Meta-analysis of association between Insulin-like growth factor-2 gene polymorphisms and growth traits in rabbits

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Publiée : Dec 18, 2025
DOI : https://doi.org/10.12681/jhvms.40709
HΑ Bashiru
Department of Animal Sciences, Faculty of Agriculture, Obafemi Awolowo University, Ile-Ife, Nigeria
https://orcid.org/0000-0001-7080-1012
SO Oseni
Department of Animal Sciences, Faculty of Agriculture, Obafemi Awolowo University, Ile-Ife, Nigeria
https://orcid.org/0000-0002-3060-4302
Résumé

The Insulin-like growth factor-2 (IGF-2) gene is a potential candidate for marker assisted selection in rabbits due to its crucial role in regulating growth. However, the relationship between polymorphic variants of this gene and growth performance traits of rabbits under different genetic models has not been established. Therefore, the objective of this study was to assess the association between IGF-2 gene polymorphisms and growth performance traits in rabbits using meta-analysis. A systematic review was conducted following the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) guideline to identify and select relevant studies for inclusion in the analysis. Data were extracted from nine reports and included in the final analysis. Six genetic models under the assumption of co-dominance (3 models), recessive (1 model), dominance (1 model) and over-dominance (1 model) were evaluated. SPSS software was used for the assessment of heterogeneity, effect size calculation using hedges g method for standardized mean differences (SMDs) and the assessment of publication bias, while sensitivity analysis was conducted using OpenMeta® Analyst software. High heterogeneity was observed across studies under all genetic models. In addition, there were significant associations (P<0.05) between of IGF-2 gene with growth performance traits of rabbits. A simple relationship between these genotypes for growth traits of rabbits could be stated as Del/Del > A/Del >A/A. The findings infer the superiority of the Del allele over the A allele (Del>A) for growth performance traits of rabbits. These findings implied that the IGF-2 gene could be a potential candidate gene for marker assisted selection of rabbits for improved growth performance.

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Références
Abdel-Kafy EM, Gafer JA, Shaaban HMA (2014) Impact of insulin-like
growth factor-II polymorphisms on growth and reproductive traits
in rabbits. Arab J Biotechnol 17:121–132.
Ampaporn K, Suwannasing R, Phongphanich P, Tunim S, Duangjinda
M (2023) The variation of insulin-like growth factor 2 marker is
associated with growth traits in Thai native (Kradon) pigs. Anim
Biosci 36:1350–1356.
Aslan O, Hamill R M, Davey G, McBryan J, Mullen A M, Gispert M,
Sweeney T (2011) Variation in the IGF2 gene promoter region is
associated with intramuscular fat content in porcine skeletal muscle.
Mol Biol Rep 39(4): 4101-4110.
Attia J, Thakkinstian A, D’Este C (2003) Meta-analyses of molecular
association studies: methodologic lessons for genetic epidemiology.
J Clin Epidemiol 56(4): 297-303.
Ateya A, Ibrahim S, Al-Sharif M (2022) Single nucleotide polymorphisms,
gene expression and economic evaluation of parameters
associated with mastitis susceptibility in European cattle breeds.
Vet Sci 9:294.
Ayele S A, Özcan B D, Bayraktar M (2024) Identification of the association
of the GH, IGF-I and PIT-I gene polymorphism with
growth traits in Saanen, Alpine and Boer goat breed. Small Rumin
Res 236:107297.
Bagnicka E, Siadkowska E, Strzałkowska N, Żelazowska B, Flisikowski
K, Krzyżewski J, Zwierzchowski L (2009) Association of polymorphisms
in exons 2 and 10 of the insulin-like growth factor 2 (IGF2)
gene with milk production traits in polish Holstein-Friesian cattle.
J Dairy Res 77(1): 37-42.
Berkowicz E W, Magee D A, Sikora K M, Berry D P, Howard D J, Mullen
M P, MacHugh D E (2010) Single nucleotide polymorphisms
at the imprinted bovine insulin-like growth factor 2 (IGF2) locus
are associated with dairy performance in Irish Holstein-Friesian
cattle. J Dairy Res 78:1–8.
Borenstein M (2023a) Avoiding common mistakes in meta‐analysis:
understanding the distinct roles of Q, I‐squared, tau‐squared, and the
prediction interval in reporting heterogeneity. Res Synth Methods
(2): 354-368.
Borenstein M (2023b) How to understand and report heterogeneity in
a meta-analysis: the difference between I-squared and prediction
intervals. Integr Med Res 12(4): 101014.
Chao W, D’Amore P A (2008) Igf2: Epigenetic regulation and role in
development and disease. Cytokine Growth Factor Rev 19:111–120.
Criado‐Mesas L, Ballester M, Crespo-Piazuelo D, Castelló A, Benítez
R, Fernández A I, Folch J M (2019) Analysis of porcine IGF2 gene
expression in adipose tissue and its effect on fatty acid composition.
PLoS One 14(8): e0220708.
Cullere M, Zotte A D (2018) Rabbit meat production and consumption:
state of knowledge and future perspectives. Meat Sci 143:137–146.
Fontanesi L, Mazzoni G, Bovo S, Frabetti A, Fornasini D, Dall’Olio S,
Russo V (2012) Association between a polymorphism in the IGF2
gene and finishing weight in a commercial rabbit population. Anim
Genet 43:651–652.
Hamad A A, Alkhawaldeh I M (2024) Beyond cochrane’s I²: diverse
methods for assessing heterogeneity in meta-analysis. La Tunisie
Méd 102(12).
Harris L K, Westwood M (2011) Biology and significance of signalling
pathways activated by IGF-II. Growth Factors 30:1–12.
Hatab A A, Cavinato M E R, Lagerkvist C J (2019) Urbanization, livestock
systems and food security in developing countries: a systematic
review of the literature. Food Secur 1 1:279–299.
Helal M, Hany N, Maged M, Abdelaziz M, Osama N, Younan YW,
Ragab M (2021) Candidate genes for marker-assisted selection for
growth, carcass and meat quality traits in rabbits. Anim Biotechnol
:1691–1710.
Hosnedlová B, Vernerová K, Kizek R, Bozzi R, Kadlec J, Čurn V,
Kouba F, Fernandez C, Machander V, Horna H (2020) Associations
between IGF1, IGFBP2, and TGFβ3 genes polymorphisms
and growth performance of broiler chicken lines. Animals 10:800.
Huang B, Khan M Z, Chai W, Ullah Q, Wang C (2023) Exploring
genetic markers: mitochondrial DNA and genomic screening for
biodiversity and production traits in donkeys. Animals 13:2725.
Huang S, Lo L, Chung MT, Li H, Tu C, Tsou H, Huang M, Yu Y, Huang
T, Lin E (2012) Effects of single-nucleotide polymorphisms in insulin-
like growth factor-1 and insulin-like growth factor-2 genes
on growth performance of centrally tested Duroc boars using segregated
early weaning entrance. Livest Sci 144:290–293.
Huang Y, Wang J, Zhan Z, Cao X, Sun Y, Lan X, Lei C, Zhang C, Chen H
(2013) Assessment of association between variants and haplotypes
of the IGF2 gene in beef cattle. Gene 528:139–145.
Huedo‐Medina T B, Sánchez‐Meca J, Marín‐Martínez F, Botella J (2006)
Assessing heterogeneity in meta-analysis: Q statistic or I² index?.
Psychol Methods 11(2): 193-206
Kpomasse C, Kouame Y, N’nanle O, Houndonougbo F M, Tona K, Oke
O (2023) The productivity and resilience of the indigenous chickens
in the tropical environments: improvement and future perspectives.
J Appl Anim Res 51:456-469.
Laere A V, Nguyen M Q, Braunschweig M, Nezer C, Collette C, Moreau
L, Archibald A, Haley C, Buys N, Tally M, Andersson G, Georges
M, Andersson L (2003) A regulatory mutation in IGF2 causes a
major QTL effect on muscle growth in the pig. Nature 425(6960):
-836.
LeRoith D, Holly J M, Forbes B E (2021) Insulin-like growth factors:
ligands, binding proteins, and receptors. Mol Metab 52:101245.
Li Z H, Li H, Zhang H, Wang S Z, Wang Q, Wang Y X (2006) Identification
of a single nucleotide polymorphism of the insulin-like
growth factor binding protein 2 gene and its association with growth
and body composition traits in the chicken. J Anim Sci 84(11):
-2906.
Li Z, Wu Z, Ren G, Zhao Y, Liu D (2012) Expression patterns of insulin-
like growth factor system members and their correlations with
growth and carcass traits in Landrace and Lantang pigs during
postnatal development. Mol Biol Rep 40:3569–3576.
Long J, Cai X (2007) IGF-2r expression regulated by epigenetic modification
and the locus of gene imprinting disrupted in cloned cattle.
Gene 388(1-2): 125-134.
Magotra A, Bangar Y C, Patil C, Sindhu V, Kumar D, Chaudhary P, Garg
A R, Kumar S (2023) Association of CXCR1 gene polymorphism
with clinical mastitis, reproductive disorders and performance traits
in Hardhenu (Bos taurus × Bos indicus) cattle. Reproduction in
Domestic Animals 58:1234–1243.
McDermott J J, Staal S J, Freeman H A, Herrero M, Steeg J V D (2010)
Sustaining intensification of smallholder livestock systems in the
tropics. Livest Sci 130:95–109.
Michalk D L, Kemp D, Badgery W, Wu J, Zhang Y, Thomassin P J (2018)
Sustainability and future food security—a global perspective for
livestock production. Land Degrad Dev 30:561-573.
Minelli C, Thompson J R, Abrams K R, Thakkinstian A, Attia J (2007)
How should we use information about HWE in the meta-analyses
of genetic association studies?. Int J Epidemiol 37(1): 136-146 .
Moher D, Liberati A, Tetzlaff J, Altman DG (2009) Preferred reporting
items for systematic reviews and meta-analyses: the PRISMA
statement. PLoS Med 6:e1000097.
Nurcahya H, Sinaga EJ, Darwati S, Khaerunissa I (2020) Relationship
between insulin-like growth factor-2 gene and growth traits in
crosses of Indonesian local chicken. J Phys Conf Ser 1665:012015.
Olaniyan O F, Oladejo O A, Adeola A C, Bello S F, Bashiru H A, Oseni
S O (2024) Integration of genomics into community-based breeding
programmes for chickens: an overview of opportunities, challenges,
and potential benefits. World Poult Sci J 80:977–997.
Pauciullo A, Gaspa G, Zhang Y, Liu Q, Cosenza G (2024) CSN1S1,
CSN3 and LPL: three validated gene polymorphisms useful for
more sustainable dairy production in the Mediterranean river buffalo.
Animals 14:1414.
Pedder H, Sarri G, Keeney E, Nunes V, Dias S (2016) Data extraction
for complex meta-analysis (DECiMAL) guide. Syst Rev 5:212.
R Core Team (2022) R: A language and environment for statistical computing.
Vienna, Austria: R Foundation for Statistical Computing.
Ramadan S, Manaa EA, El-Attrony M, Nagar AGE (2020) Association
of growth hormone (GH), insulin-like growth factor 2 (IGF2), and
progesterone receptor (PGR) genes with some productive traits in
Gabali rabbits. World Rabbit Sci 28:135.
Safaa HM, Ragab M, Ahmed M, El-Gammal B, Helal M (2023) Influence
of polymorphisms in candidate genes on carcass and meat quality
traits in rabbits. PLoS One 18:e0294051.
SPSS (2021) IBM Corp., IBM SPSS Statistics for Windows, Version
0, Armonk, NY, USA: IBM Corp.
Thakkinstian A, McElduff P, D’Este C, Duffy D L, Attia J (2005) A
method for meta-analysis of molecular association studies. Stat
Med 24(9): 1291-1306
Torrente Y, Bella P, Tripodi L, Villa C, Farini A (2020) Role of insulin-
like growth factor receptor 2 across muscle homeostasis: implications
for treating muscular dystrophy. Cells 9:441.
Viechtbauer W (2010) Conducting meta-analyses in R with the metafor
package. J Stat Softw 36:1–48.
Wallace BC, Dahabreh IJ, Trikalinos TA, Lau J, Trow P, Schmid CH
(2012) Closing the gap between methodologists and end-users: R
as a computational back-end. J Stat Softw 49:1–15.
Wang G, Yan B, Deng X, Changlu L, Hu X, Li N (2005) Insulin-like
growth factor 2 as a candidate gene influencing growth and carcass
traits and its biallelic expression in chicken. Sci China Life Sci
:187–194.
Wu Y, Lin S, Gu L, Lu L, Liao Z, Peng Q, Zhang L (2025) Research
note: detection of IGF2BP2 gene polymorphism associated with
growth and reproduction traits of pigeon. Poultry Sci 104:104834.
Xue Q, Zhang G, Li T, Ling J, Zhang X, Wang J (2017) Transcriptomic
profile of leg muscle during early growth in chicken. PloS One
(3): e0173824.
Zalewska M, Puppel K, Sakowski T (2021) Associations between gene
polymorphisms and selected meat traits in cattle—a review. Anim
Biosci 34:1425–1438.
Zhang Z, Li Q (2008) Association of polymorphism of IGF-II gene with
growth traits in Nanyang cattle. Front Biol:2008.