Protective Effect of Spirulina against Aspartame Induced Oxidative Stress and Molecular Gene Brain damage

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Publicado: Apr 29, 2022
DOI: https://doi.org/10.12681/jhvms.25509
Hamam Attiya
Department of Biochemistry and food deficiency, Animal Health Research Institute, Dokki, Giza, Egypt
Resumen

Spirulina and lipoic acid has been considered as one of the most effective in ant oxidative stress and anti-inflammatory activity.Aspartame (ASP) is one of the mostwidely used (artificial sweeteners)used in a variety of foods and feeds.This study was conducted to evaluate the possible protective effect of Spirulina and alpha-lipoic acid against aspartame induced oxidative stress and brain damage in rabbits. Forty two white male New-Zealand Rabbits were classified into seven equal groups. Group I: (Control group) received no drugs. Group II: rabbits administered with aspartame (250 mg/kg b. wt/day). Group III: rabbits received alpha- lipoic acid (100 mg/kg b. wt/day). Group IV: rabbits received spirulina platensis (1500 mg/kg. b. wt/day). Group V: rabbits received aspartame (250 mg mg/kg b. wt) and treated with alpha- lipoic acid (100 mg/kg b. wt). Group VI: rabbits received aspartame (250 mg mg/kg b.wt) and treated with spirulina platensis (1500 mg/kg b. wt). Group VII: rabbits received aspartame (250 mg mg/kg b.wt) and treated daily with alpha- lipoic acid (100 mg/kg b. wt) and spirulina platensis (1500 mg/kg b. wt) for 8 weeks. At the end of experiment brain tissue were isolated and analyzed for determination of L-malondialdehyde  (L-MDA), Catalase (CAT) and reduced glutathione (GSH) in addition to Anti-inflammatory cytokines: Interlukin-10 (IL-10),Activator Protein-1(AP-1),Bax gene expression and DNA damage. The obtained results showed a significant  up-regulation of  Activator Protein-1(AP-1),Bax gene expression level and a significant  down-regulation of Interlukin-10 (IL-10) and marked increase in L-MDA  and DNA damage that was indicated by an increase in tail length and tail DNA percent in brain tissue of aspartame treated rabbits. However, brain CAT activity andGSH concentration were markedly decreased when compared with control group.Coad ministration of spirulinaand alpha lipoic acid protect aspartame induced brain damage in rabbits caused a significant improvement of all previous parameters and attenuate DNA changes. Conclusively, spirulina platensis and alpha-lipoic acid  exerts a protective effect against DNA damage and oxidative stress in brain of aspartameusage through free radical scavenging and anti-inflammatory activities as well as regenerating endogenous antioxidants defense system mechanisms.

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Abdel-Daim MM, Abuzead SM, Halawa SM (2013) Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. PLoS ONE 8(9):e72991.
Abdelkhalek NK, Ghazy EW, Abdel-Daim MM (2015) Pharmacodynamic interaction of Spirulina platensis and deltamethrin in freshwater fish Nile tilapia, Oreochromisniloticus: impact on lipid peroxidation and oxidative stress. Environ Sci Pollut Res Int 22(4):3023–3031.
Abdel-Salam OM, Salem, NA, El-Shamarka ME, Hussein JS, Ahmed NA, El-Nagar, ME (2012) Studies on the effects of aspartame on memory and oxidative stress in brain of mice. Eur Rev Med Pharmacol Sci 16(15): 2092-101.
Ardalan MR, Tabibi H, Attari VE, Mahdavi, AM (2017) Nephrotoxic Effect of Aspartame as an Artificial Sweetener A Brief Review 11(5):339–43.
Ashok I, Sheeladevi R (2014) Biochemical responses and mitochondrial mediated activation of apoptosis on long-term effect of aspartame in rat brain. Redox Biol 2:820-831.
Barceloux DG, Bond GR, Krenzelok EP, Cooper H, Vale JA (2002)American Academy of Clinical Toxicology practice guidelines on the treatment of methanol poisoning. Clin Toxicol, 40, 415.
Beg A, Finco TS, Nantermet PV, Baldwin J (1993) Tumor necrosis factor and interleukin-1 lead to phosphorylation and loss of I
Bermejo P, Piero E, Villar M (2008) Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis. Food Chem 110(2):436–445.
Beutler E, Duro O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61, 882.
Bhat VB, Madyastha KM (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: protection against oxidative damage to DNA. Biochem Biophys Res Common 285:262–266.
Blaylock RL, (2002) Aspartame, Monosodium glutamate and other Excitotoxins and the Hypothalamus, From: "Russell MD, Borlongan, CV, Kanning K, Poulos, TB, Cahill, DW, Sanberg PR, Free radical damage and oxidative stress in Huntington's disease. J Fla Med Assoc 83: 335-341,.
Boldin MP, Varfolomeev, EE, Pancer Z, Mett IL, Camonis JH, Wallach D (1995) A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death. J Biol Chem 270(14):7795-8.
Butterfield DA, Stadtman ER (1997) Protein oxidation processes in aging brain. Adv Cell Aging Gerontol, 2:161.
Čabarkapa A, Živković L, Žukovec D, Djelić N, Bajić V, Dekanski D, Spremo-Potparević, B (2014) Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. Toxicol In Vitro 28:451-456.
Chaudiere J, Ferrari-iliou R (1999) Intracellular antioxidants: from chemical to biochemical mechanisms. Food Chem Toxicol 37:949.
Colla LM, Muccillo-Baisch AL, Costa JAV (2008)Spirulina platensis Effects on the Levels of Total Cholesterol, HDL and Triacylglycerols in Rabbits Fed with a Hypercholesterolemic Diet. Brazilian Arch Biol and Technol 51:405–411.
Coulombe RA, Sharma RS (1986) Neurobiochemical alterations induced by the artificial sweetener aspartame (Nutrasweet). Toxicol Appl Pharmacol, 83:79.
Davoli E Cappellini L, Airoldi L, Fanelli R (1986) Serum methanol concentration in rats and in men after a single dose of aspartame. Food Chem Toxicol 24:187–189.
DowlatiY, Herrmann N, Swardfager W, Liu H, Sham L, Reim EK (2010) A Meta-Analysis of Cytokines in Major Depression. Biological Psychiatry 67(5):446-57.
Eder E, Wacker M, Lutz U, Nair J, Fang X, Batsch H, Beland FA, Schlatter J, Lutz WK (2006) Oxidative stress related DNA adducts in theliver of female rats fed with sunflower, grapeseed, olive or coconut oil supplemented diets. Chem Biol. Interact 159: 81–89.
EFSA (2006) EFSA assesses new aspartame study and reconfirms its safety. European Food Safety Authority. European Ramazzini Foundation, Bologna, Italy. 196, 117.
El-Haliem N, Mohamed D (2011)The effect of aspartame on the histological structure of the liver and renal cortex of adult male albino rat and the possible protective effect of Pimpinellaanisum oil. Egypt J Histol 34: 715-726 .
Gougeon R, Spidel M, Lee K, Field CJ (2004) Canadian diabetes association national nutrition committee technical review: Non-nutritive intense sweeteners in diabetes management. Canadian J Diabetes 28:385.
Halliwell B, Gutteridge JMC (1999) Oxidative stress: adaptation, damage, repair and death. In: Free Radicals in Biology and Medicine, third ed. Oxford University Press, Oxford, , pp. 246-349.
Harper AE (1984) Phenylalanine metabolism, In Stegink LD, Filer LJ Jr eds. Aspartame, physiology and biochemistry. Marcel Dekker, Inc., pp. 77-109.
Hassan A M, Abdel-Aziem SH Abdel-Wahhab MA (2012) Modulation of DNA damage and alteration of gene expression during aflatoxicosis via dietary supplementation of Spirulina (Arthrospira) and Whey protein concentrate. Ecotoxicol Environ Saf 79:294–300.
Ho PI, Daniela Ortiz Rogers E, Shea T (2003) Multipe aspects of Homocysteine nurotoxicty, kinase hyperactivation and DNA damge, J Neurosci Res 70:694-702.
Humphries P, Pretorius E, EH, Naud (2008) Direct and indirect cellular effects of aspartame on the brain. Eur J Clin Nutr 62:451-462.
Iman M, Mourad (2011)Effectof aspartame on some oxidative stress parameters in liver and kidney of rats. Afr J Pharm Pharmacol 5(6):678 -862.
Ismail MF, Ali DA, FernandoA, Abdraboh ME, Gaur RL, IbrahimWM, Raj, MH G, Ouhtit A (2009)Chemoprevention of Rat Liver Toxicity and Carcinogenesis by Spirulina. Intern J Biol Sci 5 (4): 377-387.
Juarez-Oropeza MA, Mascher D, Torres-Duran PV, Farias JM, Paredes-Carbajal MC (2009) Effects of Spirulina on vascular reactivity. J Med Food 12(1): 15-20.
Kaji T, Fujiwara Y, Inomata (2002) Repair of wounded monolayers of cultured bovine aortic endothelial cells is inhibited by calcium spirulina, a novel sulfated polysaccharide isolated from Spirulina platensis. Life Sci 70:1841-1848.
Konícˇková R, Vanˇková K, Vaníková J (2014) Anti-cancer effects of blue-green alga Spirulina platensis, a natural source of bilirubin like tetrapyrrolic compounds. Ann Hepatol 13(2):273–283.
Kono Y, Fridorich I (1989) Superoxide radical inhibits catalase. J Biol Chem 257:5751-5754.
Krebs MO (1992) Excitatory amino acids: a new class of neurotransmitters: pharmacology and functional properties. Encephale 18, 271.
Lebda MA, Sadek KM, El-Sayed, Y S (2017) Aspartame and Soft Drink-Mediated Neurotoxicity in Rats: Implication of Oxidative Stress, Apoptotic Signaling Pathways, Electrolytes and Hormonal Levels. Metabolic Brain Disease 32: 1639–1647.
Li R, Ji, W, Pang J (2013) Alpha-lipoic acid ameliorates oxidative stress byincreasing aldehyde dehydrogenase-2 activity in patients with acute coronary syndrome. The Tohoku J Experim Med 229:45-51.
Lin AJ, Zhang X, Chen M, Cao Q (2007) Oxidative stress and DNA damages induced by cadmium accumulation. J Environ Sci 19:596-602.
Magnuson BA, Burdock GA, Doull J, Kroes RM, Marsh G.M, Pariza MW, Spencer PS, Waddell WJ, Walker R, Williams GM (2007) Aspartame: A safety evaluation based on current use level, regulation, and toxicological and epidemiological studies. Critical Rev Toxicol 629,37.
Mbazima VG, Mokgotho MP, February F, Rees DJG, Mampuru LJM (2008) Alteration ofBax-to-Bcl - 2 ratio modulates the anticancer activity of methanolicextract of ommelinabenghalensis (Commelinaceae) in Jurkat T cells. Afr J Biotech the Amer Assoc Neurosci Nurses 37 (2):102 – 107.
Nishikimi M, Roa NA, Yogi K (1972) Measurement of superoxide dismutase Biochem Bioph Res Common 46:849 – 854.
Ohkawa, H., Ohishi, W., Yagi, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351-8.
Pardridge WM (1979) Regulation of amino acid availability to brain: selective control mechanisms for glutamate, in LJ Filer et al. eds., Glutamic acid, Advances in Biochemistry and Physiology, Raven Press, New York, pp. 125-137.
Park HJ, Lee YJ, Ryu HK (2008)A randomized double-blind, placebo-controlled study to establish the effects of spirulina in elderly Koreans. Ann Nutr Metab 52(4):322–328.
Ranney JA, Opperman E, Muldoon FG, McMahon (1976) Comparative meta-bolism of aspartame in experimental animals and humans, J. Toxicol. Environ. Health 2 (2) 441e451.
Saad A, Khan FA, Hayee A, Nazir MS (2014) Areview on potential toxicity of artificial sweeteners vs safety of stevia :A Health natural biology. Agri Healthcare 4(15):1-12.
Saber TM, Elgam SA, Ali HA, Saleh AA (2015) Protective effect of Spirulina platensis against aluminium-induced nephrotoxicity and DNA damage in rats, Toxic & Environ Chem 97:(8)1113-1123.
SAS (2008) Statistical analysis system : User‘s guide v. 9.2, Inst. Inc., Cary N.C., USA.
Sharoud MNM (2015) Protective effect of Spirulina against paracetamolinduced hepatic injury in rats. J Exper Biol Agric Sci 3(1):44-53.
Shen Q, Uray IP, Li Y, Krisko TI, Strecker TE, Kim HT, Brown PH (2008)The AP-1 transcription factor regulates breast cancer cell growth via cyclins and E2F factors. Oncogene 27:366–377.
Shirpoor A, Minassian S, Salami S, Khadem-Ansari HM, Yeghiazaryan M (2008) Alpha - Lipoic Acid Decreases DNA Damage and Oxidative Stress Induced by Alcohol in the Developing Hippocampus and Cerebellum of Rat. Cell Physiol Biochem 22:769-776
Soffritti M, Belpoggi F, DegliEsposti D, Lambertini L, Tibaldi E, Rigano A (2006) First experimental demonstration of the multi. Health Perspect 115: 1293–1297.
Soffritti M, Belpoggi F, Manservigi M, Tibaldi E, Lauriola M, Falcioni L, Bua L (2010) Aspartame administered in feed, beginning prenatally through life span, induces cancers of the liver and lung in male Swiss mice. Am J Ind Med 53(12):1197-206.
Soffritti M, Belpoggi F, Tibaldi E, Esposti, DD, Lauriola M (2007) Life – span exposure to low doses of aspartame beginning during prenatal life increases cancer effects in rats. Environmental Health Perspectives 115 (9): 1293 – 1297.
Sohal RS, Mockett RJ, Orr, WC (2002) Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Radic Biol Med 33(5):575-86.
Sun JP, Han Q, Zhang XQ (2014) Investigations on the degradation of aspartame using high-performance liquid chromatography/tandem mass spectrometry. Chin Chem Lett 25:1259–1264.
Thomas A, Giesler T, White E(2000) p53 mediates bcl2 phosphorylation and apoptosi s via activation of the Cdc42/JNK1 pathway. Oncogene 19(46): 5259– 5269.
Tilsonh A, Hongjs Sobotkatj (1991) High doses of as-partame have no effects on sensorimotor functionor learning and memory in rats. Neurotox & Teratol 13: 27-35.
Winiarska K Malinska D, Szymanski K, Dudziak M, Bryla J (2008) Lipoic acid ameliorates oxidative stress and renal injury in alloxan diabetes rabbits. Bioch 90: 450459.
Ying L, Zhao Y, Yu W, Jiang S (2004)Scavenging ability on ROS of alpha-lipoic aicd (ALA). Food Chem 84:563-567.
Yoshikawa T, Naito Y (2002) What is oxidative stress. JMAJ45(7):271–276.
Findikli Z, Turkoglu S (2014) Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay. J Toxico Environm Health Sci 6(8): 147-153.