Beneficial effects of metformin on high fructose-induced ovarian cycle abnormalities and antioxidant activity Effects of metformin on female rats

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Publicado: Jan 16, 2025
DOI: https://doi.org/10.12681/jhvms.33154
MA Demirel
Laboratory Animals Breeding and Experimental Researches Center, Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy Gazi University, Ankara, Turkey
https://orcid.org/0000-0002-7082-8976
E Sumlu
Department of Medical Pharmacology, Faculty of Medicine, KTO Karatay University, Konya, Turkey
MO Uludağ
Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey
F Akar
Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey
Resumen

This study aimed to evaluate the effect of metformin on the regulation of the ovarian cycle through serum antioxidant activities in female rats fructose-induced metabolic syndrome model. Thirty-four, immature female Wistar rats (21 days-old) were randomly divided into five groups (n:4-8/group) control, carboxymethylcellulose (CMC), fructose, metformin, and fructose+metformin groups. The metabolic syndrome model was induced by fructose solution (20% w/v) for 15 weeks. Metformin was administrated by oral gavage in CMC solution for the last final 6 weeks. Vaginal cytology was evaluated before and at the 9th and 15th weeks of the experiment. Total antioxidant status (TAS), total oxidant status (TOS), and paraoxonase (PON)-1 levels were assessed in serum samples. The statistical analysis was performed using a one-way ANOVA test. Results are expressed as the mean ± standard error of the mean (SEM). Metformin restored the regularity of the estrous cycle, which was disturbed by a high fructose diet.  Moreover, dietary high-fructose markedly increased serum TOS and OSI values, but significantly decreased serum TAS and PON-1 levels than the other groups (p < 0.05). Metformin, through its antioxidant activity, may have had a beneficial effect on the impaired ovarian cycle associated with oxidative stress induced by high fructose diet.

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