The supplementation of ovine oocyte maturation medium with triiodothyronine affects the embryo development and apoptotic gene expression

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DOI : https://doi.org/10.12681/jhvms.28514
R ASADPOUR
Department of Clinical Science, Faculty of VeterinaryMedicine, University of Tabriz, Tabriz, Iran
F AHMADNEJAD
Department of Clinical Science, Faculty of VeterinaryMedicine, University of Tabriz, Tabriz, Iran
L ROSHANGAR
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
A SABERIVAND
Department of Clinical Science, Faculty of VeterinaryMedicine, University of Tabriz, Tabriz, Iran
A HAJIBEMANI
Department of Clinical Science, Faculty of VeterinaryMedicine, University of Tabriz, Tabriz, Iran
Résumé

Triiodothyronine (T3) plays an essential role in different animal species’ embryonic development. The present research was designed to identify the effect of triiodothyronine on the in vitro ovine embryonic development and the expression of apoptotic genes.A total of 436 immature cumulus-oocyte complexes (COCs) were cultured for 24 h in the oocyte maturation medium supplemented with two concentrations of T3 (T-10 and T-100 ng/mL) or without T3(T-0: control group). Oocyte maturation, cleavage, and blastocyst rates were assessed under an inverted microscope as crucial indicators of embryo development.The relative mRNA abundance of BCL-2-associated X protein (BAX) and anti-apoptotic B-cell lymphoma-2 (BCL2) were determined at blastocysts (day 8 after IVF on day 0)by quantitative reverse transcription PCR.The data were analyzed by logistic regression using the GLIMMIX procedure followed by Chi-Square, and one-way ANOVA tests. The higher concentration of T3(100 ng/mL) significantly decreased cumulus expansion and blastocyst rate compared to controls (P<0.001). Additionally, a significantly higher expression level of BAX(P<0.001) and a dramatically lower expression level of BCL2 (P<0.01) were detected in the T-100ng/mL group compared to the controls.However, the relative mRNA level of BCL-2 was significantly higher in the T-10 ng/mL group compared to the control group (P<0.01).It appears that the supplementation of ovine oocyte maturation medium with T3 at high concentration (100 ng/mL) suppresses the ratio of blastocyst formation.

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Références
Alsalim H, Jafarpour F, TanhaeiVash N, Nasr-Esfahani MH, Niasari-Naslaji A (2018). Effect of DNA and histone methyl transferase inhibitors on outcomes of buffalo-bovine interspecies somatic cell nuclear transfer. Cell Reprogram 20(4):256-267.
Arias-Alvarez M, Bermejo-Alvarez P, Gutierrez-Adan A, Rizos D, Lorenzo PL, Lonergan P(2011).Effect of leptin supplementation during in vitro oocyte maturation and embryo culture on bovine embryo development and gene expression patterns. Theriogenology 75(5):887-896.
Asahara S, Sato A, Aljonaid AA, Maruo T (2003). Thyroid hormone synergizes with follicle stimulating hormone to inhibit apoptosis in porcine granulosa cells selectively from small follicles. Kobe J Med Sci 49(5/6):107-116.
Ashkar FA, Bartlewski PM, Singh J, Malik PS, Yates KM, Singh T, King WH (2010a). Thyroid hormone concentrations in peripheral circulation and ovarian follicular fluid of cows.ExpBiolMed 1-11.
Ashkar FA, Semple E, Schmidt CH, St. John E, Bartlewski PM, King WA (2010b). Thyroid hormone supplementation improves bovine embryo development in vitro. Hum Reprod 25(2):334-344.
Burlacu A (2003). Regulation of apoptosis by Bcl-2 family proteins. J Cell Mol Med 7(3):249-257.
Cecconi S, Rossi G, Coticchio G, Macchiarelli G, Borini A, Canipari R (2004). Influence of thyroid hormone on mouse preantral follicle development in vitro. FertilSteril 81(1): 919-924.
Cecconi S, Rucci N, Scaldaferri ML, Masciulli MP, Rossi G, Moretti C, D’ArmientoM,Ulisse S (1999). Thyroid hormone effects on mouse oocyte maturation and granulosa cell aromatase activity. Endocrinology 140(4):1783-1788.
Costa NN, Cordeiro MS, Silva TV, Sastre D, Santana PP, Sá AL, Sampaio RV, Santos SS, Andona PR, Miranda MS, Ohashi OM (2013). Effect of triiodothyronine on developmental competence of bovine oocytes. Theriogenology 80(4):295-301.
Davachi ND, Fallahi R, Dirandeh E, Liu X, Bartlewski PM (2018). Effects of co-incubation with conspecific ampulla oviductal epithelial cells and media composition on cryotolerance and developmental competence of in vitro matured sheep oocytes. Theriogenology 120: 10-15.
Detlaf TA, Davydova SI (1974). The effect of triiodothyronine on oocyte maturation in the starred sturgeon following exposure to low temperatures and female reservation. Ontogenez 5(5):454-462.
Di Francesco S, Boccia L, Campanile G, Di Palo R, Vecchio D, Neglia G, Zicarelli L,Gasparini B (2011). The effect of season on oocyte quality and developmental competence in Italian Mediterranean buffaloes (Bubalusbubalis). AnimReprodSci 123(1-2):48-53.
El-Raey M, Geshi M, Somfai T, Kaneda M, Hirako M, Abdel-Ghaffar AE,Sosa GA,El-Roos ME (2011). Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle. MolReprod Dev 78(4):250-262.
Feugang JM, Greene JM, Willard ST, Ryan PL (2011). In vitro effects of relaxin on gene expression in porcine cumulus-oocyte complexes and developing embryos. ReprodBiolEndocrinol9(1):1-0.
Filali M, Frydman N, Belot MP, Hesters L, Gaudin F, Tachdjian G, Emilie D, Frydman R, MachelonV (2009). Oocyte in-vitro maturation: BCL2 mRNA content in cumulus cells reflects oocyte competency. Reprod Biomed Online 19: 71-84.
Flood MR, Gage T, Bunch T (1993). Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro. Theriogenology 39(4):823-833.
Gardner DK, Michelle L (2003). Towards a single embryo transfer. Reprod Biomed Online 6(4):470-481.
Hashimoto S (2009). Application of in vitro maturation to assisted reproductive technology. J Reprod Dev 55:1-10.
Komninou ER, Remião MH, Lucas CG, Domingues WB, Basso AC, Jornada DS, Deschamp JC, Beck RC, Pohlmann AR, Bordigon V, Seixas FK(2016). Effects of two types of melatonin-loaded nanocapsules with distinct supramolecular structures: polymeric (NC) and lipid-core nanocapsules (LNC) on bovine embryo culture model. PloSone 11(6): e0157561.
Lane M, Gardner DK (2007). Embryo culture medium: which is the best? Res ClinObstetGynaecol 21(1):83-100.
Laoag-Fernandez JB, Matsuo H, Murakoshi H, Hamada AL, Tsang BK, Maruo T (2004). 3,5,3’-triiodothyronine down-regulates Fas and Fas ligand expression and suppresses caspase-3 and poly (adenosine 5’-diphosphate-ribose) polymerase cleavage and apoptosis in early placental extravillous trophoblasts in vitro. J Clin Endocrinol Metab 89(8):4069 - 4077.
Laskowski D, Sjunnesson Y, Humblot P, Sirard MA, Andersson G, Gustafsson H, Bage R (2017). Insulin exposure during in vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential. ReprodFertil Dev 29(5):876-889.
LivakKJ,Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method.Methods25(4), 402-408.
Marei WF, Wathes DC, Fouladi-Nashta AA (2009). The effect of linolenic acid on bovine oocyte maturation and development. BiolReprod 81(6):1064-1072.
Ni H, Sheng X, Cui X, Gu M, Liu Y, Qi X, Xing S, Guo Y(2015). Epidermal growth factor-mediated mitogen-activated protein kinase3/1 pathway is conducive to in vitro maturation of sheep oocytes. PLoS One. 10(3):e0120418.
Pers-Kamczyc E, Warzych E, Peippo J, Lechniak D (2010). Growth hormone exerts no effect on the timing of the first zygotic cleavage in cattle. Theriogenology 74(4):581-595.
Raine JC, Cameron C, Vijayan MM, Lamarre J, Leatherland JF (2004). The effect of elevated oocyte triiodothyronine content on development of rainbow trout embryos and expression of mRNA encoding for thyroid hormone receptors. J Fish Biol 65(1):206-226.
Rao BS, Mahesh YU, Charan KV, Suman K, Sekhar N, Shivaji S (2012). Effect of vitrification on meiotic maturation and expression of genes in immature goat cumulus oocyte complexes. Cryobiology 64:176-184.
Regassa A, Rings F, Hoelker M, Cinar U, Tholen E, Looft C, Schellander K, Tesfaye D(2011). Transcriptome dynamics and molecular crosstalk between bovine oocyte and its companion cumulus cells. BMC genomics 12(1):57.
Sechman A, Pawlowska K, Rzasa J (2009). Influence of triiodothyronine (T(3)) on secretion of steroids and thyroid hormone receptor expression in chicken ovarian follicles. DomestAnimEndocrinol 37(2):61-73.
Shi YB, Fu L, Hsia SC, Tomita A, Buchholz D (2001). Thyroid hormone regulation of apoptotic tissue remodelling during anuran metamorphosis. Cell Res 11(4):245 – 252.
Spicer LJ, Alonso J, Chamberlain CS (2001). Effects of thyroid hormones on bovine granulosa and thecal cell function in vitro: dependence on insulin and gonadotropins. J Dairy Sci 84(5):1069-1076.
Vissenberg R, Manders VD, Mastenbroek S, Fliers E, Afink GB, Ris-Stalpers C, Goddijn M, Bisschop PH (2015). Pathophysiological aspects of thyroid hormone disorders/thyroid peroxidase autoantibodies and reproduction. Hum Reprod Update. 21(3):378-387.
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