Studies on Pseudomonas aeruginosa Infection in Hatcheries and Chicken

PDF (English)
DOI : https://doi.org/10.12681/jhvms.22937
R. D. ERAKY
Bacteriology Department, Animal Health Research Institute (Damietta branch), Egypt
W. A. ABD EL-GHANY
Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University, Egypt
K. M. SOLIMAN
Pathology Department, Animal Health Research Institute, Dokki, Egypt
Résumé

The aim of this work was to spot light on the presence of Pseudomonas aeruginosa (P. aeruginosa) strains in hatcheries and dead in shell embryos. A total of 406 samples representing 200 and 206 swabs from hatcheries environment and yolk sacs of dead in shell embryos were collected from Damietta governorate, Egypt. P. aeruginosa was isolated and identified. Some virulent genes (toxA, psIA and fliC) of P. aeruginosa were detected using polymerase chain reaction (PCR). The antimicrobial susceptibility of P. aeruginosa was tested in vitro. Day and 11 days old broiler chicks were challenged with P. aeruginosa to determine the pathogenicity of the isolated strains. The results showed that P. aeruginosa was recovered from 16 (8%) out of 200 hatcheries and from 17 (8.25%) out of 206 chicken embryos samples. Isolated strains of P. aeruginosa showed presence of toxA, psIA and fliC virulent genes. P. aeruginosa strains were sensitive (100%) to ciprofloxacin, levofloxacin and gentamycin but resistant (100%) to amoxycillin/clavulanic acid, doxycycline and erythromycin. The pathogenicity test of day and 11 days old chicks revealed that P. aeruginosa was highly pathogenic induced mortality rates of 72 and 40%, respectively. Septicaemia of internal organs, unabsorbed yolk sacs, pneumonia, greenish exudates in the abdominal cavity, liver necrosis and enteritis were the predominant lesions. Histopathological changes supported the previous lesions. In conclusion, P. aeruginosa is of great importance pathogen of embryos and newly hatched chicks based on presence of virulent genes as well as in vivo pathogenicity study; respectively.

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Al-Ahmadi JG, Roodsari ZR (2016) Fast and specific detection of Pseudomonas aeruginosa from other Pseudomonas species by PCR. Annals of Burns and Fire Disasters, XXIX 4: 264-267.
Anuj SN, Whiley DM, Kidd TJ, Bell SC (2009) Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecfX and the gyrB genes. Diagn Microbiol Infec Dis 63: 127-131.
Bancroft JD, Gamble M (2002) Theory and practice of histological technique. 6th Ed., Churchill, Livingston, New York, London, San Francisco, Tokyo.
Barnes HJ (2003) Miscellaneous Bacterial Diseases. In: Diseases of Poultry. 11th ed. Iowa State University Press, Ames, pp. 852-854.
Clinical Laboratory and Standards Institute (CLSI) (2015) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard - tenth edition. CLSI document M07-A10 Villanova, PA, USA: CLSI, 2015.
Dong D, Zou D, Liu H, Yang Z, Huang S, Liu N, He X, Liu W, Huang L (2015) Rapid detection of Pseudomonas aeruginosa targeting the toxA gene in intensive care unit patients from Beijing, China. Frontier Microbiol 6: 1100.
El-Gohary Amany H, El-Shehedi Mona A, Safwat E (2012) Characterization of Pseudomonas aeruginosa isolated from ration and its viability in the environment. 1st Conference of Animal Health Institute Association, Cairo, Egypt, December, pp. 729-737.
Elsayed MSA, Ammar AM, Al-Shehri ZS, Abd-El Rahman H, Abd-El Rahman NA (2016) Virulence repertoire of Pseudomonas aeruginosa from some poultry farms with detection of resistance to various antimicrobials and plant extracts. Cell Molc Biol 62: 124-128.
Ertugrul BM, Oryasin E, Lipsky BA, Willke A, Bozdogan B (2018) Virulence genes fliC, toxA and phzS are common among Pseudomonas aeruginosa isolates from diabetic foot infections. Infect Dis 50: 273-279.
Fadhil L, Al-Marzoqi AH, Zahraa MA, Shalan AA (2016) Molecular and phenotypic study of virulence genes in a pathogenic strain of Pseudomonas aeruginosa isolated from various clinical origins by PCR: Profiles of genes and toxins. Res J Pharmaceut Biol Chem Sci 7: 590-598.
Farghaly EM, Roshdy H, Bakheet AA, Abd El-Hafez SA, Badr H (2017) Advanced studies on Pseudomonas aeruginosa infection in chicken. Anim Health Res J 5: 207-217.
Fekadu K (2010) Pseudomonas infection in chickens. J Vet Med Anim Health 2: 55-58.
Ferro PJ, Swaggerty CL, Kaiser P, Pevzner IY, Kogut MH (2004) Heterophils isolated from chickens resistant to extra intestinal Salmonella enteritidis infection express higher levels of pro-inflammatory cytokine mRNA following infection than heterophils from susceptible chickens. Epidemiol Infec 132: 1029-1037.
Forbes B, Sahm D, Weissfeld A (2007) Bailey and Scott’s Diagnostic Microbiology. Elsevier, Science Publishing Company, New York, 12th edition, pp. 322-325.
Ghadaksaz A, Fooladi AAA, Hosseini HH, Amin M (2015) The prevalence of some Pseudomonas virulence genes related to biofilm formation and alginate production among clinical isolates. J App Biomed 13: 61-68.
Homma JY (1980) Designation of thirteen “O” group antigen of Pseudomonas aeruginosa attachment for the tentative proposal in 1976. Jap Experim Med 52: 317-321.
Hayford O (2017) Isolation and characterisation of multi-drug resistant Pseudomonas aeruginosa from clinical, environmental and poultry litter sources in Ashanti region of Ghana. Mv.Sc, Thesis, Kwame Nkrumah University of Science and Technology, Kumasi- Ghana.
Joh SJ, Kim MC, Kwon YK, Kim JH (2005) Occurrence of Pseudomonas aeruginosa infection in the broilers in Korea. Korean J Vet Res 45: 71-74.
Katja M, Vishal K, Torben K, Namir S, Nadine H, Vikas D et al. (2015) Virus-induced type I interferon deteriorates control of systemic Pseudomonas aeruginosa Infection. Cell Physiol Biochemi 36: 2379-2392.
Lister PD, Wolter DJ, Hanson ND (2009) Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 22:582-610.
Matar GM, Ramlawi F, Hijazi N, Khneisser I, Abdelnoor AM (2002) Transcription levels of Pseudomonas aeruginosa exotoxin A gene and severity of symptoms in patients with otitis externa. Curr Microbiol 45: 350-354.
Michalska M, Wolf P (2015) Pseudomonas exotoxin A: optimized by evolution for effective killing. Frontier Microbiol 6: 963.
Mohamed HA (2004) Some studies on pseudomonas species in chicken embryos and broilers in Assiut governorate. Assuit Univ Bull Environ Res 7: 23-31.
Nedeljković NS, Tiodorović B, Kocić B, Ćirić V, Milojković M, Waisi H (2015) Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs. Vojnosanitetski Pregled, 72: 996-1003.
Quinn PJ, Markey BK, Carter ME, Donnelly WJC, Leonard FC (2002) Veterinary Microbiology and Microbial Disease. First published A Blackwell Science Company.
Rasamiravaka T, Labtani, Q, Duez P, ElJaziri M (2015) The Formation of biofilms by Pseudomonas aeruginosa: A review of the natural and synthetic compounds interfering with control mechanisms. BioMed Res Inter 1-17.
Rejman J, Di Gioia S, Bragonzi A, Conese M (2007) Pseudomonas aeruginosa infection destroys the barrier function of lung epithelium and enhances polyplex-mediated transfection. Human Gene Therapy 18:642-652.
Satish S, Priti M (2015) Pseudomonas aeruginosa infection in broiler chicks in Jabalpur. Inter J Extensive Res 6: 37-39.
Supartika IK, Toussaint MJ, Gruys, E (2006) Avian hepatic granuloma. A review. Vet Q 28: 82-89.
Timurkaan N, Yilmaz F, Kilic A, Ozbey G (2008) Pathological and microbiological investigations on lung lesions of slaughtered broilers in the slaughterhouse. J Anim Vet Adv 7: 1358-1363.
Van Delden CV (2004) Virulence factors in Pseudomonas aeruginosa. In: Pseudomonas: virulence and gene regulation. Kluwer Academic/Plenum Publishers, New York, pp. 23-45.
Walker SE, Sander JE, Cline JL, Helton JS (2002) Characterization of Pseudomonas aeruginosa isolates associated with mortality in broiler chicks. Avian Dis 46: 1045-1050.
Wei Q, Ma LZ (2013) Biofilm matrix and its regulation in Pseudomonas aeruginosa. Inter J Mol Sci 14 : 20983-21005.