Comparison of Physiochemical, Biochemical and Antimicrobial Properties of Natural and Artificial Apis mellifera L. Beeswax

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Publicado: Ιουλ 10, 2024
DOI: https://doi.org/10.12681/jhvms.34663
Ö Ertürk
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Ordu University
M Keskin
Bilecik Seyh Edebali University, Vocational School of Health Services, Bilecik, Turkey
https://orcid.org/0000-0001-9365-334X
C Birinci
Department of Chemistry, Faculty of Science, Karadeniz Technical University, Trabzon
S Kolaylı
Department of Chemistry, Faculty of Science, Karadeniz Technical University, Trabzon
Resumen

The interest in bee products is increasing day by day. Beeswax is the honeycomb cells in which bees store their honey. Due to their rich biocompenents, it has both antioxidant and antimicrobial activity. Beeswax could be produced naturally by bees as well as it is commercially available. Commercial beeswax is processed by bees and get ready for honey storage. In this study, the physicochemical properties of commercial and natural beeswax such as water vacuum capacity, oil content, oral secretion and elemental composition were identificated. Then, the beeswax was extracted using different solvents, biochemical and antimicrobial activities of these samples were compared. Natural beeswax was found to have less water absorption capacity, higher oil content (52.79±0.12%) and contained more plant material. Y-acetone extract of beeswax had higher total phenolic content (3.74±0.03 mg GAE/g) and showed a good antioxidant activity (70.23±1.30 µM FeSO4.7H2O/g) than other extracts prepared different solvents. It was clear that both extracts had a good antimicrobial activity. Y-methanol extract was found to be effective on B. subtilis and M. luteus, D-ethanol on E. coli, Y-ethanol on L. monocytogenes, and Y-acetone on P. vulgaris. It was clear that both artificial and natural beeswax could be used in different applications in a wide range food to medicine.

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Citas
Al-Abd NM, Mohamed Nor Z, Mansor M, Azhar F, Hasan MS, Kassim M
(2015). Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract. BMC Complement Altern
Med. 15:385.
Benzie I F, Strain J J (1999) Ferric reducing/antioxidant power assay:
direct measure of total antioxidant activity of biological fluids and
modified version for simultaneous measurement of total antioxidant
power and ascorbic acid concentration. Methods in enzymology 299:
-27.
Bogdanov S (2004). Beeswax: Quality issues today. Bee World, 85(3):
-50.
Bogdanov, S. (2009). Beeswax: Production, properties composition and
control. Beeswax Book. (Chapter 2) https://doi.org/Switzerland (Bee
Product Science. Muehlethurnen, Switzerland) .
Cuce PM, Kolayli S, Cuce E (2020). Enhanced performance figures of
solar cookers through latent heat storage and low-cost booster reflectors. International Journal of Low-Carbon Technologies 15(3): 427-
Curtis TR, Apante Y, Stamp NE (2005). Nest paper absorbency, tougness
and protein concentration of a native vs. invasive social wasp. J Chem
Ecol 31:1089-1100.
de Oliveira AM, Conserva LM, de Souza Ferro JN, de Almeida Brito F,
Lyra Lemos RP, Barreto E (2012). Antinociceptive and anti-inflammatory effects of octacosanol from the leaves of Sabicea grisea var.
grisea in mice. Int J Mol Sci. 13(2):1598-611.
Erturk O (2017). Determination of some structural features of the nest
paper materials of Dolichovespula saxonica Fabricius, 1793 (Hymenoptera: Vespinae) in Turkey. Entomol Res 47:286-294.
Eteraf-Oskouei T, Najafi M (2013). Traditional and modern uses of natural honey in human diseases: A review. Iran. J. Basic Med. Sci. 16:
-742.
Everlyne IM, Sangilimuthu AY, Darsini DTP (2015). Spectral analyses
of the bioactive compounds present in the ethanolic leaf extract of
Strobilanthes kunthiana (Nees) T. Anderson ex. Benth. Adv Bio Res.
(3): 65-71.
Felicioli A, Cilia G, Mancini S, Turchi B, Galaverna G, Cirlini M, ... Fratini F (2019). In vitro antibacterial activity and volatile characterisation
of organic Apis mellifera ligustica (Spinola, 1906) beeswax ethanol
extracts. Food Bioscience 29: 102-109.
Fratini F, Cilia G, Turchi B, Felicioli A (2016). Beeswax: A minireview of
its antimicrobial activity and its application in medicine. Asian Pacific Journal of Tropical Medicine 9(9): 839-843.
Hepburn HR, Bernard RTF, Davidson BC, Muller WJ, Lloyd P, Kurstjens
SP, Vincent SL (1991). Synthesis and secretion of beeswax in honeybees. Apidologie 22(1): 21-36.
Inouye S, Takizawa T, Yamaguchi H (2001). Antibacterial activity of
essential oils and their major constituents against respiratory tract
pathogens by gaseous contact. Journal of Antimicrobial Chemotherapy 47(5): 565-573.
Jiang MH, Yang L, Zhu L, Piao JH, Jiang JG (2011). Comparative GC/
MS analysis of essential oils extracted by 3 methods from the bud of
Citrus aurantium L. var. amara Engl. Journal of food science 76(9):
-1225.
Ka´caniová M, Vukovi´c N, Chlebo R, Hašˇcík P, Rovná K, Cubon J,
Dzugan M, Pasternakiewicz A (2012). The antimicrobial activity of
honey, bee pollen loads and beeswax from Slovakia. Arch. Biol. Sci.
Bel. 64: 927-934.
Kaur J, Jawandha SK, Gill PPS, Grewal SK, Singh H, Adhikary T (2023)
Beeswax enriched gibberellic acid coatings preserve antioxidant
properties and quality of lemon fruit under cold storage condition. Acta Physiologiae Plantarum 45(7): 93.
Kotan A, Cakir F, Dadasoglu T, Aydin R, Cakmakci H, Ozer S, Kordali E,
Mete N, Dikbas (2010). J. Sci. Food Agric. 90: 145.
McLoone P, Warnock M, Fyfe L (2016). Honey: A realistic antimicrobial
for disorders of the skin. J. Microbiol. Immunol. Infect. 49: 161-167.
Nair MKM, Joy J, Vasudevan P, Hinckley L, Hoagland TA, Venkitanarayanan KS (2005). Antibacterial effect of caprylic acid and monocaprylin on major bacterial mastitis pathogens. Journal of Dairy Science 88(10): 3488-3495.
Osae R, Apaliya MT, Alolga RN, Kwaw E, Otu PNY, Akaba S (2022).
Influence of shea butter, bee wax and cassava starch coatings on enzyme inactivation, antioxidant properties, phenolic compounds and
quality retention of tomato (Solanum lycopersicum) fruits. Applied
Food Research 2(1): 100041.
Polat S, Uslu MK, Aygün A, Certel M (2013). The effects of the addition
of corn husk fibre, kaolin and beeswax on cross-linked corn starch
foam. J. Food Eng. 116: 267-276.
Sawicki T, Starowicz M, Kłębukowska L, Hanus P (2022). The profile of
polyphenolic compounds, contents of total phenolics and flavonoids,
and antioxidant and antimicrobial properties of bee products. Molecules 27(4): 1301.
Select Committee on GRAS Substances (SCOGS) Opinion: Beeswax (Yellow or White) (1975). Available online: http://wayback.
archive-it.org/7993/20171031061654/https://www.fda.gov/Food/
IngredientsPackagingLabeling/GRAS/SCOGS/ucm260869.htm (accessed on 22 January 2020).
Sepehr S (2010). The most important medicinal uses of honey, and its side
effects in the book of the Canon by Avicenna, and in the modern medical literature: A comparative study. J. Apiprod. Apimed. Sci. 2: 43.
Svečnjak L, Chesson LA, Gallina A, Maia M, Martinello M, Mutinelli F,
... Waters TA (2019). Standard methods for Apis mellifera beeswax
research. Journal of Apicultural Research 58(2): 1-108.
Tulloch AP (1980). Beeswax—composition and analysis. Bee world 61(2):
-62.
Slinkard K, Singleton VL (1977). Total phenol analysis: Automation and
comparison with manual methods. Am J Enol Viticult 28: 49-55.
Vambe M, Aremu AO, Chukwujekwu JC, Gruz J, Luterová A, Finnie JF,
Van Staden J (2020). Antibacterial, mutagenic properties and chemical characterisation of sugar Bush (Protea caffra Meisn.): A South
African native shrub species. Plants 9(10): 1331.
Yamane S, Kudo K, Tajima T, Nihon’yanagi K, Shinoda M, Saito K, Yamamoto H (1999). Comparison of investment in nest construction by
the foundresses of consubgeneric Polistes wasps, P. (Polistes) riparius and P. (P.) chinensis (Hymenoptera, Vespidae). J Ethol 16:97-104.
Zanoschi C, Ciobanu C, Verbuta A, Frincu D (1991). The efficiency o
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