Correlation of a quantitative and a semi-quantitative method for proteinuria detection in chronic kidney disease in dogs

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DOI: https://doi.org/10.12681/jhvms.27529
R.D. BAKA
Diagnostic Laboratory, Faculty of Veterinary Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
C.K. KOUTINAS
Clinic of Companion Animals, Faculty of Veterinary Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
L.V. ATHANASIOU
Department of Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece
Z.S. Polizopoulou
Diagnostic Laboratory, Faculty of Veterinary Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
Resumen

Proteinuria can arise in various physiologic and pathologic conditions. Persistent proteinuria without any abnormalities detected in urine sediment is indicative of chronic kidney disease and has great diagnostic value as it is used for the categorization of the patient on IRIS (International Renal Interest Society) staging system. There are several techniques for urine protein measurement including the semi-quantitative/qualitative (urine dipstick, sulfosalicylic acid turbidimetric test and Heller’s reaction test) and the quantitative tests (urine protein to creatinine ratio (UPC) and microalbuminuria assay).The purpose of this study was to correlate the semi-quantitative Heller’s reaction test for proteinuria detection, with the UPC in urine samples from 89 dogs with chronic kidney disease. The non-parametric Spearman’s correlation coefficient was used to correlate Heller’s reaction test with UPC in urine samples from dogs with chronic kidney disease in proteinuria detection. Correlation analysis revealed a statistically significant positive and moderate correlation between the Heller’s reaction test and UPC (r(89)=0.510, p<0.0001) which was slightly improved when USG>1010 (r(72)=0.541, p<0.0001)(urine specific gravity).Heller’s reaction test might be a useful alternative to detect proteinuria when UPC is not available in the clinical setting, however it cannot be used interchangeably with UPC for the IRIS sub-staging of chronic kidney disease (CKD).

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Adams l, Polzin D, Osborne C, O’ Brien T (1992) Correlation of urine protein/creatinine ratio and twenty-four-hour urinary protein excretion in normal cats and cats with surgically induced chronic renal failure. J Vet Intern Med6: 36-40.
Athanasiou L, Kasabalis D, Mpairamoglou S, Katsogiannou E, Apostolidis K, Soubasis N, Tsokana C, Saridomichelakis M (2014) Evaluation of Heller’s reaction and/or urine dipstick for the detection of proteinuria in dogs. In: Procceeding of the 16th ESVCP Annual Congress,
Milan, Italy.
Beatrice L, Nizi F, Callegari D, Paltrinieri S, ZiniE., D’ Ippolito P, Zatelli A (2010) Comparison of urine protein-to-creatinine ratio in urine samples collected by cystocentesis versus free catch in dogs. J Am Vet Med Assoc236:1221-1224.
Burton C, Harris K (1996) The role of proteinuria in the progression of chronic renal failure. Am J Kidney Dis27:765-775.
Grauer G, Moore L, Smith A, Jensen W (2004) Comparison of conventional urine protein test strip method and a quantitative ELISA for the detection of canine and feline albuminuria. J Vet Intern Med18:418-419.
Garner B, Wiedmeyer C (2007) Comparison of semi-quantitative pointof-care assay for the detection of canine microalbuminuria with routine semiquantitative methods for proteinuria. Vet Clin Pathol 36:240-244.
Grauer G (2011) Proteinuria: Measurement and interpretation. Top Companion Anim Med 26: 121-127.
Herley L, Langston C (2012) Proteinuria in dogs and cats. Can Vet J 53:631-638.
International Renal Interest Society Staging of CKD, 2019 http://www.iris.com/pdf/IRIS_Staging_of_CKD_Modified_2019.pdf [accessed 6 March 2020]
Jacob F, Polzin D, Osborne C, Neaton J, Kirk C, Allen T, Swanson L (2005) Evaluation of the association between initial proteinuria and morbidity rate or death in dogs with naturally occurring chronic renal failure. J Am Vet Med Assoc 226: 393-400.
Le Vine D, Zhang D, Harris T, Vaden S (2010) The use of pooled VS serial urine samples to measure urine protein: creatinine ratio. Vet Clin Pathol 39: 53-56.
Lyon S, Sanderson M, Vaden S, Lappin M, Jensen W, Grauer G (2010) Comparison of urine dipstick, sulfosalicylic acid, urine protein-to-creatinine ratio, and species-specific ELISA methods for detection of albumin in urine samples of cats and dogs. J Am Vet Med Assoc 236:874-879.
Mardell EJ, Sparkes AH (2006) Comparison of a semiquantitative pointof-care assay for the detection of canine microalbuminuria with routine semiquantitative methods for proteinuria. Vet Clin Pathol 36: 240-244.
Medaille C, Brien-Marshal A (2008) Analyse d’ urine. In: Guide pratique des analyses biologiquesvétérinaires.1st ed, Med com, Paris: pp 288-304.
Monroe W, Davenport D, Saunders G (1989) Twenty-four hour urinary protein loss in healthy cats and the urinary protein-creatinine ratio as an estimate. Am J Vet Res 50: 1906-1909.
R core team (2013) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
RemuzziG (1999) Nephropathic nature of proteinuria. Curr Opin Nephrol Hypertens 8: 655-663.
Sink C, Weinstein N (2012) Proteinuria. In: Practical Veterinary Urinalysis. 1st ed, Wiley- Blackwell, Singapore: pp 113-132.
Stockham SL, Scott MA (2008) Urinary system. In: Fundamentals of Veterinary Clinical Pathology. 2nd ed, Blackwell Publishing, Ames: pp 415-494.
Strasinger SK, DiLorenzo MS (2008) Chemical examination of urine. In: Urinalysis and Body Fluids. 5th ed, Davis Company, Philadelphia: pp 53-80.
Walls J (2001) Relationship between proteinuria and progressive renal disease. Am J Kidney Dis 37:513-516.
Welles E, Whatley E, Hall A, Wright J (2006) Comparison of Multistix PRO dipsticks with other biochemical assays for determining urine protein (UP), urine creatinine (UC), and UP:UC ratio in dogs and cats. Vet Clin Pathol 35: 31-36.
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