Comparison of the Vaginal Cytological and Microbiological Results in the Detection of Normal Microflora of Pregnant

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Pubblicato: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15474
E. S. ÖZDEMIR SALCI
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Uludag University
G. GONCAGÜL
Vocational School of Mennan Pasinli, Uludag University
V. İPEK
Department of Pathology, Faculty of Veterinary Medicine, Uludag University
Abstract

The aim of this study was to carry out a cytological and microbiological comparative investigation of vaginal microflora in pregnant ewes. The subjects for the study comprised of 39 healthy curly fleeced breed ewes (n=39), approximately 3 years old, at 2-4 months of pregnancy. Two vaginal samples were taken for cytological and microbiological examinations from each ewe in a sterile manner. Hemacolor® was used in cytological examination, while microbiological analysis were completed by conventional techniques. In cytological examination, slides were evaluated to detect lactobacilli, other bacteria, “clue cell” formation and presence of neutrophils. Microbiological investigation was carried out to detect possible pathogens. Cytological results compatible with bacterial vaginosis were obtained in 10 cases. Microbiologically, single type bacteria in 27 cases and more than one bacterium in 12 cases were isolated. The most common isolated pathogen was Escherichia coli. Comparing the cytological and microbiological results, 7 out of 27 cases were compatible with the bacterial vaginosis. In 3 cases of bacterial vaginosis non-pathogenic agents were revealed. In conclusion, it was proven that utilising the cytological examination provides more reliable results for detection of normal vaginal microflora of pregnant ewes.

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Riferimenti bibliografici
Demirezen S (2003) Review of cytologic criteria of bacterial vaginosis: examination of 2,841 Papanicolaou-stained vaginal smears. Diagn Cytopathol 29 (3): 156-159.
Doğaneli MZ, Tanyolaç A, Alaçam E (1979) Koyunlarda gebeliğin çeşitli evrelerinde vaginal smear ve vaginal biyopsi yöntemleriyle çalışmalar. Ankara Üniv Vet Fak Derg 26: 177-183.
El-Arabi AA, Taylor DJ, Logue DN, Benothman MJ (2013) Isolation and identification of bacterial flora from reproductive tracts of normal ewes in Glasgow. J Vet Adv 3(10): 275-280.
Gorga F, Galdiero M, Buommino E, Galdiero E (2001) Porins and lipopolysaccharide induce apoptosis in human spermatozoa. Clin Diagn Lab Immunol 8 (1): 206-208.
Larsen B, Monif GR (2001) Understanding the bacterial flora of the female genital tract. Clin Infect Dis 32 (4): 69-77.
Li J, McCormick J, Bocking A, Reid G (2012) Importance of vaginal microbe in reproductive health. Reprod Sci 19: 235-242.
Money D (2005) The laboratory diagnosis of bacterial vaginosis. Can J Infect Dis Med Microbiol 16 (2): 77-79.
O’Halan DE, Moench TR, Cone RA (2013) Vaginal pH and microbicidal lactic acid when lactobacilli dominate the microbiota. PLoS One 8:e80074. doi:10.1371/journal.pone.0080074.
Shallali AA, Homeida AM, Dafalla EA (1987) Effects of intrauterine infection by Staphylococcus aureus and Mycoplasma capricolum on the fertility of Nubian goats. Reprod Nutr Dev 27 (6): 999-1003.
Shallali AA, Hussein AM, Salih MM, Dafalla EA (2001) A preliminary report on bacteria isolated from the female genital tract of Sudanese sheep and goats. The Sudan J Vet Res 17, 55-63.
Swartz JD, Lachman M, Westveer K, O’Neill T, Geary T, Kott RW, Berardinelli JG, Hatfield PG, Thomson JM, Roberts A, Yeoman CJ (2014) Characterization of the vaginal microbiota of ewes and cows reveals a unique microbiota with low levels of lactobacilli and nearneutral pH. Front Vet Sci 1: 19.
Ungerfeld R, Silva L (2005) The presence of normal vaginal flora is necessary for normal sexual attractiveness of estrous ewes. Appl Anim Behav Sci 93 (3-4): 245-250.
Zaid NW (2009) Vaginal flora of Iraqi sheep and goats during different reproductive stages. Al-Anbar J Vet Sci 2 (1): 25-30.