The survivin gene expression in neoplastic hepatocytes from chickens infected with Marek’s virus


R. NASIRI
M. GHOLAMI-AHANGARAN
P. EBADI
Résumé
P53 protein is one of the main proteins in apoptosis pathway. The role of survivin protein in inhibition of P53 in different human cancers has been proved. The expression of survivin can be a main marker in diagnosis and prognosis of different human cancer. Until now, the survivin gene expression in birds infected with Marek’s disease was not investigated. In this study, liver tissue samples of chickens infected with Marek’s disease virus (MDV) was collected. The identification of MDV was carried out with PCR and histopathologic examination. After identification of MDV, the pathogenicity of infected virus was investigated with specific primers targeted on 132 bp tandem repeat. After this, the survivin gene expression was examined in neoplastic liver samples by Real-Time PCR. The PCR amplification of tumor liver samples showed that all samples were infected with MDV. The result of histopathology and amplification of 132 bp tandem repeat in tumor samples showed that the chickens were infected to pathogenic MDV. Results showed that the survivin gene expression in neoplastic hepatocytes was significantly higher than normal hepatocytes. In conclusion, survivin gene expression can be utilized as a suitable biomarker in diagnosis of Marek’s disease in birds. It seems that viral meq oncogene in Marek’s disease can play role in induction of survivin expression in this disease that it is necessary to be proved.
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Références
Altieri DC, Marchisio C (1999) Survivin apoptosis: an interloper between cell death and cell proliferation in cancer. Lab Invest 79: 1327–1333.
Altieri DC (2015) Survivin-the inconvenient IAP. Semin Cell Dev Biol 39: 91-96.
Ambrosini G, Adida C, Altieri DC (1997) A novel anti-apoptosis gene survivin expressed in cancer and lymphoma. Nat Med 3: 917–921.
Johnson ME, Howerth EW (2004) Survivin: A Bifunctional Inhibitor of Apoptosis Protein Vet Pathol 41: 599-607.
Bancroft JD, Stevens A (1996) Theory and practice of histopathological techniques. Churchill livingstone: London: 100-110.
Becker Y, Asher Y, Tabor E, Davidson I, Malkinson M, Weisman Y (1992) Polymerase chain reaction for differentiation between pathogenic and nonpathogenic serotype 1 Marek’s disease viruses (MDV) and vaccine viruses of MDV-serotypes 2 and 3 J Virol Methods 40: 307-322.
Berendse M, Grounds MD, Lioyd CM (2003) Myoblast structure affects subsequent skeletal myotube morphology and sarcomere assembly Exp Cell Res 291: 435-450.
Biggs PM (2001) The history and biology of Marek disease virus Curr Top Microbiol Immunol 255:1-24.
Borbely AA, Murvai M, Konya J, Beck Z, Gergely L, Li F (2006) Effect of human papillomavirus type 16 oncoproteins on survivin gene expression J Gen Virol 87: 287–294.
Calnek BW (2001) Pathogenesis of Marek disease virus infection Curr Top Microbiol Immunol 255:25-55.
Chen JC, Goldhamer DJ (2003) Skeletal muscle stem cells Reported boil Endocrinol 1: 101-107.
Fan Y, Liu Z, Zhao S, Lou F, Nilsson S, Ekman P (2006) Nucleostemin mRNA is expressed in both normal and malignant renal tissues Brit J Cancer 94: 1658-62.
Grossman D, McNiff JM, Li F, Altieri DC (1999) Expression of the apoptosis inhibitor survivin in nonmelanoma skin cancer and gene targeting in a keratinocyte cell line Lab Invest 79: 1121–1126.
Hino R, Uozaki H, Inoue Y, Shintani Y, Ushiku T, Sakatani T (2008) Survival advantage of EBV-associated gastric carcinoma: surviving up-regulation by viral latent membrane protein 2A. Cancer Res. 68:1427–1435.
Ito T, Shiraki K, Sugimoto K, Yamanaka T, Fujikawa K, Ito M (2000) Survivin promotes cell proliferation in human hepatocellular carcinoma. Hepatol 31: 1080-1085.
Kato J, Kuwabara Y, Mitani M, Shinoda N, Sato A, Toyama T( 2001) Expression of survivin in esophageal cancer: correlation with the prognosis and response to chemotherapy. Int J Cancer 95: 92-95.
Kawakami H, Tomita M, Matsuda T, Ohta T, Tanaka Y, Fujii M, Hatano M, Tokuhisa T, Mori N (2005) Transcriptional activation of surviving through the NF-kappaB pathway by human T-cell leukemia virus type I tax. Int J Cancer 115: 967-974.
Kozdrun W, Samorek-Salamonowicz E, Czekay H (2001) PCR for the differentiation of Marek’s disease virus strain. Bull Vet Inst Pulway 45: 5-10.
Lei Y, Geng Z, Guo-Jun W, He W, Jian-Lin Y (2010) Prognostic significance of survivin expression in renal cell cancer and its correlation with radioresistance. Mol Cell Biochem 344: 23-31.
Li YP, Bang DD, Handberg KJ, Jorgensen PH, Zhang MF (2005) Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus. Vet Mic 110: 155-165.
Marek J (1907) Multiple Nervenentzuendung (Polynephritis) bei ituehnern Dtsch Tiemztl Wochenschr 15:417-421.
Marusawa H, Matsuzawa S, Welsh K, Zou H, Armstrong R, Tamm I (2003) HBXIP functions as a cofactor of survivin in apoptosis suppression. EMBO J 22: 2729-2740.
Nachmias B, Ashhab Y, Ben-Yehud D (2004) The inhibitor of apoptosis protein family (IAPs): an emerging therapeutic target in cancer. Semin Cancer Biol 14: 231-243.
Nair V, Kung HJ (2004) Marek’s disease virus oncogenicity: molecular mechanisms. In: Marek’s Disease. Academic Press, New York: 32-48.
Olie RA, Simoes-Wust AP, Baumann B, Leech SH, Fabbro D, Stahel RA (2000) A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy. Cancer Res 60: 2805-2809.
Saitoh Y, Yaginuma Y, Ishikawa M (1999) Analysis of Bcl-2 Bax and surviving genes in uterine cancer. Int J Oncol 15: 137–141.
Schat KA, Nair V (2013) Marek’s disease In: Disease of Poultry, 13th ed, Wiley-Blackwell, Massachusetts: 515-565.
Shahzad MK, Majeed KA, Younus M (2007) Marek’s Disease: A Mini-Review. Int J Agro Vet Med Sci 1: 4-8.
Shaikh Z, Niranjan KC (2015) Tumour biology: p53 gene mechanisms. JClin Cell Immunol 6: 12-18.
Swana HS, Grossman D, Anthony JN, Weiss RM, Altieri DC (1999) Tumor content of the antiapoptosis molecule surviving and recurrence of bladder cancer. N Eng J Med 341: 452–453.
Tamm I, Kornblau SM, Segall H (2000) Expression and prognostic significance of IAP-family genes in human cancers and myeloid leukemias. Clin Cancer Res 6: 1796- 803.
Tulman ER, Afonso CL, Lu Z, Zsak L, Rock DL, Kutish GF (2000) The genome of a very virulent Marek disease virus J Virol 74:7980-7988.
Verdecia MA, Huang H, Dutil E, Kaiser DA, Hunter T, Noel JP (2000) Structure of the human anti-apoptotic protein survivin reveals a dimeric arrangement. Nat Struct Biol 7: 602-608.
Yoshida H, Ishiko O, Sumi T, Matsumoto Y, Ogita S (2001) Survivin bcl-2 and matrix metalloproteinase-2 enhance progression of clear cell- and serous-type ovarian carcinomas. Int J Oncol 19: 537-542.
Zhao Y, Kurian D, Xu H, Petherbridge L, Smith LP, Hunt L (2009) Interaction of Marek’s disease virus oncoprotein Meq with heat-shock protein 70 in lymphoid tumour cells. J Gen Virol 90: 2201–2208.
Zhu GS, Ojima T, Hironaka T, Ihara T, Mizukoshi N, Kato A (1992) Differentiation of oncogenic and non-oncogenic strains of MDV type 1 by PCR DNA amplification. Avian Dis 36: 637-645.