Isolation of Salmonella from the egg-laying producing plant


P. KOIDIS (Π. ΚΟΙΔΗΣ)
M. BORI (Μ. ΜΠΟΡΗ)
Résumé
In the present paper, the presence of Salmonella spp. in the egg-laying hens producing plants in Thessaloniki area was studied. In total, 2.230 eggshell samples, 588 samples surfaces from floor, walls, conveyor belts, utensils and packaging areas, 168 feed samples and 68 water samples were examined. The survey showed that the rate of contamination in the eggshells was 0.29-3.29% (mean value 1.65%). The contamination rate of the surrounding area of the cages (floor, walls, conveyor belts) and the surfaces in the packaging areas were 6.81% and 7.58% respectively. The feed was contaminated at a rate of 0-6.6% (mean value 4.2%), while all the water samples were free of Salmonella spp. Eighty-six strains of Salmonella were isolated belonging to the S. enteritidis serotype (76.8%), S. bredeney (20.29%) and S. Heidelberg (2,3%). The high prevalence of S. enteritidis, in contrast to the other serotypes, isolated from the eggshells, from the area of the egg producing plants and the feed, showed that strict preventive measures should be applied for the protection of public health and the avoidance of dispersion of Salmonella in the environment.
Article Details
  • Rubrique
  • Research Articles
Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
D'Aoust JY. Salmonella. In: Foodborne Bacterial Pathogens. Doyle MP, ed. Marcel Dekker, Inc. N.Y. and Basel, 1989,
-445
D'Aoust JY. Salmonella species. In: Food Microbiology, Fundamentals and Frontiers. Doyle MP, Beuchat LR, Montulle TJ, eds. ASM Press. Washington D.C 1997
Board RG. The microbiology of eggs. In: Egg Science and Technology. Stadelman WJ, Cotterill OJ. Eds. AVI Pubi. Connecticut, 1977,49-64
Todd EC. Foodborne and waterborne disease in Canada 1984. Annual Summary. J Food Prot 1989,53:501-503
Bean NH, Griffin PM. Foodborne outbreaks in the United States 1973-1987. Pathogens, vehicles and trends. Food. Prot 1990,53:804-817
Morris GK. Salmonella enteritidis and eggs: Assessment of risk. Dairy Food and Envin Sanit 1990,5:279-281
Tauxe RV. Salmonella: A post-modern pathogen. J Food Prot 1991,54:563-568
Altektuse S, Koehler J. A comparison of S. enteritidis phage types from egg-associated outbreaks and implicated laying flocks. Epidemiol Infect 1993,110:22-25
Centers for Disease Control. Outbreak of Salmonella enteritidis associated with homemade ice cream - Florida, 1993. Morbid Mortal Weekly Rep 1994,43:669-671
Centers for Disease Control. Update: Salmonella enteritidis infections and shell eggs - United States, 1990. Morbid Mortal Weekly Rep 1990,39:909-912
Humphrey Τ J. Contamination of egg shell and contents with Salmonella enteritidis: A review. Inter J Food Microbiol 1994,2:31-40
Humphrey TJ. Hygiene in egg production. Cost Action 97. Prevention of contamination of poultry meat, eggs and egg products. Franchini A, Mulder RWAW, eds. Pome, Italy, 23-25 October 1996,59-62
Perales I, Audicane A. Salmonella enteritidis and eggs. Lancet 1988, ii:1133-1136
Lin FY. Investigation of an outbreak of Salmonella enteritidis gastro-enteritis associated with consumption of eggs in a restaurant chain in Maryland. J Epidemiol 1988,128:839-844.
Cowden JM, Chistolm D, O'Mahony M. Salmonella enteritidis. Two outbreaks Salmonella enteritidis Phage 4 infectin associated with the consumption of fresh shell-egg products. Epidemiol Infect 1989,103:47-52
Gast R K, Beard CW. Production of Salmonella enteritidis contaminated eggs by experimentally infected hens. Avian Pis 1990,34:438-446
Madden J M. Salmonella enteritidis contamination of whole chicken eggs. Dairy Food and Envin Sanit 1990,5:268-270
Clay CE, Board RG. Growth of Salmonella enteritidis in artificially contaminated hens' shell eggs. Epidemiol Infect 1991,106:271-281
Gast RK, Beard CW. Detection and enumeration of Salmonella enteritidis in fresh and stored eggs laid by experimentally infected hens. J Food Prot 1992,55:52-156
Vugia DJ. 1993. Salmonella enteritidis outbreak in a restaurant chain: the continuing challenfes of prevention. Epidimiol. Infect. 110:49-61
Schmidt K. In: WHO Surveillance programme for control of foodborne infections and intoxication in Europe. Federal Institute for Health protection, 1995, Berlin
Mayes FG, anmd Takeballi MA. Microbial contamination of the hen's egg: A review. J Food Prot 1983,46:1092-1098
Timoney JF, Shivapresad HL, Baker RC, Rowe B, 1989. Egg transmission after infection of hens with Salmonella enteritidis phage 4. Vet Ree 1989,125:600-601
Shivaprasad HL, Timone JF, Morales S, Lucio Β, Baker RC. Pathogenesis of Salmonella enteritidis infection in laying chickens. I. Studies on egg transmission, clinical signs, faecal shedding and serologic responses. Avian Pis 1990,34:548-557
Ziprin RL. Salmonella. In: foodborne Disease Handbook, vol.1. Diseases Caused by bacteria. Hui YH, Gorham JR, Murreil KD, Cliver DO. Eds. Marcel Dekker, Inc. New York. Basel. Hong Kong. 1994,253-318
Chen L, Clarke RC, Griffiths MW. Use of luminescent strains of Salmonella enteritidis to monitor contamination and survival in eggs. J Food Prot 1996,59:915-921
Miyamoto T, Horie T, Baba E, Sasai Κ, Fukata Τ, Arakawa Α. Salmonella penetration through eggshell associated with freshness of laid eggs and refrigeration. J Food Prot 1998, 61:350-353
Wang H, Slavik MF. Bacteria penetration into eggs washed with various chemicals and stored at different temperatures and times. J Food Prot 1998,61:276-279
ISO. Microbiology - General guidance on methods for the detection of Salmonella. ISO 6579,1981
Andrews WH. A review of culture methods and their relation to rapid methods for the detection of Salmonella in foods. Food Technol 1985,77-82
Truszczynski M, Hoszowski A. Diagnosis and control of Salmonella infection in poultry. Cost Action 97 – Pathogenic mocro-organisms in poultry and eggs 1. Protection of poultry from foodborne pathogens. Nagy B, Nurmi E, Mulder RWAW, eds. Budapest, Hungary, 1995,57-59
Quinn PJ, Carter ME, Markey B, Carter GR. Antimicrobial Agents. In: Clinical Veterinary Microbiology, London Wolfe Publishing Ltd, 1994:95-102
Härtung, M. Control of Salmonella enteritidis in foods and animals 1991. Dtsch Tierarztl Wschr 1993,100:259-261
Burrow H. Isolation of Salmonella enteriditis from hen's eggs. Arch Lebensmittelhyg 1991,42:39-41
Rampling, A. Salmonella enteritidis 5 years on. Lancet 1993, 342:317-318
Παπαδάκης Ι, Μπομπότη Π, Τριχόπουλος Δ. Μόλυνση αυγών με σαλμονέλλες. 3ο Πανελλήνιο Κτηνιατρικό Συνέδριο. Κέρκυρα 1984
Mahmud ΒΑ. Διερεύνηση και μελέτη των πηγών μολύνσεως των αυγοπαραγωγικών σμηνών από σαλμονελλες. Διδακτορική διατριβή. Θεσσαλονίκη 1991
Ιορδανίδης Π, Γεωργακοποΰλου Ι. Έλεγχος της ανθεκτικότητας στελεχών σαλμονελλών των πτηνών σε αντιμικροβιακά φάρμακα. Δελτίον Ελλ. Κτην. Εταιρείας 1998, 49 (2):34-136
Goren Ε. Barcterial drug resistance is still a major problem? World Poultry 1994,10:53
Mitcell MJ, Yee AJ. Antibiotic use in animals and transfer of drug resistance to humans: Should we stop treating animals with these drugs? Dairy Food and Envin Sanit 1995, 8:484-487
Mitcell MJ, Griffiths MW, McEwen SA, McNab WB, Yee AJ. Antimicrobial drug residues in milk and meat: Causes, concerns, prevalence, regulation, tests and tests performance. J Food Prot 1998,61:742-756
Hobbs BC. Food poisoning in England and Wales. In: The microbiological safely of food, Hobs BC, Christian JHB, eds. Academic Press, London and New York 1973
Burrow H, Lo veil MA. Experimental infection of egg-laying hens with Salmonella enteritidis phage type 4. Avian Pathol 1991,20:335-348
Βογιάζας Δ. Έρευνα επί της συχνότητας μολύνσεως υπό σαλμονελλών του κυκλώματος, πτηνοτροφαί - ζώντα ορνίθια - σφάγια αυτών - πτηνοσφαγεία εις την περιοχήν Θεσσαλονίκης. Διατριβή επί διδακτορία 1977
Παπαβασιλείου Ι. Σαμαράκη-Λυμπεροποΰλου Β, Κουιμουτσοποΰλου Ι. Αναζήτηση σαλμονελλών σε τροφές ορνίθων. Δελτ. Ελλ. Μικροβ. Υγιεινολ. Εταιρείας 1965,10:161-170
Jones ET, Axtel RC, Rivers DV, Scheideier SE, Terver FR, Walker RL, Wieland MJ. A survey of salmonella contamination in modern broiler production. J Food Prot 1991,54:502-508
Dondo A, Doglione L. Pilot control plant of poultry Salmonellosis in Piedmont: A screening of pathogens. Cost Action 97 - Pathogenic micro - organisms in poultry and
eggsl. Protection of poultry from foodborne pathogens Nagy B, Nurmi E, Mulder RWAW eds. Budapest, Hungary, 1995, 119-12
Edel W. Salmonella enteritidis eradication programme in poulry breeder flocks in the Netherlands. Inter J Food Microbiol 1994,21:171-176
WHO (World Health Organization). Guidenes on prevention and control of salmonellosis VPH/83.1983,42:13-22
Articles les plus lus par le même auteur ou la même autrice