The evaluation of oxidative stress in lambs with Pestivirus infection

PDF (English)
Publiée : Ιαν 29, 2018
DOI : https://doi.org/10.12681/jhvms.15473
Ο. ASLAN
Faculty of Veterinary Medicine, Erciyes University
Α. GENCAY GOKSU
Faculty of Veterinary Medicine, Erciyes University
Ν. APAYDIN
Faculty of Veterinary Medicine, Erciyes University
Résumé

This study was carried out to measure the plasma malondialdehyde (MDA) level, and erythrocyte glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) activities in lambs with Pestivirus. Sixty lambs aged between 2.5-3 months old were included in the study. Blood and faeces samples were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) test, using pan-Pestivirus primers. Clinical and virological examinations revealed that the numbers of non-infected and Pestivirus -infected animals were 20 (Group 1) and 40 (Group 2), respectively. Plasma MDA levels were found significantly higher in lambs with Pestivirus than that of controls (p<0.05) whilst the erythrocyte GSH-Px, CAT and SOD activities were significantly lower in lambs with Pestivirus (p<0.05) than in the controls. The determination of increased plasma MDA levels and decreased erythrocyte GSH-Px, CAT and SOD activities in the infected lambs confirms that oxidative stress is observed in Pestivirus. The presence of oxidative stress in Pestivirus indicates that the equilibrium between oxidants and antioxidants is shifted towards oxidants in lambs with Pestivirus.

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Balıkcı E, Yildiz A, Gurdogan F (2013) Selected acute phase proteins, oxidative stress biomarkers, and antioxidants in aborting and nonaborting goats infected with Border disease virus. Bull Vet Inst Pulawy 57:371-374.
Campbell JR (2004) Effect of bovine viral diarrhea virus in the feedlot. Vet Clin North Am Food Anim Pract 20:39-50.
Chomczynski P, Sacchi N (2006). The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat Protoc 1:581-585.
Çam Y, Atalay Ö, Atasever A (2009). Plasma malondialdehyde level and erythrocyte catalase activity in lambs and ewes infected with sheep pox. Indian Vet J 86:525-526.
Fairbanks VF, Klee GG (1987) Biochemical aspect of haematology. In: Tiez, NW (eds), Fundamentals of Clinical Chemistry. WB Saunders, Philadelphia: pp 803-804.
Fenner FJ, Bachmann PA, Gibbs EPJ, Murphy FA, Studdert MJ, White DO (1987) Laboratory diagnosis of viral diseases. In: Fenner, FJ (eds), Veterinary Virology. Academic Press, California: pp 237-264.
García-Pérez AL, Minguijón E, Estévez L, Barandika JF, Aduriz G, Juste RA, Hurtado A (2009) Clinical and laboratorial findings in pregnant ewes and their progeny infected with Border disease virus (BDV-4 genotype). Res Vet Sci 86:345-352.
Gil L, Martínez G, González I, Tarinas A, Alvarez A, Giuliani A, Molina R, Tápanes R, Pérez J, León OS (2003) Contribution to characterization of oxidative stress in HIV/AIDS patients. Pharmacol Res 47:217-224.
Gurgoze SY, Sahin T, Sevgili M, Ozkutlu Z, Ozan ST (2003) The effects of ivermectin or doramectin treatment on some antioxidant enzymes and the level of lipid peroxidation in sheep with natural sarcoptic scap. Van Vet J 14:30-34.
Gutteridge JM (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41:1819-1828.
Halliwell B, Gutteridge JMC (1999) Free Radicals in Biology and Medicine, 3rd ed., Oxford University Press, New York: pp 246-351.
Hussin AA, Woldehiwet Z (1994) Border disease virus: a review. Vet Bulletin 64:1131-1151.
Jareño EJ, Bosch-Morell F, Fernández-Delgado R, Donat J, Romero FJ (1998) Serum malondialdehyde in HIV seropositive children. Free Radic Biol Med 24:503-506.
Kohen R, Nyska A (2002) Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 30:620-650.
Kumaraguruparan R, Balachandran C, Manohar BM, Nagini S (2005) Altered oxidant-antioxidant profile in canine mammary tumours. Vet Res Commun 29:287-296.
Luck H (1955) Catalase. In: Bergmeyer, HU (eds.), Methods in Analysis, Academy Press, London: pp 855-884.
Marnett LJ (2002) Oxy radicals, lipid peroxidation and DNA damage. Toxicol 27:219-222.
Nettleton PF, Gilray JA, Russo P, Dlissi E (1998) Border disease of sheep and goats. Vet Res 29:327-340.
Nisbet C, Yarim GF, Gumusova SO, Yazici Z (2007) Investigation of the antioxidative metabolism in sheep with peste des petits ruminants. Acta Vet Beograd 57:351-356.
Ogunro PS, Ogungbamigbe TO, Ajala MO, Egbewale BE (2005) Total antioxidant status and lipid peroxidation in HIV-1 infected patients in a rural area of south western Nigeria. Afr J Med Med Sci 34:221-225.
O’Neill RG, O’Connor, O’Reilly PJ (2004) A survey of antibodies to pestivirus in sheep in the Republic of Ireland. Ir Vet J 57:525-530.
Paglia, DE, Valentie, WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158-169.
Panda D, Patra RC, Nandi S, Swarup D (2009) Oxidative stress indices in gastroenteritis in dogs with canine parvoviral infection. Res Vet Sci 86:36-42.
Romero FJ, Bosch-Morell F, Romero MJ, Jareño EJ, Romero B, Marín N, Romá J (1998) Lipid peroxidation products and antioxidants in human disease. Environ Health Perspect 106:1229-1234.
Schweizer M, Peterhans E (1999) Oxidative stress in cells infected with bovine viral diarrhoea virus: a crucial step in the induction of apoptosis. J Gen Virol 80:1147-1155.
Sun LJ, Li SC, Zhao YH, Yu JW, Kang P, Yan BZ (2013) Silent information regulator 1 inhibition induces lipid metabolism disorders of hepatocytes and enhances hepatitis C virus replication. Hepatol Res 43:1343-1351.
Sun Y, Oberley LW, Li YAA (1988) Simple for clinical assay of superoxide dismutase. Clin Chem 34:497-500.
Suntres ZE, Omri A, Shek PN (2002) Pseudomonas aeruginosa-induced lung injury: role of oxidative stress. Microb Pathog 32:27-34.
Sweasey D, Patterson DS, Richardson C, Harkness JW, Shaw IG, Williams WW, (1979) Border disease: a sequential study of surviving lambs and an assessment of its effect on profitability. Vet Rec 104:447-450.
Treitinger A, Spada C, Verdi JC, Miranda AF, Oliveira OV, Silveira MV, Moriel P, Abdalla DS (2000) Decreased antioxidant defence in individuals infected by the human immunodeficiency virus. Eur J Clin Invest 30:454-459.
Valdazo-González B, Alvarez M, Sandvik T (2008) Prevalence of border disease virus in Spanish lambs. Vet Microbiol 30:269-278.
Valdazo-González B, Alvarez-Martínez M, Sandvik T (2007) Genetic and antigenic typing of border disease virus isolates in sheep from the Iberian Peninsula. Vet J 174:316-324.
Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ (1994) Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 136:309-323.
Webb C, Lehman T, McCord K, Avery P, Dow S (2008) Oxidative stress during acute FIV infection in cats. Vet Immunol Immunopathol 15:16-24.
Witterbourn CC, Hawkins RE, Brain M, Carrel W (1975) The estimation of red cell superoxide dismutase activity. J Lab Clin Med 55:337-341.
Wyovet (2003) Wyoming Animal Health and Disease Information Network, Wyoming State Veterinary Laboratory. BVD in Wyoming update. http://www. wyovet.uwyo.edu.
Yagi K (1998) Simple assay for the level of total lipid peroxides in serum or plasma. In: Armstrong D (eds), Free radical and Antioxidant Protocols. Humana Pres, Totowa, New Jersey: pp 101-107.
Yoshioka T, Kawada K, Shimada T (1979) Lipid peroxidation in material and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 135:372-376.