A Comprehensive in silico Analysis of mt-CO1 gene of Fasciola hepatica

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Publiée : Ιουλ 10, 2022
DOI : https://doi.org/10.12681/jhvms.26881
Figen Celik
Firat University
https://orcid.org/0000-0002-2188-0196
Sami Simsek
Firat University
https://orcid.org/0000-0002-3567-326X
Harun Kaya Kesik
Bingöl University
https://orcid.org/0000-0002-8480-8597
Seyma Gunyakti Kilinc
Bingöl University
https://orcid.org/0000-0001-8454-1901
Résumé

This study was conducted for the aim of comprehensive evaluation of the genetic diversity and phylogenetic relations of F. hepatica among various hosts. In this study, published sequences of mt-CO1 gene fragments belonging to final hosts of F. hepatica were used to create the dataset. First of all, 478 sequences were obtained with PubMed search. Then, some shorter sequences were removed after alignment, and 319 sequences which included cattle (n=242), sheep (n=46), goat (n=5), donkey (n=8), bison (n=7), water buffalo (n=7), human (n=2) and camel (n=2) sequences were analysed in the MEGA X program. The existence of 72 haplotype groups was detected. The most polymorphic sites (n=42) containing 31% (13/42) parsimony informative sites were detected in Iranian isolates. The mt-CO1 network consisted of 35 haplotypes, 80% out of which were geographically unique. However, a main haplotype consisting of 39.2% of the total isolates was formed. The bison isolates of F. hepatica showed the highest haplotype diversity within the final host isolates, followed by the sheep, water buffalo, cattle, goat and donkey isolates. The genetic diversity of F. hepatica in different hosts and countries revealed the possibility of new strains appearing in the future.

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Biographie de l'auteur
Sami Simsek, Firat University

Department of Parasitology, Faculty of Veterinary Medicine,

University of Firat, 23119, Elazig-Turkey.

Références
Ai L, Weng Y, Elsheikha H, Zhao G, Alasaad S, Chen J, Li J, Li H, Wang C, Chen M (2011) Genetic diversity and relatedness of Fasciola spp. isolates from different hosts and geographic regions revealed by analysis of mitochondrial DNA sequences. Veterinary Parasitology 181: 329-334.
Bandelt HJ, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution 16: 37-48.
Blair D, Campos A, Cummings M, Laclette J (1996) Evolutionary biology of parasitic platyhelminths: the role of molecular phylogenetics. Parasitology Today 12: 66-71.
Dosay-Akbulut M, Trudgett A, Stanhope M (2005) Understanding genetic diversity of the liver fluke Fasciola hepatica. Zeitschrift für Naturforschung C 60: 774-778.
Fletcher H, Hoey E, Orr N, Trudgett A, Fairweather I, Robinson M (2004) The occurrence and significance of triploidy in the liver fluke, Fasciola hepatica. Parasitology 128: 69-72.
Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147: 915-925.
Ichikawa-Seki M, Shiroma T, Kariya T, Nakao R, Ohari Y, Hayashi K, Fukumoto S (2017) Molecular characterization of Fasciola flukes obtained from wild sika deer and domestic cattle in Hokkaido, Japan. Parasitology international: 66, 519-521.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547-1549.
Le T, Blair D, McManus DP (2001) Complete DNA sequence and gene organization of the mitochondrial genome of the liverfluke, Fasciola hepatica L. (Platyhelminthes; Trematoda). Parasitology 123: 609-621.
Maddison DR, Swofford DL, Maddison WP (1997) NEXUS: an extensible file format for systematic information. Systematic Biology 46: 590-621.
Mage C, Bourgne H, Toullieu JM, Rondelaud D, Dreyfuss G (2002) Fasciola hepatica and Paramphistomum daubneyi: changes in prevalences of natural infections in cattle and in Lymnaea truncatula from central France over the past 12 years. Veterinary Research 33: 439-447.
Mas-Coma S (2003) Adaptation capacities of Fasciola hepatica and their relationships with human fascioliasis: from below sea level up to the very high altitude. Taxonomy, Ecology and Evolution of Metazoan Parasites 2: 81-123.
Mas-Coma S (2005) Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology 79: 207-216.
Mas-Coma S, Bargues MD, Valero M (2005) Fascioliasis and other plant-borne trematode zoonoses. International Journal for Parasitology 35: 1255-1278.
Mas‐Coma S, Valero MA, Bargues MD (2009) Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology 69: 41-146.
McCann C, Baylis M, Williams D (2010) Seroprevalence and spatial distribution of Fasciola hepatica-infected dairy herds in England and Wales. Veterinary Record 166: 612-617.
Mehmood K, Zhang H, Sabir AJ, Abbas RZ, Ijaz M, Durrani AZ, Saleem MH, Rehman MU, Iqbal MK, Wang Y (2017) A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants. Microbial Pathogenesis 109: 253-262.
Mekroud A, Benakhla A, Vignoles P, Rondelaud D, Dreyfuss G (2004) Preliminary studies on the prevalences of natural fasciolosis in cattle, sheep, and the host snail (Galba truncatula) in north-eastern Algeria. Parasitology Research 92: 502-505.
Mendes E, Lima W, De Melo A (2008) Development of Fasciola hepatica in Lymnaea columella infected with miracidia derived from cattle and marmoset infections. Journal of Helminthology 82: 81-84.
Nyindo M, Lukambagire AH (2015) Fascioliasis: an ongoing zoonotic trematode infection. BioMed Research International 786195.
Ouchene-Khelifi N, Ouchene N, Dahmani H, Dahmani A, Sadi M, Douifi M (2018) Fasciolosis due to Fasciola hepatica in ruminants in abattoirs and its economic impact in two regions in Algeria. Tropical Biomedicine 35: 181-187.
Ramos-Onsins SE, Rozas J (2002) Statistical properties of new neutrality tests against population growth. Molecular Biology and Evolution 19: 2092-2100.
Rinaldi L, Biggeri A, Musella V, De Waal T, Hertzberg H, Mavrot F, Torgerson PR, Selemetas N, Coll T, Bosco A (2015) Sheep and Fasciola hepatica in Europe: the GLOWORM experience. Geospatial Health 9: 309-317.
Robinson MW, Dalton JP (2009) Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases. Philosophical Transactions of the Royal Society B: Biological Sciences 364: 2763-2776.
Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, Guirao-Rico S, Librado P, Ramos-Onsins SE, Sánchez-Gracia A (2017) DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution 34: 3299-3302.
Salzburger W, Ewing GB, Von Haeseler A (2011) The performance of phylogenetic algorithms in estimating haplotype genealogies with migration. Molecular Ecology, 20: 1952-1963.
Schwantes J, Quevedo P, D’Ávila M, Molento M, Graichen D (2020) Fasciola hepatica in Brazil: genetic diversity provides insights into its origin and geographic dispersion. Journal of Helminthology 94: E83.
Schweizer G, Plebani G, Braun U (2003) Prevalence of Fasciola hepatica and Dicrocoelium dendriticum in the cow: inspection in an east Switzerland abattoir. Schweizer Archiv fur Tierheilkunde 145: 177-179.
Semyenova SK, Morozova EV, Chrisanfova GG, Gorokhov VV, Arkhipov IA, Moskvin AS, Movsessyan SO, Ryskov AP (2006) Genetic differentiation in eastern European and western Asian populations of the liver fluke, Fasciola hepatica, as revealed by mitochondrial nad1 and cox1 genes. Journal of Parasitology 92: 525-530.
Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123: 585-595.
Walker S, Johnston C, Hoey E, Fairweather I, Borgsteede F, Gaasenbeek C, Prodöhl P, Trudgett A (2011) Population dynamics of the liver fluke, Fasciola hepatica: the effect of time and spatial separation on the genetic diversity of fluke populations in the Netherlands. Parasitology 138: 215-223.
Walker S, Prodöhl P, Fletcher H, Hanna R, Kantzoura V, Hoey E, Trudgett A (2007) Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep. Parasitology Research 101: 117.