Antimicrobial resistance profile and resistance genes of Vibrio species isolated from giant freshwater prawn (Macrobrachium rosenbergii) raised in Iran

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Pubblicato: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15566
A. SHAKERIAN
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University; School of Pharmacy and Medical Sciences, University of South Australiaia
M. D. BARTON
School of Pharmacy and Medical Sciences, University of South Australia
O. L. AKINBOWALE
School of Pharmacy and Medical Sciences, University of South Australia
F. KHAMESIPOUR
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences; Young Researchers and Elite, Shahrekord Branch, Islamic Azad University
Abstract

Because of raising of large-scale high density prawn aquaculture techniques, production of this prawn worldwide is facing a serious threat from fatal diseases caused by nodaviruses and bacteria, particularly from the Vibrio species. The development of antibiotic resistance by Vibrio represents a potential threat to human health by exchange of resistant genes to human pathogens through food chain. This study aimed to determine antibiotic resistance profile of Vibrio isolates from giant fresh water Prawns (Macrobrachium rosenbergii) raised in Iran and to detect some antibiotic resistance genes in the isolates. A total of fifty giant fresh water prawns were processed for isolation of Vibrio species during February 2015 to August 2015. Identification of Vibrio isolates was done following standard biochemical methods. Phenotypic resistance of the isolates as determined by agar dilution method while polymerase chain reaction (PCR) method was used to detect the presence of erm, tetS, strA and sul2 genes in the isolates. Out of 50 prawns, 31 (62%) isolates of Vibrio spp. were reported, of which 20 (40%) were identified as V. parahaemolyticus, 10 (20%) were V. vulnificus and 1 (2%) were V. cholera. Over 90% of the tested strains showed susceptibility to SXT, AZM and NIT. In addition, strA and tetS genes were detected in all isolated Vibrio species. StrA gene was identified in 6 V. parahaemolyticus strains and also ermB and sul2 genes were not present in the isolate of V. cholera. The occurrence of multidrug resistance strains in the environment could be an indication of excessive usage of antibiotics in agriculture and aquaculture fields. This study has shown that giant freshwater prawns raised in Iran habour multidrug resistant Vibrio species.

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Riferimenti bibliografici
Akinbowale OL, Peng H, and Barton MD (2007) Diversity of tetracycline resistance genes in bacteria from aquaculture sources in Australia. Journal of Applied Microbiology 103: 2016-2025.
Akinbowale OL, Peng H, and Barton MD (2006) Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. Journal of Applied Microbiology 100: 1103-1113.
Al-Othrubi SM, Kqueen CY, Mirhosseini H, H adi YA, and Radu S (2014) Antibiotic resistance of Vibrio parahaemolyticus Isolated from cockles and shrimp sea food marketed in Selangor, Malaysia. Clinical Microbiology 3: 148-154.
Angulo FJ (2000) Antimicrobial agents in aquaculture: potential impact on health. APUA Newsletter. 18: 1-6.
Ausubel FM, Brent R, Kingston RE, Moore DD, Sideman J, Smith J, and Struhl K (1987) Current Protocols in Molecular. Biology, USA: Wiley-Blachwell, p. 354.
Bani S, Mastromarino PN, Ceccarelli D, Le Van A, Salvia AM, Ngo Viet QT, Hai DH, Bacciu D, Cappuccinelli P, and Colombo MM (2007) Molecular characterization of ICEVchVie0 and its disappearance in Vibrio cholerae O1 strains isolated in 2003 in Vietnam. FEMS Microbiology Letters 266: 42-48.
Bonami J-R, and Sri WJ (2011) Viral diseases of the giant fresh water prawn Macrobrachium rosenbergii: a review. Journal of Invertebrate Pathology 106 (1): 131-142.
Cabello FC,Godfrey HP,Tomova A,Ivanova L,Dölz H, Millanao A, and Buschmann AH (2013) Antimicrobial use in aquaculture reexamined: its relevance to antimicrobial resistance and to animal and human health. Applied and Environmental Microbiology 15:1917-1942.
CLSI (2010) Performance standards for antimicrobial susceptibility testing; methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria (Approved standard (M45-A)). Wayne, PA: Clinical and Laboratory
Standards Institute.
Dang H, Zhang X, Song L, Chang Y, and Yang G (2006) Molecular characterizations of oxytetracycline resistant bacteria and their resistance genes from mariculture waters of China. Marine Pollution Bulletin 52: 1494-1503.
Dang H, Zhang X, Song L, Chang Y, and Yang G (2007) Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China. Journal of Applied Microbiology 103: 2580-2592.
Dang HY, Ren J, Song LS, Sun S, and An LG (2008) Domi- nant chloramphenicol resistant bacteria and resistance genes in coastal marine water sof Jiazhou Bay, China. World Journal of Microbiology and Biotechnology 24: 209-217.
Daniels NA, MacKinnon L, Bishop R, Altekruse S, Ray B, Hammond RM, Thompson S, Wilson S, Bean NH, Griffin PM, and Slutsker L (2000) Vibrio parahaemolyticus infection in the UnitedStates,1973-1998. The Journal of Infectious Diseases 181:1661-1666.
De Grave S, Cai Y, and Anke rA (2008) Global diversity of shrimps (Crustacea: Decapoda: Caridea) in freshwater. Hydrobiologia 595:287-293.
Duran GM, and Marshall DL (2005): Ready-to-eat shrimp as international vehicle of antibiotic-resistant bacteria. Journal of Food Protection 68: 2395-2401.
García-Aljaro C, Riera-Heredia J, Blanch AR (2014) Antimicrobial resistance and presence of the SXT mobile element in Vibrio spp. isolated from aquaculture facilities. New Microbiologica 37: 339-346.
Goel AK, Jain M, Kumar P, and Jiang SC (2010) Molecular characterization of Vibrio cholerae outbreak strains with altered El Tor biotype from southern India. World Journal of Microbiology and Biotechnology 26: 281-287.
Grant MA, Hu J, and Jinneman KC (2006) Multiplex real-time PCR detection of heat labile and heat-stable toxingenes in enterotoxigenic Escherichia coli. Journal of Food Protection 69:412-416.
Guglielmetti E, Korhonen JM, Heikkinen J, Morelli L, and Von Wright A (2009) Transfer of plasmid- mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments. FEMS Microbiology Letters 293: 28-34.
Han F, Walker RD, Janes ME, Prinyawinwatkul W, and Ge B (2007) Antimicrobial susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus isolates from Louisiana Gulf and retail raw oysters. Applied and Environmental Microbiology 73: 7096-7098.
Hemmatinezhad B, Khamesipour F, Mohammadi M, Safarpoor Dehkordi F, Mashak Z (2015) Microbiological investigation of O-Serogroups, virulence factors and antimicrobial resistance properties of Shiga toxin-producing Escherichia coli isolated
from ostrich, turkey and quail meats. Journal of Food Safety 35:491–500.
Khamesipour F, Noshadi E, and Moradi M (2014) Detection of the prevalence of Vibrio spp. in shrimp samples by Polymerase Chain Reaction (PCR) and cultural method in the Iran. AACL BIOFLUX. 7(1): 1-7.
Khamesipour F, Doosti A, Koohi A, Chehelgerdi M, Mokhtari-Farsani A, Chengula AA (2015) determination of the presence of Babesia DNA in blood samples of cattle, camel and sheep in iran by PCR. Archives of Biological Science Belgrade 67 (1): 83-90.
Khodadadi M, Hemmatinezhad B, Doosti A, Khamesipour F, Awosile B (2015) Molecular detection and prevalence of Chlamydophila psittaci in the blood, liver and muscle tissue of urban pigeons (Columba livia domestica) in Iran. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21 (2): 265-269.
Khuntia CP, Das BK, Samantaray BR, Samal SK, and Mishra BK (2008) Characterization and pathogenicity studies of Vibrio parahaemolyticus isolated from diseased freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research 39(3): 301-10.
Kim M, Kwon TH, Jung SM, Cho SH, Jin SY, Park NH, Kim CG, and Kim JS (2013) Antibiotic resistance of bacteria isolated from the internal organs of edible snow crabs. PLoSONE 8: 70887.
Laganà P, Caruso G, Minutoli E, Zaccone R, and Santi D (2011) Susceptibility to antibiotics of Vibrio spp. and Photobacterium damsela ssp. Piscicida strains isolated from Italian aquaculture farms. New microbiologica 34: 53-63.
Lajnef R, Snoussi M, Romalde JL, Nozha C, and Hassen A (2012) Comparative study on the antibiotic susceptibility and plasmid profiles of Vibrio alginolyticus strains isolated from four Tunisian marine biotopes. World Journal of Microbiology and Biotechnology 28: 3345-3363.
Macauley JJ, Adams CD, and Mormile MR (2007) Diversity of tet resistance genes in tetracycline resistant bacteria isolated from a swine lagoon with low antibiotic impact. Canadian Journal of Microbiology 53: 1307-1315.
Mala E, Oberoi A, and Alexander VS (2014) Vibrio isolates from cases of acute diarrhea and their antimicrobial susceptibility pattern in a tertiary care hospital. International Journal of Basic and Applied Sciences 3: 35-37.
Malcolm TTH, Cheah YK, Radzi CWJWM, Kasim FA, Kantilal HK, John TYH, Martinez-Urtazaf J, Nakaguchig Y, Nishibuchig M, and Son R (2015) Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay. Food Control 47:664-671.
Manjusha S, and Sarita GB (2011) Plasmid associated antibiotic resistance in Vibrio isolated from coastal waters of Kerala. International Food Research Journal 18: 1171-1181.
New MB (2002) Farming freshwater prawns: a manual for the culture of the giant river prawn (Macrobrachium rosenbergii): Food and Agriculture Organization of the United Nations 148: 1-11.
Okuda J, Ishibashi M, Hayakawa E, Nishino T, Takeda Y, Mukhopadhyay AK, Garg S, Bhattacharya SK, Nair GB, and Nishibuchi M (1997) Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta, India and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan. Journal of Clinical Microbiology 35: 3150–3155.
Panicker G, Myers ML, and Bej AK (2004) Rapid detection of Vibrio vulnificus in shell fish and Gulf of Mexico water by real-time PCR. Applied and Environmental Microbiology 70: 498-507.
Paydar M, Teh CSJ, and Thong KL (2013) Prevalence and characterization of potentially virulent Vibrio parahaemolyticus in seafood in Malaysia using conventional methods, PCR and REP-PCR. Food Control 32: 13-18.
Rahimi E, Khamesipour F, Yazdi F, Momtaz H (2012) Isolation and characterization of enterohaemorragic Escherichia coli O157:H7 and EHEC O157:NM from raw bovine, camel, water buffalo, caprine and ovine milk in Iran. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 18: 559-64.
Raissy M, Khamesipour F, Rahimi E, and Khodadoostan A (2014) Occurrence of Vibrio, Aeromonas hydrophila, E. coli and Campylobacter in crayfish (Astacus leptodactylus) from Iran. Iranian Journal of Fisheries Sciences 13(4): 944- 954.
Raissy M, Moumeni M, Ansari M, and Rahimi E (2012) Antibiotic resistance pattern of some Vibrio strains isolated from seafood. Iranian Journal of Fisheries Sciences 11(3): 618-626.
Ramesh A, Loganathan B, Venkateswaran K (1990) Ecological dynamics of marine luminous bacteria. Journal of Basic Microbiology 30(9): 689-703.
Rico A, Satapornvanit K, Haque MM, Min J, Nguyen PT, Telfer T, and van den Brink PJ (2012) Use of chemicals and biological productsin Asian aquaculture and their potential environmental risks: a critical review. Reviews in Aquaculture 4: 75-93.
Roque A, Molina-Aja A, Bolan-Mejia C, and Gomez-Gil B (2001) In vitro susceptibility to 15 antibiotics of vibrios isolated from penaeid shrimps in Northwestern Mexico. International Journal of Antimicrobial Agents 17: 383-387.
Rosser SJ, and Young HK (1999) Identification and characterization of class 1 integrons in bacteria from an aquatic environment. Journal of Antimicrobial Chemotherapy 44: 11-18.
Ruangpan L, Kitao T (1991) Vibrio bacteria isolated from black tiger shrimp, Penaeus monodon Fabricius. Journal of Fish Diseases 14(3): 383-388.
Serrano PH (2005) Responsible use of antibiotics in aquaculture. In: Food and agriculture organization (FAO). Fisheries Technical Paper 469, Roma, 97.
Shaw KS, Rosenberg GRE, He X, Jacobs JM, Crump BC, and Sapkota AR (2014) Antimicrobial susceptibility of Vibrio vulnificus and Vibrio parahaemolyticus recovered from recreational and commercial areas of Cheaspeake Bay and Maryland coastal bay. PLoSONE 9: e89616.
Snoussi M, Chaieb K, Rouabhia M, and Bakhrouf A (2006) Quantitative study, identification and antibiotics sensitivity of some Vibrio naceae associated to a marine fish hatchery. Annals of Microbiology 56: 289-293.
Snoussi M, Hajlaoui H, Noumi E, Zanetti S, and Bakhrouf A (2008) Phenotypic and molecular characterization of Vibrio alginolyticus strains recovered from juveniles and older Sparus aurata reared in a Tunisian marine farm. Annals of Microbiology 58: 141-146.
Snoussi M, Noumi E, Lajnef R, Belila A, Yazidi N, and Bakhrouf A (2011) Phenotypic characterization and enterobacterial repetitive intergenic consensus polymerase chain reaction (ericpcr) of Aeromonas spp. and Vibrio spp. strains isolated from Sparus aurata fish farm (Khenis, Tunisia). African Journal of Microbiology Research 5: 2920-2928.
Solati SM, Tajbakhsh E., Khamesipour F, Gugnani HC (2015) Prevalence of virulence genes of biofilm producing strains of Staphylococcus epidermidis isolated from clinical samples in Iran. AMB Express 5, 47.
Srinivasan V, Nam HM, Nguyen LT, Tamilselvam B, Murinda SE, and Oliver SP (2005) Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathogens and Disease 2: 201-211.
Sudha S, Mridula C, Silvester R, and Hatha AAM (2014) Prevalence and antibiotic resistance of pathogenic Vibrios in shellfishes from Cochin market. Indian Journal of Geo-Marine Sciences 43: 815-824.
Tajbakhsh E, Tajbakhsh S, Khamesipour F (2015) Isolation and molecular detection of Gram negative bacteria causing urinary tract infection in patients referred to Shahrekord hospitals, Iran. Iranian Red Crescent Medical Journal 17(5): e24779.
Taneja N, Samanta P, Mishra A, and Sharma M (2010) Emergence of tetracycline resistance in Vibrio cholerae O1 biotype El Tor serotype Ogawa from north India. Indian Journal of Pathology and Microbiology 53: 865-866.
Tang HJ,Chang MC, Ko WC, Huang KY, Lee CL, and Chuang YC (2002) In vitro and in vivo activities of newer fluoroquinolones against Vibrio vulnificus. Antimicrobial Agents and Chemotherapy 46: 3580-3584.
Tendencia E, and de la Pena L (2001) Antibiotic resistance of bacteria from shrimp ponds. Aquaculture 195: 193-204.
Thompson FL, Iida T, and Swings J (2004) Biodiversity of vibrios. Microbiology and Molecular Biology Reviews 68(3): 403-431.
Tonguthai K (1995) Diseases of the freshwater prawn, Macrobrachium rosenbergii. The Aquatic Animal Health Research Institute 4: 1-4.
Ture M, and Boran H (2015) Phenotypic and genotypic antimicrobial resistance of Lactococcus sp. strains isolated from rainbow trout (Oncorhynchus mykiss). Bulletin of the Veterinary Institute in Pulawy 59: 37-42.
Vaseeharan B, Ramasamy P, Murugan T, and Chen JC (2005) In vitro susceptibility of antibiotics against Vibrio spp. And Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. International Journal of Antimicrobial Agents 26: 285-291.
Xu B, Xu H, Ji W, and Shi J (1994) Pathogens and pathogenicity to Penaeus orientalis Kishinouye. Acta Oceanologica Sinica 13(2):297-304.
Yamamoto A, Iwahori J, Vuddhakul V, Charernjiratrag W, Vose D, Osaka K, Shigematsu M, Toyofuku H, Yamamoto S, Nishibuchi M, and Kasuga F (2008) Quantitative modeling for risk assessment of Vibrio parahaemolyticus in bloody clams in southern Thailand. International Journal of Food Microbiology 124: 70-78.
YanoY, Hamano K, Satomi M, Tsutsui I, Ban M, and Aue-umneoy D (2014) Prevalence and antimicrobial susceptibility of Vibrio species related to food safety isolated from shrimp cultured a tin land ponds in Thailand. Food Control 38: 30-45.
Zhang XX, Zhang T, and Fang HHP (2009) Antibiotic resistance genes in water environment. Applied Microbiology and Biotechnology 82: 397-414.
Zhang Z, Xiao L, Lou Y, Jin M, Liao C, Malakar PK, Pana Y, AND Zhaoa Y (2014) Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus, Listeria monocytogenes and Salmonella spp. In raw shrimp. Food Control 51: 31-36.