Effect of Sinefungin on the attachment of the parasite Leishmania to the macrophages cell line J774G8


A. TZORA (Α. ΤΖΩΡΑ)
F. LAWRENCE
M. ROBERT-GERO
Resumen

The effect of Sinefungin on the attachment of the Leishmania strains L. infantum, L. tropica and L. major to the macrophages cell line J774G8 was studied. Inhibition of

the attachment to the surface of macrophages was observed to the Sinefungin sensitive strains L. tropica and L. major. This inhibition was obtained only for Sinefungin pretreated promastigotes (72 hours with 2.6 and 26 Μμ Sinefungin) when incubated with macrophages for 2 and 24 hours. After 2 h incubation period of the promastigote forms of the sensitive L. tropica with macrophages, the inhibition of the attachment was 100%, whereas for the sensitive L. major strain the inhibition was 17 and 36% at a concentration of Sinefungin 2.6 and 26 μΜ respectively. The percentage of the inhibition 24 h post infection was in the range of 52 to 77% for the L. tropica strain and from 73 to 86% for the L. major strain for promastigote pretreated with 2.6 and 26 μΜ Sinefungin respectively. Controls showed that Sinefungin was not toxic for the macrophages when used for 3 days at the above mentioned concentrations. Inhibition of the protozoa - macrophage cell line J774G8 adherence was a common finding leading us to the conclusion that Sinefungin is involved actively into that process.

Article Details
  • Sección
  • Research Articles
Descargas
Los datos de descargas todavía no están disponibles.
Citas
Zuckerman A, Lainson R. Leishmania in: Kreir, JP (Ed) Parasite Protozoa chapt 1977, 3:57-133 Academic Press, New York, San Francisco, London.
Mosser DM, Rosenthal LA. Leishmania-macrophage interactions: multiple receptors, multiple ligands and diverse cellular responses. Seminars in cell biology 1993,4:315-22.
Prina E, Lang T, Glaichenhaus N, Antoine JC. Presentation of the protective parasite antigen LACK by Leishmaniainfected macrophages. J. Immun 1960,154: 4318-27
Bachrach U, Schnur LF, El-on J, Greenblatt CL, Pearlman E, Robert Gero M, Lederer E. Inhibitory activity of Sinefungin and SIBA (5'-Deoxy-5'-S-isobutyl-thioadenosine)
on the growth of promastigotes and amastigotes of different species of Leishmania. FEBS Lett 1980, 121:287-291
Χατζηαντωνίου Μ. Μαζική καλλιέργεια της προμαστιγωτής μορφής του παρασίτου λεϊσμάνια - παρασκευή νέου μονοφασικού υλικού. Διδακτορική διατριβή. Ιατρική Σχολή Πανεπιστημίου Αθηνών 1984.
Κοντός Β. Συμβολή στη μελέτη της λεϊσμανίασης του σκύλου. Κλινική, ορολογική και πειραματική διερεύνηση. Διδακτορική διατριβή. Πανεπιστήμιο Θεσσαλονίκης 1986
Frank C. Serologische Klassifizierung und biochemische indentifizierung von in Griechenland isolierten Leishmania -Stammen (Kinetoplastida: Trypanosomatidae). Dissertation-Universität Tubingen,1991
Tzamouranis N, Schnum LF, Garifallou A, Pateraki E, Serie C. Leishmaniasis in Greece. I. Isolation and Identification of the parasite causing human and canine visceral leishmaniasis. Ann. Trop. Med. and Parasite 1984, 78: 4:363-368.
Tzora-Skoufou A, Lawrence F, Robert-Gero M, and Hadziandoniou M. Recherche de résistants spontanés à la Sinefungine dans les souches de leishmanies provenant de Grece. Bulletin de la Sosiété Française de Parasitologie 1990,8:1:19-24
Chang KP. Human Cutaneus leishmaniasis in a mouse macrophage line:propagation and isolation of intracellular parasites. Sciences 1980, 209:1240-1242
Chang KP, Fong D, Bray RS. Biology of leishmania and leishmaniasis. Elsevier Science Publishers B.V. Part 1, P, 1985:1-30
Skamene E, Gros P. Role of macrophages in resistance against infectious diseases. Clin Immun. All 1983, 3:539560
Pulvertratt RJV, Hogle GF. Stages in the life cycle of Leishmania donovani. Trans R. Soc. Trop. Med. Hyg 1960, 54:191-196
Wyler DJ. In vitro parasite-monocyte interactions in human leishmaniasis. Evidence for an active role of the parasite in attachment. J. Clin. Invest 1982, 70: 82-88
Chang KP. Leishmania donovani: Promastigote-macrophage surface interactions in vitro. Exp. Parasitol 1979, 48:175-189
Sacks DL, Dasilva RP. The generation of infective stage leishmania major promastigotes is associated with the cell surface expression and release of a developmentally regulated glycolipid. J. Immunol,1987:139-3099
Ash C. Macrophages at the centre of infection. Parasitology Today 1991, 7:2-4
Pan AA, McMahon, Pratt D. Monochonal antibodies specific for the amastigote stage of leishmania pifanoi. I. characterization of antigens associated with stage and species - specific determinants. J. Immun 1988,140:2406- 2414
Paolantonacci P, Lawrence F, Nolan LL, Robert-Gero M. Inhibition of leishmanial DNA synthesis by sinefungin. Biochem Pharmacol 1987,36:2813-2820
Sacks DL, Perkins PV. Identification of an infective stage of leishmania promastigotes. Science 1984, 223:1417 – 1419
Dutta M, Bandyopadhyay R, Basu MK. Neoglycosylated liposomes as efficient ligands for the evaluation of specific sugar receptors on macrophages in health and in experimental leishmaniasis. Parasitology 1994,109: 2:139-47
Τζώρα-Σκοΰφου A, Lawrence F, Robert-Gero Μ. Η δράση της σινεφουνγίνης στη βιοσύνθεση των μεγαλομορίων του πρωτόζωου Λεϊσμάνια. 7° Πανελλήνιο Κτηνιατρικό Συνέδριο, Θεσσαλονίκη, 1997
Soteriadou KP, Tzinia AK, Mamalaki A, Phelouzat MA, Lawrence F, Robert-Gero M. Expression of the major surface glycoprotein of leishmania, gp63, in wild-type and sinefungin resistant promastigotes. Eur J Biochem 1994,223:61-68
Artículos más leídos del mismo autor/a