Biochem Biophys Res Commun 216(3): 922-930.
Freier DO , Fuchs BA (1994). A mechanism of action for morphineinduced immunosuppression: corticosterone mediates morphine induced suppression of natural killer cell activity. J Pharmacol Exp Ther 270(3): 1127-1133.
Gach K, Wyrębska A, Fichna J, Janecka A (2011) The role of morphine in regulation of cancer cell growth. Naunyn-Schmiedeberg’s Archives of Pharmacol 384(3): 221-230.
Goldstein A, Barrett RW, James IF, LowneyLI, Weitz CJ, Knipmeyer LL, Rapoport H (1985) Morphine and other opiates from beef brain and adrenal. Proc Natl Acad Sci U S A 82(15): 5203-5207.
Goumon Y, Casares F, Pryor S, Ferguson L (2000) Ascaris suum, an Intestinal Parasite, Produces Morphine. JI: 339-343.
Jacobs D, Fox M, Gibbon L , Hermosilla C (2016) Veterinary Parasitology: basic concepts.In Principles of Veterinary Parasitology, John Wiley & Sons, Ltd:.pp:45-62
Laurent V, Salzet B, Bernet F, Salzet M (2000) Morphine-like substance in leech ganglia. Eur J Biochem 267(8): 2354-2361.
Leung, M K, Dissous C, Capron A, Woldegaber H, Duvaux-Miret O,Pryor S , Stefano GB (1995) Schistosoma mansoni: the presence and potential use of opiate-like substances. Exp Parasitol 81(2): 208-215.
Mosmann TR, Sad S (1996) The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 17(3): 138-146.
Ninković J, Roy S (2013) Role of the μ opioid receptor in opioid modulation of immune function. Amino acids 45(1): 9-24.
Oka K, Kantrowitz J.D, Spectore S (1985) Isolation of morphine from toad skin. Proc Natl Acad Sci USA 82: 1852-1854.
Owen J A, Punt J, Stanford SA (2009) Innate Immunity. InKUBY Immunology.7thed, W. H. Freeman and Company New York: pp141-182.
Pepe P, Castellano M, Alfano S, Della Pepa ME, TironoV, Piemonte M, Desiderio (2015) Dicrocoelium dendriticum induces autophagic vacuoles accumulation in human hepatocarcinoma cells. Vet Parasitol 7727
Pearce EJ, Sher A (1987) Mechanisms of immune evasion in schistosomiasis. Contrib Microbiol Immunol 8: 219-232.
Pryor SC, Elizee R (2000) Evidence of opiates and opioid neuropeptides and their immune effects in parasitic invertebrates representing three different phyla: Schistosoma mansoni, Theromyzon tessulatum, Trichinella spiralis. Acta Biol Hung 51(2-4):331-341.
Pryor SC, Henry S, Sarfo J (2005) Endogenous morphine and parasitic helminthes. Med Sci Monit 11(6): Ra183-189.
Sonetti D, Mola L, Casares F, Bianchi E, Guarna M, Stefano GB (1999) Endogenous morphine levels increase in molluscan neural and immune tissues after physical trauma. Brain Res 835(2):137-147.
Stefano GB, Digenis A, Spector S, Leung MK, Bilfinger TV, Makman MH, Scharrer B, Abumrad NN (1993) Opiate-like substances in an invertebrate, an opiate receptor on invertebrate and human immunocytes, and a role in immunosuppression. Proc Natl Acad Sci U S A90(23): 11099-11103.
Szkutnik-Fiedler D, Grzeskowiak E, Gaca M, Borowicz M (2011) HPLC-UV determination of morphine in human plasma and its application to the clinical study. Acta Pol Pharm 68(4): 473-479.
Wan Q, Wang X, Wanga Y J, Songa L, Wangb S H, Ho WZ (2008) Morphine suppresses intracellular interferon-α expression in neuronal cells.J Neuroimmunol 199: 1-9.
Weitz, CJ, LowneyLI, Faull KF, Feistner G, Goldstein A (1986) Morphine and codeine from mammalian brain. Proc Natl Acad Sci U S A 83(24): 9784-9788.
Zhu W, Baggerman G, Secor WE, Casares F, Pryor SC, Fricchione GL, Ruiz-Tiben E, Eberhard ML, Bimi L, Stefano GB (2002) Dracunculus medinensis and Schistosoma mansoni contain opiate alkaloids. Ann Trop Med Parasitol 96(3): 309-316.