Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020


Publicado: Jan 18, 2024
Actualizado: 2024-01-18
BS Tokgoz
EA Tokgoz
MA Sözmen
O Avci
AE Ütük
Resumen

The aim of this study is to investigate the presence of BEF disease in the blood, sera and tissue samples sent to the Viral Diagnosis Laboratory of Adana Veterinary Control Institute with suspected BEF in 2020 from different provinces of Turkey as well as to perform characterization and phylogenetic analysis of cases tested positive with molecular methods. For this purpose, 79 defibrinated blood and 2 tissue samples as well as 168 sera samples obtained from cattle varying in breed, age, and sex, were examined by using Real Time RT-PCR and Blocking ELISA, respectively. Two new degenerate primers amplifying 956 bp of the protein G (AVKEF_AATGTTCCNGTGAATTGTGGAG and AVKER_TGCATAATCYCTTCCTGGTCT) were designed and the phylogenetic analysis of the samples tested positive with RT-PCR was performed. As a result of the examinations conducted, 64.20% (52/81) of the defibrinated blood and tissue samples, and 69.04% (116/168) of the sera samples were diagnosed as positive. Through VERO cell culture, the virus was isolated from the tissue samples of 2 animals in Adana and Sanliurfa provinces, which were positive. The phylogenetic analysis revealed that the virus circulated in Turkey during the 2020 pandemic belongs to the Middle East lineage. We concluded that monitoring the disease and identifying the lineages of the virus that have caused pandemic are significant in the selection of the proper vaccine and the disease control.

Article Details
  • Sección
  • Research Articles
Descargas
Los datos de descargas todavía no están disponibles.
Citas
Abayli H,Tonbak S,Azkur A, Bulut, H (2017) Complete genome analysis
of highly pathogenic bovine ephemeral fever virus isolated in Turkey
in 2012. Arch Virol 162(10):3233-3238.
Abed Elnaby SS, Rateb MH (2019) Detection of specific antibody and
some biochemical change in bovine ephemeral fever virus in ElWady El-Gedid Governorate. Animal Health Res J 7(3): 1-11.
Albayrak H,Ozan E (2010)Seroprevalence of some Arboviral infections
transported blood sucking insects in ruminants and equids in Middle
Blacksearegion in Turkey.Kafkas Univ Vet Fak Derg 16(1): 33-36.
Alkan F, Albayrak H,Timurkan MO, Ozan E, Coskun N (2017) Assessment of the molecular epidemiology of bovine ephemeral fever in
Turkey. Vet Arh 87(6): 665-675.
Aziz-Boaron O, Brettschneider S, King R, GelmanB, Klement E (2013)
Seroprevalence of Bovine Ephemeral Fever Virus in domesticated and wildlife species during epidemic and inter-epidemic periods
(2000-2009) in Israel. Transbound Emerg Dis 62:183-187.
Bakhshesh B, Abdollahi D (2015) Bovine Ephemeral Fever in Iran: Diagnosis, isolation and molecular characterization. J Arthropod-Borne
Dis 9(2): 195-203.
Chaisirirat T, Sangthong P, Arunvipas P, Petcharat N, Thangthamniyom
N, Chumsing W, Lekcharoensuk P (2018) Molecular characterization
of bovine ephemeral fever virus in Thailand between 2013 and 2017.
Vet Microbiol 227:1-7.
Clustal Omega Program. http://www.clustal.org/omega/. [Accessed:
02.2022].
DegheidyNSh, Hassan HY, El-Sanousi AA, Salem SA, Beshir E,El-Sadawy HA (2011) Resent trials for diagnosis of Bovine Ephemeral Fever in Egypt. International Congressof the International Society for
Animal Hygene. Conference Animal Hygene and Sustainable Livestock Production,July,Vienna, Austria.
Dorey-Robinson DLW, Fernandezde Marco M, Hernandez-Triana LM,
Folly AJ, McElhinney LM, Stokes JE, Sanders C, Carpenter S, Fooks
AR, Zalesky O, Gelman B,Erster O,Johnson N (2019) Complete genome sequence of a bovine ephemeral fever virus isolate from Israel.
MicrobiolResourAnnounc 8(41) e00822-19.
Erganiş O, Sayın Z,Sakmanoğlu A,Yavru S, Avcı O,Yanık B, Ertürk
A,Ozmen M (2013) Üçgünhastalığıiçinaşıgeliştirmesitrivalanbefaşısıhazırlanmasıilegüvenlikveetkinlikçalışmaları. İnfovet 141-163.
Erol N, Koç BT, Gur S,Caglav YO, Tan MT (2015) A Serological Investigation for Bovine Ephemeral Virus Infection in Aydın and Muğla
Provinces.Kocatepe Vet J 8(2): 41-45.
Felsenstein J (1985) Confidence limits on phylogenies: An approach using
the bootstrap. J Mol Evol 39:783-791.
Geneious Prime 2021.0.3.https://www.geneious.com.[Accessed:
02.2022].
Girgin H,Yonguç AD,Akçora A,Aksak E (1986) The first outbreak of Bovine Ephemeral Fever in Turkey. Etlik Vet Mikrob Ens Derg 5: 10-12.
Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A,Jermiin LS
(2017) ModelFinder: fast model selection for accurate phylogenetic
estimates. Nat Methods 14: 587-589.
Karaoglu T,Ozgunluk I, Demir B,Ozkul A, Burgu I (2007) Seroprevalance
of culicoides-borne disease in cattle in European Turkey. Ankara
Univ Vet Fak Derg 54: 121-125.
Karayel-Hacioglu I, Yelken SD, Vezir Y, Unal N, Alkan F (2021) Isolation
and genetic characterization of bovine ephemeral fever virus from
epidemic-2020 in Turkey. Trop Anim Health Prod 53(2):276.
Kirkland PD(2002) Akabane and Bovine Ephemeral Fever Virus infections. Vet Clin Food Anim 18: 501-514.
Lee F (2019) Bovine Ephemeral Fever in Asia: Recent status and research
gaps. Viruses 11(5): 412.
Liu D, Li K, Zhang L, Lan Y, Wang X, Zhang H, Wang L, Gui R, Han Z,
Jang W,Sizhu S, Li J (2017) Seroprevalence investigation of bovine
ephemeral fever in yaks in Tibetan Plateau of China from 2012 to
Trop Anim Health Prod 49(1): 227-230.
Mahmoud A (2012) Comparative Sequence Analysis and Epitope Prediction of the Partial Glycoprotein G Gene of Bovine Ephemeral Fever
Virus Isolate From Egypt. Egyptian JVirol 9: 49-60.
Mlingo TAM,Nthangeni BM, Mokoena NB (2021) Genome sequence of
Bovine Ephemeral fever virus vaccine strain of South African origin.
Vet Med Sci 7:1611-1615.
Nandi S, Negi BS (1999) Bovine Ephemeral Fever: A Review. CompImmunMicrobiol Infect Dis 22: 81-91.
National Center for Biotechnology.http://www.ncbi.nlm.nih.gov.[Accessed: 11.02.2022].
Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ(2015) IQ-TREE: A
fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol Biol Evol 32:268-274.
Oguzoglu TC, Erturk A, Cizmeci SG, Koc BT,Akca Y(2015) a report on
bovine ephemeral fever virus in Turkey: antigenic variations of different strains of EFV in the 1985 and 2012 Outbreaks using partial
glycoprotein gene sequences. Transbound Emerg Dis 62(9): 66-70.
OIE (2016) http://www.cfsph.iastate.edu/Factsheets/pdfs/bovine_ ephemeral_fever. Bovine Ephemeral Fever.[Accessed: 10.02.2022].
html.[Accessed: 11.02.2022].
Omar R, Schalkwyk AV,Carulei O, Heath L, Douglass N, Williamson AL
(2020) South African Bovine Ephemeral Fever Virus glycoprotein sequences are phylogenetically distinct from those from the rest of the
wold. Arch Virol 165(5): 1207-1210.
Pyasi S,Sahu BP, Sahoo P, Dubey PK, Sahoo N,Byrareddy SN,Nayak
D (2020) Identification and phylogenetic characterization of bovine ephemeral fever virus (BEFV) of Middle Eastern lineage associated with 2018-2019 outbreaks in India. Transbound Emerg
Dis67(5):2226-2232.
Pyasi S,Jonniya NA,FulbabuSk Md, Nayak D, Kar P (2021) Finding potential inhibitors against RNA- dependent RNA polymerase (RdRp)
of bovine ephemeral fever virus (BEFV): an in-silico study. J Biomol
Struct Dyn40(20):10403-10421.
Rezatofighi SE, Mirzadeh K, Mahmoodi F (2022) Molecular characterization and phylogenetic analysis of bovineephemeral fever viruses in
Khuzestan province of Iran from 2018 and 2020. BMC Vet Res 18:19.
Stram Y,Kuznetzova L, Levin A, Yadin H,Rubinstein-Giuni M (2005) A
real-time RT-quanttive(q)PCR for the detection of Bovine Ephermeral Fever Virus. J Virol Methods 130:1-6.
Tonbak S, Berber E, Cabalar M (2013) Detection of bovine ephemeral
fever virus infections by polymerase chain reaction in some regions
of Turkey in 2008.FÜSağBilVetDerg 27(1):35-37.
TrinidadL, Blasdell KR, Joubert DA, Davis SS, Melville L, Kirkland
PD, Coulibaly F, Holmes EC, Walker PJ (2014) Evolution of bovine
ephemeral fever virus in the Australian Episystem. JVirol 88(3):
-1535.
Walker PJ, Byrne KA,Cybinski DH, Doolan DL, Wang Y (1991) Proteins
of Bovine Ephemeral Fever Virus. J Gen Virol 72:67-74.
Walker PJ, Byrne KA, Riding GA, Cowley JA, Wang Y, McWilliam
S(1992) The genome of bovine ephemeral fever rhabdovirus contains
two related glycoprotein genes. Virology 191: 49-61.
Walker PJ(2005) The World of Rhabdoviruses. Fu ZF (editor). Bovine
Ephemeral Fever in Australia and the World.CTMI 292: pp 57-80.
Walker PJ(2008) Bovine Ephemeral Fever Virus. CSIRO Livestock Industries, Geelong, VIC, Australia Elsevier Ltd. Pp: 354-362.
Walker PJ, Klement E (2015) Epidemiology and control of bovine ephemeral fever. Vet Res 46:124.
Yeruham I, Ham MV, Stram Y, Friedgut O, Yadin H,Mumcuoglu KY,
Braverman Y (2010) Epidemiological investigation of bovine ephemeral fever outbreaks in Israel. Vet MedInt 2010:290541.
Zahid M, Durrani AZ, Ijaz M, Muhammad K, Usman M, Husnain M, Kamal N (2019) Seromolecularprevalence of BEFV antibodies, their correlation with physiological biomarkers and identification of risk factors under field conditions. Pakistan J Zool51(1): 205-210.
Zaghawa A,Housawi FMTH, Al-Naeem A, Al-Nakhly H,Kamr A, Toribio R(2016) Risk analysis and seroprevalence of bovine ephemeral fever virus in cattlein the Kingdom of Saudi Arabia. Trop Anim Health
Prod 48:487-492.
Zaghawa AA,Housawi F, Al-Naeem A,Elsify A,Mohammed H (2017)
Bovine Ephemeral Fever epidemics in Kingdom Saudi Arabia: clinical, epidemiological and molecular investigation. J Infect Dev Ctries
(11): 854-860.
Zheng FY, Lin GZ, Qiu CQ, Zhou JZ, Cao XA,Gong XW (2009) Development and application of G1- ELISA for detection of antibodies
against bovine ephemeral fever virus. Res Vet Sci 87(2): 211-212.
Zheng FY, Lin GZ, Qiu CQ, Zhou JZ, Cao XA,Gong XW (2010) Serological detection of bovine ephemeral fever virus using an indirect ELISA based on antigenic site G1 expressed in Pichia pastoris. VetJ
(2): 211-215.
Zheng F, Qiu C (2012) Phylogenetic relationship of the glycoprotein gene of Bovine Ephemeral Fever virus isolated from Mainland China, Taiwan, Japan, Turkey, Israel and Australia. VirolJ 9:268