Molecular diagnosis of peste des petits ruminants virus (PPR) in goats and sheep populations

PDF
Published: Nov 9, 2022
Updated: 2022-11-09
DOI: https://doi.org/10.12681/jhvms.28142
Keywords:
Peste des petits ruminants virus reverse transcriptase polymerase chain reaction diagnosis goats sheep
A Abdel-Rady
https://orcid.org/0000-0003-1361-2092
Abstract

Peste des petits ruminants (PPR) is an economically important viral disease of goats and sheep. The disease is confused clinically with other infections such as the mild strain of rinderpest in small ruminants. Effective control measures for PPR need that a proper and rapid diagnostic technique of disease. Therefore, the use of reverse transcriptase-polymerase chain reaction (RT-PCR) to detect suspected field samples collected from diseased goats and sheep in Dammam city, Kingdom of Saudia Arabia (KSA) has helped to give an effective diagnosis that was needed to control measure of the spread of the disease. This assay is based on the rapid purification of RNA on glass beads followed by the reverse transcription-polymerase chain reaction (RT-PCR). The primers (NP3/NP4) were used to amplify specifically a fragment of about 350 bp, that technique has a more specific and sensitive method for rapid diagnosis of disease.

Key words: Peste des petits ruminants virus; reverse transcriptase polymerase chain reaction; diagnosis; goats; sheep

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Abu-Elzein, E.M.E., Hassanien.M.M., AL-afaleq.A.I., Abdlhadi, F. M.
and Housawi F. M. T. (1990): Isolation of peste des petits ruminant
from goats in Saudi Arabia. Vet.Rec. 127:309-310.
Anderson, E.C., Hassan, A., Barrett, T., Anderson, J., 1990. Observation
on the pathogenicity for sheep and goats and the transmissibility of
the strain of virus isolated during the rinderpest outbreak in Sri Lanka
in 1987. Veterinary Microbiology 21, 309-318.
Asmar, J.A., Radwan, A.I., Abi Assi, N., and AL- Rashied A. A. (1980):
PPR like disease in sheep of central Saudi Arabia: Evidence of its
immunological relation to rinder pest; prospects for a control method.
Proceedings of the Saudi Biological Society, King Saud University
Press, Riyadh, Saudi Arabia.
Barrett T, Visser IKG, Mamaev L, Goatley L, van Bressem MF, Osterhaus
ADME (1993). Dolphin and porpoise morbilliviruses are genetically
distinct from phocine distemper virus. Virol. 193: 101-1012.
Brindha K, Dhinakar Raj G, Ganesan PI, Thiagarajan V, Nainar AM, Nachimuthu
K (2001). comparism of virus isolated and PCR for diagnosis
of PPR. Acta Virol. 45: 166-172.
Choi KS, Nah JJ, Choi CU, Ko YJ, Sohn HJ, Libeau G, Kang SY, Joo YS
(2003). Monoclonal antibody-based competitive ELISA for simultaneous
detection of rinderpest virus and peste des petits ruminants
virus antibodies. Vet. Microbiol. 96: 1-16.
Couacy-Hymann, E., Bidjeh, K., Angba, A., Domenech, J., Diallo, A.,
Goats vaccinated with the attenuated peste des petits ruminants
virus are protected against rinderpest disease. Research in Veterinary
Science 59, 106-109.
Couacy-Hymann E, Roger F, Hurard C, Guilou JP, Libeau G, Diallo A
(2002). Rapid and sensitive detection of PPRV by a PCR assay. J
Virol. Methods, 100: 17-25.
Dhar P, Screenivasa BP, Barrett T, Corteyn M, Singh RP, Bandyopadyay
SK (2002). Recent epidemiology of peste des petits ruminants virus.
Vet. Microbiol. 88: 153-159.
Diallo, A., Barrett, T., Barbron, M., Shaila, M.S., Taylor, W.P., 1989. Differentiation
of rinderpest and peste des petits ruminants viruses using
specific cDNA clones. Journal of Virological Methods 23, 127-136.
Diallo, A., Barrett, T., Barbron, M., Meyer, G., Lefevre, P.C., 1994. Cloning
of the nucleocapsid protein gene of peste des petits ruminants
virus: relationship to other Morbilliviruses. Journal of General Virology
, 233-237.
Diallo A, Libeau G, Couacy-Hymann E, Barbron M (1995). Recent developments
in the diagnosis of rinderpest and peste des petits ruminants.
Vet. Microbiol. 44: 307-317.
Forsyth M. and Barrett T. (1995). Evaluation of PCR for detection and
characterization of rinderpest and peste des petits ruminants viruses
for epidemiological studies. Virus Res. 39: 151-163.
Gibbs EPJ, Taylor WP, Lawman MJP, Bryant J (1979). Classification of
peste des petits ruminants virus as the fourth member of the genus
Morbillivirus. Intervirol. 11:268-274.
Govindarajan, R., Koteesswaran, A., Venugopalan, A.T., Shyam, G.,
Shaquna, S., Shaila, M.S., Ramachandran, S., 1997. Isolation of peste
des petits ruminants from an outbreak in Indian Buffalo Bubalus bubalis.
Veterinary Record 141, 573-574.
Hafez, S. M., A. AL sukayran, D. D. Cruz, S. I. Bakairi, A I. Radwan
(1987): An outbreak of rinder pest-like disease in gazelles in Saudi
Arabia. Symp. on the potentional of wildlife conservation in Saudi
Arabia. (Abstract), King Saud University Press, Riyadh, Saudi Arabia.
Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis
of rinderpest and peste des petits ruminants using an immunocapture
ELISA. Veterinary Record 134, 300-304.
Libeau, G., Diallo, A., Lancelot, R., Colas, F., Guerre, L., 1995. Development
of a competitive ELISA for detecting antibodies to the peste
des petits ruminants virus using a recombinant N protein. Research in
Veterinary Science 58, 50-55.
Mathew Z (24-26 September 1980). In: Proceedings of the International
Workshop pp.22-23, Held at IITA, Ibadan, Nigeria.
Mccaustland, K.A., BI, S., Purdy, M.A., Bradley, D.W., 1991. Application
of two RNA extraction methods prior to amplification of hepatitis E
virus nucleic acid by the polymerase chain reaction. Journal of Virological
Methods 35, 331-342.
McCullough J (1951). In: Eastern Region Nigeria Annual Report, Veterinary
Department, 1949-1950. p.27.
Mornet P, Orue J, Gilbert Y, Thiery G , Mamadou SOW (1956). La peste
des petits ruminants on Afrique occidentale francaise et ses rapports
avec la peste bovine, Rev. Elev. Med. Vet. Pays. Trop. 9: 313-342.
Nanda, Y.P., Chatterjee, A., Purohit, A.K., Diallo, A., Inui, K., Sharma,
R.N., Libeau, G., Thevasagayam, J.A., Bru¨nning, A., Kitching, R.P.,
Anderson, J., Barrett, T., Taylor, W.P., 1996. The isolation of peste des
petits ruminants virus from northern India. Veterinary Microbiology
, 207-216.
Nduaka O and Ihemelandu EC (1973). Observations on pneumoniaenteritis
complex in dwarf goats in Eastern Region of Nigerian -Preliminary
report. Bull.Epizoot. Dis. Afri. 21:89-98.
Obi TU, Durojaiye OA, Ojo MO, Taylor WP, Rowe LW (1983). Studies on the epidemiology of peste des petits ruminants in Southern Nigeria.
Trop. Vet.1: 209-217.
Obi TU, Patrick D. (1984). The detection of peste des petits ruminants
virus antigen by agar gel precipitation test and counterimmunoelectrophoresis.
Hyg. 93: 579-586.
Raj GD, Rajanathan TMC, Kumar CS, Ramathilagam G, Hiremath G,
Shaila MS (2008). Detection of peste de petits ruminants virus antigen
using immunofiltration and antigen-competition ELISA methods.
Vet. Microbiol. 129: 246-251.
Shaila, M.S., Purushothaman, V., Bhavasar, D., Venugopal, K., Venkatesan,
R.A., 1989. Peste des petits ruminants of sheep in India. Veterinary
Record 125, 602.
Shaila MS, Shamaki D, Forsyth MA, Diallo A, Goatley L, Kitching P,
Barrett T (1996). Geographic distribution and epidemiology of Peste
des Petits Ruminants Viruses. Vir. Res. 43: 149-153.
Steinhauer DA, De La Torre JC, Holland JJ (1989). High nucleotide substitution
error frequencies in clonal pools of vesicular stomatitis virus.
J. Virol. 63: 2063-2071.
Taylor WP (1984). The distribution and epidemiology of peste des petits
ruminants. Prevent. Vet. Med. 2: 157-166.
Ularamu H. G., Owolodun, O. A. WomaT. Y.,. Audu B. J,. Aaron G. B,
Chollom S. C. and Shamaki D. (2012): Molecular diagnosis of recent
suspected outbreaks of peste des petits ruminants (PPR) in Yola, Adamawa
State, Nigeria. African Journal of Biotechnology Vol. 11(5),
pp. 1158-1162.
Yamada, O., Matsumoto, T., Nkashima, M., Hagari, S., Kamahora, T.,
Ueyama, H., Kishi, Y., Uemura, H., Kumrimura, T., 1990. A new
method for extracting DNA or RNA for polymerase chain reaction.
Journal of Virological Methods 27, 203-210.
Most read articles by the same author(s)