Staphylococci and zoonotic potential : oral carriage and antibiotic susceptibility in stray dogs and cats in Algeria

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Publiée : Apr 29, 2022
DOI : https://doi.org/10.12681/jhvms.25239
Kahina Razali
Rachid Kaidi
Filippo Giarratana
Khatima Ait-Oudhia
Higher National Veterinary School, PB 161 Rue Issad Abbes, Oued Smar, Algiers, Algeria
https://orcid.org/0000-0002-2514-5615
Résumé

This study aimed at identifying species of staphylococci isolated from the oral cavity of dogs and cats, in Algeria, and to determine their antibiotic susceptibility.

Oral swabs were collected from 70 healthy animals (35dogs and 35cats) and were grown in Mannitol Salt Agar medium.Isolates were identified using API staph commercial kits and then confirmed with MALDI-TOF MS associated with SARAMIS software.Coagulase-positive staphylococci (CoPS) were tested for antibiotic susceptibility by disk diffusion method.

On the 70 sampled animals, 59 were carriers of staphylococci in the oral cavity and more than one species was detected in 11 of them. Seventy (70) staphyloccoccal isolates were obtained belonging to 10 defferent species. Coagulase-negative staphylococci (CoNS) were most prevalent (81.42% ),of which the dominant species was S. xylosus (40%).Other coagulase-negative species,such as S, simulans (14.28%), S ,sciuri (11.42%) , S. saprophyticus (10%),S. capitis (2.85%),S, cohnii subsp cohnii (1.42%), and S, epidermidis (1.42%) were also isolated.The remaining n.13 CoPS included : S. intermedius (2.85%),S .pseudintermedius (8.57%) and S. intermedius group (SIG) (4.28%). No strain of S. aureus was found.

Results of antimicrobial resistance showed that 61.53% of CoPS isolates were resistant to at least two drugs.The highest rate of resistance was observed against penicillin,ampicillin and tetracycline (53.84% for each drug),while amoxicillin-clavulanate was active on most isolates.

In Algeria,Stray dogs and cats are carriers of many staphylococci species in the oral cavity, including multidrug resistant CoPS, which could be transmitted to humans through bites.

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Biographie de l'auteur
Khatima Ait-Oudhia, Higher National Veterinary School, PB 161 Rue Issad Abbes, Oued Smar, Algiers, Algeria
Veterinary Doctor, Master in Medical Microbiology, PhD in Infectious and Parasitic Diseases at the Veterinary School of Algiers and PhD in Clinical Biology of the Faculty of Medicine of Montpellier-France. Professor of Infectious and Parasitic Diseases at the National Veterinary School of Algiers.Thematic of interest: Parasitic diseases (protozoa), abortive diseases (bacterial, viral, parasitic), vector diseases and Dromedary Pathologies.
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