Determination of Mycoplasma bovis specific antibodies in blood sera of asymptomatic carriers-calves in three farms in the Republic of Serbia by using indirect ELISA assay

PDF (English)
Pubblicato: Dec 21, 2017
DOI: https://doi.org/10.12681/jhvms.15518
D. VOJINOVIĆ
Scientific Veterinary Institute of Serbia-Belgrade
A. VASIĆ
Faculty of Veterinary Medicine, University of Belgrade
J. ŽUTIĆ
Scientific Veterinary Institute of Serbia-Belgrade
B. DURIČIĆ
Faculty of Veterinary Medicine, University of Belgrade
Z. ILIĆ
Scientific Veterinary Institute of Serbia-Belgrade
D. JOVIČIĆ
Faculty of applied ecology, Futura University
M. E. RADOVANOVIĆ
Faculty of Veterinary Medicine, University of Belgrade
Abstract

Blood serum samples of asymptomatic carriers-calves were collected from three farms in the territory of the Republic of Serbia during 2011 and 2012. Commercial Mycoplasma bovis ELISA kit (Bio-X Diagnostics, Belgique) for serological diagnosis from cattle blood sera and milk was used in this research. Calves’ blood sera were tested using immunoenzymatic indirect ELISA assay as described by manufacturer’s instructions. From 5603 blood sera of asymptomatic carriers-calves 144 (2,57%) samples were tested positive for the presence of specific Mycoplasma bovis antibodies. In three different farms proportions of seropositive samples varied from 0,32% to 10,6% in regard to total number of tested samples from the individual farms. In this paper we present the results of Mycoplasma bovis prevalence in asymptomatic carriers-calves.

Article Details
  • Sezione
  • Research Articles

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
I dati di download non sono ancora disponibili.
Riferimenti bibliografici
Arcangioli MA, Chazel M, Sellal E, Botrel MA, Bezille P, Poumarat F, Calavas D, Le Grand D (2011) Prevalence of Mycoplasma bovis udder infection in dairy cattle:prelininary field investigation in southeast France. NZ Vet .J 59:75-78.
Arcangioli MA, Froux W, Poumarat F, Le Grand D (2012) Case report: Mycoplasma bovis otitis in French dairy calves, Revue Med Vet 163: 284-286.
Ayling RD, Bashiruddin SE, Nicholas RAJ (2004) Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec 155, 413-416.
Byrne WJ, McCormac R, Brice N, Egan J, Markey B, Ball HJ (2001) Isolation of Mycoplasma bovis from bovine clinical samples in the Republic of Ireland. Vet Rec 148, 331-333.
Fu J-H, Liu Q-Y, Xu M-J, Shi D-S, He X-H, Pan Y, Guo R-B, Gao Q, Yi S-X, Si H-S, Zhu X-Q (2011) Seroprevalence of Mycoplasma bovis infection in dairy cows in subtropical southern China. Afr J Biotechnol 10, 11313-11316.
Gagea M., Bateman K., Shanahan R., Dreumel T., McEwen B., Carman S., Archambault M., Caswell J. (2006) Naturally occuring Mycoplasma bovis-Associated Pneumonia and polyarthritis in feedlot beef calves. J Vet Diagn Inv 18, 29.
Gonsales RN, Jayarao BM, Oliver SP, Sears PM (1993) Pneumonia, arthritis and mastitis in dairy cows due to Mycoplasma bovis. Proceedings of 32nd Annual Meeting of the National Mastitis Council, pp. 178-186.
Gourlay RN, Howard CJ, Thomas LH, Stott EJ (1976) Experimentally produced calf pneumonia. Res Vet Sci 20, 167.
Gourlay RN, Thomas LH (1969) Experimental pneumonia in calves produced by inoculation of mycoplasmas. Vet Rec 85, 583.
Hale HH, Helmboldt CF, Plastridge WN, Stula EF, (1962) Bovine mastitis by Mycoplasma species Cornell Vet 52, 582-591.
Le Grand D, Bezille P, Calavas D, Poumarat F, Brank M, Citti C, Rosengarten (2002) Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France Vet Rec 150, 268-273.
Levisohn S, Garazi S, Gerchmann I, Brenner J (2004) Diagnosis of a mixed mycoplasma infection associated with a severe outbreak of bovine pinkeye in young calves. J Vet Diagn Invest 16, 579-581.
Maeda T, Shibahara T, Kimura K, Wada Y, Sato K, Imada Y, Ishikawa Y, Kadota K (2003) Mycoplasma bovis-associated suppurative otitis media and pneumonia in bull calves. J Comp Pathol 129, 100-110.
Nicholas RA (2004) Recent developments in the diagnosis and control of mycoplasma infection in catlle. Proceedings of 23rd World Buiatrics Congress Quebec, Canada, July 11-16,, 2004.
Nicholas RA, Ayling RD, Woodger N, Wessells ME, Houlihan MG (2006) Mycoplasmas in adult cattle: bugs worth bothering about? Ir Vet J 59, 568-572.
Pfutzner H (1990) The control of the Mycoplasma infections in bulls. IOM Letters 1, 505-506.
Pfutzner H, Sachse K (1996) Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Rev Sci Tech Off Int Epiz 15, 1477-1494.
Razin S (1978) The mycoplasmas. Microbiol Rev 42, 414-470.
Tambuwal FM, Stipkovits L, Egwu GO, Junaidu AU, Abubakar MB, Turaki UA (2011) Enzyme-linked immunosorbent assay (Elisa) based detection of antibodies to Mycoplasma bovis in cattle naturally infected with haemoparasites in institutional farms in Sokoto state, Nigeria. Curr Res J Biol Sci 3, 12-16.
Tenk M (2005) Examination of Mycoplasma bovis Infection in Cattle. PhD thesis, Budapest Szent Istvan University. Tenk M, Stipkovits L, Hufnagel L (2004) Examination of the role of Mycoplasma bovis in bovine pneumonia and a mathematical model for its evalation. Acta Vet Hung 52, 445-456.
Wiggins MC, Woolums AR, Hurley DJ, Sanchez S, Ensley DT, Donovan D (2011) The effect of various Mycoplasma bovis isolates on bovine leukocyte responses. Comp Immunol Microbiol Infect Dis 34, 49-54.
Zhao FR, Zhu XL, Xu MJ, Huang SY, Zhou DH, Xia HY, Song HQ, Zou FC (2012) Afr J Microbiol Res 6, 668-5671.
Puoi leggere altri articoli dello stesso autore/i