Assessment anti-granuloma activity of Alpha-lipoic acid in chicks’ models

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Publicado: Apr 12, 2023
Actualizado: 2023-04-12
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2023-04-12 (2)
DOI: https://doi.org/10.12681/jhvms.29033
MR Abdul-Ghani
University of Mosul · College of Veterinary Medicine, Iraq
https://orcid.org/0000-0003-1294-3674
AS Naser
Resumen

Background: Alpha-lipoic acid is an anti-inflammatory and antioxidant agent that has free radical scavenging characteristics as well as better effects on the recycling of other cellular antioxidants.

Aim: The present study was made to assess the anti-granuloma of Alpha-lipoic acid by the cotton pellet granuloma method.

Method: The chicks weighing around 100-150 g were selected, the Alpha-lipoic acid was administered at the following dose of 20, 40 & 80 mg/kg body weight respectively after insertion of cotton pellets into the groin region of chicks.

Results: By using the up-and-down technique, it was found that the Median Lethal Dose (LD50) of the Alpha-lipoic acid was 451.8mg/kg, IP.  Alpha-lipoic acid at 20, 40, and 80 mg/kg per body weight daily for consecutive seven days revealed that the ALA shows dose-dependent inhibition of weight of both wet and dry cotton pellets. The inhibition percentage for wet cotton pellets was 5, 11, and 14 respectively whereas the inhibition percentage for dry cotton pellets was 9, 15, and 25 respectively.  

Conclusion: We conclude that Alpha-lipoic acid has notable anti-granuloma activity as compared to control in animal models of chronic inflammation. Additional studies must be done to find the molecular role of Alpha-lipoic acid as anti-granuloma remedies

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Abraham GJS, Agshikar NV (1972). Anti-inflammatory activity of an essential oil from Zanthoxylum budrunga. Pharmacology, 7(2), 109–114.
Alatrushi AN, Naser AS (2021). The Safety Profile of the Anesthetic Effect of Alfaxalone and Its Interaction with Xylazine and Ketamine in Chick’s Model (). Macedonian Veterinary Review.
Andrea Moura F, Queiroz de Andrade K,Celia Farias dos Santos J, Oliveira Fonseca Goulart M (2015) Lipoic acid: its antioxidant and anti-inflammatory role and clinical applications. Current Topics in Medicinal Chemistry, 15(5), 458–483.
Bulut N E, Özkan E, Ekinci O, Dulundu E , Topaloğlu Ü , Şehirli A Ö, Şener G (2011) Beneficial effects of alpha lipoic acid on cerulein-induced experimental acute pancreatitis in rats. Ulus Travma Acil Cerrahi Derg, 17(5), 383–389.
Çakir T , Polat C , Baştürk A , Gül, M , Aslaner A , Durgut H , Sabuncuoglu MZ (2015) The effect of alpha lipoic acid on rat kidneys in methotrexate induced oxidative injury. Eur Rev Med Pharmacol Sci, 19(11), 2132–2139.
Ching FP, Omogbai EKI, Okpo SO, Ozolua RI (2009) Antiinflammatory activity of aqueous extract of Stereospermum kunthianum (Cham, Sandrine Petit) stem bark in rats. Indian Journal of Pharmaceutical Sciences, 71(1), 106.
Dixon WJ (1980) Efficient analysis of experimental observations. Annual Review of Pharmacology and Toxicology, 20(1), 441–462.
Gomaa AMS, Abd El-Mottaleb NA, Aamer HA (2018) Antioxidant and anti-inflammatory activities of alpha lipoic acid protect against indomethacin-induced gastric ulcer in rats. Biomedicine & Pharmacotherapy, 101, 188–194.
Gomes MB, Negrato CA (2014) Alpha-lipoic acid as a pleiotropic compound with potential therapeutic use in diabetes and other chronic diseases. Diabetology & Metabolic Syndrome, 6(1), 1–18.
Ismail TS, Gopalakrishnan S, Begum VH, Elango V (1997) Anti-inflammatory activity of Salacia oblonga Wall. and Azima tetracantha Lam. Journal of Ethnopharmacology, 56(2), 145–152.
Kapsokefalou A, Heuser C, Abken H, Rappl G, Röβler M ,Ugurel S,Reinhold U (2001) Dermal fibroblasts sustain proliferation of activated T cells via membrane-bound interleukin-15 upon long-term stimulation with tumor necrosis factor-α. Journal of Investigative Dermatology, 116(1), 102–109.
Naser AS, Albadrany YM (2021) The neurobehavioral effects of flumazenil in chicks. Iragi journal of veterinary scinces , 25, 27.
Olajide OA, Makinde JM, Awe SO (1999) Effects of the aqueous extract of Bridelia ferruginea stem bark on carrageenan-induced oedema and granuloma tissue formation in rats and mice. Journal of Ethnopharmacology, 66(1), 113–117.
Perera J, Tan J. H, Jeevathayaparan S, Chakravarthi S, Haleagrahara N (2011) Neuroprotective effects of alpha lipoic acid on haloperidol-induced oxidative stress in the rat brain. Cell & Bioscience, 1(1), 1–6.
Román-Pintos LM, Villegas-Rivera G, Rodríguez-Carrizalez AD, Miranda-Díaz A G,Cardona-Muñoz EG (2016) Diabetic polyneuropathy in type 2 diabetes mellitus: inflammation, oxidative stress, and mitochondrial function. Journal of Diabetes Research, 2016.
Shen H.-H , Lam K.-K , Cheng P -Y , Kung C -W , Chen S -Y , Lin P -C, Lee Y -M (2015) Alpha-lipoic acid prevents endotoxic shock and multiple organ dysfunction syndrome induced by endotoxemia in rats. Shock, 43(4), 405–411.
Subash A , Veeraraghavan G , Sali VK , Bhardwaj M, Vasanthi H R (2016) Attenuation of inflammation by marine algae Turbinaria ornata in cotton pellet induced granuloma mediated by fucoidan like sulphated polysaccharide. Carbohydrate Polymers, 151, 1261–1268.
Tastekin N, Aydogdu N , Dokmeci D , Usta U , Birtane M , Erbas H , Ture M (2007) Protective effects of L-carnitine and alpha-lipoic acid in rats with adjuvant arthritis. Pharmacological Research, 56(4), 303–310.
Tibullo D, Volti GL, Giallongo C, Grasso S, Tomassoni D, Anfuso C D, Bramanti V (2017) Biochemical and clinical relevance of alpha lipoic acid: antioxidant and anti-inflammatory activity, molecular pathways and therapeutic potential. Inflammation Research, 66(11), 947–959.
Uzkeser H , Cadirci E , Halici Z , Odabasoglu F, Polat B, Yuksel TN, Atalay F (2012) Anti-inflammatory and antinociceptive effects of salbutamol on acute and chronic models of inflammation in rats: involvement of an antioxidant mechanism. Mediators of Inflammation, 2012.