Detection of Salmonella spp. from zoo animals in Iran, determination of serovars, antibiotic susceptibility and genotyping by RAPD-PCR

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Pubblicato: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15494
B. N. FASAEI
Department of Microbiology and Immunology, Faculty of Veterinary Medicine
I. A. TAMAI
Faculty of Veterinary Medicine, University of bu-ali sina
Abstract

Salmonellosis is an important food-borne bacterial zoonotic disease that affects both people and animals. Contamination sources include direct or indirect animal contact. We determined and measured the risk of Salmonella infection from zoo animal to human contact by isolation of Salmonella serovars from zoo animals kept at Karaj zoo park, Iran. Salmonella was isolated from 21 (20%) of the 104 anal swap samples. From the 21 collected samples 4, 7 and 10 were originated from birds, mammals and reptiles, respectively. Serotyping detected nine different serotypes including Enteritidis (n=4), Seftenberg (n=1), Typhimurium (n=4), Virchow (3), Berkeley (n=1), Kingabwa (n=2), Newport (n=2), Marina (n=2) and Havana (n=1). All the isolates except one (serovar Marina subspecies Hautenae IV) were belonged to Salmonella enterica subspecies enterica I. Salmonella enterica serotypes Typhimurium and Enteritidis were the most commonly detected serotypes. All the isolates tested, were resistant to one or more antibiotics and five isolates including the monkeys’ and long-eared owls’ isolates were multiresistant. RAPD profiles of each isolate produced with two different primers were identical. These finding shows highly contamination of zoo animals from Salmonella especially from the multiresistant isolates. All of the animals in the zoo, pet owners, veterinarians, zookeepers and visitors are at high risk of salmonellosis with reptiles being the most important source of infection.

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Riferimenti bibliografici
Abalem IV and Solari CA (2001) Salmonella in Brazilian and imported pet reptiles. Brazil J Microbiol 32: 293-297.
Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk metod. Amer J Clin Patho 45: 493-496.
Campbell JV, Mohle-Boetani J, Reporter R, Abbott S, Farrar J, Brandl M, Mandrell R, Werner SB (2001) An outbreak of Salmonella serotype Thompson associated with fresh cilantro. J Infec Dis 183:984-987.
Corrente M, Madio A, Friedrich KG, Greco G, Desario C, Tagliabue S, Dincau M, Campolo M, Buonavoglia C (2004) Isolation of Salmonella strains from reptile faeces and comparison of different culture media. J Appl Microbiol 96: 709-715.
Ebani VV, Cerri D, Fratini F, Meille N, Valentini P, Andreani E (2004) Salmonella enterica isolates from faeces of domestic reptiles and a study of their antimicrobial in vitro sensitivity. Res Vet Sci 78:117-121.
Forshell LP and Wierup M (2006) Salmonella contamination: A significant challenge to the global marketing of animal food products. Revue Scientific et Technique de L,Office International Des Epizooties. 25: 541-554.
Franciscus ST, Lightfoot D, Holz P (2011) Prevalence of Salmonella in Australian reptiles. J WildL Dis 47: 1-11.
Friedman CR, Torigian C, Shillam PJ, Hoffman RE, Heltze LD, Beebe JL, Malcolm G, Dewitt WE, Hutwagner L, Griffin PM (1998) An outbreak of salmonellosis among children attending a reptile exhibit at a zoo. J Pediatr 132: 802-807.
Galan JE, Ginocchio C, Costeas P (1992) Molecular and Functional characterization
of the Salmonella invasion gene invA: homology of invA to members of a new protein family. J Bacteriol 174: 4338-4349.
Jang YH, Lee SL, Lim JG, Lee HS, Kim TJ, Park JH, Chung BH, Choe NH (2008) The rate of Salmonella spp. Infection in zoo animals at seoul grand park, Korea. J Vet Sci 9: 177-181.
Jardine C, Reid-Smith RJ, Janecko N, Allan M, McEwen SA (2011) Salmonella in raccoons (procyonlotor) in southern Ontario, Canada. J wildL Dis 47: 344-351.
Lin AW, Usera MA, Barrett TJ, Godsby RA (1996) Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis. J Clin Microbiol 34: 870-876.
Madadgar O, Zahraeisalehi T, Ghafari MM, AshrafiTamai I, Madani SA, Yahyareyat R (2009) Study of anusual paratyphoidepornitic in canaries (Serinuscanaria). Av Pathol 38: 437-441.
Malorny B, Hoofar J, Bunge C, Helmuth R (2003) Multicenter validation of the analytical accuracy of Salmonella PCR towards international standard. Appl Environ Microbiol 69: 590-596.
Mitchell MA, Shane SM, Nevarez J, Pesti D, Sanchez S, Wooley RE, Ritchie B (2001) Establishing a Salmonella-free iguana, Iguana iguana, model using enrofloxacin. In: Proceedings of the VIII annual conference, association of reptilian and amphibian reptiles. pp. 189-190.
Nakadai A, Kuroki T, Kato Y, Suzuki R, Yamai S, Yaginuma C, Shiotani R, Yamanouchi A, Hayashidani H (2005) Prevalence of Salmonella spp. in pet reptiles in Japan. J Vet Med Sci. 67: 97-101.
Neera VG, Abiodun AA, Kenneth C (2000) Retrospective and longitudinal study of salmonellosis in captive wilelife in Trinidad. J wildL Dis 36: 284-293.
Okoh AEJ and Onazi M (1980) Notes on salmonella isolated from wildlife in Kano zoological gardens. J wildL Dis 16: 7-10.
Olsen SJ, Bishop R, Bernner FW, Roels TH, Bean N, Tauxe RV, Slutsker L (2001) The changing epidemiology of Salmonella: trend in serotypes isolated from humans in the United states. J Infect Dis 183: 753-761.
Pedersen K, Lassen-Nielsen AM, Nordentoft S, Hammer AS (2008) Serovars of Salmonella from captive reptiles. Zoonoses Public Health 56: 238-242.
Pitout JD, Reisbig MD, Mulvey M, Chui L, Louie M, Crowe L, Church L, Elsayed S, Gregson D, Ahmed R, Tilley P, Hanson ND (2003) Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC betalactamase, CMY-2. J Clin Microbiol 41: 4578-4582.
Popoff MY and Le Minor L (1992) Antigenic formulas of the Salmonella serovars.6th ed. WHO collaborating centre for Reference and Research on Salmonella. Institute Pasteur Paris, France.
Quinn PJ, Carter ME, Markey PK, Carter GR (1994) Enterobacteriaceae. In: clinical veterinary Microbiology. pp 209-236, 2nd ed. London: Wolfe publishing.
Stephen J, Swanson MD, Cynthia Snider MHS, Christopher R, Braden MD (2007) Multidrug-resistant Salmonella enterica serotype Typhimurium associated with pet rodents. New England J Med 356, 21-28.
Tamimi N, Rostami A, Majidzadeh K, Bahonar A, Esmaeili H, Niazi shahraki S. The survey of Salmonella infection in pet reptiles in Tehran and the associated risk factors in their owners Iranian J Epidemiol 10(2): 24-32.
Tizard I (2004). Salmonellosis in wild birds. Seminars in avian and exotic pet medicine. 13: 50-56.
Zahraei Salehi T, Mahzounieh M, Khaksar E (2010) Detection of Salmonella serovars in zoo and pet reptiles, rabbits, and rodents in Iran by culture and PCR methods. Comp Clin Pathol 19: 199–202
Zahraei salehi T, AskariBadouei M, Madadgar O, Ghiasi SR, AshrafiTamai I (2013) Shepherd dogs as a common source for Salmonella enterica serovar Reading in Garmsar, Iran. Turk J Vet Sci 37: 102-10