Oxidant/Antioxidant Status and Certain Trace Elements Relationship in Hair Goats Naturally Infected by Neospora caninum

PDF (English)
Publiée : Apr 17, 2023
Mis à jour : 2023-04-17
Versions :
2023-04-17 (3)
DOI : https://doi.org/10.12681/jhvms.27283
BA Çelik
Siirt University
https://orcid.org/0000-0002-0130-970X
ÖY Çelik
Siirt University
https://orcid.org/0000-0001-6365-2688
K İrak
Siirt University
https://orcid.org/0000-0001-9765-0330
M Bolacalı
Siirt University
https://orcid.org/0000-0002-4196-2359
Résumé

This study was performed to determine the prevalence of Neospora caninum in female hair goats raised in different locations of the Siirt province of Turkey, and to investigate the total antioxidant status (TAS), total oxidant status (TOS), Zinc (Zn), and Copper (Cu) levels of the infected goats. The animal material of the study consists of a total of 184 female hair goats that are between 1 to 6 years old with the random sampling method. Investigation of the N.caninum antibodies was then performed using a commercial ELISA kit. The results of the ELISA tests reveal that the prevalence of N. caninum in the province of Siirt was 10.33% (19/184) positive, 7.61% (14/184) suspected, and 82.07% (151/184) negative. According to the ELISA test results, 10 seropositive and 10 seronegative sera samples were used in the TAS, TOS, Zn, and Cu analysis.  The TAS (p<0.01) and Zn (p<0.001) levels of goats positive with N. caninum were found to be lower compared to those of the seronegative goats, while their TOS and Cu levels were higher (p<0.05). This study was the first to determine the N.caninum prevalence in the goats raised in the province of Siirt. It was concluded that it would be beneficial to take precautionary measures, as well as further studies with larger scopes to be performed which should also involve dogs as the final hosts. Furthermore, the oxidative stress parameters (TAS, TOS) and certain trace elements (Zn, Cu) were found to be affected by the Neosporosis, which should be useful in the diagnosis and treatment of the disease.

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Akış ME, and Dede S (2009) Babesiosisli koyunlarda çinko ve bakır konsantrasyonları ve karbonik anhidraz enzim aktivitesinin saptanması. Van Vet J, 20, 33-37.
Al-Majali A, Jawasreh K, Talafha H, and Talafha A (2008) Neosporosis in sheep and different breeds of goats from Southern Jordan: prevalence and risk factors analysis. Am J Anim Vet Sci, 3, 47-52.
Anastasia D, Elias P, Nikolaos P, Charilaos K, and Nektarios G (2013) Toxoplasma gondii and Neospora caninum seroprevalence in dairy sheep and goats mixed stock farming. Vet. Parasitol., 198, 387-390.
Ayaz E, Ertekin A, Ozdal N, and Taş Z (2007) Some biochemical parameters in sheep infected with endoparasites (Fasciola spp., Dicrocoelium dendriticum, hydatid cysts, Trichostrongylidae and Protostrongylidae). Turkiye Parazitol Derg, 31, 57-61.
Bartova E, and Sedlak K (2012) Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic. Vet. Med, 57, 111-114.
Bottari NB, Tonin AA, Fighera R, Flores MM, França RT, Camillo G, Toscan G, Vogel FS, Sangoi MB, and Bochi GV (2014) Neospora caninum and Toxoplasma gondii: relationship between hepatic lesions, cytological and biochemical analysis of the cavitary liquid during the acute phase of the diseases in experimental models. Exp. Parasitol., 136, 68-73.
Cayvaz M, and Karatepe M (2011) Niğde Yöresi Keçilerinde Neospora caninum’un Seroprevalansı. Kafkas Univ Vet Fak Derg, 17, 935-939.
Chaturvedi U, Shrivastava R, and Upreti R (2004) Viral infections and trace elements: a complex interaction. Curr. Sci, 87, 1536-1554.
Costanzo L, De Guidi G, Giuffrida S, Sortino S, and Condorelli G (1995) Antioxidant effect of inorganic ions on UVC and UVB induced lipid peroxidation. J. Inorg. Biochem, 59, 1-13.
Czopowicz M, Kaba J, Szaluś-Jordanow O, Nowicki M, Witkowski L, and Frymus T (2011) Seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Poland. Vet. Parasitol., 178, 339-341.
Da Silva Andrade G, Bruhn FRP, Rocha CMBM, de Sá Guimarães A, Gouveia AMG, and Guimarães AM (2013) Seroprevalence for Neospora caninum in goats of Minas Gerais state, Brazil. Res. Vet. Sci., 94, 584-586.
Değer S, Biçek K, and Değer Y (2005) Theileriosis' li Sığırlarda Bazı Biyokimyasal Parametrelerdeki (Demir, Bakır, Vitamin C ve Vitamin E) Değişiklikler. Van Vet J, 16, 49-50.
Díaz P, Cabanelas E, Díaz JM, Viña M, Béjar JP, Pérez-Creo A, Prieto A, López CM, Panadero R, and Fernández G (2016) Seroprevalence of Toxoplasma gondiiand Neospora caninumin goats from north-western Spain. Ann. Agric. Environ. Med., 23, 587-590.
Dubey J (2003) Review of Neospora caninum and neosporosis in animals. Korean J. Parasitol, 41, 1-16.
Dubey J, Schares G, and Ortega-Mora L (2007) Epidemiology and control of neosporosis and Neospora caninum. Clin Microbiol Rev, 20, 323-367.
Erel O (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem, 37, 277-285.
Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clin. Biochem, 38, 1103-1111.
Figliuolo LPC, Rodrigues A, Viana R, Aguiar DMd, Kasai N, and Gennari SM (2004) Prevalence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in goat from São Paulo State, Brazil. Small Rumin Res, 55, 29-32.
Gazzonis AL, Garcia GA, Zanzani SA, Mora LMO, Invernizzi A, and Manfredi MT (2016) Neospora caninum infection in sheep and goats from north-eastern Italy and associated risk factors. Small Rumin Res, 140, 7-12.
Gharekhani J, Esmaeilnejad B, Rezaei H, Yakhchali M, Heidari H, and Azhari M (2016) Prevalence of anti-Neospora caninum antibodies in Iranian goats. Ann Parasitol, 62.
Gos ML, Manazza JA, Späth EJ, Pardini L, Fiorentino MA, Unzaga JM, Moré G, and Venturini MC (2017) Seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats from two Argentinean provinces. Open J Vet, 7, 319-322.
Har U, and Başbuğan Y Van İli Gevaş İlçesinde Atık Yapan Koyunlarda Neospora Caninum’un Seroprevalansi. Van Sağlık Bilimleri Dergisi, 12, 6-12.
Hucker D, and Young W (1986) Effects of concurrent copper deficiency and gastro-intestinal nematodiasis on circulating copper and protein levels, liver copper and bodyweight in sheep. Vet. Parasitol., 19, 67-76.
Iovu A, Györke A, Mircean V, Gavrea R, and Cozma V (2012) Seroprevalence of Toxoplasma gondii and Neospora caninum in dairy goats from Romania. Vet. Parasitol., 186, 470-474.
Irak K, Aslan-Celik B, Karakoc Z, Celik O, Mert H, Mert N, and Kaya M (2018) Oxidant/Antioxidant Status, PON1 and ARES Activities, Trace Element Levels, and Histological Alterations in Sheep with Cystic Echinococcosis. Iran J Parasitol, 13, 448.
Liu Z-K, Li J-Y, and Pan H (2015) Seroprevalence and risk factors of Toxoplasma gondii and Neospora caninum infections in small ruminants in China. Prev Vet Med, 118, 488-492.
Miller J, Brzezinska-Slebodzinska E, and Madsen F (1993) Oxidative stress, antioxidants, and animal function. J. Dairy Sci., 76, 2812-2823.
Moraes LMdB, Raimundo JM, Guimarães A, Santos HA, Macedo Junior GdL, Massard CL, Machado RZ, and Baldani CD (2011) Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies in goats and sheep in western Maranhão, Brazil. Rev. Bras. Parasitol. Vet., 20, 312-317.
Nasir A, Ashraf M, Khan M, Javeed A, Yaqub T, Avais M, and Reichel M (2012) Prevalence of Neospora caninum antibodies in sheep and goats in Pakistan. J. Parasitol., 98, 213-215.
Sahin T, and Akgül Y (2006) Investigation of some trace element levels and biochemical parameters in sheep with endoparasite. YYÜ Sağlık Bilimleri Dergisi, 9, 100-106.
Sevgili M, Çimtay İ, and Keskin O (2003) Şanlıurfa yöresindeki keçilerde Neospora caninum enfeksiyonunun seroprevalansı. Türkiye Parazitol Derg, 27, 249-251.
Seyrek K, Pasa S, Kiral F, Bildik A, and Kilic S (2004) Levels of zinc, copper and magnesium in sheep with toxoplasmosis. Uludag Univ. J. Fac. Vet. Med., 23, 39-42.
Sharma RN, Bush J, Tiwari K, Chikweto A, and Bhaiyat MI (2015) Seroprevalence of Neospora caninum in sheep and goats from Grenada, West Indies. Open J. Vet. Med, 5, 219.
Sun L-X, Liang Q-L, Nie L-B, Hu X-H, Li Z, Yang J-F, Zou F-C, and Zhu X-Q (2020) Serological evidence of Toxoplasma gondii and Neospora caninum infection in black-boned sheep and goats in southwest China. Parasitol. Int., 75, 102041.
Taşkapan Ç, Atambay M, Aycan ÖM, Özyalin F, Yoloğlu S, Miman Ö, and Daldal N (2007) Giardiosisli hastalarda serum çinko (Zn) düzeyleri. Türk Parazitol Derg, 31, 14-16.
Tonin A, Weber A, Ribeiro A, Camillo G, Vogel F, Moura A, Bochi G, Moresco R, and Da Silva A (2015) Serum levels of nitric oxide and protein oxidation in goats seropositive for Toxoplasma gondii and Neospora caninum. Comp. Immunol. Microbiol. Infect. Dis., 41, 55-58.
Topazio JP, Weber A, Camillo G, Vogel FF, Machado G, Ribeiro A, Moura AB, Lopes LS, Tonin AA, and Soldá NM (2014) Seroprevalence and risk factors for Neospora caninum in goats in Santa Catarina state, Brazil. Rev. Bras. Parasitol. Vet., 23, 360-366.
Utuk A, and Eski F (2017) Detection of anti-Neospora caninum antibodies in a goat flock in Kilis province of Turkey. IJVS, 6, 114-117.
Utuk A, and Eski F (2019) Investigation of anti-Neospora caninum antibodies and disease-related risk factors in goats. Med. Weter., 75, 678-683.
Utuk A, Simsek S, Piskin F, and Balkaya I (2011) Detection of Neospora caninum IgG antibodies in goats in Elazig, Erzurum and Kırsehir Provinces of Turkey. Isr. J. Vet. Med, 66, 157-160.
Uzêda RS, Pinheiro AM, Fernández SY, Ayres MCC, Gondim LFP, and Almeida MAOd (2007) Seroprevalence of Neospora caninum in dairy goats from Bahia, Brazil. Small Rumin Res, 70, 257-259.
Van Weyenbergh J, Santana G, D'Oliveira A, Santos AF, Costa CH, Carvalho EM, Barral A, and Barral-Netto M (2004) Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study. BMC Infect. Dis., 4, 50.
Zhou M, Cao S, Sevinc F, Sevinc M, Ceylan O, Liu M, Wang G, Moumouni PFA, Jirapattharasate C, and Suzuki H (2016) Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey. J. Vet. Med. Sci., 78, 1877-1881.