Evaluation of Eosinophilic Myositis in a Bull: Histopathological and Immunohistochemical Findings

PDF (English)
Publiée : gen 15, 2025
DOI : https://doi.org/10.12681/jhvms.35052
A Milletsever
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, Burdur, Turkey
O Ozmen
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, Burdur, Turkey
https://orcid.org/0000-0002-1835-1082
Résumé

In this case report, histopathological and immunohistochemical findings of eosinophilic myositis (EM) observed in the gluteal muscles of a 15-month-old Holstein bull are presented. Macroscopically, multifocally distributed distinct gray-greenish demarcated foci were observed. Microscopic examinations revealed an inflammatory reaction with predominance of eosinophil granulocytes accompanied by mononuclear inflammatory cell infiltrations among myofibrils and increased connective tissue. Luna staining confirmed the presence of intracytoplasmic red granules in eosinophil leukocytes. Numerous remnants of sarcocyst cysts were found within the myofibers. Immunohistochemical examinations demonstrated increased expression of TNF-α, MMP-2, MMP-7, Cas-3, and IL-3. The findings of this study indicated the significant roles of cytokines, matrix metalloproteinases, and apoptosis in the development of eosinophilic myositis.

Article Details
  • Rubrique
  • Case Report
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Références
Alameddine HS (2012) Matrix metalloproteinases in skeletal muscles:
Friends or foes?. Neurobiol Dis 48(3):508-18.
Atasever A (1998) Eosinophilic myositis in a Cattle. Tr. J. of Veterinary
and Animal Sciences 22(1):93-96.
Chen W, Zhou Z, Chen D, Li Y, Zhang Q, Su J (2021) Bone regeneration
using MMP-cleavable peptides-based hydrogels. Gels 7(4):199.
Chen X, Li Y (2009) Role of matrix metalloproteinases in skeletal muscle:
migration, differentiation, regeneration and fibrosis. Cell Adh Migr
(4):337-41.
Collins RA, Grounds MD (2001) The role of tumor necrosis factor-alpha
(TNF-α) in skeletal muscle regeneration: studies in TNF-α (-/-) and
TNF-α (-/-)/LT-α (-/-) mice. J Histochem Cytochem 49(8):989-1001.
Cooper BJ, Valentine BA (2016) Muscle and tendon. In: Jubb, Kennedy,
and Palmer’s Pathology of Domestic Animals. Vol. 1, 6th ed, Saunders
Ltd, Philadelphia: pp 236-237.
Dini FM, Caffara M, Jacinto JG, Benazzi C, Gentile A, Galuppi R (2023)
A case of bovine eosinophilic myositis (BEM) associated with co-infection
by Sarcocystis hominis and Toxoplasma gondii. Animals (Basel)
(2):311.
Do SH, Jeong da H, Chung JY, Park JK, Yang HJ, Yuan DW, Jeong KS
(2008) Eosinophilic myositis in slaughtered Korean native cattle. J
Vet Sci 9(4):425-427.
Dubey JP, Rosenthal BM (2023) Bovine sarcocystosis: Sarcocystis species,
diagnosis, prevalence, economic and public health considerations,
and association of Sarcocystis species with eosinophilic myositis
in cattle. Int J Parasitol 53(9):463-475.
El-Beshbishi SN, Ahmed NN, Mostafa SH, El-Ganainy GA (2012) Parasitic
infections and myositis. Parasitol Res 110:1-18.
Esnault S, Kelly ES, (2016). Essential mechanisms of differential activation
of eosinophils by IL-3 compared to GM-CSF and IL-5. Crit Rev
Immunol 36(5):429-444.
Fermon C, Authier FJ, Gallay L (2022) Idiopathic eosinophilic myositis:
a systematic literature review. Neuromuscul Disord 32(2):116-124.
Fingleton B (2017) Matrix metalloproteinases as regulators of inflammatory
processes. Biochim Biophys Acta Mol Cell Res 1864(11):2036-
Gigon L, Fettrelet T, Yousefi S, Simon D, Simon HU (2023) Eosinophils
from A to Z. Allergy 78(7):1810-1846.
Kankaanranta H, Ilmarinen P, Zhang X, Adcock IM, Lahti A, Barnes
PJ, Giembycz MA, Lindsay MA, Moilanen E (2014) Tumour necrosis
factor-α regulates human eosinophil apoptosis via ligation of
TNF-receptor 1 and balance between NF-κB and AP-1. PloS One 9(2):e90298.
Kumar L, Bisen M, Khan A, Kumar P, Kumar P, Patel SKS (2022) Role of
matrix metalloproteinases in musculoskeletal diseases. Biomedicines
(10):2477.
Kuscher A (1940) Eosinophile Muskelentzündung beim Hund. Wien.
Tierärztl. Mschr 27:pp 177-186.
Lee HJ, Lee EK, Seo YE, Shin YH, Kim HS, Chun YH, Yoon JS, Kim
HH, Han MY, Kim CK, Kim KE, Koh YY, Kim JT (2017) Roles of
Bcl-2 and caspase-9 and-3 in CD30-induced human eosinophil apoptosis.
J Microbiol Immunol Infect 50(2):145-152.
Luna LG. Manual of histologic staining methods of the armed forces institute
of pathology. 3rd Ed.. McBrien CN, Menzies-Gow A (2017)
The biology of eosinophils and their role in asthma. Front Med (Lausanne)
:93.
Mittal R, Patel AP, Debs LH, Nguyen D, Patel K, Grati MH, Mittal J, Yan
D, Chapagain P, Liu XZ (2016) Intricate functions of matrix metalloproteinases
in physiological and pathological conditions. J Cell
Physiol 231(12):2599-2621.
New York: McGraw-Hill; 1968. p. 111-112.
Nissinen L, Kähäri VM (2014) Matrix metalloproteinases in inflammation.
Biochim Biophys Acta 1840(8):2571-2580.
Rhee SH, Yu IJ, Kim JH, Kwon JK, Park J, You MJ, Lee JW, Park HJ,
Chekarova I, Camer GA, Lim CW, Kim B (2008) Idiopathic eosinophilic
myositis in Korean native cattle (Bos taurus coreanae). Korean
J Vet Res (2008) 48(2):187-190.
Selva-O’Callaghan A, Trallero-Araguás E, Grau JM (2014) Eosinophilic
myositis: an updated review. Autoimmun Rev 13(4-5):375-8.
Sierra MA, Gomez MA, Navarro JA, Bernabe A (2008) Bovine eosinophilic
myositis caused by trematodes. Korean J Vet Res 48(2):187-
Simon D, Simon HU (2007) Eosinophilic disorders. J Allergy Clin Immunol
(6):1291-1300.
Smith HJ, Snowdon KE, Finley GG (1991) Eosinophilic myositis in Canadian
cattle. Can J Vet Res 55(1):94-5.
van Loo G, Bertrand MJ (2023) Death by TNF: a road to inflammation.
Nat Rev Immunol 23(5):289-303.
Vangeel L, Houf K, Geldhof P, Nollet H, Vercruysse J, Ducatelle R, Chiers
K (2012) Intramuscular inoculation of cattle with Sarcocystis antigen
results in focal eosinophilic myositis. Vet Parasitol 183(3-4):224-30.
Articles les plus lus par le même auteur ou la même autrice