Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR
Resumen
Increasing bacterial resistance towards traditional/conventional antibiotics is a major global health concern worldwide. Iron oxide nanoparticles (Fe nanoparticles, with average size of 20 nm) have considerable potential as antimicrobial agents in food safety applications due to their structure, surface properties, and stability. The aim of this work was to investigate the antibacterial effects and mechanism of action of iron nanoparticles against the expression of the tetA gene in Tetracycline Resistant Staphylococcus aureus strains by real time PCR. In the cross-sectional study, a total of 60 S. aureus were collected. Antibiotic susceptibility test was performed on the muller hinton agar according to the Clinical and Laboratory Standards Institute (CLSI). Then all strains were evaluated for tetA, tetB, tetC and tetD genes by multiplex-PCR method. In-vitro activity of iron oxide nanoparticles was evaluated against all resistant strains by microbroth dilution method. Therefore, the expression of tetA gene was measured in treated with iron oxide nanoparticles and untreated resistant S. aureus strain by Real time PCR. Our results indicated 25 (41.66%) strains resistant to Tetracycline. The prevalence of tetA, tetB, tetC and tetD genes were 5 (8.33%), 2 (2.33%), 20 (33.33%) and 10 (10.67%), respectively. The expression of tetA genes in resistant S. aureus strains treated with Iron oxide nanoparticles was lower than the untreated isolates. Iron oxide nanoparticles have strong antibacterial activity against resistant to Tetracycline S. aureus strains. In addition to, these nanoparticles reduce the expression of antibiotic resistance gene.
Article Details
- Cómo citar
-
ARAB, H., SHOJAEE SADI, B., & AMINI, K. (2018). Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR. Journal of the Hellenic Veterinary Medical Society, 69(2), 973–978. https://doi.org/10.12681/jhvms.18025
- Número
- Vol. 69 Núm. 2 (2018)
- Sección
- Research Articles
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Descargas
Los datos de descargas todavía no están disponibles.
Citas
Aruoja V, Dubourguier H-C, Kasemets K, Kahru A (2009) Toxicity of nanoparticles of CuO, ZnO and TiO 2 to microalgae Pseudokirchneriella subcapitata. Science of the total environment 407(4):1461-8.
Azam A, Ahmed A, Oves M, Khan MS, Memic A (1989) Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains. Int J Nanomedicine 7: 3527-3535.
Bondarenko O, Ivask A, Käkinen A, Kahru A (2012) Sub-toxic effects of CuO nanoparticles on bacteria: kinetics, role of Cu ions and possible mechanisms of action. Environmental pollution.169:81-90.
Chow, Anthony W.; Patten, Valerie; Guze, Lucien B. (1975) Comparative Susceptibility of Anaerobic Bacteria to Minocycline, Doxycycline, and Tetracycline”. Antimicrobial Agents and Chemotherapy 7 (1):46–49.
Hadi M, Shokoohi R, Ebrahimzadeh Namvar A, Karimi M, Solaimany Aminabad M (2011) Antibiotic resistance of isolated bacteria from urban and hospital wastewaters in Hamadan City. Iranian Journal of Health and Environment 4(1):105-14.
Masalha M; et al. (2001) Analysis of Transcription of the Staphylococcus Aureus Aerobic Class Ib and Anaerobic Class III Ribonucleotide Reductase Genes in Response to Oxygen. Journal of Bacteriology. 183 (24): 7260–7272.
Noble W, Virani Z, Cree RG (1992) Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS microbiology letters 93(2):195-8.
Ohira T, Yamamoto O, Iida Y, Nakagawa Z-e (2008) Antibacterial activity of ZnO powder with crystallographic orientation. Journal of Materials Science: Materials in Medicine 19(3):1407-12.
Panáček A, Kvítek L, Prucek R, Kolář M, Večeřová R, Pizúrová N, et al (2006)Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. The Journal of Physical Chemistry B 110(33):248-253.
Soenen SJ, Himmelreich U, Nuytten N, Pisanic TR, Ferrari A, De Cuyper M (2010) Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality. Small 6(19):2136-45.
Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of colloid and interface science 275(1):177-82.
Tiwari HK, Sen MR (2006) Emergence of vancomycin resistant Staphylococcus aureus (VRSA) from a tertiary care hospital from northern part of India. BMC Infectious diseases 6(1):156.
Tawale J, Dey K, Pasricha R, Sood K, Srivastava A (2010) Synthesis and characterization of ZnO tetrapods for optical and antibacterial applications. Thin Solid Films 519(3):1244-7.
Tsiodras S, Gold H, Sakoulas G, Eliopoulos GM, Wennersten MD, Venkataraman L, Moellering Jr (2001) Linezolid resistance in a clinical isolate of Staphylococcus aureus. The Lancet 358: 207–208.
