Effects of L-carnitine and cryodevices on the vitrification and developmental competence of invitro fertilized buffalo oocytes

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Publiée : Ιουλ 9, 2022
DOI : https://doi.org/10.12681/jhvms.24581
Mohammed A Elmetwally
Associate Professor of Animal Reproduction, Faculty of Veterinary Medicine, Mansoura University, Egypt
https://orcid.org/0000-0001-7486-7756
Amira M Mostagir
Department of Theriogenology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt; Center for Reproductive Biotechnology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt;
Fuller W Bazer
Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA 77843, USA
Abdelmonem Montaser
Department of Theriogenology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt; Center for Reproductive Biotechnology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt
Magdy Badr
4Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram, Giza, Egypt
Wael Eldomany
1Department of Theriogenology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt; 2 Center for Reproductive Biotechnology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt
Ashraf Eldesoky
1Department of Theriogenology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt; 2 Center for Reproductive Biotechnology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt;
Mona M Elghareeb
Department of Physiology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt
Daniela Jaimes
Faculty of Agricultural Sciences, University of Applied and Environmental Sciences U.D.C.A, Bogota, Colombia
Yaser Y Lenis
Research group OHVRI, School of Veterinary Medicine, Faculty of Agrarian Science, Universidad de Antioquia, Calle 70, No. 52-21, Medellín, Colombia
Samy M Zaabel
1Department of Theriogenology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt; 2 Center for Reproductive Biotechnology, Veterinary Medicine Faculty, Mansoura University, Mansoura 36615, Egypt;
Résumé

In the current, study the effect of the addition of L-carnitine (LC) in in vitro maturation (IVM) medium for buffalo oocytes and different cryo-devices on developmental competence. They were matured in IVM medium supplemented with 0.0, 0.3, 0.6 and 1.2 mg/mL of LC and vitrified by using either straw (ST), open pulled-straw (OPS), solid surface vitrification (SSV). The effects of LC during invitro fertilization and invitro culture on the developmental potential were examined. ST showed a higher recovery rate when using 0.6 mg/ml; viability rate (VR) when using 0.3mg/ml and lower zona pellucida (ZP) and cytoplasmic abnormalities when using 1.2 mg/ml LC (P<0.05). OPS showed a higher recovery and viability rates when using 0.3 mg (P<0.05). SSD showed a higher recovery and VR when using 0.6 mg/ml LC (P<0.05). Maturation and fertilization rates were increased when using 0.3 mg LC and OPS (P<0.05). Cleavage was increased in 0.3 (OPS) and 0.6 mg LC (SSD). The blastocyst rate was increased in 0.3 (OPS) and 0.6 mg/ml (SSD) (P< 0.05). In conclusion, 0.3 mg/ml LC and OPS gives a higher recovery and viability rates but 0.6 mg/ml LC and of both SSD and ST gives a higher recovery and viability rates.

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Biographie de l'auteur
Mohammed A Elmetwally, Associate Professor of Animal Reproduction, Faculty of Veterinary Medicine, Mansoura University, Egypt
Theriogenology Department
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