Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors Oxidative Stress in Sheep with Caseous Lymphadenitis


E Polat
https://orcid.org/0000-0002-3999-1310
E Kaya
B Karagülle
H Akin
Résumé

In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the early diagnosability of superficial and visceral caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep isolated from C. pseudotuberculosis were compared with healthy sheep, it was determined that there was a statistically significant decrease in the levels of antioxidant molecules such as GSH-pX (14.62%), GSH (23.81%), SOD (4.70%), CAT (22.23%) (p˂0.001). The level of toxic malondialdehyde (MDA) (18.62%), the end product of lipid peroxidation, was found to be statistically significantly increased in sheep isolated from C. pseudotuberculosis (p˂0.001). As a result, it was determined that oxidative stress factors showed statistically significant variability in cases of superficial caseous lymphadenitis (caused by C.pseudotuberculosis). For this reason, by determining the levels of oxidative stress factors in suspected herds, it was possible to make early diagnosis of the superficial and visceral forms of caseous lymphadenitis, and a basis was established for future studies.

Article Details
  • Rubrique
  • Research Articles
Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Biographie de l'auteur
E Polat, Firat University, Faculty of Veterinary Medicine, Department of Surgery, Elazig, Turkey

Veteriner Cerrahisi

Références
Aebi H (1984) Catalase. In vitro. Methods in Enzymol 105: 121-126.
Akgul G, Kahya Demirbilek S, Çelik ÖY, Irak K, Akgul MB, Mersin S
(2018) Corynebacterium pseudotuberculosis Case in a Boer Goat X
Turkish Hair Goat Crossbred. Erciyes Üniv Vet Fak Derg 15(1): 82
Al-Gaabary MH, El-Sheikh WMA (2002) Epidemiological, clinical and
preventive studies on caseous lymphadenitis in sheep and goats at
Gharbia governorate. In: 10th Sci. Cong. Fac. Vet. Med. Assiut Uni
versity, Egypt, 402-417.
Al-Gaabary MH, Osman SA, Oreiby AF (2009) Caseous lymphadenitis
in sheep and goats: Clinical, epidemiological and preventive studies.
Small Rumin Res 87: 116-121. https://doi.org/10.1016/j.smallrum
res.2009.10.008
Baird GJ, Fontaine MC (2007) Corynebacterium pseudotuberculosis and
its role in ovine caseous lymphadenitis. J Comp Path 137: 179-210.
Basbug O, Tuzcu N, Ercan N, Aydogdu U, Ograk YZ (2014) Serum vi
tamin D levels in sheep with caseous lymphadenitis. FÜ Sağ Bil Vet
Derg 28(2): 77-80.
Bastos BL, Portela RWD, Dorella FA, Riberio D, Seyffert N, Castrao TLP,
Miyoshi A, Oliveria SC, Meyer R, Azevedo V (2012) Corynebacteri
um pseudotuberculosis: Immunological responses in animal models
and zoonotic potential. J Clin Cell Immunol S4: 1-15.
Beutler E (1984) Red cell metabolism. A manual of biochemical methods,
nd ed. Pp. 160, Grune and Starton, New York.
Bozukluhan K, Merhan O, Kızıltepe S, Harmankaya A, Gökçe G (2020)
Determination of oxidative stress and ceruloplasmin levels in sheep
with toxoplasmosis. Van Vet J 31(2): 83-86. https://doi.org/10.36483/
vanvetj.646976
Cetinkaya B, Karahan M, Atil E, Kalin R, De Baere T, Vaneechoutte M
(2002) Identification of Corynebacterium pseudotuberculosis isolates
from sheep and goats by PCR. Vet Microbiol 88(1): 75-83. https://doi.
org/10.1016/s0378-1135(02)00089-5
Dorella FA, Pacheco LGC, Olivera SC, Miyashi A, Azevedo V (2006)
Corynebacterium pseudotuberculosis: microbiology, biochemical
properties, pathogenesis and molecular studies of virulence. Vet Res
Ellman GL, Courtney KD, Andres V, Featherstone RM (1961) A new
and rapid colorimetric determination of acetylcholinesterase activ
ity. Biochem Pharmacol 7: 88-95. http://dx.doi.org/10.1016/0006
(61)90145-9
Ertas F, Kirmizigul AH (2021) Investigation of oxidative stress and met
abolic profile in sheep with fascioliasis. Atatürk Üniversitesi Vet Bil
Esmaeilnejad B, Tavassoli M, Asri-Rezaei S, Dalir-Naghadeh B, Maleki
nejad H, Jalilzadeh-Amin G, Arjmand J, Golabi M, Hajipour N (2014)
Evaluation of antioxidant status, oxidative stress and serumtrace min
eral levels associated with Babesia ovis parasitemiain sheep. Vet Par
Fontaine MC, Baird GJ (2008). Caseous lymphadenitis. Small Rumin Res
Frankel S, Reitman S, Sonnen AC (1970) A textbook on laboratory proce
dure and their interpretation. Ch 10. Grand-Wohl’s Clinical Labora
tory Methods and Diagnosis. London. The CV Mosby Co, 403-404.
Ilhan Z (2020) In vitro antimicrobial susceptibility of corynebacterium
pseudotuberculosis isolated from sheep with caseous lymphadenitis.
Kocatepe Vet J 13(2): 267-271. https://doi.org/10.30607/kvj.738662
Ilhan FS (2008). Pathological observation on caseous lymphadenitis in
fection in sheep. YYÜ Vet Fak Derg 19(1): 23-28.
Ipek V, Akgül O, Kahraman MM, Öztürkoglu SI, Büyükcangaz E, Me
citoglu Z, Yılmazbaş Mecitoglu G (2012) Internal form of caseous
lymphadenitis in a merinos sheep. Uludag Univ J Fac Vet Med 31(2):
-76.
Izgür M, Akan M, Ilhan Z (1999) Microorganisms isolated from cases of
caseous lymphadenitis. Ankara Univ Vet Fak Derg 46: 43-50.
Kumar J, Singh F, Tripathi BN, Kumar R, Dixit SK, Sonawane GG (2012)
Epidemiological, bacteriological and molecular studies on caseous
lymphadenitis in Sirohi goats of Rajasthan. Trop Anim Health Pro 44
Mubarak M, Bastawrows AF, Abdel-Hafez MM, Ali MM (1999). Caseous
lymphadenitis of sheep and goats in assıut farms and abattoırs. Assiut
Vet Med J 42: 89-107.
Oreiby AF (2015). Diagnosis of caseous lymphadenitis in sheep and goat.
Small Rumin Res 125: 160-166. http://dx.doi.org/10.1016/j.small
rumres.2014.11.013
Parin U, Kırkan Ş, Ural K, Savaşan S, Erbaş G, Gültekin M, Yüksel
HT, Balıkçı C (2018) Molecular identification of Corynebacteri
um pseudotuberculosis in sheep. Acta Vet Brno 87: 3-8. https://doi.
org/10.2754/avb201887010003
Placer ZA, Cushman L, Johnson BC (1966) Estimation of products of
lipid peroxidation in biological fluids. Anal Biochem 16: 359-364.
Polat E, Han MC, Yılmaz S, Kaya E, Kayapinar SD, Coskun S, Yildirim
A, Can UK (2021) The effect of hip dysplasia on some biochemical
parameters, oxidative stress factors and hematocrit levels in dogs. Pol
J Vet Sci 24(4): 473-478. https://doi.org/10.24425/pjvs.2021.139971
Robaj A, Hamidi A, Bytyqi H, Sylejmani D (2017) Frequency and antimi
crobial susceptibility of bacterial isolates from caseous lymphadenitis
in sheep in Kosovo. Bulg J Agric Sci 23(6): 1033-1036.
Sá Guimarães A, Borges do Carmo F, Pauletti RB, Seyffert N, Ribeiro
D, Lage AP, Heinemann MB, Miyoshi A, Azevedo V, Gouveia AMG
(2011) Caseous lymphadenitis: epidemiology, diagnosis, and control.
The IIOAB Journ 2(2): 33-43.
Seyranoglu K, Aslan Ö (2015) Effects of vitamin e and selenium com
bination on oxidative response in lambs vaccinated against caseous
lymphadenitis. Atatürk Üniversitesi Vet Bil Derg 10(3): 171-178.
Sheikh-Omar AR, Shah M (1984) Caseous lymphadenitis in sheep im
ported from Australia for slaughter in Malasia. Aust Vet J 61(12):
Slotte JP, Ramstedt B (2007) The functional role of sphingomyelin in
cell membranes. Eur J Lipid Sci Technol 109: 977-981. https://doi.
org/10.1002/ejlt.200700024
Songer JG (1997) Bacterial phospholipases and their role in virulence.
Trends Microbiol 5(4): 156-161. https://doi.org/10.1016/S0966
X(97)01005-6.
Sun Y, Oberly LW, Ying LA (1988) Simple method for clinical assay of su
peroxide dismutase. Clin Chem 34: 497-500. https://doi.org/10.1093/
clinchem/34.3.497
Tolu C, Savas T (2014) Variation in the frequency of superficial abscesses
based on the caseous lymphadenitis (CLA) disease in goats. Hayvan
sal Üretim 55(1): 19-24. https://doi.org/10.29185/hayuretim.363903
Williamson LH (2001) Caseous lymphadenitis in small ruminants. Vet
Clin N Am: Food Anim Pract 17: 359-371. https://doi.org/10.1016/
s0749-0720(15)30033-5
Windsor PA (2011) Control of caseous lymphadenitis. Vet Clin Food
Zaitoun AM, Bayoumi AH (1994) Some epidemiological studies on
ovine pseudotuberculosis. Assiut Vet Med J 31: 238-250. https://doi.
org/10.21608/AVMJ.1994.185364
Articles les plus lus par le même auteur ou la même autrice