Toxoplasma gondii in sheep and goat livers: Risks for human consumption

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DOI : https://doi.org/10.12681/jhvms.20344
S. BAHRAMI
Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
M. ZAREI
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
M. GHORBANPOUR
Department of Microbiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
S. KARAMI
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Résumé

Toxoplasmosis is one of the most important zoonotic diseases worldwide and is caused by the protozoan Toxoplasma gondii. Besides vertical transmission during pregnancy, humans can become infected post-natally either by oral uptake of sporulated Toxoplasma oocysts or through ingestion of tissue cysts upon consumption of raw or undercooked meat. The aim of this study was to approximate the risk of human infection via liver consumption by estimating the seroprevalence and molecular prevalence of T. gondii in slaughtered sheep and goats in Iran. In the present study, livers from 150 sheep and 150 goats were collected at slaughter. In- house enzyme-linked immunosorbent assay was performed in T. gondii liver juice. Parasite-specific polymerase chain reaction was carried out on all samples obtained from liver tissues. Antibodies against T. gondii were detected by in-house ELISA in 32.6% sheep and 48% goat livers and 8% and 11.3% of sheep and goat livers were positive for the presence of T. gondii DNA, respectively. The results of this study provide baseline information on the presence of T. gondii in sheep and goats livers and imply an important human health and hygienic risk associated with the consumption of raw or undercooked liver from these animal species.

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Références
Bacci C, Vismarra A, Mangia C, Bonardi S, Bruini I, Genchi M, Kramer L, Brindani F (2015) Detection of Toxoplasma gondii in freerange, organic pigs in Italy using serological and molecular methods. Int J Food Microbiol 202: 54-56.
Barros M, Cabezón O, Dubey JP, Almería S, Ribas MP, Escobar LE, Ramos B, Medina-Vogel G (2018) Toxoplasma gondii infection in wild mustelids and cats across an urban-rural gradient. PLoS One 13(6): e0199085.
Basso W, Hartnack S, Pardini L, Maksimov P, Koudela B, Venturini MC, Schares G, Sidler X, Lewis FI, Deplazes P (2013) Assessment of diagnostic accuracy of a commercial ELISA for the detection of Toxoplasma gondii infection in pigs compared with IFAT, TgSAG1-ELISA and Western blot, using a Bayesian latent class approach. Int. J. Parasitol 43: 565-570.
Bastein P (2002) Molecular diagnosis of toxoplasmosis. Trans R Soc Trop Med Hyg 96: 205-215.
de A Dos Santos CB, de Carvalho AC, Ragozo AM, Soares RM, Amaku M, Yai LE, Dubey JP, Gennari SM (2005) First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil. Vet Parasitol 131: 207-211.
Dubey JP, Jones JL (2008) Toxoplasma gondii infection in humans and animals in the United States. Int J Parasitol 11: 1257-1278.
Dubey JP (2010) Toxoplasma gondii infections in chickens (Gallus domesticus): prevalence, clinical disease, diagnosis and public health significance. Zoonoses Public Health 57: 60-73.
EFSA (2007) Surveillance and monitoring of Toxoplasma in humans, food and animals, Scientific Opinion of the panel on Biological Hazards. EFSA J 583: 1-64.
Ferguson DJ, Hutchison WM, Pettersen E (1989) Tissue cyst rupture in mice chronically infected with Toxoplasma gondii. An immunocytochemical and ultrastructural study. Parasitol Res 75: 599-603.
Halos L, Thébault A, Aubert D, Thomas M, Perret C, Geers R, Alliot A, Escotte-Binet S, Ajzenberg D, Dardé ML, Durand B, Boireau P, Villena I (2010) An innovative survey underlining the significant level of contamination by Toxoplasma gondii of ovine meat consumed in France. Int J Parasitol 40: 193-200.
Hillyer GV, Soler de Galanes M, Rodriguez-Perez J, Bjorland J, Silva de Lagrava M, Ramirez Guzman S, Bryan RT (1992) Use of the Falcon assay screening test--enzyme-linked immunosorbent assay (FAST-ELISA) and the enzyme-linked immunoelectrotransfer blot (EITB) to determine the prevalence of human fascioliasis in the Bolivian Altiplano. Am J Trop Med Hyg 46: 603-609.
Jalal S, Nord CE, Lappalainen M, Evengård B (2004) Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR. Clin Microbiol Infect 10: 937-939.
Kijlstra A, Jongert E (2008) Control of the risk of human toxoplasmosis transmitted by meat. Int J Parasitol 38: 1359-1370.
Lundén A, Lind P, Engvall EO, Gustavsson K, Uggla A, Vågsholm I (2002) Serological Survey of Toxoplasma gondii in pigs slaughtered in Sweden. Scand J Infect Dis 34: 362-365.
Machala L, Kodym P, Malý M, Geleneky M, Beran O, Jilich D (2015) Toxoplasmosis in immunocompromised patients. Epidemiol Mikrobiol Imunol 64(2):59-65.
Martínez-Flores WA, Palma-García JM, Caballero-Ortega H, Del Viento-Camacho A, López-Escamilla E, Martínez-Hernández F, Vinuesa P, Correa D, Maravilla P (2017) Genotyping Toxoplasma gondii with the B1 gene in naturally infected sheep from an endemic region in the pacific coast of Mexico. Vector Borne Zoonotic Dis 17(7):495-502.
Meemken D, Tangemann AH, Meermeier D, Gundlach S, Mischok D, Greiner M, Klein G, Blaha T (2014) Establishment of serological herd profiles for zoonoses and production diseases in “pigs by meat juice multi serology”. Prev Vet Med 113: 589-598.
Mie T, Pointon AM, Hamilton DR, Kiermeier A (2008) A qualitative assessment of Toxoplasma gondii risk in ready-to-eat small goods processing. J Food Prot 71: 1442-1452.
Miao Q, Huang SY, Qin SY, Yu X, Yang Y, Yang JF, Zhu XQ, Zou FC (2015) Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP. Parasit Vectors 8: 57.
Montoya JG, Liesenfeld O (2004) Toxoplasmosis. Lancet 363: 1965-1976.
Weiss LM, Dubey JP (2009) Toxoplasmosis: A history of clinical observations. Int J Parasitol 39: 895-901.
Wingstrand A, Lind P, Haugegaard J, Henriksen SA, Bille-Hansen V, Sørensen V (1997) Clinical observations, pathology, bioassay in mice and serological response at slaughter in pigs experimentally infected with Toxoplasma gondii. Vet Parasitol 72: 129-140.
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