Molecular Diversity Analysis of Jattal and Dera Din Panah Goat Breeds of Pakistan using Microsatellite Markers


T. HUSSAIN
M. SHAHEEN
M. E. BABAR
M. M. MUSTHAFA
A. NADEEM
A. NAWAZ
M. M. JAVED
F. MARIKAR
Resumen
This paper evaluates the genetic diversity of goat breeds in Pakistan, a country blessed with a wide range of goats spread throughout with distinct features contributing to a rich Animal Genetic Resource. The genetic diversity of two goat breeds (Jattal and Dera Din Panah) was assessed with 25 animals representing the two breeds using 16 microsatellite markers. The mean observed and expected heterozygosity of both goat breed populations were observed as 0.83+0.21. The average number of observed alleles was 3.6+1.6 for all loci. The mean polymorphic information content for a goat breed was 0.45, indicating the usefulness of markers panel. Highest Nei’s standard genetic distance (Ds) value of 0.0612 was observed between Jattal and Dera Din Panah goats, and the mean Fst value was 0.013. The measures of genetic variation revealed a good scope for effective improvement, conservation, and designing national breeding policies, in future, for Pakistan goat breeds.
Article Details
  • Sección
  • Research Articles
Descargas
Los datos de descargas todavía no están disponibles.
Citas
Bozzi, R., P. Degl’Innocenti, P. Rivera Diaz, L. Nardi, A. Crovetti, C. Sargenti & A. Giorgetti, 2009. Genetic characterization and breed assignment in five Italian sheep breeds using microsatellite markers. Small Ruminant Research, 85, 50–57.
Bruford, M. W. & R. K. Wayne, 1993. Microsatellites and their application to population genetic studies. Current Opinion in Genetics & Development, 3, 939–943.
FAO, 1985.FAO Animal Production and Health Paper 56 Sheep and Goats in Pakistan. Karachi, Karachi press, pp 25-49.
Fatima, S., C.D. Bhonga, D.N. Ranka & C.G. Joshi, 2008. Genetic variability and bottleneck studies in Zalawadi, Gohilwadi and Surti goat breeds of Gujarat (India) using microsatellites. Small Ruminant Research, 77, 58-64.
Jouquand, S., C. Priat, C. Hitte, P. Lauchaume, C. Andre and F. Galibert, 2000. Identification and characterization of a set of 100 triand dinucleotide microsatellites in the canine genome. Animal Genetics, 31, 266-272.
Khan, M.S., M.A. Khan & S. Mahmood, 2008. Genetic resources and diversity in Pakistani goats. International Journal of Agriculture Biology, 10, 227-231.
Kim, K. S., J. S. Yeo & C.B. Choi, 2002. Genetic diversity of goats from Korea and China using microsatellite analysis. Asian-Australasian. Journal of Animal Sciences, 4, 461-465.
Marikar, F. M. M. T. & M. M. M. Mustafa, 2013. Usefulness of Short Sequence Repeats (SSR) Markers in Goat Genetic Diversity Studies in Asian and African Continent. Turkish Journal of veterinary and animal sciences, 38, 606-611.
Nei, M., 1973. Analysis of gene diversity in subdivided population. Proceedings of the National Academy of Science, 70, 3321- 3323.
Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590.
Pandey, K., R. Sharma, Y. Singh, B.P. Mishra, K.G. Mondal, P. Singh, K. Singh & B.K. Joshi,. 2010. Variation of 18 STR loci in Shahabadi sheep of India. Russian Journal of Genetics, 45, 117-122.
Rout, P. K., M. B. Joshi, A. Mandal, D. Laloe, L. Singh & K. Thangaraj, 2008. Microsatellite-based phylogeny of Indian domestic goats. BMC Genetics, 9, 1-11.
Sambrook, J. & D. W. Russell, 2001. Molecular Cloning: A laboratory Manual, 3rd edition, . Spring Harbor, New York, USA, Cold spring Harbor Laboratory Press, Cold, pp 105-235.
Teneva, A., E. Todorouska, N. Tyufekchiev, L. Kozelov, A. Atanassov, S, Foteva, S. Ralcheva & S. Zlatarev, 2005. Molecular characterization of Bulgaraian livestock genetic resource 1.
Genetic diversity in Bulgarian cattle as revealed by microsatellite markers. Biotechnology Animal Husbandry, 21, 35-41.
Wang, J.A., Z.H. Shi, Z. Liu, H.F. Lu & H.J. Zhou, 2006. Assessment and regional difference of disaster resilience capability in China. Journal of Natural Disasters, 15, 23–32.
Yang, L., S.H. Zhao, K. Li, Z.Z. Peng & G.W. Montgomery, 1999. Determination of genetic relationships among five indigenous Chinese goat breeds with six microsatellite markers. Animal Genetics, 30, 452-455
Yeh, F. C. & R. Yong, 1999. POPGENE version 1.31: Microsoft-based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada.