Characterization of Antimicrobial Resistance Genes of Pasteurella multocida Isolated from Diseased Chickens in Egypt

PDF (English)
Publiée : Ιουλ 10, 2022
DOI : https://doi.org/10.12681/jhvms.26851
ET Bedier
Department of Bacteriology, Mycology and ImmunologyFaculty of Veterinary MedicineKafrelsheikh University
https://orcid.org/0000-0002-0034-5071
SR Labib
Bacteriology Department, Animal Health Research Institute, Dokki, Cairo, Egypt
AM Ahmed
Department of Bacteriology, Mycology and ImmunologyFaculty of Veterinary MedicineKafrelsheikh UniversityKafr EL-Sheikh 33516, Egypt
Résumé

 In this study, the prevalence of Pasteurella multocida in diseased chickens, capsular genotyping, antimicrobial resistance patterns and some resistance genes were determined. Lungs, liver and spleen samples were collected from 250 diseased chickens from layers and broiler flocks from El-Gharbia and Kafr El-Sheikh governorates in Egypt for isolation of P. multocida in the period from June 2018 to December 2019. Confirmatory identification was done by using PCR for capsular type A antigen. P. multocida was isolated from 3.6 % of the diseased chicken. Six isolates of P. multocida that examined for detection of capsular type A showed positive results. Antimicrobial resistance patterns were evaluated for all isolates against twenty antimicrobial agents and the results showed 100% resistance totrimethoprim/sulfamethazole, oxacillin and nitrofurantion. Also, strains expressed highly resistant to penicillin, chloramphenicol, rifampicin and ampicillin \sulbactam, while they were sensitive to norfloxacin, clindamycin, cephalexin and cefotaxime. The antimicrobial resistance genes were detected by using PCR and the results showed  that all isolates harbored  β-lactam-resistantgene BlaROB-1 (100%), followed by sulfonamide resistant gene  sul1 (50%), tetracycline-resistant gene tetH (33.3%) and trimethoprim-resistant dihydrofolate reductase dfrA (16.6%).

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Bibliographies de l'auteur
SR Labib, Bacteriology Department, Animal Health Research Institute, Dokki, Cairo, Egypt

Bacteriology Department, Animal Health Research Institute, professor

AM Ahmed, Department of Bacteriology, Mycology and ImmunologyFaculty of Veterinary MedicineKafrelsheikh UniversityKafr EL-Sheikh 33516, Egypt

Department of Bacteriology, Mycology and Immunology

Faculty of Veterinary Medicine, professor

 

 

Références
Akhtar M (2013) Isolation, identification and characterization of Pasteurella multocida from chicken and development of oil based vaccine. MS thesis, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh.
Al-Assil B, Mahfoud M and Hamzeh AR (2013) First report on class 1 integrons and Trimethoprim-resistance genes from dfrA group in uropathogenic E. coli (UPEC) from the Aleppo area in Syria. Mobile Genet. Elem 3: e25204.‏
Angrick E, Morishita T and Harr B. (2001) Prevalence and characteristics of Pasteurella multocida in commercial turkeys. Avian Dis 182-190.‏
Aravinth A, Prakash S and Hariharan P (2016) Incidence of Fowl Cholera with Anomalous Lesions in Laying Hens: A Case Study. Brit J Poult Sci 5: 09.
Babetsa M, Vassilios S, Vougidou C, Zdragas, A, Sivropoulou A, Psaroulaki A and Ekateriniadou L (2012) Tetracycline resistance genes in Pasteurella multocida isolates from bovine, ovine, caprine and swine pneumonic lungs originated from different Greek prefectures. Afr J Microbiol Res 6: 3917-3923.
Balakrishnan G and Roy P (2012) Isolation, identification and antibiogram of Pasteurella multocida isolates of avian origin. J Vet Anim Sci 8: 199-202.
Christensen H and Bisgaard M (2003). The genus Pasteurella. In: Dworkin, M and Lyons, C (Eds.), The prokaryotes: an evolving electronic resource for the microbiological community. (Ver.3.13), Springer-Verlag, New York. PP: 1062-1090.
Christensen, H and Bisgaard, M (2010). Molecular classification and its impact on diagnostics and understanding the phylogeny and epidemiology of selected members of Pasteurellaceae of veterinary importance. Berl Munch Tierarztl Wochenschr 123: 20–30.
CLSI (2016) Performance Standards for Antimicrobial Susceptibility Testing. 26th Edn., CLSI document M100-S26. Wayne, PA, USA.
Dashe YD, Raji MA, Abdu PA, Oladele BS and Sugun MY (2013) Multidrug resistant Pasteurella multocida strains isolated from chickens with cases of fowl cholera in Jos Nigeria Int J Poult.Sci 12: 596.‏
Davies RL, MacCorquodale R and Caffrey B (2003) Diversity of avian Pasteurella multocida strains based on capsular PCR typing and variation of the OmpA and OmpH outer membrane proteins. Vet Microbiol 91: 169-182.‏
Dayao DAE, Gibson JS, Blackall, PJ and Turni C (2016) Antimicrobial resistance genes in Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida isolated from Australian pigs. Aust Vet J 94: 227-231.‏
Elalamy RA, Tartor YH, Ammar AM, Eldesouky IE and Esawy AEI (2020) Molecular Characterization of Extensively Drug-Resistant Pasteurella multocida Isolated from Apparently Healthy and Diseased Chickens in Egypt. Pak Vet J 40:3.‏
Escande FG, Gerbaud JL, Martel and Courvalin P (1991) Resistance to trimethoprim and 2,4-diamino-6,7 diisopropyl-pteridine (O/129) in Pasteurella haemolytica. Vet Microbiol 26:107–114.
Furian TQ, Borges KA, Laviniki V, Rocha SLDS, Almeida CND, Nascimento VPD and Moraes HLDS (2016) Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine. Braz J Microbiol 47: 210-216.
Glisson JR, Hofacre CL and Christensen JP (2003) Fowl cholera. In: Diseases of poultry. 11th Ed, Iowa state press, USA: PP 658-676.
Glisson JR, Hofacre CL and Christensen JP (2008) Fowl cholera In: Diseases of Poultry. Blackwell Publishing, Ames Iowa, USA: PP 739-758.
Glisson JR, Sandhu TS and Hofacre CL (2008). Pasteurellosis, avibacteriosis; gallibacteriosis, riemerellosis and pseudotuberculosis In: A laboratory manual for the isolation, identification and characterization of avian pathogens. Georgia. Am Assoc Avian Patholog: PP 12-18.
Grape M, Motakefi A, Pavuluri S and Kahlmeter G (2007). Standard and real-time multiplex PCR methods for detection of trimethoprim resistance dfr genes in large collections of bacteria. Clin Microbiol Infect 13: 1112–1118.
Hay SI, Rao PC; Dolecek C, Day NP, Stergachis, A, Lopez AD and Murray CJ (2018). Measuring and mapping the global burden of antimicrobial resistance. BMC Med 16: 1-3.‏
Ibekwe AM, Murinda SE and Graves AK (2011) Genetic Diversity and Antimicrobial Resistance of Escherichia coli from Human and Animal Sources Uncovers Multiple Resistances from Human Sources. PLOS ONE 6: e20819.
kamruzzaman M, Islam M, Hossain MM, Hassan MK, Kabir MHB, Sabrin MS and Khan MSR (2016). Isolation, Characterization and Antibiogram Study of Pasteurella multocida Isolated from Ducks of Kishoreganj District, Bangladesh. IJAR 1: 69-76.
Kehrenberg C and Schwarz S (2000). Identification of a truncated, but functionally active tet(H) tetracycline resistance gene in Pasteurella aerogenes and Pasteurella multocida. FEMS Microbiol Lett 188: 191-195.
Kehrenberg C, Salmon SA, Watts JL and Schwarz S (2001) Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet (H) plasmid pMHT1. J. Antimicrob. Chemother 48: 631-640.‏
Khamesipour, F; Momtaz, H and Azhdary Mamoreh, M (2014) Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran. Front Microbiol., 5: 536.
Klima CL, Alexander TW, Read RR, Gow SP, Booker CW, Hannon S and Selinger, LB (2011) Genetic characterization and antimicrobial susceptibility of Mannheimia haemolytica isolated from the nasopharynx of feedlot cattle. Vet Microbiol 149: 390-398.‏
Klima CL, Alexander TW, Hendrick S and McAllister TA (2014) Characterization of Mannheimia haemolytica isolated from feedlot cattle that were healthy or treated for bovine respiratory disease. Can J Vet Res 78: 38-45.‏
Kwage JKP, Ekundayo SO, Chuku A, Yusuf AF, Mwankon ES, Boss SS and Muhammad M (2013). Phenotypic and genotypic characterization of Pasteurella multocida isolated from dead poultry in Jos, Plateau State. Niger. Vet J 34: 2.‏
Muhairwa AP, Mtambo MMA, Christensen JP and Bisgaard M (2001). Occurrence of Pasteurella multocida and related species in village free ranging chickens and their animal contacts in Tanzania. Vet Microbiol 78: 139-153.‏
Oh YH, Moon DC, Lee YJ, Hyun BH, Lim SK (2018). Antimicrobial resistance of Pasteurella multocida strains isolated from pigs between 2010 and 2016. Vet Rec Open., 5:e000293. doi:10.1136/vetreco-2018-000293.
Oh YH, Moon DC, Lee YJ, Hyun BH and Lim SK (2019). Genetic and phenotypic characterization of tetracycline-resistant Pasteurella multocida isolated from pigs. Vet Microbiol 233: 159-163.‏
OIE (2012) HAEMORRHAGIC SEPTICAEMIA (W.W.W Document). Available at www.oie.int/fileadmin/Home/fr/Healthstandards/tahm/2.04.12_HEMORRHAGIC_SEPTICEMIA.pdf .( The World Assembly of delegates of the OIE, May( 2012 .
OIE list of antimicrobial agents of veterinary importance (2014) Available at www.oie.int/fileadmin/Home/eng/Our_scientific_expertise/docs/pdf/OIE_list_antimicrobials.pdf. (accessed 22 July 2014).
Olonitola OS, Fahrenfeld N and Pruden A (2015) Antibiotic resistance profiles among mesophilic aerobic bacteria in Nigerian chicken litter and associated antibiotic resistance genes. Poult Sci 94: 867-874.‏
Panna SN, Nazir KNH, Rahman MB, Ahamed S, Saroare MG, Chakma S and Majumder UH (2015) Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens and their histopathological evaluation in artificially infected chickens in Bangladesh. J Adv Vet Anim Res 2: 338-345.‏
Projan SJ (2003) Why is big Pharma getting out of antibacterial drug discovery? Curr Opin Microbiol 6: 427-430.‏
Quinn PJ, Carter ME, Markey BK and Carter GR (1994) Veterinary Clinical Microbiology. Wolfe Publication., London, U.K. PP: 254-259.
Rahman MA, Samad MA, Rahman MB and Kabir SML (2004) In vitro antibiotic sensitivity and therapeutic efficacy of experimental salmonellosis, colibacillosis and pasteurellosis in broiler chickens. Bangladesh Journal of Veterinary Medicine 2: 99-102.
‏ Rajeev R, Panda SK, Acharya AP, Singh AP and Gupta MK (2011) Molecular diagnosis of haemorrhagic septicaemia-A review. Vet. World 4: 189.‏
Raji MA, Ahmed JS, Saidu L and Ameh JA (2010) Retrospect studies on the prevalence of fowl cholera in Zaria-Kaduda state Nigeria. SJVS 8:1-2.‏
San Millan A, Escudero JA, Gutierrez B, Hidalgo L; Garcia N, Liagostera, M and Gonzalez-Zorn, B (2009) Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids. Antimicrob Agents Chemother 53: 3399-3404.‏
Sarangi LN and Panda HK (2011) Antibiotic Sensitivity of Avian Isolates of Pasteurella multocida. Indian Vet J 88: 85-86.‏
Shivachandra SB, Kumar AA, Gautam R, Singh VP, Saxena MK and Srivastava SK (2006) Identification of avian strains of Pasteurella multocida in India by conventional and PCR assays. Vet J 172: 561-564.‏
Tang X, Zhao Z, Hu J Wu B, Cai X, He Q and Chen H (2009) Isolation, antimicrobial resistance and virulence genes of Pasteurella multocida strains from swine in China. J Clin Microbiol 47: 951-958
Thulasi GP, Indu K, Rajagopa R, Mini M, Krishnan GN, Koshy J and Siju J (2013) Isolation and Characterization of Pasteurella multocida from Poultry and Deer. proc. Natl Acad Sci 83: 621–625.
Victor AA, Mathew BA, Olubukunola OA, Ayo AO and Samuel AO (2016) Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis. Liver 8: 5.‏
Zahoor MA and Siddique, M (2006) Characteristics of Pasteurella multocida recovered from avian sources. Pak Vet J 26: 41.‏